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56results about How to "Does not affect normal growth and development" patented technology

Mouse-typhus salmonella gene-deletion mutant strain without containing resistance marks, vaccine and application thereof

The invention relates to a mouse-typhus salmonella gene-deletion mutant strain without containing resistance marks, a vaccine and application thereof, belonging to the technical field of animal bacterium genetic engineering. The invention utilizes a mediated allelic exchange technique of recombinant suicidal plasmid for obtaining the mouse-typhus salmonella gene-deletion mutant strain (Salmonellatyphimurium SL13441) (the preservation number is CCTCC NO: M2010301) without containing the resistance marks. The mutant strain is deficient in an important toxicity regulating gene-cya gene in mouse-typhus salmonella, so that the toxicity of the mouse-typhus salmonella is obviously weakened after the deficiency of the cya gene, but the mouse-typhus salmonella still has immunogenicity. The invention also discloses the Salmonella gullinarum gene-deletion vaccine prepared by utilizing the gene-deletion mutant strain and an application of the gene-deletion mutant strain in preparing the vaccine, and the prepared vaccine meets the requirement on biological safety. The gene-deletion vaccine prepared in the invention can effectively prevent infection of Salmonella gullinarum and has the advantages of good stability and safety and low cost, is convenient to use and has obvious economic and social benefits.
Owner:HENAN UNIV OF SCI & TECH

Simple and efficient field inoculation method for rice false smut germ

The invention belongs to the technical field of plant protection and particularly relates to a simple and efficient field inoculation method for a rice false smut germ. The method comprises the steps: picking rice false smut balls from rice ears in a rice field at a rice ripening period in last year, drying the rice false smut balls in the air indoors, loading the dried rice false smut balls into plastic bags or plastic bottles, and placing the plastic bags or plastic bottles ay an indoor shady place; and at a 3-4 stage of young panicle differentiation of rice, putting the rice false smut balls preserved in last year into clean water, carrying out soaking for 3-5 hours, then, carrying out oscillating so as to enable rice false smut powder on the outside surface of each rice false smut ball to gradually fall off until rice grains are clearly discernible, then, enabling the rice false smut powder to be completely scattered into water, carrying out filtering with gauze so as to filter off grain residues and other foreign bodies, then, pouring filter liquor into a sprayer, adjusting water volume according to a spraying area, directly spraying the filter liquor to fields at 10 am to 5 pm in a sunny day, and swinging the sprayer during spraying so as to achieve uniform spraying as far as possible. By adopting the method disclosed by the invention, the onset ear ratio of field artificial inoculation of rice false smut can reach about 30% and is extremely stable. The method disclosed by the invention is extremely simple and can be used for carrying out field identification on degree of disease resistance to rice false smut of rice varieties without special utensils and conditions.
Owner:JIANGSU COASTAL AREA AGRI SCI RES INST

Disease prevention method of increasing content of phenolic substance in strawberry fruit

The invention discloses a disease prevention method of increasing the content of phenolic substance in strawberry fruit, and belongs to the technical field of crop cultivation. The method comprises the steps that the strawberry plants are UV-C irradiation treated once every 2-4 days beginning from the flowering period and continuing until the fruiting period; the conditions for the UV-C irradiation treatment are the following: the irradiation dosage amount is less than 0.75 kJ/m2, the irradiation time is less than 70 s; after the UV-C irradiation treatment is completed, the strawberry plants need to be shaded from light for larger than or equal to 8 hours. According to the disease prevention method, the strawberry plants are regularly UV-C irradiated in the flowering and fruiting period, the contents of anthocyanin and flavonol and other phenolic substances in strawberry fruits are significantly increased, meanwhile the antioxidant ability of the fruits is increased; the low dosage UV-C irradiation combined with light shading treatment method is utilized, physiological metabolisms related to the course of disease of the strawberry plants are induced, the anti-disease ability of the strawberry plant per se is effectively increased, and the incidence rate of the natural diseases of the strawberries is significantly decreased.
Owner:ZHEJIANG UNIV

Maintenance method for garden landscape lawn

The invention provides a maintenance method for a garden landscape lawn. The maintenance method comprises the following steps: (1) laying plant ash onto the surface layer of soil when a lawn is established, then using salicylic acid to prepare a salicylic acid aqueous solution with a concentration of 0.5 to 1 mg/L, spraying the salicylic acid aqueous solution onto the plant ash, and sowing grass seeds in 5 to 7 days after the salicylic acid aqueous solution is sprayed; (2) uniformly mixing salicylic acid, citric acid, polyacrylamide, ferrous sulfate and magnesium sulfate with water so as to obtain a mixed solution which is used right after prepared, and respectively spraying the mixed solution once to the lawn before the lawn turns green in spring and before the lawn is withered and turnsyellow in autumn; and (3) uniformly mixing uniconazole, naphthaleneacetic acid, abscisic acid, ferrous sulfate and potassium dihydrogen phosphate with water so as to obtain a mixed solution which is used right after prepared, spraying the mixed solution once to a vigorously-growing cold-season lawn in spring, and spraying the mixed solution once to a vigorously-growing warm-season lawn in midsummer. The maintenance method provided by the invention has the advantages of preventing weeds from surviving, significantly increasing the content of chlorophyll and greatly reducing the number of timesof lawn trimming.
Owner:ZHEJIANG NORMAL UNIVERSITY

Method for improving Guangzhou cherry blossom and China Red cherry blossom hybridization pollination setting rate

The present invention discloses a method for improving the Guangzhou cherry blossom and China Red cherry blossom hybridization pollination setting rate. According to the present invention, different cherry blossom varieties having different flowering phases are adjusted to flower at the same period so as to ensure the freshness of the hybridization pollination pollen, improve the hybridization pollination setting rate, and improve the flexibility of male parent and female parent selection. According to the present invention, after the female parent is subjected to emasculation, the pollination is instantly performed, such that the operation is simple, and the female parent is prevented from damage during the multiple bagging and bag breaking processes; after completing the hybridization pollination, the white transparent slight thin plastic bag is used to sleeve, wherein the cherry blossom branch is slender and weak and easily droops after being sleeved by the kraft bag, and the cherry blossom branch is easily broken in the wind and rain weather so as to cause the pollination failure; and the white plastic bag of the hybridization fruit with the characteristic of primary pollination success is released, and the white nylon mesh bag with the compact meshes is used to sleeve, such that the normal growth development of the fruit is not affected, the bird foraging can be prevented, the united fruit harvest is easily achieved, and the large area hybridization pollination fruit ripening harvest efficiency is substantially improved.
Owner:GUANGZHOU TRENDSEE GRP +3

Photinia disinsectization device for municipal garden

The invention discloses a photinia disinsectization device for a municipal garden. The photinia disinsectization device comprises a box body, wherein a partition plate is arranged on the top internalwall of the box body, a through hole is formed in the external wall of one side of the box body, a bracket is arranged on the bottom internal wall of the box body, a lamp holder is arranged on the topexternal wall of the bracket, a light trap is in threaded connection with the bottom internal wall of the lamp holder, a trapping hopper is in socket joint with the external wall of one side of the partition plate, an insect removing box is arranged on the external wall of one side of the trapping hopper, an insect removing plate is arranged on the external wall of one side of the partition plate, first baffles which are of scattered distribution are separately arranged on the insect removing plate and the external wall of one side of the partition plate, a connecting net is arranged on the external wall of one side of the partition plate, the cross section of the connecting net is W-shaped, a motor is arranged on the external wall of one side of the box body, and a first rotating shaft is fixedly connected with the external wall of one end of an output shaft of the motor. According to the photinia disinsectization device for the municipal garden, mosquitoes and beneficial insects canbe effectively separated, and the influence on pollination work of photinia due to the fact that the beneficial insects are killed by subsequent devices is avoided.
Owner:吴槚婷

Wheat grain lead control agent as well as preparation method and application method thereof

The invention provides a wheat grain lead control agent as well as a preparation method and application method thereof. The wheat grain lead control agent is prepared from an agent A, an agent B and an agent C. The agent A is prepared from 6-7% of calcium phosphate, 3-4% of Fe3O4@C-COOH, 0.1-0.3% of nano manganese sulfate, 0.1-0.3% of nano zinc sulfate, 0.1-0.3% of nano magnesium sulfate and the balance of urea. The agent B is prepared from the following raw materials of: 0.5-1.5% of nano magnesium sulfate, 2-4% of humic acid, 0.5-1% of Fe3O4@C-COOH, 0.5-1.5% of alkyl naphthalene sulfonate and the balance of water. The agent C is prepared from the following raw materials of: 0.2-0.4% of nano manganese sulfate, 0.2-0.4% of nano zinc sulfate, 2-4% of humic acid, 0.1-0.3% of Fe3O4@C-COOH, 0.5-1.5% of alkyl naphthalene sulfonate and the balance of water. The invention also provides an application method of the lead control agent. The method comprises the following steps that: S1: after wheat turns green, the agent A is applied into wheat soil; S2, after the wheat sprouts out stems, the agent B is sprayed to wheat leaves; and S3, after the wheat is under grain filling, the agent C is sprayed onto the wheat leaves. The lead control agent and the application method provided by the invention can be used for atmosphere-soil two-end synergistic lead control on the wheat, and are harmless to the environment and human bodies.
Owner:ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY

Method for efficiently preventing and controlling cadmium pollution in wheat-corn rotation system

The invention relates to the field of environmental governance methods, and particularly discloses a method for efficiently preventing and controlling cadmium pollution in a wheat-corn rotation system. The method mainly comprises the following steps: applying a fertilizer A which is composed of urea, calcium phosphate, Fe3O4@C-NH2, manganese sulfate, zinc sulfate and magnesium sulfate after wheat reviving, applying the fertilizer A in a corn seedling stage after wheat harvesting, spraying a fertilizer B which is prepared from nano magnesium sulfate, humic acid, Fe3O4@C-NH2, alkyl naphthalene sulfonate and water onto leaf surfaces of the wheat and the corn in a wheat and corn elongation stage, and spraying a fertilizer C which is prepared from nano manganese sulfate, nano zinc sulfate, humic acid, Fe3O4@C-NH2, alkyl naphthalene sulfonate and water onto ears of the wheat and the corn after the grain filling stage of the wheat and the grain flowering stage of the corn. According to the method, roots are controlled and prevented from absorbing cadmium in soil in the seedling stage, leaves are controlled and prevented from absorbing cadmium in atmospheric dust fall in the elongation stage, the ears are controlled and prevented from absorbing cadmium in atmospheric dust fall in the grain filling stage of the wheat and the grain flowering stage of the corn, and the purposes of relay prevention and control in the growth stage, crop rotation system prevention and control and prevention and control cost reduction are achieved.
Owner:ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY

Ear retarder for reducing lead in wheat grains as well as preparation and application methods thereof

The invention belongs to the technical field of environmental pollution treatment, and particularly relates to an ear retarder for reducing lead in wheat grains and a preparation and application method thereof. The ear retarder is prepared from the following raw materials in percentage by mass: 0.2 to 0.4 percent of manganese sulfate, 0.2 to 0.4 percent of magnesium sulfate, 0.2 to 0.4 percent of zinc sulfate, 3 percent of humic acid, 0.2 percent of Fe3O4@C-COOH, 1 percent of alkyl naphthalene sulfonate, 0.2 percent of cyhalothrin and the balance of water. The preparation method comprises the following steps: S1, respectively weighing the following raw materials in percentage by mass: 0.2 to 0.4 percent of manganese sulfate, 0.2 to 0.4 percent of magnesium sulfate, 0.2 to 0.4 percent of zinc sulfate, 3 percent of humic acid, 0.2 percent of Fe3O4-coated C-COOH, 1 percent of alkyl naphthalene sulfonate, 0.2 percent of cyhalothrin and the balance of water; and S2, uniformly mixing the raw materials in the S1 to obtain the ear retarder. The ear retarder provided by the invention can achieve the effect of reducing the content of Pb in wheat grains by inhibiting the absorption of Pb in atmospheric dust fall by wheat ears; and according to the ear retarder provided by the invention, all the components are harmless to a human body, the application mode is simple, and the beneficial effects are obvious.
Owner:ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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