Simple and efficient field inoculation method for rice false smut germ

Abstract

The invention belongs to the technical field of plant protection and particularly relates to a simple and efficient field inoculation method for a rice false smut germ. The method comprises the steps: picking rice false smut balls from rice ears in a rice field at a rice ripening period in last year, drying the rice false smut balls in the air indoors, loading the dried rice false smut balls into plastic bags or plastic bottles, and placing the plastic bags or plastic bottles ay an indoor shady place; and at a 3-4 stage of young panicle differentiation of rice, putting the rice false smut balls preserved in last year into clean water, carrying out soaking for 3-5 hours, then, carrying out oscillating so as to enable rice false smut powder on the outside surface of each rice false smut ball to gradually fall off until rice grains are clearly discernible, then, enabling the rice false smut powder to be completely scattered into water, carrying out filtering with gauze so as to filter off grain residues and other foreign bodies, then, pouring filter liquor into a sprayer, adjusting water volume according to a spraying area, directly spraying the filter liquor to fields at 10 am to 5 pm in a sunny day, and swinging the sprayer during spraying so as to achieve uniform spraying as far as possible. By adopting the method disclosed by the invention, the onset ear ratio of field artificial inoculation of rice false smut can reach about 30% and is extremely stable. The method disclosed by the invention is extremely simple and can be used for carrying out field identification on degree of disease resistance to rice false smut of rice varieties without special utensils and conditions.

Description

technical field

[0001] The invention belongs to the technical field of plant protection, and in particular relates to a simple and efficient field inoculation method for rice smut fungus. Background technique

[0002] Rice false smut is a disease of rice ear grains caused by a fungus (Villosiclavavirens (Nakata) E. Tanaka & C. Tanaka). The mycelial mass formed in the injured panicle swells to form a rice ball several times larger than the grain. Rice balls not only affect the yield of rice and the quality of rice, but also contain rice aspergillus toxins that are highly toxic to humans and livestock, have an inhibitory effect on microtubulin in animals, and can cause kidney, liver and bladder cells in mice necrosis. Rice false smut is a worldwide fungal disease, which occurs on a large scale in many countries and regions such as America, Africa, and Asia. When the disease is serious, the incidence rate can be as high as more than 40%. Due to many changes in rice cultivati...