The application discloses a method for promoting
paddy field biological
nitrogen fixation based on long-distance
electron transfer, and comprises the following steps: S1, soil pretreatment, establishing a flooding
system; S2, pre-incubation to form a stable
redox stratification; S3, constructing a long-distance
electron transfer channel; S4, cultivation: under the conditions of flooding and a certain temperature, continue to cultivate to promote the
paddy field biological
nitrogen fixation in the flooding
system. The application does not need an external power supply, only by arranging a
graphite rod in the flooded
paddy field soil, making it span the
surface layer oxidation zone and the lower layer reduction zone, forming a long-distance
electron transfer channel, thereby realizing the directional transport and utilization of the lower layer reduction equivalent to the
surface layer, providing continuous support for the
surface layer nitrogen fixation process from the electron supply end, promoting the
nitrogen fixation process, and also promoting the enrichment of paddy field biological
nitrogen fixation related microbial groups in the surface layer
oxidation zone and changing the iron valence
state distribution, promoting the transformation of Fe(III) into Fe(II), and enhancing the
coupling of
iron reduction and
nitrogen fixation process.