The invention discloses a three-oil one-lycopene soft capsule and a preparation method thereof. The soft capsule is formed by the content and a capsule wall material. The content comprises, by weight, 25-34 parts of perilla oil, 30-36 parts of pumpkin seed oil, 18-26 parts of garlic oil and 10-17 parts of lycopene. The capsule wall material comprises, by weight, 20-35 parts of gelatin, 5-8 parts of glycerinum and 15-35 parts of water. The raw materials used for the soft capsule are all green and healthy foods extracted from pure natural plant seeds and fresh fruits and have no toxic and side effects. The soft capsule has the effects of improving immunity, delaying senescence, preventing cancers, enhancing intelligence, protecting eyesight, resisting irritability, diminishing inflammation, killing bacteria, improving the sleep quality, regulating blood pressure, blood glucose and blood fat and preventing cardiovascular and cerebrovascular diseases; the formula is unique, and the soft capsule is especially suitable for middle-aged and elderly people and sub-health groups; the soft capsule technology is adopted, the dosage form is advanced, and it is guaranteed that the content of effective ingredients is high, the efficacy is lasting, active ingredients can be absorbed easily, the product is not prone to deterioration, and the soft capsule can be stored for 2-4 years at the normal temperature.