89 results about "Panicle" patented technology

The invention belongs to the technical field of agricultural biology, and particularly relates to a plant constitutive promoter OsSULTR2. 2pro and an application thereof. The promoter is characterized in that the promoter is OsSULTR2; the nucleotide sequence of the 2pro is as shown in SEQ ID NO. 1. The promoter provided by the invention is OsSULTR2; 2pro is a rice endogenous constitutive promoter, can drive a target gene to realize efficient and stable expression in plant callus, roots, stems, leaves, young ears, seeds and other tissues in a vegetative growth period, and can replace an existing non-plant-source promoter. The method has important application value in the field of plant genetic engineering, and can effectively reduce potential safety risks of transgenic plants caused by introduction of exogenous DNA.

The invention discloses an effect of OsCPK18 in inhibition of pre-harvest germination of rice. According to the invention, the Zhonghua 11 is taken as the background, and two mutant plants, namely, KO-OsCPK18 # 1 and KO-OsCPK18 # 2, with OsCPK18 gene function defect mutation are obtained through transgenosis; in a seed germination experiment, it is found that seed germination of the OsCPK18 mutant material is delayed, and the final germination rate is not affected; a pre-harvest sprouting simulation experiment is carried out in a high-temperature and high-humidity environment arranged in an incubator, and the pre-harvest sprouting resistance of the OsCPK18 mutant material is found to be remarkably higher than that of a wild type. The results show that the OsCPK18 gene under the background of ZH11 has important significance on crop breeding, the invention provides a method for improving the pre-harvest sprouting resistance of rice, and the cultivation process of a new pre-harvest sprouting resistant rice variety can be accelerated.

The application provides a method for increasing the length of a main panicle of rice, the number of secondary branches of the main panicle, the number of grains on the main panicle of rice and / or improving the yield of rice, which comprises overexpressing a cystathionine beta-lyase gene OsCBL in rice by using a recombinant vector containing a CDS coding sequence of the cystathionine beta-lyase gene OsCBL as shown in SEQ ID NO: 2 to obtain a transgenic plant. The transgenic plant obtained by the method has an increased number of grains per panicle and an increased yield per plant compared with normal plants. The method can be used for treating rice plants to improve the yield of rice and provides a new gene resource and a new idea for breeding rice with increased yield.

The invention discloses a method for cultivating a heat-resistant broad-spectrum durable rice blast-resistant rice breeding material based on polygene polymerization. According to the method, a high-heat-resistance and high-dominance indica-japonica hybrid indica rice restorer line is selected as a recurrent parent, a breeding intermediate material for polymerizing complementary broad-spectrum rice blast resistance genes Pigm and Pi-jx is used as a donor parent, and polymerization of the heat-resistance and broad-spectrum lasting rice blast resistance genes Pigm and Pi-jx is realized through a method of combining molecular breeding with conventional breeding. Meanwhile, by selecting representative strains to carry out artificial inoculation identification of seedling blast and panicle blast, natural induction identification of a rice blast retransmission area and heat resistance identification, and by combining main agronomic trait evaluation in the whole growth period, a heat-resistant broad-spectrum durable rice blast-resistant breeding material is obtained. The breeding material bred by using the method disclosed by the invention has relatively strong heat resistance and broad-spectrum and lasting rice blast resistance, and the breeding process of heat-resistant and rice blast-resistant rice varieties is accelerated.

The invention discloses genetic engineering application of a rice cellulose synthesis gene OsCesA9, belongs to the field of genetic engineering, and discloses cloning of the gene OsCesA9 participating in regulation and control of rice grain length and application of the gene OsCesA9 in regulation and control of rice grain length. The CDS sequence of the gene is as shown in SEQ ID NO.1, and the cDNA sequence is as shown in SEQ ID NO.9. Overexpression of the rice gene OsCesA9 can increase the grain length of rice without influencing the panicle number of the whole plant, and is beneficial to genetic improvement of rice.

A new and distinct hybrid of Heuchera plant named ‘Sultry Night’ has a medium-sized habit with foliage that is moderately-dissected, moderately-wavy, lightly overlapping cordate bases, light marginal undulation, glossy leaf blades of dark purplish-grey with a lighter purplish-red overlay on short petioles. The leaf blades are shallowly dissected at five main lobes with many smaller indentations. The new plant has upright, moderately-dense, branched panicles producing pinkish flower effect. The new plant is useful in the landscape or in containers.

The invention discloses an AP2 gene or an encoding protein thereof and application of the AP2 gene or the encoding protein thereof in increasing plant yield or promoting plant growth. The ZmAP2 gene is identified from corn and is overexpressed in rice, and compared with a control CK, the number of grains per ear of an overexpressed plant is remarkably increased, the grain length is remarkably increased, the thousand grain weight is remarkably increased, the biomass is remarkably increased, and the plant height is remarkably increased; the homologous rice OsAP2 gene is further subjected to overexpression in rice, and compared with a control CK, the overexpressed rice plant grain number per ear is remarkably increased, grains are remarkably lengthened, the thousand grain weight is remarkably increased, and the yield of a single plant is remarkably increased. The invention has application potential in the aspects of promoting crop growth, improving crop yield, cultivating high-yield crop varieties and the like.

A new cultivar of Epimedium plant named ‘Little Bo Peep’ characterized by its compact dense habit and semi-evergreen tri-parted foliage with ovate leaflets that develop as light chartreuse with bronze-burgundy blush in the cooler spring and become medium green in the summer. Medium-sized flowers appear on panicles above the foliage starting in late spring with spurs having a light pinkish-purple tips and medium rose-colored centers, and pinkish-purple sepals. ‘Little Bo Peep’ is useful in the garden landscape as a specimen, en mass, or in containers.

This invention discloses a rice OsRbohB protein, its encoding gene, and its applications. The amino acid sequence of the OsRbohB protein is shown in SEQ ID NO.2, and the nucleotide sequence of its encoding gene is shown in SEQ ID NO.1. This invention provides a rice transformation overexpression vector that overexpresses the OsRbohB gene using the ZmUbi promoter. The plant height, number of grains per panicle, and grain length of the overexpressed plants were significantly increased. This invention is the first to demonstrate that rice OsRbohB is a functional gene regulating rice plant height, number of grains per panicle, and grain length. The cloning and biological function verification of this gene have important reference value for the study of the molecular mechanisms regulating important agronomic traits in rice. Since the above traits are important traits related to rice plant type and yield, the functional analysis of OsRbohB in this invention provides a new candidate gene for rice design breeding and has great potential application value.

Provided are a constitutive promoter and a use thereof. The nucleotide sequence of the constitutive promoter comprises SEQ ID NO:34. The constitutive promoter exhibits activity in almost all tissue and many cell types of plants, particularly in roots, stems, leaves, filaments, pollen, seeds, and the tips of panicle axes, showing broad application prospects in plants.

The new and distinct cultivar of ornamental, winter-hardy, blue fescue plant, Festuca arundinacea ‘Perfect Edging’, with highly vigorous, clumps of tight, full, rounded habit of strong stems and foliage, and mostly upright panicles. The foliage is variegated with creamy margins and dark green centers.

The invention discloses a chenopodium quinoa willd pre-harvest sprouting resistance molecular marker and a screening method and application thereof, and belongs to the technical field of biology, a high-resistance variety QQ65 and a susceptible variety Faro are utilized to construct an F2 segregation population, and through whole genome re-sequencing and map-based cloning, a major QTL for controlling the pre-harvest sprouting resistance of chenopodium quinoa willd is finely positioned in a physical interval of about 60 kb in a chromosome 8. In the interval, low expression of the gene LOC110693583 in the seed germination process of a resistant material is found, high expression of the gene LOC110693583 is induced in a susceptible material, and the sequence variation of the gene LOC110693583 is highly co-separated from the resistance phenotype of the gene LOC110693583. The unique resistance haplotype of the QQ65 in the region is further determined through re-sequencing analysis on a large number of germplasm resources.

The invention discloses an HAPP gene and application thereof, and relates to the field of plant genetic engineering, the identified rice HAPP gene can increase the rice plant height and the seed size at the same time, and after the rice HAPP gene is fused with a PANDA gene, the rice plant height and the seed size can be remarkably increased, but the ear number is reduced. Meanwhile, analysis finds that HAPP originates from a Harbinger transposon Myb-like DNA binding protein, interacts with PANDA and is related to a gene expression mode, but has antagonism in function. Therefore, identification of the HAPP gene not only reveals a new function of the transposon-derived gene in regulation and control of rice yield traits, but also provides new theoretical support and practical guidance for rice breeding.

The invention relates to a plant floral organ dominant expression gene JAZ4 as well as an expression product, an expression vector and application thereof, and aims to solve the technical problem that the development of plant flower color and stigma mastoid cells is difficult to regulate and control at present. A gene JAZ4 capable of regulating and controlling the development of plant floral organs is cloned from tobacco, and by constructing a knockout strain and an overexpression strain of the JAZ4 gene, the flower color becomes shallow after the JAZ4 gene is knocked out, lactiform protrusions of corolla conical epidermis cells become large, the density is obviously increased, and stigma hair is increased; after overexpression, the flower color is deepened, the milky protrusion of the corolla conical epidermis cells almost disappears, the density is obviously reduced, and the stigma hair is deleted, which indicates that the NtJAZ gene can regulate and control the development of the flower color, the corolla conical epidermis cells and the stigma hair; the method is of great significance to research of a molecular regulatory network and molecular assisted breeding of plant flower color and stigma mastoid cell development.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to a rice pre-harvest sprouting resistance character, a detection primer and application of the SNP molecular marker. Analysis is carried out according to germination rate data and genotypes of 249 groups of rice seeds, it is found that difference of SNP with the promoter region position of the LOCOs07g07520 gene being 3744951 can cause significant difference of the germination rate of the group seeds, Hap G has higher germination rate than Hap A, and therefore the SNP molecular marker closely linked with the rice pre-harvest germination resistance character is obtained and located at the 3744951th bp of the No.7 chromosome of the rice, and the SNP molecular marker is located at the 3744951th bp of the No.7 chromosome of the rice. The basic group is A or G; the molecular marker can accurately predict the pre-harvest sprouting resistance of the rice seeds, can be used as an auxiliary selection marker for the pre-harvest sprouting resistance character in the rice breeding process, improves the selection accuracy, accelerates the breeding process, and has application prospects in the aspects of reducing the pre-harvest sprouting rate of the rice seeds or assisting in breeding pre-harvest sprouting resistant rice varieties and the like.

This invention belongs to the field of crop cultivation technology, and in particular relates to a water and fertilizer management method for rice cultivation in saline-alkali land. Combining the technical characteristics of rice cultivation in saline-alkali land, and based on rice leaf age diagnosis technology, it rationally adjusts the nitrogen fertilizer ratio between basal tillers and panicles, and adjusts the application time of tillering fertilizer and panicle fertilizer. In terms of water management, it adopts an alternating shallow, wet, and deep irrigation mode to solve the existing water and fertilizer management technical problems in rice production in saline-alkali land, promote the robustness of individual rice plants, coordinate the contradiction between individual plants and the overall population, improve the overall population quality, and thus achieve high yield and high efficiency in rice cultivation in saline-alkali land.

This invention belongs to the field of agricultural biotechnology, specifically relating to a plant constitutive promoter OsSULTR2;2pro and its applications. The nucleotide sequence of the promoter OsSULTR2;2pro is shown in SEQ ID NO.1. The promoter OsSULTR2;2pro of this invention is an endogenous constitutive promoter for rice, capable of driving efficient and stable expression of target genes in plant callus tissue, roots, stems, leaves during vegetative growth, as well as young panicles and seeds, and can replace existing non-plant-derived promoters. It has significant application value in the field of plant genetic engineering and can effectively reduce the potential safety risks of transgenic plants caused by the introduction of exogenous DNA.

A process for sorting and primary production of C4 plants for subsequent production according to the invention involves management, preparation, and primary production of C4 plants, including an appropriate transportation system, to obtain two or more types of raw materials and products from the process according to the invention. The raw materials and the products obtained will be made to possess suitable and effective properties, as previously specified, prior to the subsequent process, wherein comprising a step of C4 plants through a mechanical method utilising machinery, tools, or equipment designed for harvesting, which separates parts of the C4 plant from the roots soil; and a sorting step, which is to provide for sorting of the parts of the C4 plant from the harvesting step or the preceding step by sorting ears or panicles and stalks from each other, wherein the ears or panicles are subjected to sorting of the C4 plant grains from the ears or panicles, then separating the C4 plant grains from shells / skins by threshing using a machine, tool, or equipment for threshing; and the stalks are subjected to separation of bagasse and sap by a mechanical process; and a primary production step, which aims to facilitate primary production through various methods applied to stalks obtained from the sorting or the preceding step, wherein comprising a primary production process for the grains of the C4 plant, for bagasse, and for the sap or for adjusting its concentration sap, and a primary production step of the stalks or pretreatment of the stalks; and a transportation step, which is to provide the management of a transportation system of the ears or panicles, the grains of the C4 plant, the shells / skins, the stalks, the bagasse, the sap, including the raw materials / products of the C4 plant obtained from the harvesting step, the sorting step, the primary production step, or from any one of the steps appropriately, to ensure optimal efficiency as prescribed for entering the subsequent production.

The invention discloses a related protein for improving the calcium content of rice panicles as well as a coding gene and application of the related protein. The invention provides an application of an OsPAA2 protein or an OsPAA2 gene in regulating and controlling the calcium content of a plant. The invention also provides an application of the OsPAA2 gene or an OsPAA2 gene related biological material in preparation of a plant with increased calcium content. The invention also provides a method for preparing the transgenic plant. The method comprises the following step: introducing the OsPAA2 gene or the OsPAA2 gene related biological material into a receptor plant to obtain the transgenic plant with increased calcium content. The invention also provides an application of the OsPAA2 protein as a plant calcium transporter. The OsPAA2 protein is a protein as shown in SEQ ID NO: 1. And the OsPAA2 gene is a gene for coding an OsPAA2 protein. The method has important theoretical and practical significance for breeding of varieties with high calcium content.

The invention relates to the technical field of agricultural remote sensing and information, and particularly discloses a method and system for simulating a radar backscattering coefficient of a rice field in a heading period, and the method comprises the steps: obtaining geometric and physiological parameters of the rice field, and synchronously and actually measuring radar data; dividing the canopy into a spike leaf mixing layer, a stem leaf mixing layer and an underlying surface layer; five scattering mechanisms of ears, leaves and stems are decomposed; calculating component complex permittivity based on the model; constructing a fine three-dimensional geometric model of the ears, the leaves and the stems; obtaining a scattering matrix of each component through electromagnetic simulation; calculating an interlayer attenuation coefficient and a propagation constant by using the forward scattering matrix; calculating phase deviations under different scattering paths; and finally, performing coherence superposition on the backscattering contributions of the components in combination with phase deviation to obtain a total backscattering electric field and a coefficient. Through combination of component-level fine electromagnetic simulation and a scene-level radiation transfer theory, high-precision and high-efficiency scattering simulation is realized, and remote sensing monitoring of rice is effectively supported.

The invention discloses application of a rice gene OsGN6 to regulation and control of rice grain number per spike and yield. The invention provides application of OsGN6 protein or related biological materials thereof in regulation and control of grain number per ear and yield of plants. According to the invention, an OsGN6-BYP gene is mutated by applying a CRISPR-Cas9 method, and it is found that the OsGN6-BYP gene can significantly reduce the number of grains per ear of rice; and overexpression of the OsGN6-BYP gene can significantly increase the number of grains per ear of rice. Therefore, the OsGN6 gene plays an important role in regulating and controlling the grain number per ear of rice and increasing the yield, and has important significance for cultivating new varieties of high-yield plants.

A rice white leaf and panicle gene wlp3 is provided. The cDNA sequence of the white leaf and panicle gene wlp3 is shown in SEQ ID NO: 1, and the encoded amino acid sequence of the protein is shown in SEQ ID NO: 2. The rice white leaf and panicle gene wlp3 is applied to rice stress resistance and yield increase. The white leaf and panicle gene wlp3 is configured to improve cold tolerance of plants, enhance photosynthetic rate, increase plant height, leaf albinism at seedling stage, panicle albinism at heading stage, and increase panicle length at low temperature. The present disclosure obtains the rice white leaf and panicle gene wlp3 through screening and mutagenesis, which is related to the stress resistance and chlorophyll synthesis of rice. Therefore, the present disclosure provides a foundation for rice breeding.

This invention relates to the field of scientific fertilization and production technology for rice, specifically to a method and application of panicle-stage fertilization to stabilize the number of grains per panicle and improve the panicle type index. The fertilization period is the third leaf from the bottom, which is the stage from the third leaf from the bottom until it is fully expanded. The fertilization amount is 110 kg / ha. ‑1 The fertilizer is urea, and the total nitrogen content of the urea is ≥46%. The nitrogen management method during the heading stage of this invention stabilizes the number of grains per panicle while improving the panicle shape index, thus resolving the synergistic contradiction between the number of grains per panicle and the grain fillability. Compared with the conventional method of applying nitrogen fertilizer during the heading stage at the three-leaf stage, this invention can cultivate a grain-setting characteristic dominated by grains in the middle and upper parts of the panicle, increasing the rice panicle shape index by 8.8%–10.0% and achieving a yield increase of 8.8%–9.1%.

This application belongs to the field of biotechnology and provides a rice transcription factor gene. OsNAC15 Or the application of its encoded protein in regulating rice disease resistance, said rice transcription factor gene OsNAC15 The nucleotide sequence is shown in SEQ ID NO:1; the amino acid sequence of the encoded protein is shown in SEQ ID NO:2. This invention confirms the transgenic function through verification. OsNAC15 It is a positive regulator of rice blast resistance, and overexpression OsNAC15 It can significantly enhance rice's resistance to rice blast (leaf blast), while knocking out this gene leads to a decrease in rice resistance. Moreover, the regulation of this gene does not affect agronomic traits such as plant height, number of panicles, number of grains per panicle, seed setting rate, thousand-grain weight, or rice quality. It provides an ideal gene resource for breeding disease-resistant, high-quality, and high-yield rice varieties.

The invention belongs to the technical field of crossing of agricultural science and crop physiology, and particularly relates to a method for identifying pre-harvest sprouting resistance of quinoa based on physiological characteristics in a grain filling period. According to the invention, systematic research and proving are carried out in quinoa for the first time: systematic and quantifiable differences exist between pre-harvest sprouting resistance varieties and high-susceptibility varieties in carbon metabolism key node enzyme activity and substrate accumulation dynamic states in the middle and later periods of quinoa grain development (namely generalized grouting or substance enrichment stages). The invention further provides a method for identifying the pre-harvest sprouting resistance of the quinoa based on the physiological characteristics of the grain filling stage, and the pre-harvest sprouting resistance of the quinoa can be simply, conveniently and quantitatively identified reliably in the early stage by sampling and measuring the key enzyme activity and the starch conversion strength in the middle and later periods of the development of the quinoa grains.

A new and distinct cultivar of Hydrangea plant named ‘SMNHPLK’ is disclosed, characterized by exceptionally dense mophead panicles with a distinct spherical shape. Sepals open light emerald green, then mature to white and red-pink and aging to entirely red-pink. Stems are strong and thick, supporting large flower panicles without flopping. Plants are compact and upright with heavy flower coverage. The new variety is a Hydrangea, typically produced as an outdoor ornamental plant.

A new and distinct ornamental Big Bluestem Grass Andropogon plant named ‘Blue Steel’ with broad, distally-arching, bluish-green foliage that develops reddish-purple leaf ends about late summer. The upright and dense culms that resist lodging produce medium-height plants with panicles beginning purplish-grey and becoming tan seed heads that are retained into winter. The new plant is useful in the landscape as a specimen or en masse.

This invention discloses a method for increasing the yield of hybrid japonica rice seed production. Belonging to the field of rice seed production technology, the specific steps are as follows: conducting germination tests on the male-sterile and restorer lines of hybrid japonica rice; confirming the sowing time; soaking, germinating, and sowing the seeds; transplanting to a multi-span greenhouse; spraying water and applying temperature stimulation when the male-sterile line flowers, and closing the top film-rolling device; spraying water and applying temperature stimulation when both the male-sterile and restorer lines flower, ensuring complete coverage; after the panicles are sun-dried, they flower again, and the top film-rolling device is opened to facilitate pollination. This invention precisely regulates the flowering time of hybrid japonica rice, promoting earlier flowering of the male-sterile and restorer lines, allowing them to flower simultaneously. Both the glenching angle of the male-sterile line and the pollen quantity of the restorer line are highly concentrated, improving pollination efficiency, increasing the cross-pollination rate of the male-sterile line and the seed production yield, and providing stable working hours, greatly improving individual work efficiency.

The invention relates to the technical field of rice planting, in particular to application of demethylated sprouting lactone in promoting heading and flowering of rice in soda saline-alkali soil and increasing yield, and relates to the technical field of rice planting, in particular to application of demethylated sprouting lactone in promoting heading and flowering of rice in soda saline-alkali soil and increasing yield. The invention aims to solve the problems of thousand seed weight reduction, yield reduction and quality reduction caused by soil salinization, and provides application of demethylated sprouting lactone in promoting heading and flowering of rice in soda saline-alkali soil and increasing yield. By soaking the seeds and spraying a demethylated germinating lactone dimethyl sulfoxide solution with the concentration of 0.1-10 mu mol / L in the tillering stage, heading and flowering of the rice in the soda saline-alkali soil are promoted, and the yield is increased. The method can be suitable for large-area saline-alkali soil rice production, and can effectively improve the effective ear number of a single rice plant, the number of grains per ear, the maturing rate, the thousand seed weight and other yield constituent factors, so that the yield level of saline-alkali soil rice is remarkably improved.

The invention belongs to the technical field of crop heavy metal accumulation phenotype identification and crop breeding, and particularly relates to a method for identifying the low cadmium accumulation characteristic of a rice sterile line and application of the method. According to the method disclosed by the invention, simplification, standardization and high efficiency of cadmium low accumulation characteristic phenotype identification of the sterile line are realized through a standardized identification environment and diversified detection samples which are easy to obtain, such as a small amount of self-fertilized or outfertilized rice and unfertilized empty grains or rice ears or leaves; important support is provided for cultivation and official certification of the low-cadmium sterile line, so that breeding and large-area popularization and application of low-cadmium hybrid rice varieties are improved.