144 results about "Panicle" patented technology

The invention belongs to the technical field of plant gene engineering, and particularly relates to an OsNUC1 gene and application of a gene mutant constructed by the OsNUC1 gene to regulation and control of rice plant height, leaf length and panicle type. The regulation is to regulate the plant height and leaf growth of the rice and influence the shape of the ear part. Compared with the prior art, the OsNUC1 gene mutant has the following advantages: (1) the OsNUC1 is directionally edited by utilizing a CRISPR / Cas9 gene editing technology, an OsNUC1 gene mutant rice material is obtained, the biological phenotype of the OsNUC1 gene mutant rice material is investigated, the phenotype investigation on T1-generation seedlings of the OsNUC1 shows that the plant heights and the leaf lengths of one-week-old and two-week-old seedlings of the mutant are obviously higher than those of wild seedlings, the leaf widths have no obvious difference, and the OsNUC1 gene mutant rice material has a good application prospect; the result shows that the OsNUC1 can regulate the plant height and the leaf growth of the rice and influence the shape of the ear part. (2) it is proved that the nucleolin gene OsNUC1 is negatively regulated by a transcription factor OsARF7, and the phenotypes such as the plant height, the leaf length, the grain length and the grain width of the OsNUC1 / OsARF7 mutant are more obvious than those of the OsNUC1 mutant. The invention further proves the application of the OsNUC1 gene in regulation and control of rice plant height and leaf growth.

The invention relates to the technical field of plant genetic breeding, in particular to a rice sheath blight resistance related gene and an encoding protein and application thereof. The invention provides a rice sheath blight resistance related gene as well as an encoding protein and application thereof, the gene is OsSBR4 or a related gene thereof, the resistance of rice to sheath blight can be remarkably enhanced by knocking out the gene, and the resistance of rice to sheath blight can be reduced by overexpressing the gene. After agronomic traits of a transgenic line of the gene are investigated, it is found that the plant height, the ear length, the ear number, the thousand seed weight and the like are not affected, and it is indicated that the gene has important application value in sheath blight resistance molecular breeding. According to the invention, a novel rice sheath blight resistance related gene is screened and excavated, and the identification of the sheath blight resistance gene can provide a theory and a basis for breeding a sheath blight resistance rice variety.

The invention discloses a cone inflorescence type fruit tree flower three-dimensional reconstruction male and female flower counting method and system, and the method comprises the steps: S1, collecting an image sequence, shooting an inflorescence video through a camera, and extracting the video into an image frame by frame; s2, image preprocessing and camera parameter estimation: preprocessing the inflorescence image, and acquiring a camera parameter corresponding to each image by using a COLMAP tool; s3, a D-NeRF model is trained, and multi-modal three-dimensional reconstruction of the inflorescence point cloud is carried out by using the D-NeRF model; s4, the three-dimensional point cloud output by the D-NeRF model is optimized and marked; s5, training a PointNet + + model, and performing male and female flower identification and counting by using the PointNet + + model; and S6, dividing a data set, and adopting a Bayesian optimization algorithm to adjust and optimize key hyper-parameters in the training process of the D-NeRF model and the PointNet + + model. According to the invention, through integration of NeRF reconstruction, point cloud processing, deep learning target detection and mobile terminal deployment, three-dimensional reconstruction of fruit tree inflorescence and male and female flower identification and counting are realized.

The invention belongs to the technical field of agricultural biology, and particularly relates to a plant constitutive promoter OsSULTR2. 2pro and an application thereof. The promoter is characterized in that the promoter is OsSULTR2; the nucleotide sequence of the 2pro is as shown in SEQ ID NO. 1. The promoter provided by the invention is OsSULTR2; 2pro is a rice endogenous constitutive promoter, can drive a target gene to realize efficient and stable expression in plant callus, roots, stems, leaves, young ears, seeds and other tissues in a vegetative growth period, and can replace an existing non-plant-source promoter. The method has important application value in the field of plant genetic engineering, and can effectively reduce potential safety risks of transgenic plants caused by introduction of exogenous DNA.

The invention discloses an effect of OsCPK18 in inhibition of pre-harvest germination of rice. According to the invention, the Zhonghua 11 is taken as the background, and two mutant plants, namely, KO-OsCPK18 # 1 and KO-OsCPK18 # 2, with OsCPK18 gene function defect mutation are obtained through transgenosis; in a seed germination experiment, it is found that seed germination of the OsCPK18 mutant material is delayed, and the final germination rate is not affected; a pre-harvest sprouting simulation experiment is carried out in a high-temperature and high-humidity environment arranged in an incubator, and the pre-harvest sprouting resistance of the OsCPK18 mutant material is found to be remarkably higher than that of a wild type. The results show that the OsCPK18 gene under the background of ZH11 has important significance on crop breeding, the invention provides a method for improving the pre-harvest sprouting resistance of rice, and the cultivation process of a new pre-harvest sprouting resistant rice variety can be accelerated.

The application provides a method for increasing the length of a main panicle of rice, the number of secondary branches of the main panicle, the number of grains on the main panicle of rice and / or improving the yield of rice, which comprises overexpressing a cystathionine beta-lyase gene OsCBL in rice by using a recombinant vector containing a CDS coding sequence of the cystathionine beta-lyase gene OsCBL as shown in SEQ ID NO: 2 to obtain a transgenic plant. The transgenic plant obtained by the method has an increased number of grains per panicle and an increased yield per plant compared with normal plants. The method can be used for treating rice plants to improve the yield of rice and provides a new gene resource and a new idea for breeding rice with increased yield.

The invention discloses a method for cultivating a heat-resistant broad-spectrum durable rice blast-resistant rice breeding material based on polygene polymerization. According to the method, a high-heat-resistance and high-dominance indica-japonica hybrid indica rice restorer line is selected as a recurrent parent, a breeding intermediate material for polymerizing complementary broad-spectrum rice blast resistance genes Pigm and Pi-jx is used as a donor parent, and polymerization of the heat-resistance and broad-spectrum lasting rice blast resistance genes Pigm and Pi-jx is realized through a method of combining molecular breeding with conventional breeding. Meanwhile, by selecting representative strains to carry out artificial inoculation identification of seedling blast and panicle blast, natural induction identification of a rice blast retransmission area and heat resistance identification, and by combining main agronomic trait evaluation in the whole growth period, a heat-resistant broad-spectrum durable rice blast-resistant breeding material is obtained. The breeding material bred by using the method disclosed by the invention has relatively strong heat resistance and broad-spectrum and lasting rice blast resistance, and the breeding process of heat-resistant and rice blast-resistant rice varieties is accelerated.

The invention discloses a method for carrying out water-saving irrigation according to temperature difference inside and outside a rice canopy, which is characterized in that the temperature difference inside and outside the rice canopy is used as a diagnostic index of irrigation, and an irrigation water layer in a field is determined according to growth periods and soil types. Compared with a conventional irrigation method, the method for carrying out water-saving irrigation according to the difference value of the internal temperature and the external temperature of the rice canopy has the advantages that the number of grains per ear, the maturing rate and the thousand seed weight are remarkably increased, so that the yield is remarkably increased, and the amplification is 9.65%-14.46%. Compared with a conventional irrigation method, the method for carrying out water-saving irrigation according to the difference value of the internal temperature and the external temperature of the rice canopy can reduce the irrigation water consumption by 24.33%-26.38%, and the utilization efficiency of the irrigation water can be improved by 46.71%-53.23%.

The invention provides a leaf image mode classification method and system for double cropping rice spike fertilization, and the method comprises the steps: collecting a first leaf image parameter array and a second leaf image parameter array at a preset time window of target double cropping rice and at a plurality of positions of a first leaf and a second leaf; carrying out image parameter discrete analysis on the parameter arrays to obtain a first dispersion and a second dispersion, and calculating to obtain a leaf pollution degree by combining a preset dispersion; and carrying out averaging processing and leaf pattern classification on the parameter array, and respectively obtaining two leaf pattern arrays based on the image parameters and the pollution degree, thereby determining a proper leaf pattern and carrying out double cropping rice panicle fertilizer application. The technical problem that in the prior art, due to the fact that the test data amount is insufficient, the fertilization recommendation accuracy on different field parcels is not high is solved, and the technical effects that through analysis of the double cropping rice leaf image parameters and the mode classification of the pollution degree, accurate regulation and control of the panicle fertilizer are achieved, and the fertilization recommendation accuracy is improved are achieved.

The invention discloses genetic engineering application of a rice cellulose synthesis gene OsCesA9, belongs to the field of genetic engineering, and discloses cloning of the gene OsCesA9 participating in regulation and control of rice grain length and application of the gene OsCesA9 in regulation and control of rice grain length. The CDS sequence of the gene is as shown in SEQ ID NO.1, and the cDNA sequence is as shown in SEQ ID NO.9. Overexpression of the rice gene OsCesA9 can increase the grain length of rice without influencing the panicle number of the whole plant, and is beneficial to genetic improvement of rice.

A new and distinct hybrid of Heuchera plant named ‘Sultry Night’ has a medium-sized habit with foliage that is moderately-dissected, moderately-wavy, lightly overlapping cordate bases, light marginal undulation, glossy leaf blades of dark purplish-grey with a lighter purplish-red overlay on short petioles. The leaf blades are shallowly dissected at five main lobes with many smaller indentations. The new plant has upright, moderately-dense, branched panicles producing pinkish flower effect. The new plant is useful in the landscape or in containers.

The invention discloses a rice cultivation method capable of effectively reducing cadmium content of rice, and relates to the technical field of rice cultivation methods, the rice cultivation method comprises the following raw materials in parts by weight: a base fertilizer, a cadmium reducing agent, a biochar-loaded microbial agent, a nano-hydroxyapatite suspension, a humic acid-silicon material, magnetic biochar and a duckweed seed source, and comprises the following steps: in a germination period, carrying out germination; detecting the cadmium content of a rice seed culture solution, adding a charcoal-loaded microbial inoculum and a nano-hydroxyapatite suspension to adjust the pH value, spreading rice seeds into the culture solution, and soaking; in the seedling stage, a humic acid-silicon material and magnetic biochar are spread; in the period of seedling establishment, duckweed seed sources are sown, base fertilizer is applied after irrigation, seedlings are transplanted, and fishes are put synchronously; in the tillering stage, the cadmium content of the duckweed is monitored, and nano silica sol is sprayed; removing duckweed before heading; in the young ear stage, the cadmium reducing agent is added twice, and by means of pollution removal, physiological barrier and resource circulation, absorption and transfer of cadmium are reduced, bioavailability of cadmium is reduced, and the cadmium content of rice is effectively reduced.

The invention discloses an AP2 gene or an encoding protein thereof and application of the AP2 gene or the encoding protein thereof in increasing plant yield or promoting plant growth. The ZmAP2 gene is identified from corn and is overexpressed in rice, and compared with a control CK, the number of grains per ear of an overexpressed plant is remarkably increased, the grain length is remarkably increased, the thousand grain weight is remarkably increased, the biomass is remarkably increased, and the plant height is remarkably increased; the homologous rice OsAP2 gene is further subjected to overexpression in rice, and compared with a control CK, the overexpressed rice plant grain number per ear is remarkably increased, grains are remarkably lengthened, the thousand grain weight is remarkably increased, and the yield of a single plant is remarkably increased. The invention has application potential in the aspects of promoting crop growth, improving crop yield, cultivating high-yield crop varieties and the like.

A new cultivar of Hydrangea arborescens plant named ‘GRHYAR2008’ that is characterized by its inflorescences with flowers that are deep purple in color that change to red-purple in color and finally light purple-pink in color, its medium sized and compact plant habit, its large flower panicles, and its sturdy stems.

A new cultivar of Epimedium plant named ‘Little Bo Peep’ characterized by its compact dense habit and semi-evergreen tri-parted foliage with ovate leaflets that develop as light chartreuse with bronze-burgundy blush in the cooler spring and become medium green in the summer. Medium-sized flowers appear on panicles above the foliage starting in late spring with spurs having a light pinkish-purple tips and medium rose-colored centers, and pinkish-purple sepals. ‘Little Bo Peep’ is useful in the garden landscape as a specimen, en mass, or in containers.

The invention discloses an APO1 promoter cis-acting element for promoting increase of primary branches of rice panicles and application of the APO1 promoter cis-acting element, and belongs to the technical field of plant genetic engineering. According to the invention, a cis-acting element in the APO1 promoter is excavated, and a CRISPER / Cas9 technology is utilized to prove that the sequence deletion of the cis-acting element can realize moderate increase of primary branches of rice panicles so as to regulate and control panicle types, so that the cis-acting element can be used for rapidly improving the panicle types and the grain number per panicle of rice varieties.

The invention relates to the technical field of molecular breeding, in particular to a novel rice Gn1a allele Gn1a G556D and application of the novel rice Gn1a allele Gn1a G556D in breeding. The invention provides a new allele Gn1a G556D of the rice Gn1a gene and a cytokinin oxidase / dehydrogenase OsCKX2 mutant encoded by the same, and the allele can improve the panicle type structure and increase the yield, can be used for improving germplasm resources of plants and cultivating new varieties of high-yield plants, and has important application value in the field of plant breeding.

This invention discloses a rice OsRbohB protein, its encoding gene, and its applications. The amino acid sequence of the OsRbohB protein is shown in SEQ ID NO.2, and the nucleotide sequence of its encoding gene is shown in SEQ ID NO.1. This invention provides a rice transformation overexpression vector that overexpresses the OsRbohB gene using the ZmUbi promoter. The plant height, number of grains per panicle, and grain length of the overexpressed plants were significantly increased. This invention is the first to demonstrate that rice OsRbohB is a functional gene regulating rice plant height, number of grains per panicle, and grain length. The cloning and biological function verification of this gene have important reference value for the study of the molecular mechanisms regulating important agronomic traits in rice. Since the above traits are important traits related to rice plant type and yield, the functional analysis of OsRbohB in this invention provides a new candidate gene for rice design breeding and has great potential application value.

Provided are a constitutive promoter and a use thereof. The nucleotide sequence of the constitutive promoter comprises SEQ ID NO:34. The constitutive promoter exhibits activity in almost all tissue and many cell types of plants, particularly in roots, stems, leaves, filaments, pollen, seeds, and the tips of panicle axes, showing broad application prospects in plants.

The invention relates to DNA (deoxyribonucleic acid) of a nitrogen efficient gene OsbZip45 for regulating and controlling the plant height, the effective ear number and the yield of rice and breeding utilization of protein of the nitrogen efficient gene OsbZip45. A transcription factor OsbZip45 is identified through an OsCKX2 promoter combined element prediction and upstream transcription factor screening technology, and an OsbZip45-OsCKX2 regulation module is identified through a molecular technology. Through a gene editing technology, a knockout and overexpression material of the OsbZip45 gene is obtained. By knocking out OsbZip45, the plant height, the number of effective panicles and the yield of rice are improved, and the characteristics of green nitrogen and high efficiency are shown; overexpression of the OsbZip45 reduces the plant height, the number of effective panicles and the yield of the rice and reduces the utilization efficiency of nitrogen. The OsbZip45 gene editing primer is developed and is used for nitrogen-efficient breeding of rice. The OsbZip45 gene editing material provides gene resources, materials and technical supports for cultivating green nitrogen efficient rice varieties.

The invention relates to the technical field of agricultural planting, in particular to a high-yield hybrid rice simplified cultivation method which comprises the following steps: S1, sowing in good time to avoid the influence of bad weather; s2, the number of hybrid rice seeds and basic seedlings is increased, and effective panicles are greatly increased; s3, a high fertilizer application level is kept, and scientific fertilizer and water management is conducted; s4, final-period management is well grasped; s5, implementing a lodging-resistant technology; s6, preventing and treating diseases, pests and weeds. According to the light and simple cultivation method for the high-yield hybrid rice, through basic seedling increasing, scientific fertilizer and water management and lodging-resistant and disease and pest prevention technologies, effective panicles are greatly increased, the total grain number is properly reduced, the maturing rate is increased, a high-yield population structure suitable for modern agricultural light and simple cultivation is formed, and the yield of the high-yield hybrid rice is increased by applying measures suitable for large-area light and simple cultivation. The effective panicle and the maturing rate of the high-yield hybrid rice variety are greatly increased, and the purposes of adapting to modern agricultural production and development requirements and improving the large-area rice yield level are achieved.

The new and distinct cultivar of ornamental, winter-hardy, blue fescue plant, Festuca arundinacea ‘Perfect Edging’, with highly vigorous, clumps of tight, full, rounded habit of strong stems and foliage, and mostly upright panicles. The foliage is variegated with creamy margins and dark green centers.

The invention discloses a chenopodium quinoa willd pre-harvest sprouting resistance molecular marker and a screening method and application thereof, and belongs to the technical field of biology, a high-resistance variety QQ65 and a susceptible variety Faro are utilized to construct an F2 segregation population, and through whole genome re-sequencing and map-based cloning, a major QTL for controlling the pre-harvest sprouting resistance of chenopodium quinoa willd is finely positioned in a physical interval of about 60 kb in a chromosome 8. In the interval, low expression of the gene LOC110693583 in the seed germination process of a resistant material is found, high expression of the gene LOC110693583 is induced in a susceptible material, and the sequence variation of the gene LOC110693583 is highly co-separated from the resistance phenotype of the gene LOC110693583. The unique resistance haplotype of the QQ65 in the region is further determined through re-sequencing analysis on a large number of germplasm resources.

A new and distinct cultivar of Miscanthus plant named ‘Lady in Red’ is disclosed, characterized by spectacular red foliage in autumn. Red autumn leaves contrast beautifully with silvery-white flower panicles. Plants are low-maintenance and winter-hardy. The new variety is a Miscanthus, typically produced as an outdoor ornamental plant.

The invention belongs to the field of molecular biology, and particularly relates to application of OsPLDRP1 protein or a coding gene thereof to improvement of rice broad-spectrum disease resistance and regulation of agronomic traits. According to the application, an OsPLDRP1 knockout mutant and an overexpression transgenic line are constructed under the background of susceptible rice, resistance phenotype analysis is carried out by inoculating various pathogenic bacteria (including pyricularia oryzae, rhizoctonia solani and xanthomonas oryzae), and it is proved that OsPLDRP1 participates in regulation and control of immunoreaction pathways of rice to fungal diseases (rice blast and banded sclerotial blight) and bacterial diseases (xanthomonas oryzae). Meanwhile, it is found that the ear length of a mutant material of the OsPLDRP1 is shorter than that of a wild type, after driving is conducted through a strong promoter ubi, the plant type of an overexpression material of the OsPLDRP1 is looser than that of the wild type, the plant becomes higher, the heading phenotype is early, and the OsPLDRP1 influences normal growth and development of rice while stimulating plant immune response. The invention provides important gene resources and molecular theoretical basis for developing a new variety of broad-spectrum disease-resistant rice.

The invention discloses an HAPP gene and application thereof, and relates to the field of plant genetic engineering, the identified rice HAPP gene can increase the rice plant height and the seed size at the same time, and after the rice HAPP gene is fused with a PANDA gene, the rice plant height and the seed size can be remarkably increased, but the ear number is reduced. Meanwhile, analysis finds that HAPP originates from a Harbinger transposon Myb-like DNA binding protein, interacts with PANDA and is related to a gene expression mode, but has antagonism in function. Therefore, identification of the HAPP gene not only reveals a new function of the transposon-derived gene in regulation and control of rice yield traits, but also provides new theoretical support and practical guidance for rice breeding.

The application relates to the technical field of plant molecular biology, in particular to a ZmHPE3 gene based on multi-omics intelligent design and application thereof. The CDS sequence of the ZmHPE3 gene is shown as SEQ ID No. 2, and the amino acid sequence of the encoded protein is shown as SEQ ID No. 1. After overexpression of the gene ZmHPE3 in crops such as rice, the plant photosynthetic efficiency and the number of grains per panicle can be increased, the plant height can be moderately reduced, and the yield can be significantly increased, so that gene resources and material resources are provided for creating crops with high light efficiency and high yield.

The invention relates to a plant floral organ dominant expression gene JAZ4 as well as an expression product, an expression vector and application thereof, and aims to solve the technical problem that the development of plant flower color and stigma mastoid cells is difficult to regulate and control at present. A gene JAZ4 capable of regulating and controlling the development of plant floral organs is cloned from tobacco, and by constructing a knockout strain and an overexpression strain of the JAZ4 gene, the flower color becomes shallow after the JAZ4 gene is knocked out, lactiform protrusions of corolla conical epidermis cells become large, the density is obviously increased, and stigma hair is increased; after overexpression, the flower color is deepened, the milky protrusion of the corolla conical epidermis cells almost disappears, the density is obviously reduced, and the stigma hair is deleted, which indicates that the NtJAZ gene can regulate and control the development of the flower color, the corolla conical epidermis cells and the stigma hair; the method is of great significance to research of a molecular regulatory network and molecular assisted breeding of plant flower color and stigma mastoid cell development.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to a rice pre-harvest sprouting resistance character, a detection primer and application of the SNP molecular marker. Analysis is carried out according to germination rate data and genotypes of 249 groups of rice seeds, it is found that difference of SNP with the promoter region position of the LOCOs07g07520 gene being 3744951 can cause significant difference of the germination rate of the group seeds, Hap G has higher germination rate than Hap A, and therefore the SNP molecular marker closely linked with the rice pre-harvest germination resistance character is obtained and located at the 3744951th bp of the No.7 chromosome of the rice, and the SNP molecular marker is located at the 3744951th bp of the No.7 chromosome of the rice. The basic group is A or G; the molecular marker can accurately predict the pre-harvest sprouting resistance of the rice seeds, can be used as an auxiliary selection marker for the pre-harvest sprouting resistance character in the rice breeding process, improves the selection accuracy, accelerates the breeding process, and has application prospects in the aspects of reducing the pre-harvest sprouting rate of the rice seeds or assisting in breeding pre-harvest sprouting resistant rice varieties and the like.

This invention belongs to the field of crop cultivation technology, and in particular relates to a water and fertilizer management method for rice cultivation in saline-alkali land. Combining the technical characteristics of rice cultivation in saline-alkali land, and based on rice leaf age diagnosis technology, it rationally adjusts the nitrogen fertilizer ratio between basal tillers and panicles, and adjusts the application time of tillering fertilizer and panicle fertilizer. In terms of water management, it adopts an alternating shallow, wet, and deep irrigation mode to solve the existing water and fertilizer management technical problems in rice production in saline-alkali land, promote the robustness of individual rice plants, coordinate the contradiction between individual plants and the overall population, improve the overall population quality, and thus achieve high yield and high efficiency in rice cultivation in saline-alkali land.