59 results about "Nucleolin" patented technology

The present invention provides for diagnostic kits for identifying cancer patients who are more susceptible to cancer therapies employing endostatin and other angiogenesis inhibitors, based upon the discovery that Nucleolin is a specific receptor for Endostatin. In particular, the diagnostic kits include antibody molecules against Nucleolin, DNA or RNA molecules that specifically bind to nucleic acid molecules encoding Nucleolin. The present invention also discloses methods of screening for angiogenesis inhibitors which specifically interact with Nucleolin, and act as angiogenesis inhibitors in an analogous manner as Endostatin. In addition, the present invention discloses methods of inhibiting the proliferation of endothelial cells or angiogenesis of tumor by administering an anti-nucleolin antibody linked to a cytotoxic agent such as tumor necrosis factor alpha to the endothelial cells.

The invention discloses an environmental pH stimuli-responsive type tumor targeting and controlled drug release nano-carrier and a preparation method of the nano-carrier. Dendrimer macromolecular nano-polymer material PAMAM (polyamidoamine) is taken as a main body of a structure and connected with an amphiphilic block copolymer PEG (methoxy polyethylene glycol)-PLA (poly-l-aspartic acid), DOX (doxorubicin) is covalently conjugated to a hydrophobic fragment, namely, PLA, of an arm of the amphiphilic block copolymer and is connected though a pH sensitive hydrazone bond, so that a system has thecharacteristic of in-vivo smart drug release, and drug release can be controlled by in-vivo pH. The connection with a F3 polypeptide nucleolin targeting ligand is realized through a hydrophilic PEG chain segment, and tumor targeting of the system is realized in specific uptake of tumor and nuclear localization. The nano-carrier has potential clinical application value, and powerful technical support is provided for treatment of other types of malignant tumor cells.

Compositions and methods for modulating tumor proliferation in an individual are provided. The methods employ nucleolin-binding agents, such as aptamers. The aptamers of the present invention can be used to modulate the proliferation of malignant, dysplastic; hyperproliferative, and / or metastatic cells through interference with molecular interactions and functions of nucleolin in the tumor cell.

The present invention provides a method for inhibiting the proliferation of malignant and / or hyperplastic cells in a subject by administering to the subject a therapeutically effective amount of a guanosine rich oligonucleotide. The present invention also provides oligonucleotides which are capable of being specifically bound to a specific cellular protein which is nucleolin and / or nucleolin in nature, which is implicated in the proliferation of cells, specifically malignant and / or hyperplastic cells, and a method for their selection.

The invention relates to a copper phthalocyanine molecule-based detection, imaging and treatment integrated nanoprobe. The copper phthalocyanine molecule-based detection, imaging and treatment integrated nanoprobe comprises hexagonal boron nitride nanosheets, copper phthalocyanine molecules and two groups of oligonucleotide binding sequences. One end of each of the two groups of oligonucleotide sequences designed by the invention is a base pair for detecting and circularly amplifying microRNA-21, and the other end is an AS1411 sequence. The AS1411 sequence not only can recognize nucleolin highly expressed on the surfaces of tumor cells to play a role in targeted localization, but also can carry copper phthalocyanine molecules with poor water solubility based on a G quadruplex formed underspecific conditions, then, the nucleotide sequence loaded with copper phthalocyanine is adsorbed on the surfaces of the hexagonal boron nitride nanosheets through pi-pi accumulation to enter tumor cells. The invention further discloses application of the copper phthalocyanine molecule-based detection, imaging and treatment integrated nanoprobe in diagnosis and treatment of tumor cells and living bodies. The diagnosis and treatment integrated nanoprobe can perform in-situ Raman diagnosis on tumor cells, is high in sensitivity and specificity, can realize photodynamic therapy at the same time, and has a certain application value in biological medicine and clinical diagnosis and treatment.

Compositions and methods for modulating tumor proliferation in an individual are provided. The methods employ nucleolin-binding agents, such as aptamers. The aptamers of the present invention can be used to modulate the proliferation of malignant, dysplastic, hyperproliferative, and / or metastatic cells through interference with molecular interactions and functions of nucleolin in the tumor cell.

The present invention provides for diagnostic kits for identifying cancer patients who are more susceptible to cancer therapies employing endostatin and other angiogenesis inhibitors, based upon the discovery that Nucleolin is a specific receptor for Endostatin. In particular, the diagnostic kits include antibody molecules against Nucleolin, DNA or RNA molecules that specifically bind to nucleic acid molecules encoding Nucleolin. The present invention also discloses methods of screening for angiogenesis inhibitors which specifically interact with Nucleolin, and act as angiogenesis inhibitors in an analogous manner as Endostatin. In addition, the present invention discloses methods of inhibiting the proliferation of endothelial cells or angiogenesis of tumor by administering an anti-nucleolin antibody linked to a cytotoxic agent such as tumor necrosis factor alpha to the endothelial cells.

Compositions and methods for modulating tumor proliferation in an individual are provided. The methods employ nucleolin-binding agents, such as aptamers. The aptamers of the present invention can be used to modulate the proliferation of malignant, dysplastic, hyperproliferative, and / or metastatic cells through interference with molecular interactions and functions of nucleolin in the tumor cell.

The invention relates to a targeted antitumor complex based on ricin, a preparing method thereof and applications of the complex. The complex is prepared by compositing carbon quantum dots and a ricinA chain to form a carbon quantum dot-ricin A chain complex, then encapsulating the complex with the lipidosome, and modifying the lipidosome with a nucleolin nucleic acid aptamer. The prepared complex can enter cells more easily, acts on ribosome through a Golgi apparatus path, has higher cytotoxicity, can effectively protect the A chain from being enzymatically decomposed by lysosome, utilizes specificity of the nucleolin nucleic acid aptamer, can be targeted on a tumor cell, reduces toxic and side effects on normal cells, and is of great importance for targeted treatment on tumor cells.

Compositions and methods for modulating tumor proliferation in an individual are provided. The methods employ nucleolin-binding agents, such as aptamers. The aptamers of the present invention can be used to modulate the proliferation of malignant, dysplastic, hyperproliferative, and / or metastatic cells through interference with molecular interactions and functions of nucleolin in the tumor cell.

The invention relates to the technical field of plant genetic engineering, and particularly discloses application of a ZmNuC gene and an encoded protein thereof in resistance to the maize rough dwarf disease. The invention finds for the first time that the expression level of the corn nucleolin gene ZmNuC is in negative correlation with the resistance of corns to the rough dwarf disease; a new way for exploring and creating a new material for resisting to the corn rough dwarf disease is provided; a genetic material foundation is laid for subsequent research; and a good information platform is provided for the resource reservation of the corn rough dwarf disease resistant gene. The research on the rough dwarf disease resistance genetic mechanism and the disease-resistant molecular breeding of plants is promoted; and reliable material and data support is provided for the research on the molecular biology mechanism for enhancing the rough dwarf disease resistance of the plants such as corns and the like.

Improved G-rich oligonucleotide (GRO) aptamers specific to nucleolin, a method of preparing the aptamers, and a use of the aptamers for diagnosing and / or treating a nucleolin-associated disease, are provided.

The invention relates to a nano probe used for nucleolin cross-linking to induce the tumor cell apoptosis, and a preparation method and application of the nano probe, and belongs to the technical field of biology. The nano probe uses ethylene glycol chitosan as a polymer backbone, the polymer backbone is grafted with cholesterol and an A chain, the 5'end of the A chain is modified with a carboxylgroup, the 3'end of the A chain is modified with a dye Cy3, the A chain contains a nucleic acid adapter AS1411 gene sequence, and the A chain partially hybridizes with a B chain modified with BHQ2 atthe 5'end. The nano probe can selectively kill tumor cells with the overexpression of nucleolin, but has relatively little effects on normal cells, is expected to become a method for the treatment ofmalignant tumors, and provides a new alternative receptor for the idea of receptor cross-linking to induce apoptosis. The nano probe is simple in synthesis, separation and purification, and is suitable for expanded production.