8 results about "Biogenesis" patented technology

The invention belongs to the field of biological medicine, and relates to application of triptolide in preparation of a medicine for preventing and treating calcified aortic valve diseases. The triptolide is found to be capable of promoting expression and maturation of CTSD and activating enzyme activity of the CTSD, meanwhile, biogenesis of lysosome in valvular interstitial cells can be promoted by regulating and controlling AKT / mTOR / TFEB signal axes, lysosome functions are improved, autophagy flux is recovered, and finally the effect of treating the calcified aortic valve disease is achieved. A novel treatment medicine is provided for the calcified aortic valve disease.

A biological nanoparticle for targeted therapeutic delivery is provided. The biological nanoparticle includes a plurality of extracellular vesicles. Characteristically, each extracellular vesicle contains a microRNA, which has been modified with ExoMotif sequences to enhance selective loading into the vesicles during their biogenesis. The microRNA is configured to inhibit plaque formation and vascular calcification. Additionally, a hydroxyapatite-binding peptide (HABP) is conjugated to the surface of each extracellular vesicle, facilitating targeted delivery to calcified tissues. This configuration enables the nanoparticle to specifically target areas of vascular calcification, where it can deliver the microRNA to modulate cellular behavior.

The invention discloses low-temperature injectable hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. Hydrogel is prepared from high-molecular-weight hyaluronic acid grafted with vitamin E and poly (N-isopropylacrylamide), the hydrogel is in an injectable liquid state at 4 DEG C, and a drug capsaicin (CPZ) wrapped by micelle is loaded and injected into the brain from the brain in a stereotactic mode. The pharmaceutical composition can temporarily maintain local low temperature of brain tissue and release capsaicin, reduce local metabolic activity of ischemic brain tissue, temporarily inhibit biogenesis, reduce NADH, FADH2 and other reducing equivalents and ADP accumulation, reduce the possibility of electron leakage in an electron transport chain, and improve the bioavailability of ischemic brain tissue. Vitamin E is added to reduce superoxide anions (O2 <.->) generated by reduction reaction of oxygen and leaked electrons in the reperfusion process, and vitamin E can promote ROS removal, synergistically alleviate oxidative stress and subsequent inflammation, and comprehensively improve cerebral ischemia reperfusion injury.

The application provides a fungal tangeretin derivative tangeretin A, and a structural formula of the compound is The tangeretin A provided by the application is a tangeretin derivative with a novel skeleton structure, is a tetraloop novel skeleton type, has a rare spiro skeleton, is a polymer formed by a quinone tangeretin and 2-hexene 1,3-diene-2,5-dimethyl furanone in terms of biogenesis, and can be applied to preparation of human pathogenic bacteria and an agricultural antibiotic precursor, and lays a material foundation for next-step biological medicine and pesticide research and application of an antibacterial component.

The invention discloses an engineered extracellular vesicle capable of efficiently delivering circular RNA (Ribonucleic Acid) based on an intracellular cyclization technology as well as a construction method and application of the engineered extracellular vesicle. A circRNA expression vector is designed on the basis of a tRNA shearing mediated cyclization mechanism, and the sequence of the circRNA expression vector is systematically optimized, so that the transcription and translation efficiency in mammalian cells is improved. Secondly, the EV membrane is subjected to engineering modification by displaying an RNA binding protein capable of recognizing and enriching circRNA. Thirdly, biogenesis and secretion of the EV are enhanced by co-expressing the key regulatory gene. By integrating these methods, a robust platform is established for high-yield encapsulation and functional delivery of circRNA, and has been verified by in vivo studies. In a word, the work provides an extensible and efficient engineering framework for circRNA treatment based on EV, and opens up a new way for RNA delivery.

Described are microfluidic high-throughput single cell transwell systems for culturing single paired cells for real time monitoring of exosomes biogenesis, transportation and internalization between paired single cells.

This invention discloses a cultivation experimental device suitable for studying the biogenesis mechanism of maize rRNA, belonging to the field of cultivation experimental technology for the biogenesis mechanism of maize rRNA. It includes an experimental platform with a refrigerator at its bottom. A connecting plate is located on one side of the refrigerator, and a cultivation experimental chamber is located on the side of the connecting plate away from the refrigerator. The cultivation experimental chamber has a cultivation tank inside, and a first rotating shaft is rotatably connected to one side of the cultivation tank. This invention, through a series of structural features, creates a dynamic cultivation environment by rotating the platform, precisely matching molecular events and significantly improving the scientific rigor of experimental data. Secondly, it employs a closed-loop precision liquid injection system, allowing for the addition of culture medium without removing the dish, thus improving operational efficiency and avoiding the impact of environmental fluctuations on cell state, ensuring experimental stability and consistency.

The present disclosure provides compositions and methods for increasing mitochondrial function. The present disclosure further provides nanomaterial structures comprising high atomic vacancy concentrations which are used to stimulate mitochondrial production. The resulting mitochondria may be used for mitochondrial transplantation to treat diseases or disorders associated with decreased mitochondrial function.