34 results about "Ischemic brain" patented technology

The invention belongs to the technical field of biological medicines, and particularly relates to application of a targeted liposome as an active component in preparation of a medicine for treating ischemic brain injury. The targeted lipidosome for treating cerebral arterial thrombosis is prepared by fusing the lipidosome and the platelet-neutrophil aggregate membrane, realizes efficient and specific targeting of an ischemic brain region, effectively reduces brain tissue infarction and damage of a new region, relieves brain inflammation through multi-channel regulation and control, and improves the cerebral arterial thrombosis treatment effect. The compound can be used as an active ingredient for preparing a medicine for treating ischemic brain injury. And after the target liposome is further coated with a rutin drug, the synergistic treatment effect is realized. The liposome medicine can be used as an active component and has a good application prospect in treatment of ischemic brain injury diseases.

The invention discloses application of (20S)-ginsenoside Rh1 in preparation of drugs for preventing and / or improving cerebral arterial thrombosis, and relates to the technical field of medical application of natural drugs. The invention verifies that (20S)-ginsenoside Rh1 up-regulates the expression of an anti-apoptosis gene by promoting STAT3 phosphorylation and translocation to a cell nucleus, so that the (20S)-ginsenoside Rh1 plays a role in protecting neuronal injury induced by sugar oxygen deprivation. In MCAO model mice, the (20S)-ginsenoside Rh1 obviously relieves the symptom of ischemic cerebral apoplexy and has positive influence on neurodegenerative events related to cerebral infarction, and the anti-apoptosis and neuroprotection effects are most prominent. Mechanism research shows that (20S)-ginsenoside Rh1 plays a therapeutic role in PC12 cell and MCAO model mice by activating a JAK2 / STAT3 signal channel.

The present invention discloses the use of cytisine-N-methylene-(5,7,4'-trihydroxy) isoflavone in the preparation of a drug for treating cerebral infarction. The invention relates to the fields of medicine, natural products, traditional Chinese medicine, and functional foods. The compound has both alkaloid and isoflavone structures, a novel structure, and good drugability. It also contains both flavonoid and alkaloid structural characteristics and is combined with any excipient or carrier acceptable in pharmaceutical, nutritional, and food science. The compound of the present invention can be used as an active ingredient in the treatment of cerebral infarction, inhibiting ischemic brain damage. It not only has a significant therapeutic effect on cerebral infarction, but also possesses multi-level, multi-target comprehensive pharmacological effects and targeted treatment of various pathological mechanisms.

Disclosed is a non-invasive system and related methods to deliver electrical current to ischemic brain tissue rapidly with minimal set up, to treat acute ischemic stroke via direct cytoprotection and by collateral blood flow enhancement promoting recanalization of occluded vessel. In one embodiment, the system includes a cap with plurality of electrodes which are operable in combination to deliver an electrical current when in contact onto skin of the head region of the subject. In one embodiment, the electrical current delivery is individualized to target each individual's ischemic tissue only. In one embodiment, the electrode positioning can be selected from pre-determined electrode montages according to the location of the ischemia and vessel occlusion site. In one embodiment, the system can be applied as a standalone treatment or adjunct to reperfusion therapies to salvage the at-risk ischemic tissue (aka penumbra) and improve patient outcomes.

The present application discloses a method, apparatus, device and medium for constructing a spatial knowledge graph database of stroke, relating to the technical field of big data analysis. The present application utilizes the Tongyi Qianwen 2.1 model to deeply mine and analyze publicly available papers, identify and label the spatial distribution of genes, physiological regions, and pathological regions related to ischemic stroke, and display them in the form of a spatial knowledge graph database of stroke. The present application utilizes the natural language processing ability of the Tongyi Qianwen 2.1 model to automatically extract genes and their spatial distribution information from the literature and constructs a dynamically updated knowledge graph, solving the problems of low data integration efficiency, insufficient annotation accuracy, and insufficient dynamic association in the study of stroke spatial heterogeneity in the prior art.

This invention relates to the fields of pharmaceuticals and nanomedicine, specifically disclosing the preparation of a dual-targeting liposome drug delivery system and its application in stroke. The dual-targeting liposome drug delivery system comprises neutrophils, artemisinin, edaravone, and liposomes. The liposomes co-load artemisinin and edaravone, and their surfaces are modified with sialic acid derivatives and phosphatidylserine to form liposome nanomedicines. The neutrophils act as carriers, transporting the drug across the brain border (BBB) ​​and targeting it to the ischemic brain region. This dual-targeting strategy achieves precise two-stage drug delivery to the ischemic brain region. This delivery system can inhibit NLRP3 inflammasome activation, reduce pyroptosis, and promote microglial polarization towards the M2 anti-inflammatory phenotype, thereby effectively treating cerebral ischemia-reperfusion injury or ischemic stroke.

The invention discloses low-temperature injectable hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. Hydrogel is prepared from high-molecular-weight hyaluronic acid grafted with vitamin E and poly (N-isopropylacrylamide), the hydrogel is in an injectable liquid state at 4 DEG C, and a drug capsaicin (CPZ) wrapped by micelle is loaded and injected into the brain from the brain in a stereotactic mode. The pharmaceutical composition can temporarily maintain local low temperature of brain tissue and release capsaicin, reduce local metabolic activity of ischemic brain tissue, temporarily inhibit biogenesis, reduce NADH, FADH2 and other reducing equivalents and ADP accumulation, reduce the possibility of electron leakage in an electron transport chain, and improve the bioavailability of ischemic brain tissue. Vitamin E is added to reduce superoxide anions (O2 <.->) generated by reduction reaction of oxygen and leaked electrons in the reperfusion process, and vitamin E can promote ROS removal, synergistically alleviate oxidative stress and subsequent inflammation, and comprehensively improve cerebral ischemia reperfusion injury.

The invention discloses a traditional Chinese medicine monomer nanoparticle oral delivery system based on intestinal liver circulation and a preparation method thereof, spherical nanoparticles (SA NPs) with the particle size of 194 + / -20 nm are formed through self-assembly of threat resin acid (SA), and the limitation of a traditional nano carrier is broken through: the oral bioavailability reaches 34.7% by utilizing an ASBT-mediated intestinal liver circulation mechanism; the gastric acid environment is tolerated (the pH is stable at 1.5), and after penetrating through the intestinal epithelium, the drug is targeted to ischemic brain tissues; the medicine has carrier-medicine dual functions, and the cerebral infarction volume is reduced by 34.1% when the medicine is singly used; after the neuroprotective peptide NA1 is loaded (the drug loading capacity is 5.8 wt%), the infarct volume is reduced by 68.7% through synergism. The system is suitable for pre-hospital first aid of cerebral apoplexy, and the freeze-dried powder can be stored at normal temperature for 12 months. The delivery system has a great application prospect in preparation of drugs for treating acute ischemic stroke needing rapid brain targeting.

This invention discloses a brain-targeting neutrophil membrane-encapsulated tanshinone IIa PLGA nanoparticle formulation, its preparation method, and its uses. The brain-targeting tanshinone IIa nanoparticle formulation, Neu-Tan-IIa@PLGA, is characterized by comprising a PLGA-loaded tanshinone IIa nanoparticle core (Tan-IIa@PLGA) and an outer neutrophil membrane coating. This invention effectively overcomes the problems of poor water solubility, low oral bioavailability, and difficulty in crossing the blood-brain barrier associated with tanshinone IIa. This invention provides a tanshinone IIa with strong targeting and high drug stability, enabling delivery to lesions in ischemic brain regions and improving the safety and efficacy of stroke treatment.

The present invention relates to a composition containing gintonin as an active ingredient for the prevention and treatment of ischemic cerebrovascular disorders, including cerebral infarction. The gintonin of the present invention can reduce the volume of cerebral infarction and increase survival rates after cerebral infarction, making it useful for the prevention and treatment of cerebral infarction. Furthermore, pretreatment of human brain microvascular endothelial cells (HBMECs) with gintonin has been shown to have neuroprotective and injury-preventing effects, while posttreatment with gintonin has been shown to have neuroprotective and therapeutic effects. Mechanism verification revealed that gintonin is effective in restoring reduced nitric oxide (NO) levels, which are known to be involved in the onset and pathophysiology of cerebral infarction. Therefore, the use of the present invention may improve the prevention and treatment of ischemic cerebrovascular disorders, including cerebral infarction, and reduce medical costs.

A method for treating brain injury in a subject comprises administering a therapeutically effective amount of a PKM2 (pyruvate kinase M2) protein variant. The protein variant is delivered systemically and can reduce tissue damage after ischemic brain tissue reperfusion. The methods specifically involve the use of G415R mutants of PKM2 protein variants. The treatment is suitable for brain injury caused by hemorrhagic stroke and ischemic stroke. The PKM2 protein variants are ideally administered intravenously within one hour of reperfusion to maximize their therapeutic efficacy.

The invention relates to systems and methods for predicting ischemic brain tissue fate from multi-phase CT-angiography. More specifically, systems and methods are provided that enable meaningful prediction of core, penumbra and perfusion from mCTA images using software that has been trained via machine learning to interpret mCTA images.

The invention relates to the technical field of active polypeptides, and discloses a polypeptide with ASIC1a selective inhibitory activity and application thereof. The amino acid sequence of the polypeptide is EDCIPKWKGCVNRHGDCCEGLECYVRR KSFEVCIPKTPKT, and the amino acid sequence of the polypeptide is shown in the specification. According to the polypeptide with ASIC1a selective inhibitory activity and the application thereof, the polypeptide can specifically inhibit channel current of ASIC1a and can effectively inhibit channel current of ASIC1b, the molecular weight is 4629.40 Da, and compared with a monoclonal antibody with large molecular weight, the polypeptide has good blood-brain barrier penetration rate, does not have remarkable cytotoxicity after being tested by experiments, and can be used for preparing a monoclonal antibody with ASIC1a selective inhibitory activity. The possibility of causing human immune response is low, potential unsafe factors are relatively few, and the compound can be used for preparing neuroprotective drugs for treating ischemic brain injury caused by cerebral arterial thrombosis.

The invention relates to a polypeptide of SEQ ID NO: 1 for use in the treatment of acute ischaemic stroke (AIS) or in the prevention of the complications of AIS in an adult mammalian subject. The polypeptide can be used in patients in whom AIS is followed by reperfusion of ischemic brain tissue, or in patients in whom AIS is not followed by reperfusion of ischemic brain tissue (patients with permanent cerebral ischemia).

The invention discloses a targeted delivery preparation as well as a preparation method and application thereof, and belongs to the technical field of biology and new medicine. The targeted delivery preparation comprises an agent A and an agent B in a ratio of (0.1-0.3) g: (2-5) mL; the agent A comprises nasal brain targeting peptide modified stem cell factor liposome freeze-dried powder and gel dry powder, the amino acid sequence of the nasal brain targeting peptide is as shown in SEQ ID NO.1, and the gel dry powder is formed by self-crosslinking mangiferin and rutin; and the agent B is a normal saline solution containing carboxymethyl chitosan. The targeted delivery preparation not only can meet long-time stable storage, but also can enhance the nasal-brain delivery efficiency of stem cell drugs. Besides, the targeted delivery preparation can be used independently or in combination with other treatment drugs, and is used for treating ischemic cerebral diseases, especially cerebral arterial thrombosis.

The invention belongs to the technical field of biological medicine, and particularly relates to application of phthalide isoquinoline alkaloid in corydalis amabilis to preparation of a medicine for resisting cerebral ischemia. According to the application disclosed by the invention, corydalis amabilis with definite anti-cerebral ischemia activity is taken as a research object, and the anti-cerebral ischemia activity target P21 protein activating enzyme 2 of the corydalis amabilis is obtained and identified from ischemic brain tissues of middle cerebral artery occlusion (MCAO) rats by using a molecular target'hooking 'technology based on thermal proteomics. Then, the inventor detects the binding force of a monomeric compound and a target protein in corydalis amabilis by using a surface plasmon resonance technology, and finally screens out a key substance phthalide isoquinoline alkaloid, which exerts the anti-cerebral ischemia activity, in corydalis amabilis.

The present application relates to the use of compound 1-[4-ethoxy-3-[5-(6,7-dihydro-1-methyl-7-oxy-3-n-propyl-1H-pyrazolo[4,3-d]pyrimidine)] benzenesulfonyl]-3,5-dimethylpiperazine, particularly in the preparation of drugs for the prevention and treatment of diseases caused by cerebral ischemia, particularly ischemic cerebral neuron damages and necrosis.

The invention belongs to the technical field of pharmacy, and particularly relates to application of 3-butyl-6-hydrazinoisobenzofuran-1-one in preparation of a medicine for treating cerebral arterial thrombosis. The structural formula of the 3-butyl-6-hydrazino isobenzofuran-1-ketone is as shown in the specification. The 3-butyl-6-hydrazino isobenzofuran-1-ketone prepared by the preparation method disclosed by the invention has excellent anti-platelet aggregation effect, neuroprotective activity and antithrombotic activity. Derivatives, isomers or optical isomers, racemate, pharmaceutical salts, hydrates, solvates and pharmaceutical carriers or excipients of the compound can be applied to preparation of drugs for preventing or treating cardiovascular and cerebrovascular diseases and complications thereof caused by thrombus. .

The present invention relates to a pharmaceutical composition for preventing or treating stroke, comprising a pressed extract, a solvent extract or a fraction of a solvent extract of Juglans mandshurica fruit. The Juglans mandshurica fruit exhibits a protective effect on brain tissue (brain cells) against ischemic brain tissue damage induced by middle cerebral artery occlusion and reduces infarction, and thus can be usefully used for a pharmaceutical composition or health functional food for preventing, ameliorating or treating stroke.

The invention discloses application of a reagent for detecting abundance of intestinal flora markers in preparation of a cerebral apoplexy in-vitro diagnosis product. The method comprises the following steps: performing 16S v4 region amplicon sequencing on bacterial components in faeces of healthy people and cerebral arterial thrombosis people to obtain the abundance of bacteria, finding out flora of 18 specific genus and species as microbial markers through screening operation, and constructing a cerebral arterial thrombosis risk prediction model. Verification queue data proves that the cerebral arterial thrombosis risk prediction model is high in prediction sensitivity and good in specificity, cerebral arterial thrombosis patients and healthy individuals can be effectively distinguished in multiple samples, and the accuracy is 93% or above, so that the flora can be used as a cerebral arterial thrombosis detection marker, and the cerebral arterial thrombosis risk prediction model can be used for detecting cerebral arterial thrombosis. A reagent for detecting the abundance of the intestinal flora marker can be used for preparing a cerebral arterial thrombosis in-vitro diagnosis kit, so that a foundation is laid for early diagnosis, prevention and treatment as well as research of cerebral arterial thrombosis.

The present application relates to a gene for regulating cholesterol metabolism and a micropeptide MP19 encoded thereby, and a use thereof, and also relates to a use of the micropeptide in the preparation of a drug or pharmaceutical composition for detecting, preventing, alleviating or treating diseases associated with abnormal cholesterol metabolism, said diseases comprising atherosclerosis, age-related macular degeneration, hyperlipidemia, non-alcoholic hepatitis, hepatocellular carcinoma, coronary heart disease, ischemic stroke, and obesity. By means of endogenous overexpression or exogenous synthesis of the micropeptide, diseases associated with abnormal cholesterol metabolism can be effectively prevented, alleviated, or treated.

This invention provides a drug delivery system and its application based on brain microvascular endothelial cells, comprising stimulated brain microvascular endothelial cells, wherein the stimulation is performed by culturing in a culture system containing supernatant of brain microvascular endothelial cells cultured under glucose and oxygen deprivation. This invention solves the problems of targeting and timeliness in treating blood-brain barrier damage after ischemic stroke under current technologies. It can regulate the BBB while supporting its function and structure, maintaining and repairing BBB function after reperfusion, thus improving the prognosis of stroke treatment. The cell preparations in this invention are widely available and easily obtained, and can rapidly repair the blood-brain barrier. Furthermore, the cell preparations in this invention can target ischemic brain sites and can be administered via simple intravenous injection, facilitating clinical operation.

The present invention belongs to the field of biomedicine technology, and in particular to the use of targeted liposomes as active ingredients in the preparation of medicines for treating ischemic brain injury. The targeted liposomes for treating ischemic stroke of the present invention are prepared by fusion of liposomes and platelet-neutrophil aggregate membranes, achieving efficient and specific targeting of ischemic brain areas, while effectively reducing brain tissue infarction and damage to new areas, and alleviating brain inflammation through multi-pathway regulation. It can be used as an active ingredient for the preparation of medicines for treating ischemic brain injury. When the targeted liposomes are further loaded with rutin drugs, a synergistic therapeutic effect is achieved. The liposome medicine of the present invention can be used as an active ingredient and has good application prospects in the treatment of ischemic brain injury diseases.

The invention relates to systems and methods for predicting ischemic brain tissue fate from multi-phase CT-angiography. More specifically, systems and methods are provided that enable meaningful prediction of core, penumbra and perfusion from mCTA images using software that has been trained via machine learning to interpret mCTA images.

The invention discloses a marker combination for predicting the mortality risk of a patient suffering from acute ischemic cerebral infarction after etiotyping and thrombus extraction and application of the marker combination, and relates to the technical field of medical treatment. The key points of the technical scheme are as follows: the application of the marker in pathogenesis typing of the acute ischemic cerebral infarction patient and / or prediction of the death risk of the acute ischemic cerebral infarction patient after thrombus extraction is provided; the marker is a protein combination ML15 which comprises AGER, TNFRSF13B, CRIM1, ESM1, LAMP3, PRELP, LY6D, IL17D, CNTNAP2, EPO, CLSTN2, NTF3, GAL, OMD and MATN2; the effects of realizing high-precision prediction of the death risk of the patient within one year after thrombectomy, providing a key window for early intervention and analyzing pathogenesis are achieved.

This invention relates to the application of compound ID-8 in the preparation of drugs for treating ischemic cerebrovascular diseases. This invention demonstrates the protective effect of ID-8 on ischemic brain. Research data shows that ID-8 can significantly reduce brain tissue damage and cell death caused by ischemic stroke, shrink infarct foci, and improve neurological function. Compound ID-8 can be used to prepare drugs for treating ischemic cerebrovascular diseases, can be combined with various excipients, can be combined with various existing drugs for treating ischemic cerebrovascular diseases, and is applicable to various dosage forms and routes of administration. This results in novel, safe, and effective drugs and treatments for ischemic cerebrovascular diseases that can reduce brain ischemia-related damage, and has significant clinical and social value.

The present invention relates to compounds of formula (I) and to the addition salts thereof with a pharmaceutically acceptable acid, for the treatment of non-traumatic acquired brain injury, wherein M represents NR 1 R 2 group, OR 1 group or SR 1 group, A represents NR 4 R 5 group, OR 10 group or a hydrogen atom, R 3 is (C1-C6)alkyl, (C1-C6)cycloalkyl, aryl, -CH2-aryl, CH2-(C1-C6)cycloalkyl or -CH2-heteroaryl. The present invention further relates to compounds of formula (I) wherein R 5 is (C1-C8)alkyl or (C1-C6)cycloalkyl, said alkyl and cycloalkyl being substituted by one, two or three -OCOR a groups.

The application relates to application of Danshensu borneol ester related substances in treatment of ischemic brain and cardiovascular diseases. In particular, the application relates to use of Danshensu borneol ester isomers and derivatives thereof in preparation of medicines for preventing and treating ischemic cerebrovascular diseases and cardiovascular diseases, and belongs to the technical field of medicines.

The invention provides a traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from the following raw material medicines in parts by weight: 10 parts of gastrodia elata, 10 parts of ligusticum wallichii, 15 parts of rhodiola rosea, 10 parts of radix cyathulae, 10 parts of radix puerariae, 10 parts of rhizoma anemones altaicae, 6 parts of fructus gardeniae praeparata and 15 parts of medicated leaven praeparata. The gastrodia elata in the formula has the effects of expanding brain and coronary artery, increasing cerebral blood flow and reducing cerebral vascular resistance; ligusticum wallichii, radix puerariae, rhodiola rosea and radix cyathulae have special effects of reducing platelet surface activity, resisting platelet aggregation, resisting coagulation and inhibiting thrombosis, and can improve cerebral blood circulation; the rhizoma anemones altaicae and fructus gardeniae praeparata can reduce the generation of inflammatory factors after cerebral infarction, relieve inflammatory response, delay the occurrence of brain cell apoptosis, promote the absorption of encephaledema and improve local blood circulation, thereby playing a role in protecting ischemic brain cells. All the medicines are combined to improve brain circulation, repair vascular intima, protect brain cells, recover brain blood supply and promote nerve cell function repair, so that the neurological impairment symptom of a patient is improved, and the life quality is improved.

The invention relates to the technical field of biological medicine, in particular to application of a pH / ROS dual-response lipid nano system in treatment of cerebral arterial thrombosis, the lipid nano system comprises a Prussian blue nano enzyme and a dynamic covalent liposome, and the dynamic covalent liposome is composed of omega-3 fatty acid modified lipid double tails; the lipid nano system penetrates through a blood brain barrier through passive diffusion and dynamic covalent bond mediated targeting effect under the conditions of an acidic microenvironment of an ischemic brain area and an active oxygen level rise, and is enriched at an ischemic focus, and the pH / ROS dual-response lipid nano system is applied to treatment of cerebral arterial thrombosis, and has a good application prospect in treatment of cerebral arterial thrombosis. The traditional Chinese medicine composition can significantly improve neurological function and pathological injury, reduce cerebral infarction area, protect neuron survival and promote functional recovery.