Use of polypeptides micro-molecule in preparing medicine for preventing and treating ischemic cerebrovascular disease

A cerebrovascular disease and small molecule technology is applied in the application field of polypeptide small molecules in the preparation of drugs for preventing and treating ischemic cerebrovascular disease, and can solve problems such as peptides that have not yet been seen

Active Publication Date: 2009-02-11
GUILIN EIGHT PLUS ONE PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, there has been no report on the use of this peptide in cerebrovascular diseases

Method used

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  • Use of polypeptides micro-molecule in preparing medicine for preventing and treating ischemic cerebrovascular disease
  • Use of polypeptides micro-molecule in preparing medicine for preventing and treating ischemic cerebrovascular disease
  • Use of polypeptides micro-molecule in preparing medicine for preventing and treating ischemic cerebrovascular disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment 1: The preparation method of polypeptide small molecule

[0012] 1. The amino acid sequence of the pentapeptide is shown in SEQ ID NO: 1: Met-Gln-Cys-Asn-Ser

[0013] 2. The amino acid sequence of the tripeptide is shown in SEQ ID NO: 2: Cys-Asn-Ser

[0014] The above pentapeptide and tripeptide molecules were synthesized by Shanghai Sangong Biotechnology Co., Ltd., with a purity of more than 95%.

Embodiment 2

[0015] Example 2: Animal experiment of small polypeptide molecules used in the treatment of ischemic cerebrovascular disease

[0016] The pharmacological experiment of making focal cerebral ischemia model by mouse suture method is a commonly used animal experiment to verify that drugs have the effect of preventing and treating ischemic cerebrovascular disease (Yin XM, Luo Y, Cao G, Bai L, Pei W, Kuharsky DK, Chen J.Bid-mediated mitochondrial pathway is critical to ischemic neuronalapoptosis and focal cerebral ischemia.J Biol Chem.2002 Nov1;277(44):42074-81.), so it was used to verify the effect of the polypeptide molecule on cerebral ischemia in experimental animals protective effect.

[0017] 1. Animals

[0018] C57 / 6BL mice, male, weighing 20-24 g, were reared in groups of 10 mice / cage, divided into 8 groups in total. Bought from the Shanghai Experimental Animal Center of the Chinese Academy of Sciences (animal certificate number: Zhongke Hudongguanzi No. 99-003). Raised ...

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Abstract

The invention relates to the technical field of biological medicines, which is an application of a small polypeptide molecule in preparing a drug for preventing ischemic cerebrovascular disease. The research of the invention finds out that polypeptide bioactive molecules and polypeptide spliceosomes extracted from the secretion of amoeba histolytica have the pharmacological action of preventing ischemic cerebrovascular disease. The pentapeptide and tripeptide are proved to be capable of remarkably reducing the brain damage to the mouse caused by ischemia-reperfusion and remarkably improving the neuro behavioral of the mouse after ischemia by the results of pharmacological experiment on mouse models with focal cerebral ischemia. Compared with the kindred protein drugs, the small polypeptide molecule has strong brain protection effects; in addition, molecular weight is small, thereby being easy to permeate blood-brain barrier and having remarkable advantage of brain permeability. Therefore, the small polypeptide molecule provided has good application prospect in preparing the drug for preventing the ischemic cerebrovascular disease.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of small polypeptide molecules in the preparation of drugs for the prevention and treatment of ischemic cerebrovascular diseases. Background technique [0002] Ischemic cerebrovascular disease (ischemic stroke) is one of the diseases with the highest morbidity, mortality and disability rate in the world, which greatly threatens the health and quality of life of human beings, especially the elderly, and has become a global Huge burden on society's health resources. Clinically, the mortality and disability rate of ischemic stroke has taken the first place among neurological diseases. At present, there are few effective drugs used in the treatment of ischemic cerebrovascular disease at home and abroad. Therefore, it is of great significance to carry out in-depth research on drug prevention and treatment of ischemic cerebrovascular disease. [0003] An anti-inf...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/08A61K38/06A61P9/10
Inventor 杨鹏远芮耀诚章越凡
Owner GUILIN EIGHT PLUS ONE PHARMA CO LTD
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