319 results about "Bioactive molecules" patented technology

The invention provides a stem cell exosome vesicle induced by traditional Chinese medicine functional components as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. According to the preparation method of the stem cell exosome vesicle, traditional Chinese medicine functional components serve as an exosome inducer, stem cells can be induced to proliferate and secrete exosome with the enhanced function, the yield of the exosome can be increased, bioactive molecules can be loaded, and the bioavailability of the exosome in diabetes treatment is remarkably improved. The stem cell exosome vesicle disclosed by the invention can be applied to treatment of diabetes and complications thereof, not only can improve functions of islet cells, but also can treat chronic inflammation, microangiopathy and other metabolic diseases related to diabetes, and has a wide clinical application prospect.

The invention discloses a preparation method of a pyrazolone amino-acid ester derivative. The pyrazolone amino-acid ester derivative is obtained through three-component cascade reaction by taking pyrazolone imine, a pentafluorophenylacetate derivative and alcohol as reactants and chiral isothiourea as a catalyst. According to the invention, challenges of competitive side reactions between a plurality of reaction substrates and intermediates and stereoselectivity control are overcome, the application range of the substrate combination of the existing system is broken through, and synthesis of a series of pyrazolone amino-acid ester derivatives is realized in a green and efficient manner. According to the method disclosed by the invention, substrate preassembly is not needed, transition metal catalysis is not needed, raw materials and the catalyst are cheap and easy to obtain, reaction conditions are mild, and the substrate application range is wide. In addition, the invention also provides a new way for introducing the medicine bioactive molecules into the synthesis of the pyrazolone amino-acid ester compound.

The invention belongs to the technical field of biology, and provides an electroporation buffer solution applied to exosome electroporation, the pH value is 6.8-9.8, the osmotic pressure is 80-320 mosm, water is used as a solvent, and the electroporation buffer solution comprises the following components: 15-25% w / v of iodixanol, 1.035-1.265 mM of KH2PO4, 22.5-27.5 mM of KCl, 0.18-0.22 mM of EDTA-2Na and 18-22 mM of HEPES. The electroporation buffer solution provided by the invention can improve the loading efficiency of the load and reduce damage to an exosome membrane structure and internal bioactive molecules in an electroporation process. The electroporation buffer solution can effectively reduce aggregation and fusion phenomena of exosomes after electroporation, and improves the stability and integrity of the exosomes. The electroporation buffer solution provided by the invention can improve the application degree of the exosome as a targeting drug delivery system.

Modified DDAH polypeptides and their uses thereof are provided. Exemplary embodiments provide DDAH polypeptides which include one or more amino acid substitutions, additions, or deletions with natural or non-naturally encoded amino acids, and / or linkage to other biologically active molecules including other DDAH polypeptides, as well as PKEM. Additionally, use of said DDAH polypeptides for treatment of disease, such as heart failure or renal disease, is also provided.

The invention discloses a multi-active targeting tumor nano-drug with a multimode imaging function and an immune activation effect and a preparation method thereof, and belongs to the technical field of nano-drugs. The preparation method comprises the following steps: coupling inorganic metal-based nano particles / nano drugs with a multimode imaging function or / and targeting active organic biomolecules with specific functions and specific therapeutic drugs through a silane cross-linking agent or a combination of the silane cross-linking agent and a carbodiimide condensing agent; the active targeting type organic-inorganic composite nano-drug aggregate with a multi-mode imaging function and an immune activation effect is prepared. Wherein the inorganic component is a metal-based heterostructure nanoparticle with a multimode imaging function, and the organic component is a bioactive molecule or / and a specific therapeutic drug. The obtained nano-drug aggregate is stable in system, uniform and controllable in size and good in biocompatibility, and has specific targeting, such as specific targeting liver cancer cells.

The invention relates to an antibody drug conjugate which is shown in a formula (I) and contains two or more bioactive molecules with different action mechanisms, a preparation method of the antibody drug conjugate, and application of the antibody drug conjugate in prevention and / or treatment of diseases related to abnormal cell activity, including but not limited to prevention and / or treatment of tumor diseases. # imgabs0 #

The invention relates to a small extracellular vesicle containing Klotho protein and / or mRNA as well as a preparation method and application of the small extracellular vesicle. The preparation method of the small extracellular vesicle comprises the following steps: S1, constructing a recombinant expression vector containing a Klotho protein coding gene; s2, introducing the recombinant expression vector into recipient cells to obtain a receptor cell strain with stable expression of the Klotho gene; and S3, culturing the receptor cell strain, collecting a culture supernatant, and extracting the small extracellular vesicles from the culture supernatant. The small extracellular vesicles containing the Klotho protein and / or mRNA can regulate and control the expression of endogenous Klotho, the expression profile of bioactive molecules in the small extracellular vesicles is also changed, the small extracellular vesicles are rich in various kidney protective proteins, miRNA and other bioactive molecules, and the treatment effect on AKI and AD is remarkably enhanced.

The invention provides a nucleic acid aptamer and a screening method thereof, the aptamer is of an oligonucleotide DNA structure, the nucleotide sequence of the nucleic acid aptamer is the nucleotide sequence of any DNA fragment as shown in SEQ ID NO: 1-9, and the nucleic acid aptamer can specifically target cardiac muscle cells (CMs). According to the invention, a new strategy can be provided for clinical treatment of cardiovascular diseases, and meanwhile, bioactive molecules such as microRNA (miRNA), small interfering RNA (small interfering RNA, siRNA), lipidosome, microspheres, microcapsules and the like can be carried to be used as an intracellular drug delivery tool.

The present disclosure relates to biologically active molecules comprising a single domain antibody (sdAb) that specifically binds to the extracellular domain of human IL10Ra, compositions comprising such antibodies, and methods of use thereof.

The invention relates to the technical field of food, medicine and health care products, and discloses a composition containing rosemary exosome vesicles and application of the composition in anti-inflammatory, the composition is formed by mixing plant cell-derived exosome vesicles and functional active ingredients, the composition comprises the following components in percentage by mass: 45-55% of rosemary exosome and 45-55% of turmeric exosome; the preparation method of the composition containing the rosemary exosome vesicles comprises the following steps: S1, taking a certain amount of fresh rosemary and turmeric plants, removing root hairs and impurities, washing the fresh plants with domestic drinking water, washing the fresh plants with purified water for three times, and finally washing the fresh plants with ultrapure water for three times for 3-5 minutes each time; the product can carry various bioactive molecules, transfer cell signals in a transboundary manner, participate in anti-inflammation, inhibit tumor cell proliferation and promote normal tissue repair, has an anti-inflammatory effect, inhibits release of inflammatory factors, improves the immune function of a body and adjusts the immune balance state, and can play a role in neovascularization diseases of the body.

This invention relates to modified T cells that inducibly express a bioactive molecule, such as IL-7, and constitutively expresses an antigen receptor, such as a T cell receptor or chimeric antigen receptor that binds to a tumour antigen. The modified T cells may comprise a nucleic acid construct that comprises a first nucleotide sequence encoding the bioactive molecule, a second nucleotide sequence encoding the antigen receptor; an inducible promoter operably linked to the first nucleotide sequence and a constitutive promoter operably linked to the second nucleotide. Nucleic acid constructs and vectors are provided, as well as T cells comprising such constructs and vectors and therapeutic methods and uses thereof.

The self-assembled nano material comprises a Janus-based nano tube, wherein the Janus-based nano tube comprises at least one compound represented by a formula I to a formula XII, or a pharmaceutically acceptable salt of the compound. Also described are compositions comprising the Janus-based nanotubes, comprising a biologically active molecule covalently or non-covalently attached to the self-assembled nanomaterial.

The invention discloses a synthesis method of a novel benzosuberone compound, and belongs to the field of organic synthesis. The preparation method comprises the following steps: uniformly stirring methyltriphenylphosphonium bromide in a solvent at room temperature, dropwise adding an n-hexane solution of n-butyllithium in an ice-water bath, stirring a reaction system at room temperature for 30-40 minutes after dropwise adding is completed, then adding an initial raw material phenanthrenequinone 1 in the ice-water bath, transferring the reaction system to 40 DEG C for reaction, and after the reaction is monitored to be complete by a TLC (Thin Layer Chromatography) dot plate, filtering, washing and drying to obtain the phenanthrenequinone 1. And pouring the reaction liquid into a silica gel chromatographic column, and simply filtering by using dichloromethane as an eluent to remove salt and phosphine oxide. And performing rotary evaporation to remove the organic solvent to obtain a crude product, and separating and purifying to obtain a target product 2. The method is short in reaction time and low in reaction cost, provides a practical method for obtaining the seven-membered rigid ring skeleton, and has potential value for synthesis of natural products and bioactive molecules.

The present disclosure relates to novel lipids for delivering one or more biologically active molecules to a subject. The present application also relates to a composition or a nanoparticle comprising said lipid, methods of preparing said lipid, use of said lipid and method of using said lipid.

The invention discloses a preparation method of stability-enhanced alginic acid-keratin-based double-coated nanoparticles. The preparation method comprises the following steps: carrying out controllable enzymolysis, enzyme deactivation, centrifugation and freeze-drying on keratin to obtain keratin nanoparticles formed by self-assembly; dispersing the keratin nanoparticles in an acidic buffer solution to carry out structure development, adding bioactive molecules, and adjusting the pH to be neutral to recombine and close the keratin nanoparticle structure to obtain a keratin nanoparticle solution loaded with the bioactive molecules; the preparation method comprises the following steps: taking keratin as a template, dispersing the keratin into a sodium alginate solution, dropwise adding into a calcium chloride solution, cross-linking to form a calcium alginate shell, dialyzing or centrifuging to remove free bioactive molecules, and freeze-drying to obtain the bioactive molecule loaded alginic acid-keratin-based double-coated nanoparticles. The invention also discloses double-coated nanoparticles and application thereof, through controllable enzymolysis and pH regulation and control, the self-assembly and bioactive molecule encapsulation of the keratin nanoparticles are improved, and the double-coated nanoparticles with regular shape, good dispersibility, good redissolvability and good environmental stability are formed.

The invention discloses a multifunctional cyclic peptide, and the structure of the multifunctional cyclic peptide is an end-to-end cyclization structure of tryptophan, leucine, lysine, arginine, tryptophan, leucine, arginine, lysine, leucine, tyrosine, lysine, phenylalanine, glycine and glycine. The multifunctional cyclic peptide has the advantages of the cyclic peptide, can be used as a novel bioactive molecule integrating antibacterial, anti-inflammatory and osteogenesis / tooth promoting functions, can meet various clinical requirements, and has a wide application prospect.

The invention provides a spiro butane indolone derivative as well as a preparation method and application thereof, and belongs to the technical field of organic chemical synthesis. The series cyclization coupling reaction of the N-iodo aryl dicyclobutyl amide compound and the vinyl pyridine compound is realized by utilizing a photocatalytic system, and the method is used for constructing the spiro butane indolone derivative containing the pyridine structure. The spirobutane indolone derivative synthesized by the invention has an inhibition effect on H1975 human lung adenocarcinoma cells; the preparation method provided by the invention is mild in condition and simple and convenient to operate, has economical steps, provides a convenient synthesis way for the complex bioactive molecules, and has a good application prospect.

A skin-permeable peptide, a derivative thereof, or a fragment thereof of the present disclosure effectively delivers skin bioactive molecules deep into the skin tissues through assembly or fusion methods, thereby providing outstanding skin permeability as well as excellent skin residual effect of bioactive materials to maximize bioactive efficacy. Accordingly, the skin-permeable peptide, derivative thereof, or fragment thereof may be widely used as an active ingredient of a pharmaceutical composition for external use and a functional cosmetic composition that target skin tissues including mucous membranes.

The invention provides a membrane-wrapped organelle-like system for directionally assembling membrane protein, which comprises a molecular framework and a closed lipid bilayer membrane wrapping the outer side of the molecular framework, the molecular framework is loaded with load molecules, the molecular framework directionally recombines the lipid bilayer membrane through a transmembrane region of the membrane protein, and the lipid bilayer membrane is loaded with the load molecules. The lipid bilayer membrane provides a barrier and protection for a molecular framework and loaded molecules, and the lipid bilayer membrane is provided with directional recognition molecules. According to the invention, a molecular framework wrapped by a lipid bilayer membrane is utilized, and a novel technology capable of simulating lipid vesicles of an organelle membrane framework is adopted, so that a brand-new directional delivery function on specific cells or specific organelles in the specific cells can be endowed. And oriented assembly of a membrane protein compound and effective loading of bioactive molecules are realized by using a process of chemically recombining the lipid bilayer membrane.

The present disclosure provides compositions and methods of recovering a policosanol or a sterol from a mixture comprising one or more fatty alcohols, aldehydes, fatty acids, and / or sterols. The mixture may be, for example, a lipid extract obtained from industrial fermentation processes (e.g., filter cake, distillate residue). The policosanol and / or sterol fraction recovered may be enriched in certain fatty alcohols (e.g., 1-octacosanol, 1-triacontanol, and / or 1-dotriacontanol), or enriched in certain sterols (e.g., ergosterol, campesterol, stigmasterol, and / or β-sitosterol). These bioactive molecules may be useful in the treatment or prevention of various metabolic conditions (e.g., obesity, dyslipidemia, cardiovascular conditions, and other chronic metabolic diseases).

The invention relates to the technical field of food gel, in particular to bamboo shoot insoluble dietary fiber, pea protein and alginate ternary composite hydrogel as well as a preparation method and application thereof. By adjusting the pH value of a reaction system (a pH driving method) and introducing the insoluble dietary fibers into the binary gel, the use of a chemical cross-linking agent is reduced, and the property of the hydrogel is improved. The finally obtained hydrogel has the following advantages: the structure becomes more compact and uniform, and the deformability is also reduced; the mechanical strength is good, and possibility is provided for encapsulation of bioactive molecules; higher thermal stability is shown; the crystallinity is higher, and the mechanical strength and the functional stability are improved; the water retention capability of a gel network in the structure can be enhanced, and the porous structure is beneficial to stabilizing water molecules.

We are disclosing derivatives of 5-hydroxylated polymethoxyflavones. The derivatives offer enhanced oral bioavailability and improved intestinal permeability. The derivatives of the invention release the biologically active 5-hydroxylated polymethoxyflavones that are activated by chemical or enzymatic processes in the GI Tract. The derivatives are constructed as bioreversible prodrugs containing the bioactive molecules covalently linked with lipids like fatty acids, glycerides or phospholipids. The derivatives are useful against inflammatory conditions and aging. Formulations including the derivatives are also disclosed.

The present invention relates to a fusion protein platform with improved half-life, which uses an IgG Fc region, an IgM region, a J chain, and / or a linker. The present invention also relates to a fusion protein produced through the platform, and in particular, to a fusion protein comprising an IgG Fc region, an IgM region, a J chain, a linker, and / or a biologically active molecule. The fusion protein platform according to the present invention can be used in combination with various biologically active molecules selected according to the purpose. In addition, the biologically active molecules included in the platform can have increased binding activity. Therefore, the fusion protein produced through the platform has excellent effect of recognizing and regulating the target, and there is little concern of unpredictable side effects due to non-specific binding. In addition, the half-life in the blood of the body is increased, so that the therapeutic effect to be achieved through the fusion protein can be maximized.

The invention belongs to the field of tendon tissue scaffold preparation, and provides a tendon tissue scaffold preparation method based on a decellularization technology. Although the existing decellularization technology can realize removal of cell components and retention of a matrix structure, contradiction still exists in the aspects of considering decellularization efficiency and matrix integrity. The method comprises three steps of freeze thawing treatment, trypsin digestion ultrasonic treatment and nuclease treatment, and perfect balance between decellularization efficiency and matrix retention is realized by optimizing freeze thawing parameters, regulating ultrasonic conditions and reasonably matching enzyme types and concentrations. According to the method, the cell structure can be accurately destroyed, the release of cell contents is promoted, and meanwhile, the extracellular matrix structure and bioactive molecules are protected. Experiments show that the tendon tissue scaffold prepared by the invention is thorough in cell removal, retains an extracellular matrix structure of natural tendons to the greatest extent, is closer to natural tissues in mechanical properties and biological functions, and provides a new thought for tendon repair research and application.

The present disclosure relates to biologically active molecules comprising a single domain antibody (sdAb) that specifically binds to the extracellular domain of human gp130, compositions comprising such antibodies, and methods of use thereof.

An injectable liquid containing bioactive molecules released from a powder comprising a neo-synthesized extracellular matrix for use in the treatment or prevention of cancer, in particular in the local treatment of solid tumors.

A pharmaceutical combination for subcutaneous administration and use thereof are provided. The pharmaceutical combination as described herein for subcutaneous administration includes a hyaluronidase and an antibody-drug conjugate. The antibody-drug conjugate includes a fragment of a bioactive molecule, and the bioactive molecule has moderate toxicity. The pharmaceutical combination can achieve a larger volume and a larger dose of administration, and the side reaction is not enhanced.

The invention discloses a fresh-keeping storage method of plum fruits, which is characterized in that a compact network structure of a shellac-pullulan-gellan gum basic composition effectively obstructs invasion of external microorganisms, and significantly slows down loss of water in the fruits, so that the plump appearance and hardness of the fruits are maintained, and the fruit quality is improved. The siRNA and the AVG wrapped by the lipidosome accurately interfere with the ripening and senescence process in the fruit from the molecular level, the siRNA specifically silences the gene expression of cell wall degradation key enzyme and delays the softening process of the fruit, and the AVG effectively blocks the biosynthesis pathway of ethylene and inhibits the start of an after-ripening signal, so that the ripening and senescence process of the fruit is delayed. The external formed physical film provides a stable microenvironment for internal bioactive molecules, protects the internal bioactive molecules from being degraded and prolongs the action time, and the internal molecular regulation delays the disintegration of cell structures, so that the external film can be attached more enduringly and exerts a barrier function, and the maintenance of the fruit quality is realized under a refrigeration condition.

The invention belongs to the technical field of biomedical materials, and discloses microgel assembly powder capable of rapidly stopping bleeding as well as a preparation method and application of the microgel assembly powder. The microgel assembly powder is composed of microgel powder and assembly agent powder, and the mass ratio of the microgel powder to the assembly agent powder is (1: 10)-(10: 1); the microgel powder comprises carboxymethylated and sodium trimetaphosphate cross-linked dual-modified polymer powder, and the assembling agent powder is polyphenol modified polymer powder. The microgel assembly powder can quickly absorb moisture in blood to concentrate the blood after being in contact with the blood, meanwhile, rich ortho-phenolic hydroxyl groups on the assembly agent can immediately form a hydrogen bond network among microgel, red blood cells and platelets, and the microgel assembly powder is assembled into hydrogel with excellent mechanical strength and adhesion performance. In addition, the powder can load bioactive molecule thrombin, activate multiple blood coagulation cascade reaction and promote formation of a fibrous protein network and blood clots, and through the synergistic effect of multiple hemostasis mechanisms, the powder shows rapid and effective hemostasis performance.

Phthalide lactones, as an important class of benzopentolactone skeleton, are widely distributed in natural products, medicinal plants and bioactive molecules, and have significant pharmacological activities and synthetic application potential. This kind of structure not only shows various biological activities such as antibacterial, anti-inflammatory, antitumor, antioxidant, but also is an indispensable core structural unit in many drug molecules and lead compounds. At the same time, cyano is a very valuable functional group in the field of organic synthesis and medicinal chemistry, with strong electron-withdrawing properties, strong structural stability and other advantages, which can effectively improve the electronic distribution, lipid solubility and drug performance of the molecule. In view of the important significance of phthalide lactones and cyano in organic synthesis and drug research and development, the present invention develops a synthetic method for efficiently constructing cyano-containing alkyl chain substituted phthalide lactone compounds. It has important value in medicinal chemistry and organic synthesis.