53 results about "Isotachis japonica" patented technology

Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from the bacterial microorganisms Shewanella japonica and Shewanella olleyana, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

The invention discloses a method for breeding high-yield and disease-resistant glutinous rice, and belongs to a crop breeding method. The method disclosed by the invention comprises the following steps: selecting disease-resistant and high-yield japonica rice as a female parent, selecting blast-resistant and high-yield round-grain glutinous rice as a male parent, and hybridizing the japonica rice with the glutinous rice to obtain a generation F1; carrying out manual screening, rice stripe disease identification and bacterial leaf blight artificial inoculation identification, and selfing in a generation F5; and further screening by identifying bacterial leaf blight, rice stripe disease and rice blast, and identifying quality and taste so as to obtain a target variety. By adopting the breeding method disclosed by the invention, the bred glutinous rice has the advantages of excellent quality, good taste and good commodification; the selected waxy material has the advantages of good plant type, good ripe phase, strong disease resistance, strong yielding ability, good yield stability, coordinated grain structure and strong auto-regulation ability, and ripe stage is combined with taste quality during yield identification; and thousand seed weight of the bred material is more than 26g after screening for years.

The invention discloses a novel indica-japonica hybrid gene introgression sheath blight resistance breeding method. The novel indica-japonica hybrid gene introgression sheath blight resistance breeding method includes the following steps that (1), an indica-japonica variety / system to be improved and a sheath blight resistance indica-japonica resistance source are selected; (2), the toxin tolerance concentration of the selected indica-japonica variety / system to be improved is roughly identified; (3), a three-way cross F1 hybrid is acquired through cross breeding; (4), row indica culture medium anther culture is conducted on the three-way cross F1 hybrid; (5), anther-cultured seedlings are planted in a large field, multiple indica-japonica anther culture families with the improved sheath blight resistance are acquired through single-seedling harvesting and planting, the different anther culture families are further sown in the large field, a parent of the indica-japonica variety / system to be improved serves as a contrast, and the new indica-japonica strain with the good agronomic character and the improved sheath blight resistance is screened out. By the adoption of the novel indica-japonica hybrid gene introgression sheath blight resistance breeding method, indica-japonica sheath blight resistance genes can specially and efficiently seep into indica-japonica, sheath blight resistance breeding can be finished about three years, a conventional indica-japonica hybrid sheath blight resistance breeding period is remarkably shortened, and the high breeding application value is achieved.

The invention relates to a three-step method of I-type sterility rice, wherein it comprises that: selecting plant, checking, and cultivating. The invention can improve seed purity, hold former characters and shorten cultivate time.

The invention relates to a molecular labeling method of a wide compatibility gene S5<n>, belonging to the field of molecular genetics. An InDel label S136 is designed according to a structural difference between the cloned wide compatibility gene S5<n> and a non-glutinous rice allelic gene Si and between the cloned wide compatibility gene S5<n> and a japonica rice allelic gene Sj and is separated from the wide compatibility gene S5<n> and the allelic genes thereof. The label can be utilized to accurately sieve and distinguish a material with the wide compatibility gene S5<n> from variety sources and can be used for the molecular labeling breeding of the wide compatibility gene S5<n> and the seed selection of a rice wide compatibility restorer.

The invention belongs to the technical field of biological engineering and discloses a method for improving rice potassium ion efflux antiporter. A function mutant strain of rice potassium ion efflux antiporter protein is obtained by a KEA1 gene for mutation control of rice potassium ion efflux and protein encoded by the gene; meanwhile, gamma rays are utilized to treat 9522 strain of japonica rice, and mutants of which the leaf veins are whitened are selected in F2 generation after the rice is planted so as to obtain the function mutant strain of the rice potassium ion efflux antiporter protein. By utilizing the characteristic that the protein participates in chloroplast potassium ion efflux antiporter and controls rice potassium ion transport by a transgenic technology, a new rice stress-resistant line is generated by inhibiting the expression of the protein, and has important application value in agricultural production.

The invention provides a rice blast resisting gene Pi-y43 (t) and SSR (simple sequence repeat) markers relative to the gene Pi-y43 (t), which belong to the field of biotechnology, and particularly relate to japonica rice cloud-led rice blast resisting gene Pi-y43 (t) and 5 pairs of SSR pleomorphism markers relative to the gene Pi-y43 (t). The gene is formed on rice blast broad spectrum resistance variety cloud-led secondary chromosome, and is obtained on the basis of fine positioning and map-based cloning of the japonica rice cloud-led paddy anti-rice blast gene Pi-y43 (t). Compared with that with a known resistance gene Pib, a strain containing the rice blast resisting gene Pi-y43 (t) is highly resistant to the cloud-led 'Sichuan-43' rice blast, and the Pib gene-containing single-gene identification system shows moderate resistance to the 'Sichuan-43'. The five pairs of SSR pleomorphism markers comprise OJL03, OJL08, FJK08, P4F05 and FJK42, and are tightly interlocked with a target gene, so that the selecting efficiency and the veracity of the auxiliary selective breeding of rice blast molecular markers can be preferably improved.

The invention belongs to the technical field of new plant varieties and discloses a screening and cultivation method for high-quality and high-efficiency single-season japonica rice in the middle andlower reaches of the Yangtze River. The method comprises: collecting japonica rice varieties, carrying out sowing on May 11th, transplanting rice seedlings on June 1st, carrying out high-yield cultivation unified management to obtain a growth period, plant height, highest seedling, effective ear, grain number per ear, 1000-grain weight, a seed setting rate, ear length and a yield index parameter,and measuring a polished rice rate, a whole polished rice rate, chalkiness, a chalky grain rate, amylose content, protein content and gel consistency through a GBT 17891-1999 method. The method utilizes a gray correlation analysis method and a weight correlation analysis method to comprehensively inspect the above indicators to evaluate the quality of japonica rice. Compared with the traditional screening method, the screening and cultivation method is scientific, comprehensively evaluates the quality of japonica rice, accurately provides the use variety for accurate production and services the production.

The invention relates to application of an OsPIL15 gene in regulating and controlling salt tolerance of rice. The method comprises the following steps: constructing an OsPIL15-OE overexpression vector and an OsPIL15-KO knockout vector, introducing a japonica rice variety Nipponbare by using an agrobacterium-mediated method, and screening to obtain an OsPIL15 overexpression transgenic line and a knockout mutant; experiments prove that the growth state of the rice mutant with the OsPIL15 gene knocked out is obviously better than that of wild Nipponbare and OsPIL15-OE overexpression plants after the rice mutant is subjected to stress treatment for 7 days by 200mM NaCl salt. According to the invention, gene editing of the OsPIL15 is carried out based on the CRISPR / Cas9 technology, and a simple and effective technical means is provided for rapid creation of a new salt-tolerant rice strain. The OsPIL15 gene has potential application value in improving the salt tolerance of the rice, can be utilized in production by utilizing a molecular improvement technology, and has important practical significance in high yield, stable yield and stress-resistant breeding of the rice.

The invention discloses a method for creating a red rice strain by targeting a Rc gene by using a gene edition technique. The method comprises the following steps: editing sequences of a rc gene frameshift mutation point by using a gene edition technique, screening and collecting an RC genotype with function recovery, and successfully improving rice colors of two materials, namely nonglutinous rice Gangyou 8518 and japonica rice Xiushui 134. By adopting the method, an efficient breeding mode is provided for creating red rice germplasm resources and culturing excellent red rice varieties.

The invention discloses a filtration assisting method of Indica and Japonica and a specific primer thereof. The filtration assisting method detects whether segments shown in (a) and (b) are deleted in a protein gene that is shown in sequence 4 of an encoding sequence table in a genome of rice to be detected, and if so, the rice to be detected is Indica candidate, otherwise the rice to be detected is Japonica candidate; wherein, (a) is a DNA segment of 717 to 855 nucleotide from 5'-end in the sequence 3 of the sequence table; and (b) is a DNA segment in a nucleotide sequence that has the homology degree of over 97 percent with sequences that are limited by (a). The filtration assisting method of Indica and Japonica subtypes has important guidance in filtering Indica and Japonica varieties or local varieties in an early development stage, acknowledging the genetic differentiation degree between Indica and Japonica, utilizing the advantages of hybrid varieties Indica and Japonica, and culturing high-yield and high-quality hybrid rice, and can consequently optimize hybrid combinations, quicken breeding speed and lower breeding cost, has the advantages of simple operation, low cost and short period, fits for popularization and application, and provides a fast selection method for the quality identification of rice subtypes Indica and Japonica.

The invention belongs to the technical field of rice genetic breeding, and discloses a method for rapidly and accurately breeding a semi-glutinous japonica rice restorer line. The method comprises the following steps that semi-glutinous conventional japonica rice Nanjing 9108 which contains a semi-glutinous gene wxmq but does not contain a male sterility fertility restorer gene Rfla is selected as a donor parent and a non-semi-glutinous japonica rice restorer line Ninghui 821 containing an Rfla gene is selected as a receptor parent, backcross and selfing are utilized, meanwhile, molecular markers are used for auxiliary selection, and semi-glutinous genes and male sterility fertility restorer genes are polymerized into the same variety to obtain the semi-glutinous japonica rice restorer line. According to the method, screening is conducted through a specific molecular marker rapid detection method, the breeding efficiency is improved through rapid, accurate and efficient transformation and molecular-assisted selection, effective polymerization of the semi-glutinous genes and restorer line genes is achieved in the japonica rice restorer line, and rapid application of semi-glutinous hybrid japonica rice in production is accelerated while a breeding process is accelerated.

The invention discloses a method for cultivating Oryza sativa L.ssp.indica and Oryza sativaL.ssp.japonica simultaneously by using Oryza rufipogon Griff.. The method comprises the steps of parent hybridization, embryo rescue, backcross, self-cross and identification of Oryza sativa L.ssp.indica and Oryza sativaL.ssp.japonica, wherein the parent hybridization is that Yuanjiang Oryza rufipogon Griff. is used as a male parent, Oryza sativa L.ssp.indica is used as the female parent, and a pollen mixture obtained by mixing anthers of dead Oryza sativa L.ssp.indica with pollens of Oryza rufipogon Griff. according to a mass ratio of 1: 1 is used for pollination; an embryo culture medium comprises modified MS, 8 g / L of agar powder and 10 g / L of sucrose and has the pH of 5.8-6.0. According to the invention, the method greatly improves the success rate of hybridization, rescues the immature embryos obtained through hybridization, and improves the success rate of distant hybridization of cultivated rice and Oryza rufipogon Griff. from 0-3% to 60%-70%; through the synergy of all steps, Oryza sativa L.ssp.indica and Oryza sativaL.ssp.japonica are obtained simultaneously; the method is simple and practicable; a new method and technical support are provided for the cultivation of endemic new strains of Oryza sativa L.ssp.indica and Oryza sativaL.ssp.japonica.

The invention belongs to the field of the plant biological technology and the gene engineering technology, and particularly relates to a gene for increasing the protein content of japonica rice and application, in other words, gene cloning and application of main effective QTL (qPC-10) controlling the protein content of endosperm of rice. The gene of rice qPC-10 has the DNA sequence shown as SEQ ID No.1, and the amino acid sequence of the coded protein of the gene of rice qPC-10 is shown as SEQ ID No.2. The QTLqPC-10 gene can control the protein content of rice and can be stably inherited. By using the gene in japonica rice, the protein content can be increased by 1%, and a novel rice variety with high nutrient value can be cultured.

The invention relates to the field of plant gene engineering, and in particular relates to a potassium-ammonium double-function transport molecule of rice and an application thereof. The gene order of the potassium-ammonium double-function transport molecule of rice is as shown in SEQ ID No.1. The invention firstly obtains an OsKAT3 gene by cloning in Nipponbare (Oryza sativa ssp.japonica), and verifies that the OsKAT3 gene of the rice has the function of high-efficiency K+ absorption; and furthermore, the gene also has the characteristic of high-efficiency NH4+ transport.

The invention discloses an ALS mutant type gene of japonica rice. The 406th nucleotide on an ALS gene sequence of the japonica rice is mutated into a nucleotide T from G. The invention further discloses an ALS mutant protein encoded by the ALS mutant type gene of the japonica rice and application of the ALS mutant protein. The mutant protein comes from a japonica rice mutant plant capable of resisting ALS inhibitor herbicides. Compared with a wild type ALS sequence, the protein sequence in the invention is mutated at the locus Gly136. Due to green plant expression, the protein sequence can resist (tolerate) the ALS inhibitor herbicides, particularly imidazolone herbicides.

The invention, which belongs to the technical field of plant genetic engineering, discloses a miR1866 gene for controlling rice seed germination, an overexpression vector and a preparation method thereof, a gRNA expression vector, and application thereof. According to the technical principle of CRISPR / Cas9 and high-expression miRNA, the expression of the miR1866 gene in rice is regulated and controlled. A small interference fragment of the miR1866 gene is designed, expression vectors of pH-Ubi-CAS9-miR1866 and pTCK303-miR1866 are constructed, a japonica rice variety Nipponbare is introduced byusing an agrobacterium-mediated method, screening is carried out by using hygromycin (Hyg) resistance marker to obtain a positive transgenic plant, and the mutation of the miR1866 and the expressionquantity of the miR1866 gene are identified respectively by sequencing and a qRT-PCR method. The result shows that a miR1866 mutant plant and a miR1866 high-expression plant are obtained respectively.Compared with the wild Nipponbare, the planted Nipponbare has the following beneficial effects: the seed germination rate of the miR1866 mutant plant is remarkably reduced and the seed germination rate of the miR1866 high-expression plant is remarkably increased.

The invention belongs to the technical field of molecular biology of genetic crops, and in particular relates to a molecular breeding method of a reproducible heterozygous female sterile restorer line and its application. In the present invention, the wide-compatibility gene S5n is used as a bridge to select and breed the japonica rice carrying S5i or the indica rice carrying S5j near-equal restorer lines; the temperature-sensitive sterile gene or the photosensitive sterility gene is introduced into the near-equal line of the S5i or S5j locus In the gene restorer line, a near-isogenic restorer line carrying S5i+ temperature-sensitive sterility gene or photo-reflex sterility gene, or S5j+ temperature-sensitive sterility gene or photo-reflex sterility gene is bred; through high temperature or short-day conditions, the seed production method , to realize the reproduction of heterozygous female sterile hybrid rice restorer lines. Since the restorer line selected by the present invention is female sterile but male fertile, it is not necessary to remove the male parent after pollination for seed production, realize mechanized harvesting for seed production, improve hybrid rice seed production efficiency, and provide technical support for hybrid rice mechanized seed production.

The invention provides a complete set of primer pairs for constructing a semi-glutinous japonica rice variety DNA (Deoxyribonucleic Acid) fingerprint library, which comprises 18 pairs of SNP (Single Nucleotide Polymorphism) four primer pairs and 36 pairs of InDel primer pairs; the 18 pairs of SNP four primer pairs respectively and specifically detect 18 rice SNP sites SNPjs0001 to SNPjs0018; and the 36 pairs of InDel primer pairs respectively and specifically detect 36 rice InDel sites InDeljs0001 to InDeljs0036. Related screening methods and application are also provided. The complete set of primer pairs for constructing the semi-glutinous japonica rice variety DNA fingerprint library disclosed by the invention can be used for constructing the semi-glutinous japonica rice variety DNA fingerprint library, can be used for identifying authenticity and purity of the semi-glutinous japonica rice varieties, and is ingenious in design, simple, convenient and rapid to use, low in cost, free of environmental influence and suitable for large-scale popularization and application.

The invention discloses a method for improving quality of erect panicle japonica rice and a molecular marker. The method comprises the following steps: identifying and screening low grain protein content QTL for rice germplasm resources to determine target rice variety resources; performing molecular marker-assisted selection on erect panicle japonica rice varieties to be improved according to thebreeding target, to obtain new japonica rice materials with excellent quality. The low grain protein content QTL adopts tightly linked molecular marker G1. The method can quickly and accurately obtain the new rice materials with excellent quality, has clear target and short breeding period, and is cost-saving, and the breeding process is more efficient.

The invention discloses application of rice RAG2 gene in improving quantitative character of rice. The rice allergen protein RAG2 is one type of soluble protein in rice seed storage protein; the applicant constructs the over-expression carrier of the gene and converts the japonica rice variety Zhonghua No.11 (ZH11) of rice, the seed of the obtained over-expression transgenic positive plant is increased, and the output is obviously higher than the output of the wild type; the contents of protein and fat in the rice are obviously increased, the content of starch is reduced, and the quality of the rice is improved. The gene has new application in culturing the good plant variety with high output and high quality, so as to provide new possibility for culturing the new crop variety with increased output and improved quality.

The invention discloses a breeding method using a tetraploid recombination inbred lines hybridized / doubled between rice indica japonica subspecies. The breeding method includes the steps that indica rice is hybridized with japonica rice; doubling is carried out to obtain a tetraploid; the tetraploid is inbred, and tetraploid with target traits is selected for recombinant inbred lines; and a diploid with target traits is obtained by means of chromosome set ploidy reduction, that is, a rice breeding material with the targeted traits. The sterility of indica japonica F1 hybrids is overcome, the high tolerance of the tetraploid to the instability of a chromosome set is used to take the tetraploid as a platform that can quickly induce a large number of genomic mutations, the tetraploid recombination inbred lines with chromosome set variation such as part of homologous chromosome fragment exchange in the chromosome set and diverse phenotypes are quickly obtained from inbred progeny, and a diploid restorer induced by the chromosome set ploidy reduction method can mostly inherit the phenotype and resistance characteristics of their parental tetraploid. The breeding method has important significance for rice breeding.

The invention relates to an efficient japonica hybrid rice selective-breeding method capable of reducing seed production cost. The method comprises the steps of irradiating a bone restorer line and amaintainer line of japonica hybrid rice with 350Gy of 60Co gamma-rays, carrying out parcel planting, carrying out genetic background selecting on a mutated line of a heading stage by using 48 SSR markers, constructing heading stage libraries of the bone restorer line and the maintainer line of the japonica hybrid rice with regard to pressurized screening of major breeding characters, and subjecting the maintainer line and a corresponding BT japonica rice sterile line to backcross transferring, so as to establish a bone sterile line library of the japonica hybrid rice. Through matched screeningof a test-cross sterile line / restorer line and a restorer line / sterile line close to a heading stage in the heading stage library, a high-quality high-yield disease-resistant japonica hybrid rice newcombination is selectively bred. According to the method, the efficiency of library establishing is high, the heading stage library of the bone restorer line can be established generally in 3 to 4 generations, the heading stage library of the bone sterile line can be established generally in 5 to 6 generations, and library establishing parents reserve major characteristics and excellent combiningability of original parents.

The invention relates to a method for breeding half-bending heavy-panicle large-grain type japonica rice. The method comprises implementation steps of (I) establishing the property standard of the half-bending heavy-panicle large-grain type japonica rice; (II) performing introduction, evaluation, screening and utilization of variety resources; (III) performing breeding of bending heavy-panicle type superior-stress-tolerance high-yield varieties and half-bending panicle type superior-stress-tolerance high-yield varieties; (IV) performing breeding of bending heavy-panicle large-grain type superior-stress-tolerance high-yield varieties; and (V) performing breeding of half-bending heavy-panicle large-grain type superior-stress-tolerance extra-high-yield varieties. Through the adoption of the method disclosed by the invention, the bred varieties concurrently have double advantages of erect-panicle type varieties and bending-panicle type varieties, comprehensive stress tolerance polymerization is realized, breeding objectives of being superior, tolerant to stress and extra-high in yield are achieved, the selection efficiency and the probability of directionally breeding objective traitsare greatly improved, and the application effects are quite significant.

The invention discloses the application of a male sterility gene OsDPW3 and a method for restoring fertility; using conventional genetic engineering methods or based on the CRISPR / Cas9 system, the OsDPW3 gene is knocked out, changed or inhibited, and the OsDPW3 is made in the wild-type rice background of japonica rice The level of gene expression is reduced, and then the male sterile line of rice is obtained. The invention also relates to a method for restoring rice fertility, which uses primers to amplify the OsDPW3 gene and transforms mutant plants by means of genetic transformation, which can restore the mutants to wild-type phenotypes. The rice male sterile line prepared by the invention has no abnormal phenotype in the rice vegetative growth period, but abnormalities appear in the anther development process in the reproductive growth period. If applied to hybrid breeding, the work of detasseling the female parent can be exempted, the production efficiency can be greatly improved, and the labor cost can be reduced. It has important applications in agricultural production.

The invention provides a method for cultivating "Northern Japonica to Southward" rice varieties, which includes the following steps: Ehd1 Design the CRISPR / Cas9 gene editing site in the starting region of the gene receiving domain and construct the gene editing vector to transform rice to achieve Ehd1 Site-directed mutagenesis of genes, obtained Ehd1 Gene frameshift mutation and in-frame deletion mutant strain in which 3 amino acids are deleted from the start region of the receiving domain. Experiments have shown that compared with the wild type, the growth period of the in-frame deletion mutant strain is moderately extended by about two weeks, and the growth period of the frameshift mutant is delayed by nearly one month. Through the method provided by the invention, japonica rice materials with different growth periods extended can be quickly obtained, and the ecological adaptability of existing excellent varieties can be expanded. It can provide an efficient gene knockout method and breeding method for the selection of japonica rice varieties that can adapt to different ecological conditions in low latitude areas.

The invention belongs to the field of crops, and particularly relates to an editing method and application of a japonica rice variety quality improvement breeding fragrance control and starch synthesis gene. The invention discloses a gene editing transformation vector, which takes pCambia3301 as a vector skeleton, and further comprises a ZmU6 promoter and a ZmUBI1 promoter, the vector also comprises a plurality of gRNA fragments designed for recognizable sites of different exon regions of Badh2 and Waxy genes; the gRNA fragments are located between the ZmU6 promoter and the ZmUBI1 promoter, and synchronous expression is performed through the ZmU6 promoter; and the gRNA fragments are interactively connected through a tRNA technology. According to the invention, a gene editing genetic transformation vector is constructed, an agrobacterium tumefaciens-mediated method is used for transforming a japonica rice variety, various editing of a nucleotide sequence of regenerated plant Badh2 and Waxy gene target regions are realized, and finally, a glutinous rice, non-transgenic and stably inherited homozygous edited rice strain with fragrance is obtained.

Provided is a pharmaceutical composition containing, as an active ingredient, a Sophora japonica L. extract, for the prevention and treatment of neurodegenerative disorders and improvement of cognitive functions. Particularly, it was confirmed that the Sophora japonica L. extract, according to the present invention, has a significant effect in an Alzheimer's Disease-causing mouse model, through a Y-maze test and a Morris water maze test, thereby confirming that the Sophora japonica L. extract could be useful as an active ingredient in the pharmaceutical composition for the prevention and treatment of neurodegenerative disorders and the improvement of cognitive functions.