Method for cultivating Oryza sativa L.ssp.indica and Oryza sativaL.ssp.japonica simultaneously by using Oryza rufipogon Griff.

A technology of wild rice and japonica rice, applied in botany equipment and methods, horticultural methods, applications, etc., can solve problems such as unsatisfactory breeding efficiency, no new varieties of japonica and indica rice, and no systematic cultivation technology of new rice strains. , to achieve the effect of convenient operation, simple method and improved survival rate

Inactive Publication Date: 2015-09-02
云南省农业科学院生物技术与种质资源研究所
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Problems solved by technology

However, this technology is still in the stage of partial gene application of wild rice, and no systematic new rice strain breeding technology has been formed, and the breedi...

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  • Method for cultivating Oryza sativa L.ssp.indica and Oryza sativaL.ssp.japonica simultaneously by using Oryza rufipogon Griff.
  • Method for cultivating Oryza sativa L.ssp.indica and Oryza sativaL.ssp.japonica simultaneously by using Oryza rufipogon Griff.
  • Method for cultivating Oryza sativa L.ssp.indica and Oryza sativaL.ssp.japonica simultaneously by using Oryza rufipogon Griff.

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[0017] The following examples are used to further illustrate the present invention, and each example is a conventional method without special instructions.

[0018] Test materials: Yuanjiang common wild rice (Oryza rufipogon Griff.) was directly sourced and originally sourced from Yunnan Province, China, and the original source time and direct source time were both in April 2005 (collection method). Yuanjiang common wild rice is considered by many experts to be one of the most primitive common wild rice types, and the differentiation of japonica and indica has begun. With many excellent genetic characteristics, it is an excellent species for improving cultivated rice. Japonica Hexi No. 35 (commercially available) is a high-yield, cold-resistant and blast-resistant variety bred by the Yunnan Academy of Agricultural Sciences. It has been widely used in Yunnan and surrounding suitable areas. Purchased by the company, Yunnan Datian Seed Industry Co., Ltd. Mailing address: Yungui ...

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Abstract

The invention discloses a method for cultivating Oryza sativa L.ssp.indica and Oryza sativaL.ssp.japonica simultaneously by using Oryza rufipogon Griff.. The method comprises the steps of parent hybridization, embryo rescue, backcross, self-cross and identification of Oryza sativa L.ssp.indica and Oryza sativaL.ssp.japonica, wherein the parent hybridization is that Yuanjiang Oryza rufipogon Griff. is used as a male parent, Oryza sativa L.ssp.indica is used as the female parent, and a pollen mixture obtained by mixing anthers of dead Oryza sativa L.ssp.indica with pollens of Oryza rufipogon Griff. according to a mass ratio of 1: 1 is used for pollination; an embryo culture medium comprises modified MS, 8 g/L of agar powder and 10 g/L of sucrose and has the pH of 5.8-6.0. According to the invention, the method greatly improves the success rate of hybridization, rescues the immature embryos obtained through hybridization, and improves the success rate of distant hybridization of cultivated rice and Oryza rufipogon Griff. from 0-3% to 60%-70%; through the synergy of all steps, Oryza sativa L.ssp.indica and Oryza sativaL.ssp.japonica are obtained simultaneously; the method is simple and practicable; a new method and technical support are provided for the cultivation of endemic new strains of Oryza sativa L.ssp.indica and Oryza sativaL.ssp.japonica.

Description

technical field [0001] The invention relates to a method for breeding japonica rice and indica rice, in particular to a method for simultaneously cultivating japonica rice and indica rice by using wild rice as a parent, and belongs to the technical field of crop breeding. Background technique [0002] Rice is the staple food crop of more than half of the world's population. The cultivated species are the Asian cultivated rice (Oryza sativa L.) which is widely cultivated in the world and the African cultivated rice (Oryza glaberrima steud.) which is limited to West Africa. In my country, the cultivated rice species are Rice cultivated in Asia is divided into two subspecies: indica rice (Oryza sativa L. ssp. indica) and japonica rice (Oryza sativa L. ssp. japonica). my country is a country that pays equal attention to japonica and indica in rice cultivation. As early as two thousand years ago, the existence of two subspecies of japonica and indica was recognized, and the layout...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H4/00
Inventor 程在全陈玲殷富有肖素勤张敦宇余腾琼李定琴钟巧芳柯学付坚陈越王玲仙蒋聪李娥贤将春苗罗红梅杨雅云曾民黄兴奇
Owner 云南省农业科学院生物技术与种质资源研究所
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