47 results about "Common wild rice" patented technology

The invention relates to the technical field of rapid propagation of plants, in particular to a rapid propagation method of Oryzarufipogon Griff by tissue culture. The method comprises the steps as follows: selecting and disinfecting rice seeds; inoculating the seeds into a culture medium containing seaweed extracts to obtain sterile test tube seedlings; inoculating the test tube seedlings into a medium containing aloe extracts to obtain cluster buds; inoculating the cluster buds into a medium containing wheat germ extracts to obtain robust plants; transplanting the robust plants into a medium containing chlorella extracts for culture to obtain complete plants with roots. Compared with a traditional method, the method has the advantages that by optimizing the medium at each stage of rapid propagation, the germination rate of the wild rice seeds is further increased, propagation and differentiation of the cluster buds are promoted, rooting capacity of new plants is enhanced, obtained new seedlings grow well and are robust, and the success rate of rapid propagation of tissue culture of wild rice is significantly increased.

The invention discloses a method for screening a Yuanjiang common wild rice introgression line material with high tissue culture ability. The method comprises the following steps of: 1) performing inducing culture on seeds of plant introgression lines to be identified to obtain calluses, and counting the inductivity; 2) sequentially subculturing the calluses obtained in step 1) twice to obtain first subcultured calluses and second subcultured calluses respectively, and counting the browning rate of the first subcultured calluses and the browning rate of the second subcultured calluses respectively; and 3) differentiating the calluses obtained in step 2), and counting the differentiation rate. The experiment proves that: Yuanjiang common wild rice and Teqing are utilized to construct an introgression line population, mature embryos of 80 introgression lines are cultured, and phenotype data of characters related to culture abilities of the introgression lines are investigated to screen the line with high culture ability by using the Teqing as control, so that a transgenic receptor with high culture ability can be provided for genetic improvement and functional genome research of rice.

The invention relates to a method for improving the culture efficiency of the anther of the filial generation of Oryza rufipogon, and belongs to the field of a plant tissue culture technology. The method for improving the culture efficiency of the anther of the filial generation of the Oryza rufipogon comprises the following steps: selecting an explant, sterilizing the explant, inducing an anther derived callus by use of fluid suspension, performing multiplication culture on the anther derived callus, performing differentiation culture on the anther derived callus, performing rooting culture on a differentiated seedling (anther cultured seedling), hardening the seedling and transplanting. According to the method, the anther is inoculated in a fluid nutrient medium to be subjected to suspension culture, the callus induction rate is improved, the callus is transferred onto a multiplying medium to be subjected to the multiplication culture, the quality of the callus is improved and the quantity of the callus is increased. The method has the advantages that the callus induction rate and the seedling survival rate in the anther culture of the filial generation of the Oryza rufipogon are improved greatly, the rice breeding process is accelerated, the breeding efficiency of the good rice variety is improved, and the method can provide a powerful technical support for acceleration of breeding of rice varieties by use of good genes of the Oryza rufipogon.

The invention discloses a molecular marker for a bacterial stripe resisting major gene BLS1 locus of rice and an application of the molecular marker. Specific steps for screening are as follows: (1) constructing a positioned segregation population, so as to obtain an approximate isogenic line F2 of the positioned segregation population; (2) extracting genomic DNA of rice leaves of each single strain of parents and a F2 population by adopting a CTAB method so as to carry out SSR molecular marker analysis; (3) preliminarily positioning a gene BLS1 in a region between RM19382 and RM510; (4) carrying out BLS1 close-linkage marking: carrying out detection and analysis after carrying out molecular marking by several kinds of marking primers, so as to position the BLS1 in a physical range, i.e., 21-kb between RM19400 and RM510. The molecular marker is applied to the selective breeding of bacterial stripe resisting rice varieties or the screening of resistant genetic resources. The molecular marker disclosed by the invention can be used for effectively detecting whether ordinary bacterial stripe resisting wild rice DP3 and derived varieties (lines) thereof contain the major gene locus or not, the efficiency of selection of bacterial stripe resisting rice is increased, and the bacterial stripe resisting rice varieties containing the gene BLS1 are obtained.

The invention discloses a common wild rice root specific promoter OrRSGp and application thereof. The invention provides a DNA molecule which is any one of NDA molecules of 1)-3) as follows: 1) a DNAmolecule of which an encoding zone is shown in sequence 1 in a sequence table; 2) a DNA molecule which is hybridized with a DNA sequence defined by 1) under a strict condition and has functions same as those of the DNA sequence; 3) a DNA molecule which has at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% of homology with the DNA sequence defined by 1) and has functions same as those ofthe DNA sequence. According to the common wild rice root specific promoter OrRSGp, one root specific promoter is obtained from a common wild rice genome through separation, and transgenosis GUS analysis shows that a segment of the promoter has a root-specific activity.