7 results about "Common wild rice" patented technology

The invention discloses a method for in-vitro preservation of common wild rice and Nwa wild rice, and belongs to the technical field of plant tissue culture. The method comprises the following steps: selecting a stem node with an axillary bud as an explant; soaking in 75% alcohol for 10-15 seconds, soaking in 10% 84 disinfectant for 15 minutes, performing vacuum treatment in 0.1% mercuric chloride solution under-0.1 Mpa for 5 minutes, and performing water bath at 40 DEG C for 20 minutes for combined sterilization; inserting the sterilized explant into a bud differentiation culture medium containing 2mg / L KT and 0.1 mg / L NAA, and culturing for 3-5 days under the conditions that the temperature is 23 DEG C and illumination is performed for 16 hours per day; cutting off buds growing to 3-5cm, and transferring the buds into a rooting culture medium; (5) carrying out test-tube plantlet propagation through stem node shearing or tillering cutting; the tissue culture seedlings with well developed root systems are hardened and then transplanted to a mixture of vermiculite and nutrient soil (v / v = 1 / 1). According to the method, the pollution rate is as low as 16.35%, the differentiation rate of the explant reaches 47.36%, the process is simple, convenient and efficient, the production period is short, the seed quality resources of common wild rice and Nilval wild rice can be effectively preserved, and the method has important significance on protection of precious wild rice resources.

The invention discloses a common wild rice OrLRR1 gene and application thereof in improving rice blast resistance of rice, and belongs to the technical field of genetic engineering and phytopathology. The nucleotide sequence of the oryza vulgaris OrLRR1 gene is as shown in SEQ ID NO: 1. It is found that the OrLRR1 gene plays an important role in rice blast resistance of rice. The deletion of the OrLRR1 gene reduces the resistance of the rice to the magnaporthe oryzae, and the over-expression of the OrLRR1 gene can improve the resistance of the rice to the magnaporthe oryzae. The invention provides an application of an OrLRR1 gene or an encoding protein thereof in improving the resistance of rice to rice blast, and a method for cultivating rice blast-resistant rice by regulating and controlling the expression of OrLRR1. The invention provides a new gene resource and a theoretical basis for cultivating a new rice variety with high rice blast resistance, and has important application value.

The present application relates to the field of plant bioengineering and plant improvement genetic engineering, and particularly relates to a miRNA capable of reducing the cadmium content of plants and application thereof. The miRNA is Dongxiang wild rice microRNA818a, the sequence of which is shown in SEQ ID NO. 1, and the precursor sequence is shown in SEQ ID NO. 2. The Dongxiang wild rice microRNA818a is derived from Oryza commom (O. Oryza rufipogon Griff.). The Dongxiang wild rice microRNA818a provided by the present application can significantly reduce the cadmium content in plants in a cadmium stress environment, provides key technical support and gene resources for molecular breeding of low-cadmium rice and other crops, and has important agricultural application value.

The invention relates to the field of plant bioengineering and plant improved genetic engineering, in particular to miRNA capable of reducing the cadmium content of plants and application of the miRNA. The miRNA is Dongxiang wild rice microRNA818a, the sequence of the miRNA is as shown in SEQ ID NO. 1, and the precursor sequence of the miRNA is as shown in SEQ ID NO. 2. The microRNA818a of the Dongxiang wild rice is derived from Oryza rufipogon Griff. The microRNA818a of the Dongxiang wild rice is derived from Oryza rufipogon Griff. The Dongxiang wild rice microRNA818a provided by the invention can significantly reduce the cadmium content in plants in a cadmium stress environment, provides key technical support and gene resources for molecular breeding of low-cadmium rice and other crops, and has important agricultural application value.

The application discloses a construction method of a common wild rice callus in-vitro regeneration system, and comprises the following steps: (1) obtaining an explant; (2) callus induction, wherein the induction culture medium is MS 4.74g / L+3% sucrose+0.3% plant gel+6-BA 0.5mg / L+2,4-D 2mg / L; (3) callus subculture; (4) induction of adventitious buds, wherein the differentiation culture medium is MS 4.74g / L+3% sucrose+0.3% plant gel+NAA 2mg / L+KT 1.5mg / L+6-BA 0.5mg / L; (5) strong seedling culture; (6) rooting culture; and (7) seedling transplanting. The application improves the induction germination rate and the proliferation coefficient. The test proves that the regenerated seedlings obtained by the application have the advantages of strong buds, strong main roots and high survival rate of seedling transplanting. The method for constructing the common wild rice regeneration system provided by the application provides strong technical support for the differentiation of common wild rice into seedlings through seed tissue culture and rapid propagation, and also provides an effective method for the protection and utilization of common wild rice.

The application provides a domestication gene THERMO TOLERANCE 5 (TT5) for regulating heat tolerance of plants and application thereof. In the application, a temperature-dependent Oryza minuta chromosome substitution line material for regulating rice quality and seed setting is identified, a QTL capable of polyfunctionally protecting yield and quality under high temperature is located and cloned by a method of map-based cloning, and the QTL is TT5. TT5 is a positive regulation transcription factor for heat tolerance, polyfunctionally regulates starch synthesis and storage protein accumulation, and endows high temperature tolerance. Cloning of TT5 has important significance for guiding de novo domestication of wild rice, and rapid domestication of wild rice can be realized by adding or erasing methylation of a genome.

This invention discloses the LOC_Os04g67314 gene of common wild rice and its application in improving rice resistance to rice blast, belonging to the field of genetic engineering technology. The nucleotide sequence of the common wild rice LOC_Os04g67314 gene is shown in SEQ ID NO: 1. Deletion of the common wild rice LOC_Os04g67314 gene reduces rice resistance to rice blast fungus, while overexpression of the common wild rice LOC_Os04g67314 gene can improve rice resistance to rice blast fungus. This invention provides the application of the common wild rice LOC_Os04g67314 gene in improving rice resistance to rice blast, and a method for cultivating rice blast-resistant rice by regulating the expression of common wild rice LOC_Os04g67314. This invention provides new gene resources and theoretical basis for cultivating new rice varieties with high resistance to rice blast, and has important application value.