Molecular seed breeding method by using single-fragment substitution line for pyramid-improving heat resistance during heading and flowering stage and filling stage of paddy rice

A technology for heading and flowering stage and molecular breeding, which is applied in the fields of plant genetic improvement, botanical equipment and methods, biochemical equipment and methods, etc., and can solve the problems of not easy stability, chain and burden of hybrid offspring, and restriction of breeding of heat-resistant varieties.

Inactive Publication Date: 2017-07-14
JIANGXI ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

Breeders have always wanted to introduce the heat-resistant genes in Yuanjiang wild rice into cultivated rice through interspecific hybridization

Method used

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  • Molecular seed breeding method by using single-fragment substitution line for pyramid-improving heat resistance during heading and flowering stage and filling stage of paddy rice
  • Molecular seed breeding method by using single-fragment substitution line for pyramid-improving heat resistance during heading and flowering stage and filling stage of paddy rice
  • Molecular seed breeding method by using single-fragment substitution line for pyramid-improving heat resistance during heading and flowering stage and filling stage of paddy rice

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Experimental program
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Embodiment 1

[0023] Embodiment 1 selects parents

[0024] The Super Rice Research and Development Center of the Rice National Engineering Laboratory (Nanchang) of the Jiangxi Academy of Agricultural Sciences used Shuhui 527 as the recurrent parent and Yuanjiang wild rice (Hehuatang No. 3) as the donor parent. SSR molecular marker-assisted selection covering the whole genome has bred a set of 112 single-segment replacement lines of Yuanjiang common wild rice with the background of Shuhui 527, and the genome coverage rate reached 73.5%. In 2010, on the first day after rice heading, plants with consistent growth were selected and put into pots and moved into artificial climate chambers. Three plants were planted in each pot, and three replicates were set up for each material, and the water and fertilizer were managed conventionally. Set the relative humidity to be 85%, the light to be 12h / d, and the constant temperature to be 38.0°C (day) / 28°C (night). Treat for 5 days. After treatment, mov...

Embodiment 2

[0027] (1) In the summer of 2011, a row of YJ07‐04‐06 and 9311 were planted at Nanchang Experimental Station of Jiangxi Academy of Agricultural Sciences, 9311 (CGMCC NO.12532) was the female parent, YJ07‐04‐06 (CGMCC NO.12534) and YJ01 -05-03 (CGMCCNO.12535) crossed F 1 Formulate hybrid combinations for male parents to produce F 1 , all mixed harvest after maturity. Planting 3 rows of F in Sanya, Hainan in the winter of 2011 1 , backcross to obtain BC 1 f 1 , all mixed harvest after maturity. Field management was carried out by conventional methods.

[0028] (2) Molecular marker-assisted selection: In the summer of 2012, 50 rows of BC were planted at the Nanchang Experimental Station of Jiangxi Academy of Agricultural Sciences 2 f 1 , 8 plants in each row, and the collected leaves of each plant were put into 2ml eppendorf tubes, filled with small steel balls, and then added 600 μl of 1.25% SDS extract solution after high-throughput tissue grinder high-speed vibration gr...

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Abstract

The invention belongs to molecular seed breeding and relates to a molecular seed breeding method for breeding a new paddy rice variety which has heat resistance during heading and flowering stage and filling stage. The method includes the steps of: 1) with a mid-indica two-line elite restorer line 9311, which is assigned the accession number of CGMCC No.12532, as a female parent, and an F1 generation, which is obtained by hybridizing Yuanjiang common wild rice single-fragment substitution lines YJ07-04-06 and YJ01-05-03 assigned the accession numbers of CGMCC No.12534 and CGMCC No.12535, as a male parent, performing intercross for 1 generation, performing backcross for 3 generations with a recurrent parent, and then performing selfing for 3 generations to obtain a stable homozygous plant strain BC3F4, wherein each generation is harvested in a single spike manner; and 2) identifying and selecting a new paddy rice variety by means of molecule marker assisted selection with combination of phytotron for simulating high temperature stress. By means of the method, a new paddy rice variety "Yuanye No.3" having heat resistance during the heading and flowering stage and filling stage has been cultured. Target characters are selected through molecule marker assistance, so that a restorer line having heat resistance during the heading and flowering stage and filling stage can be cultured within 3-4 years quickly and high-effectively.

Description

technical field [0001] The invention belongs to the field of molecular breeding, and relates to a molecular breeding method for improving rice heading and flowering stage and grain filling stage heat resistance by polymerization of single-segment substitution lines, in particular to a synchronous improvement of rice heading and flowering stage by polymerization of single-segment substitution lines of wild rice and molecular breeding methods for heat tolerance at the filling stage. Background technique [0002] High temperature heat damage is one of the main factors affecting the yield and quality of rice in the world, and it is also the main natural disaster of rice cultivation in China. It mainly occurs in the south of the Yangtze River Basin (Xiong Zhenmin, editor-in-chief Cai Hongfa. China Rice. Beijing: China Agricultural Science and Technology Press, 1992 ). High temperature disturbance in rice has the characteristics of sudden occurrence, large disaster area, and seve...

Claims

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Application Information

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IPC IPC(8): C12Q1/68A01H1/02A01H1/04
CPCC12Q1/6895A01H1/02A01H1/04C12Q2600/13C12Q2600/156
Inventor 曹志斌蔡耀辉聂元元毛凌华谢红卫李永辉徐斌
Owner JIANGXI ACAD OF AGRI SCI
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