32 results about "Single spike" patented technology

The invention relates to a device for producing a fluid containing a radioactive constituent, the device comprising: a shielded chamber (5) within which is located an isotope container (6) housing a radioactive isotope (7), the shielded chamber including first and second fluid connections (12,13) to opposing ends of the isotope container and a fluid conduit (14,15) extending from each of the first and second fluid connections to a fluid inlet (16) and a fluid outlet (17) respectively characterised in that the fluid inlet comprises a single spike (22) having a substantially circular cross-section, the spike being adapted to penetrate the rubber seal of a vial and the spike having two bores, the first bore extending from a first aperture adjacent the tip of the spike to a fluid connection with the fluid conduit and the second bore extending from a second, separate aperture in the spike to a filtering air inlet.

Apparatus and method are for extraction of follicular units. A dermal biopsy punch is utilized in combination with a suction device for extraction purposes. The use of suction to extract the follicular unit allows for distribution of the forces over a large area of the unit, helping to keep the unit intact. A perforating device, affixed to the punch, is utilized to weaken the structural integrity of the extraction area. The perforating device, having a single spike member which is inserted into the skin at a small, previously determined depth, in several locations, surrounding the follicular unit and reducing the holding tissue around the unit. The spike member of the perforating device is relatively thin and unlikely to damage the follicular unit. Because suction is utilized to extract the follicular unit, the distal end of the apparatus may have a guard which functions to prevent the extracted follicular units from being vacuumed further therein.

The invention particularly relates to a method for grade wheat scab disease, which comprises that follow steps: (A) acquiring an original image; B) carrying out grayscale processing, binarization processing, and opening and closing operation on that original image in turn, and obtaining a binary image; (c) combining that original image and the binary image; (D) Convertting to Lab color space, using IABC-K-PCNN method to process the a-channel gray scale image to obtain binary single-spike scab lesion pattern. (e) calculating a single ear area S1 and a lesion area S2, and then calculating a ratio R of that single ear area S1 and the lesion area S2; (F) according to the national standard, the ratio R, grading the disease of the single ear and outputting the disease grade; A grading device isalso disclosed. The disease grade grading method provided by the invention is tested by a large number of samples and is not destructive in sampling in field investigation of wheat scab, and has the advantages of reliable grading accuracy and high popularization value.

The invention discloses a fertilizing method for solving the problem of biennial bearing of paraphlonia. 1)after defoliation of paraphlonia in winter, branches of paraphlonia are retracted and trimmed to length of 10-30 cm, pit-expansion for fertilizing is carried out, root cutting treatment is carried out around the plants, annular trenches are dug, a fertilizer A and soil are uniformly mixed and applied in the trench; 2) after germinating of new tips in spring, when the sprout length of the paraphlonia is less than or equal to 5 cm, a fertilizer B is sprayed on the paraphlonia leaves, after the new shoot is grown to length of 15-25 cm, a fertilizer C is used for sprinkling the paraphlonia leaves, after the new shoot is grown to length of 25-40 cm, a fertilizer D is used for sprinkling the paraphlonia leaves. The paraphlonia is induced by the fertilizer B, the fertilizer C and the fertilizer D containing purslane herb, the nutrition-type meristematic tissue can be converted to inflorescence-type meristematic tissue, and floral induction is realized. rice hanging rate on single spike of paraphlonia can reach more than 95%, the problem of biennial bearing of paraphlonia can be effectively solved, output of paraphlonia is comprehensively increased, and the plantation benefit of paraphlonia is increased.

The invention provides a breeding method capable of resisting wheat yellow mosaic disease, and relates to the technical field of a crop breeding method. According to the breeding method, Ningmai 9 which is high in yellow mosaic disease resistance and high in excellent character combining ability is taken as a male parent, and Ningmai 8 or Yangmai variety which is high in yield and wide in adaptation and has the yellow mosaic disease is taken as a female parent, The method comprises the steps of carrying out hybridization on the male parent and the female parent to obtain F1; breeding the F1 to produce F2, F3 and F4, selecting excellent single spikes of the F2 and the F3, and carrying out blend threshing; selecting excellent single spikes of the F4, separating the spikes for threshing, and sowing head progeny rows F5 in a disease nursery; carrying out wheat yellow mosaic disease resistance identification on the F5, and selecting the head progeny rows which are high in resistance and good in agronomic trait; then, sowing the harvested head progeny rows in the disease nursery to form spike lines F6; repeatedly identifying the disease resistance of all the spike lines, and selecting the excellent spike lines; sowing the excellent spike lines of the F6 to form an evaluation nursery, and selecting the good lines which are disease-resistant, good in quality and high in yield. According to the breeding method, excellent genetic recombination and additive effects which are supplemented by disease resistance, agronomic trait, quality character and the like are used for directional selection of multiple generations, so that Ningmai 16 and a batch of budding new lines which are disease-resistant, good in quality and high in yield are cultured; the new lines are high in yellow mosaic disease resistance and has the advantages of having scab resistance and being high in yield potential, prominent in special quality, and the like, thus having production application values.

The embodiment of the invention adopts the following technical scheme, i.e. a novel summer corn SRP class group selfing line breeding method. The method is characterized in that F1 generation can be formed by hybridizing every two of a P class group corn, an Reid class group corn and an S class group corn; three or two F1 generations are hybridized to form an SRP class group; the SRP class group is subjected to back cross breeding or complex hybridization to breed a selfing line. The selfing line bred by the SRP class group breeding method has the characteristics of dense-resistance, disease resistance, lodging resistance and high combining ability; grains are hard grains or semi-hard grains; the self yield is that the grain weight of single spike is about 100g; the selfing line has the characteristics of compact plant type, dense planting resistance, high temperature resistance, rain waterlogging resistance, high adaptability to bad environment, and the like.

The invention discloses a multifunctional single-spike rice threshing machine. The multifunctional single-spike rice threshing machine comprises a rack. A threshing chamber internally provided with a threshing roller, a receiving chamber, an air flow type separation discharge barrel and a driving mechanism are arranged on the rack, wherein the receiving chamber is internally provided with a rotating twisting piece, and the driving mechanism is used for driving the threshing roller and the rotating twisting piece to rotate. A feed hopper is arranged on the top of the threshing chamber, the receiving chamber is communicated with the bottom of the threshing chamber, and the air flow type separation discharge barrel is provided with a top outlet in the top, a bottom outlet in the bottom and a side face inlet in the side face. The side face inlet is communicated with the receiving chamber, the top outlet is connected with an exhaust fan through a pipeline, an outlet of the exhaust fan is connected with an impurity discharge opening, and the bottom outlet is connected with a discharge port. The multifunctional single-spike rice threshing machine has the advantages of being simple in structure, convenient to operate, small in size, low in weight, good in threshing quality, high in efficiency and the like.

The invention relates to a spike-arranging seedling-breeding method of the rice three garden basic seed production. The present invention has the application steps that: (1) the selection of a fine single spike: strong and high anti-adversity single spikes 300-500 plants with the typical characters of the variety are selected as the next year ear-to-row garden material; (2) ear-to-row garden: after sterilizing the seed of the single spike selected in the last year, the branches are unfolded and the spikes are arranged and the breeding seedlings are placed; each spike seedling field occupies 30*20cm area, the selected spike rows, discarded rows, and fine variable spike rows are marked; the selected spike rows are numbered, and the harvest is performed by a single row, the threshing is performed separately, the seed is examined strictly as the spike series garden material; (3) spike series garden: the seedling of the selected spike rows last year is bred separately in every single spike row, and the seedlings into a sub-field which is called a spike series is transplanted; the spike series which cannot typically grow are discarded, the harvested selected fine spike series are mixed and threshed, and then the basic seed is obtained; (4) basic seed field: the seeds of the spike series harvested last year are seeded and mixed in the basic seed field, an isolation belt is arranged between varieties, to realize one variety one garden. The method is simple with obvious effect.

The invention provides a rice breeding method. Synchronous induced identification of rice blast and rice black-streaked dwarf virus is realized. The rice breeding method comprises the following steps: inducing the rice blast by using MingShui scented rice; attracting laodelphax striatellus to suck and propagate the rice black-streaked dwarf virus by using fragrant blood glutinous rice 2315; selecting and breeding group seeds and performing mixed-breeding and mixed-planting with the MingShui scented rice; after bacteria inoculation and virus inoculation are induced, performing field selection and indoor identification, and finally, selecting a single spike which meets a breeding target and performing generation-adding to form a high-quality high-yield rice blast-resistant and rice black-streaked dwarf virus-resistant new variety. According to the method, the breeding of the high-quality high-yield rice blast-resistant and rice black-streaked dwarf virus-resistant new variety can be accelerated, the disadvantages that the rice appearance quality of a variety which is popularized in current production is relatively poor, and the resistance to the rice blast and the resistance to the rice black-streaked dwarf virus are weak are overcome, so that the quality and the commodity characteristic of rice are improved, the using amount of a pesticide can also be reduced, environment pollution is reduced, the economic benefit is improved, and meanwhile, the requirements on green sustainable development industries which are espoused at present are met.

The invention aims at providing a marker for auxiliary detection of wheat high SNP (Single Nucleotide Polymorphism) and application thereof. The SNP marker is positioned on 553937825-th site nucleotide on a 3D chromosome of a hexaploidy ordinary wheat gene; the basic group of the SNP marker in a high-single-spike spikelet number wheat material is G; the basic group of the SNP marker in a low-single-spike spikelet number wheat material is A. The marker has the beneficial effects that the marker is a colinear marker; through digestion belt type, the typing can be accurately performed; through the typing detection on 164 individuals, the accurate rate reaches 85 percent.

The invention discloses a high-throughput heatproof rice screening method. The method comprises the following steps: (1) preparing F2 generation, DH line, BC1F2 or RIL seeds by using heat-sensitive rice in the flowering stage as a receptor and heatproof rice as a donor; (2) processing the seeds, carrying out tray close planting, cultivating seedlings to obtain a genetic segregation population, andplanting parents and F1 generation by using same processes; (3) reserving one effective tiller on every plant in the jointing and booting stage, carrying out heatproof screening treatment in an artificial climate room if the heading of the population is similar, and performing filing and fruit-setting in a normal environment when the heading and flowering of all the tillers of the populations arefinished; (4) performing pocket cutting and pile reserving after all plants are headed if the heading period is different significantly, and carrying out heading and heatproof screening; and (5) counting the heatproof fruit-setting rate after the dough stage of single spikes in order to complete the heatproof phenotype screening of the individual plants of the population. The method allows high-throughput heatproof screening to be quickly and reliably carried out by using the close planting control technology and pocket cutting.

The invention belongs to molecular seed breeding and relates to a molecular seed breeding method for breeding a new paddy rice variety which has heat resistance during heading and flowering stage and filling stage. The method includes the steps of: 1) with a mid-indica two-line elite restorer line 9311, which is assigned the accession number of CGMCC No.12532, as a female parent, and an F1 generation, which is obtained by hybridizing Yuanjiang common wild rice single-fragment substitution lines YJ07-04-06 and YJ01-05-03 assigned the accession numbers of CGMCC No.12534 and CGMCC No.12535, as a male parent, performing intercross for 1 generation, performing backcross for 3 generations with a recurrent parent, and then performing selfing for 3 generations to obtain a stable homozygous plant strain BC3F4, wherein each generation is harvested in a single spike manner; and 2) identifying and selecting a new paddy rice variety by means of molecule marker assisted selection with combination of phytotron for simulating high temperature stress. By means of the method, a new paddy rice variety "Yuanye No.3" having heat resistance during the heading and flowering stage and filling stage has been cultured. Target characters are selected through molecule marker assistance, so that a restorer line having heat resistance during the heading and flowering stage and filling stage can be cultured within 3-4 years quickly and high-effectively.

The invention relates to a method of purification rejuvenation and screening variation of wheat varieties. The method comprises screening year by year, wherein in first-year screening, a single spike with imperfect or diseased grains is eliminated from the original population of a wheat variety to plant a head progeny row nursery; in the head progeny row nursery, excellent head progeny rows which are remarkably different from main characteristics of improved varieties, have strong disease resistance and good comprehensive property are kept; after excellent head progeny rows are ripe, the whole row of single spikes of the excellent head progeny rows are harvested, diseased or imperfect single grains are eliminated, and the screened excellent head progeny rows are taken as a corresponding primary spike line of the head progeny rows; the eliminated seeds in the head progeny row nursery are mixed and collected, and are threshed to serve as original seeds for original seed production in the next year; and the screening idea of the first year can be repeated in the second, third and later years for constant screening.

The invention belongs to the field of crop molecular breeding, particularly relates to a molecular marker for regulating corn single spike weight main effect QTL, and also relates to a method for assisting the selection of corn single spike weight. The molecular marker for regulating the corn single spike weight main effect QTL is composed of two pairs of SSR markers of umc1120 and umc2134. The method for assisting the selection of the corn single spike weight comprises the following steps that the genome DNA of corns to be tested is extracted and PCR amplification is performed by using the umc1120 and umc2134 primers, when amplified products with the length of 338bp and 234bp are obtained, the corns to be tested are candidate large-spike-type corns. When the method is used to assist the selection of large-spike-type corn materials, only does the characteristic strip of the molecular marker need to be detected to predict the single spike weight size. The method is simple and feasible, and the selection efficiency is high. A large-spike-type corn individual plant can be identified during the corn growth early period, and the selection object is clear and not affected by the environment. A high-yield corn hybrid combination is designed accordingly.

A spike for dispensing fluids from a flexible bag, wherein the spike includes multiple fluid channels which transfer liquids from the bag to an enclosed dispensing chamber and air from the enclosed chamber to the bag to permit and control fluid flow within the system.

The invention discloses a breeding method of a fruit peel anthocyanin synthesized fresh corn selfing line. The method comprises the steps of carrying out hybridization line selection on a corn selfing line with peel rich in anthocyanin and a corn selfing line with colorless peel, carrying out selfing seed stock on a generation F1, ignoring the seed grain color character of a generation F2, carrying out selfing on single plants with relatively good phenotypic characters such as plant type, leaf type and disease resistance, preserving single spikes, concerning the seed grain color character from a generation F3, planting according to a head progeny row, simultaneously considering phenotypic characters such as the plant type, the leaf type and the disease resistance, selectively preserving single spikes with purple or black full-spike peel, planting according to the single spikes, continuing selfing for four generations, and preserving plant row with consistent characters and purple or black full-spike peel, so as to obtain the stably inherited fruit peel anthocyanin synthesized fresh corn selfing line. The breeding method can be used for assisting cultivation and authentication of the corn selfing line rich in anthocyanin and the breeding of new types of fresh corns rich in anthocyanin and has very good application prospects.

The method comprises: a) the emission of an ultrasound burst repeated at a predetermined recurrence frequency; and b) after each emission and for the duration of a time frame (n-1, n, n+1,...) separating two consecutive emissions, the reception of a plurality of successive signal spikes appearing in the course of the same frame. These spikes include spurious spikes (E'n-1, E'n, En+1,... ) originating from the emitter of another drone, and a useful spike (E'n-1, E'n, En+1,... ) corresponding to the distance to be estimated. To discriminate these spikes, the following steps are executed: c) for two consecutive frames, comparison of the instants of arrival of the p spikes of the current frame with the instants of arrival of the q spikes of the previous frame and determination, for each of the p.q pairs of spikes, of a corresponding relative time gap; d) application to the p.q gaps determined in step c) of a selection criterion making it possible to retain just a single spike of the current frame; and e) estimation of the distance as a function of the instant of arrival of the spike thus retained.

The invention discloses a paddy rice early flowering method, and belongs to the paddy rice breeding technical field; the method comprises the following steps: a, cutting 2-3 bright yellow single spikes with plump anthers at about 10:50 am; b, placing spike heads into clean water for immersion, and wetting the rice spike particles; c, knocking wet rice spikes on the palm so as to remove extra water, and shearing the rice spikes; d, gathering the sheared rice spikes and to-be pollinated hybridized female parents for bagging, closing the bag opening, and settling for 8-10 minutes; e, opening the bag and removing flowering male parent single spikes, and shaking the male parent single spikes so as to pollinate the female parents. The novel method allows the male parent paddy rice to flower in advance under low temperature, thus reducing work difficulty and intensity, and timely finishing paddy rice sexual hybridization work in the same day.

The invention relates to the technical field of rice breeding, in particular to a method for increasing the total grain number of a single spike of two-line hybrid early indica rice. An existing dual-purpose sterile line carrying photo-thermo sensitive genic male sterile genes serves as a female parent, apical spikelet 2-4-grain clustered early indica rice with a growth period not 2 days longer than that of the female parent serves as a male parent for hybridization, carrying of the photo-thermo sensitive genic male sterile genes and apical spikelet 2-4-grain clustering characters serve as breeding goals, clustered dual-purpose sterile line breeding is successively performed, and then conventional early indica rice in normal single-grain insertion is selected as a male parent to prepare atwo-line hybrid early indica rice combination. According to the two-line hybrid early indica rice combination obtained by the technology, the planted rice has the advantages that parts of apical spikelet 2-grains are clustered, the total grain number of the single spike is obviously increased, thousand seed weight and maturing rate are kept at normal level, high yield is achieved and the like, andper unit yield can be effectively improved.

The small miscellaneous grain thresher disclosed by the present invention can complete the threshing of small miscellaneous grain crops; Selection plate, fan-shaped collection cover, speed-regulating motor, frame, ground wheel, single-phase motor, centrifugal fan, collection port, and control handle for closing the concave plate sieve. Complete separation, and allow the grains to fall into the clearing part smoothly; use the difference in the mixed aerodynamic characteristics of rice grains and impurities to separate the grains from the impurities, blow the impurities out of the machine, and let the grains fall into the collection device to complete the entire threshing and collection operation; The rice single ear thresher has high working efficiency, convenient operation and simple structure, saves more labor resources, increases farmers' income, and reduces the labor intensity of workers and the probability of workers being injured.

The invention belongs to the field of molecule breeding and relates to a molecule breeding method for cultivating rice with heat resistance which is increased heading and flowering stage and grain filling stage. The method includes the following steps: a middle indica rice two-line elite restorer lines restorer 9311 with the accession number CGMCC No.12532 is served as female parent, single-segment substitution lines, YJ-07-04-06 and YJ01-05-03, of Yuanjiang common wild rice are hybridized to obtain a F1 as parent, the female parent and the parent are intercrossed for one generation, backcrossed is performed for three generations with the recurrent parent, and then self-cross is performed for three generations to obtain the stable homozygous plant line BC3F4, and each generation is harvested with single spike; and the new rice line is identified and obtained by the molecule mark auxiliary selection combined with the phytotron simulation high temperature stress. Yuan ye no.3 with substantial heat resistance of the rice heading flowering stage and grain filling stage is cultivated by utilizing the method. The character of the target is selected through the molecule mark auxiliary, and the heat resistance restorer of the heading and flowering stage and grain filling stage can be rapidly and efficiently cultivated in 3-4 years.

The invention discloses an identification method for rice grain sterility caused by high temperatures. The method comprises steps as follows: the humidity of a manual climate box is 70%-75%, and the mean daily temperature of high-temperature treatment is 33.0 DEG C; the mean daily temperature of contrast treatment is 27.0 DEG C; potting planting is adopted for rice; plants which ear and bloom in the same period are selected; high-temperature relative fructification is used as an identification index of high-temperature varieties; the mean fructification percentage of spikelet blooming on marked spikes during high-temperature treatment of the manual climate box is the high-temperature fructification percentage, and the mean fructification percentage of spikelet blooming on single spikes under the contrast temperature of the manual climate box is the contrast fructification percentage. The manual climate box is adopted, the reasonable temperature identification index is established, different high-temperature resistant rice varieties are screened out with certain cultivation management measures, a variety identification and heat damage evaluation method is provided for coping with rice high-temperature heat damage caused by climate warming, and the technical support is provided for reasonable layout of rice varieties and high-temperature heat damage control.

The invention relates to a traditional Chinese medicine preparation, and especially relates to a traditional Chinese medicine decoction used for treating acute bronchitis caused by wind heat affecting lung. The decoction is prepared from the raw materials of: 13-15g of kadsura pepper twig and leaf, 13-15g of dahurian azales leaf, 20-25g of single spike kyllinga herb, 4-6g of black cicada slough, and 4-6g of mix-fried licorice root. The decoction provided by the invention is characterized by low cost, easy preparation, low toxic and side effect, and relatively good treatment effect.