Molecular marker for regulating corn single spike weight main effect QTL and application method thereof

A molecular marker and corn technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problem of inconsistent results of QTL detection of single ear weight in corn, and achieve a simple and feasible identification method, Select the effect of high efficiency and clear selection goals

Active Publication Date: 2017-08-18
安徽英诺管理咨询有限公司
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Problems solved by technology

At present, more than 1,000 QTLs for yield-related traits have been included in the Maize Genome Database (MaizeGDB), and they are unevenly distributed in 10 linkage groups of maize. 2:3 , BC, RIL), the accuracy of the genetic linkage map used (generally >200 markers), and the detection environment of the positioning population, etc., there is no consistent result for the detection of the QTL of corn ear weight

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  • Molecular marker for regulating corn single spike weight main effect QTL and application method thereof
  • Molecular marker for regulating corn single spike weight main effect QTL and application method thereof
  • Molecular marker for regulating corn single spike weight main effect QTL and application method thereof

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Embodiment Construction

[0022] The present invention will be further described below in conjunction with specific examples. The test methods in the following examples are conventional test methods unless otherwise specified. The experimental reagents and consumables described in the following examples are all from conventional biochemical methods unless otherwise specified. Reagent company.

[0023] In this example, the detailed steps for obtaining the molecular markers that regulate the main QTL for corn ear weight are as follows:

[0024] 1. Corn F 2:3 Population Construction and Determination of Single Ear Weight

[0025] The small spike-type inbred lines Langhuang and Chang 7-2 obtained from the previous screening by our research group were used as the female parent, and the large-spike inbred line TS141 was used as the common male parent ( figure 1 ), in mid-April 2011, two F 1 The hybrid combinations are Langhuang×TS141 and Chang7-2×TS141, and two F 1 generation, let it self-cross to obtain...

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Abstract

The invention belongs to the field of crop molecular breeding, particularly relates to a molecular marker for regulating corn single spike weight main effect QTL, and also relates to a method for assisting the selection of corn single spike weight. The molecular marker for regulating the corn single spike weight main effect QTL is composed of two pairs of SSR markers of umc1120 and umc2134. The method for assisting the selection of the corn single spike weight comprises the following steps that the genome DNA of corns to be tested is extracted and PCR amplification is performed by using the umc1120 and umc2134 primers, when amplified products with the length of 338bp and 234bp are obtained, the corns to be tested are candidate large-spike-type corns. When the method is used to assist the selection of large-spike-type corn materials, only does the characteristic strip of the molecular marker need to be detected to predict the single spike weight size. The method is simple and feasible, and the selection efficiency is high. A large-spike-type corn individual plant can be identified during the corn growth early period, and the selection object is clear and not affected by the environment. A high-yield corn hybrid combination is designed accordingly.

Description

technical field [0001] The invention belongs to the field of crop molecular breeding, and specifically relates to a molecular marker for regulating the main effect QTL of corn ear weight. The invention also relates to a method for assisting selection of corn ear weight; Application method of molecular markers of major QTL in high-yield breeding of maize. [0002] technical background [0003] Corn is not only the world's largest food crop, but also an important feed, industrial raw material and energy crop, and plays an important role in ensuring global food security production. Zhang Shihuang et al. (2008) estimated that by 2020, China's maize production must be increased by 31% on the existing basis to meet the needs of the people. Therefore, high-yielding maize breeding has always been the primary breeding goal pursued by scholars and breeders. In addition, drought is one of the most important abiotic stresses that limit the safety of maize production. Generally, it can r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 彭云玲赵小强方鹏王芳
Owner 安徽英诺管理咨询有限公司
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