390results about How to "Speed up breeding" patented technology

The invention discloses a reproduction method of Chinese cymbidium seedlings, belonging to an orchid seedling reproduction technology, in particular to a reproduction technology for raising seedlings by tissue culture and mycorrhizal fungi intergrowth culture. In the reproduction method of the Chinese cymbidium seedlings, seedling acquisition mainly comprises the steps of seed germination, protocorm successive proliferation and rhizogenesis culture. The reproduction method of the Chinese cymbidium seedlings is characterized by comprising the following steps: carrying out hardening on rooted seedlings and the process of mycorhiza on mycorhiza fungi after the seedlings are obtained. By adopting the mycorhiza culture technology of sterile germination and mycorhiza fungi intergrowth of Chinese cymbidium seeds, the invention has the advantages that excellent variety can be bred, excellent Chinese cymbidium plants can be selected from the seedlings, the Chinese cymbidium breeding speed can be rapidly improved and the defects that pure tissue culture seedlings grow slowly, have long period and even do not bloom can be overcome, thus the invention has an important utilization value on large-area industrial reproduction for the Chinese cymbidium.

The invention relates to a technical method for high-efficient breeding of wheat, which comprises low-temperature vernalization, south breeding and generation adding, shuttle breeding, multi-point combined identification and the like. In particular, the technical method comprises the following steps of: treating the seeds obtained after the strong-winterness wheat blossoms for 15 to 20 days by hydrogen peroxide, washing the seeds by pure water, sprouting the seeds at the room temperature, putting the seedlings growing to 1 to 2 centimeters in an artificial weather room, performing the green-body vernalization of the seedlings for 45 and 50 days at a temperature of between 3 and 5 DEG C and a relative humidity of 70 percent and under a light intensity of 15001x; hardening the seedlings of the winter wheat undergoing the vernalization treatment at the normal temperature for 1 to 2 days, and transporting the seedlings to Yuanmou in Yunnan province; and preparing a rice seedling bed, watering and soaking the rice seedling bed, discharging visible water before transplanting, and transplanting the seedlings in mud at dusk. The disease resistance, stress resistance, adaptability and yieldstability selections of breeding materials are carried out by shuttle breeding and multi-point combined identification and the like. The technical method for breeding provides rich reserve of raw materials for the rapid improvement of the wheat variety, and also lays a sold foundation for demonstration and popularization of a new variety in large areas.

The invention discloses a winter sunlight greenhouse seedling transplantation method of potato seedling seeds. The seedling seeds collected in the same year are subjected to dormancy breaking, pregermination and the like, and cultured and transplanted in a winter. The method mainly comprises the following steps of (1) substrate preparation, (2), the dormancy breaking of the seeds, (3) the pregermination, (4) seeding, (5) seedbed management, (6) nutrition pot preparation, (7) transplantation, (8) management after the transplantation, and (9) timely harvesting. The method has the advantages that (1) the dormancy of the potato seedling seeds collected in the same year is broken effectively, so that a germination rate of the seedling seeds collected in the same year reaches above 80%; (2) the temperature and the humidity are controlled with small sheds in a sunlight greenhouse, so that chilling damages are prevented effectively; (3) enough water is sprayed before the transplantation, so that the damages to root systems of seedlings during the transplantation are reduced, and a survival rate of the transplantation of the seedlings reaches above 95%; and (4) the potato seedling seeds are collected and applied in the same year; a seed breeding period can be shortened by 1-1.5 years compared with the conventional method; and breeding, production and utilization of a new variety are accelerated.

The invention relates to an orchid germchit propagating method, belonging to an orchid seedling propagating technology, in particular to a propagating technology for seedlings cultured based on tissue culture and mycorrhiza fungi symbiosis. In the orchid germchit propagating method, the germchit can be obtained in the steps of seed germination, successive multiplication of protocorm and rooting culture. The method is characterized in that the rooted seedling can be further acclimatized and the mycorrhiza fungi are mycorrhized after the germchit is obtained by using the propagating method. By using the mycorrhized culture technology based on sterile germination and mycorrhiza fungi symbiosis of the orchid seed, fine varieties can be propagated, fine orchid plants can be selected from the seedlings of seeds, and the propagating speed of the orchid can be quickened, thereby overcoming the defects of slow growth, long cycle and even nonflowering of the pure tissue culture seedling, and having important utility values in the large-area industrialized propagation of the orchid.

The invention relates to detection primers for molecular markers in close linkage with a major QTL (Quantitative Trait Locus) of wheat ear length and an application of the detection primers. The detection primers for the molecular markers include an upstream primer, having a nucleotide sequence as shown in SEQ ID NO.1, of the molecular marker XWMC112 and a downstream primer, having a nucleotide sequence as shown in SEQ ID NO.2, of the molecular marker XWMC112, and an upstream primer, having a nucleotide sequence as shown in SEQ ID NO.3, of the molecular marker XCFD53 and a downstream primer, having a nucleotide sequence as shown in SEQ ID NO.4, of the molecular marker XCFD53. The molecular marker XWMC112 and the molecular marker XCFD53 are studied and found for the first time, and can be used for quickly determining whether a wheat variety or strain has the QTL of the ear length, and thus quickly screening out the wheat variety or strain having the QTL for increasing the ear length, and therefore, the breeding progress of a high-yield wheat variety can be accelerated.

The invention relates to a method for identifying tobacco resistance by use of a tomato spotted wilt virus NSm gene and belongs to the technical field of plant protection. The NSm gene contains a nucleotide sequence shown in SEQ ID No.1. The NSm gene is an avirulence gene which stimulates tobacco containing a resistant locus RTSW to produce disease resistance response. An agrobacterium tumefaciens transient expression system is adopted for transient expression of the NSm gene in tobacco leaves, and whether test tobacco contains the resistant locus RTSW is judged by detecting whether the test tobacco shows an allergic reaction. The resistance of the tobacco to the tomato spotted wilt virus disease can be rapidly and accurately identified with the method, and the method can be applied to disease-resistant breeding as well as positioning and cloning of disease-resistant genes.

The invention provides an artificial propagation method for Anastatus spp. which comprises the steps of, (1) producing host ovum, (2) gathering and breeding bee variety, (3) sticking cards, (4) warming the Anastatus spp., (5) loading into boxes, (6) producing ovum and parasitic operation, (7) cultivating and growing, (8) quality checking, (9) low-temperature storage, (10) warming for growth, transporting or releasing.

The invention discloses a method for rapidly obtaining a large number of wheat double-haploid homozygous populations. According to the method, based on a wheat and corn double-haploid breeding technology, through the key links of wheat and corn sowing at different stages, haploid induction, transplantation and vernalization, reduplication and transplantation and winter sowing treatment, after wheat young embryos differentiate into haploid seedlings, transplantation, vernalization and haploid plant reduplication are completed in the same year without over-summer treatment, wheat double-haploid seeds can be harvested in the same year, the requirement for sowing once more can be met after the harvest on the perspective of time, a large number of stable new strains of the wheat homozygous populations can be obtained within one year, the requirement for identifying the new strains at the next stage is met, the natural environment is utilized to the maximum degree, and the time of wheat double-haploid breeding is shortened to one year from two to three years. In this way, the wheat breeding speed is increased, the breeding efficiency is improved, the breeding cycle is shortened, and the breeding cost is lowered.

The invention provides a Brassica napus annual multi-generation generation adding method. Four stages including seed pretreatment, transplanting and seedling management, flowering phase management and pod development period management are effectively controlled, and one-year three-cropping or four-cropping (ie, annual three-generation or four-generation generation adding) of the Brassica napus is realized, the seed breeding process is effectively accelerated, and the seed breeding year limit is shortened. The controllable greenhouse cultivation technology is adopted, so that the running around at different places are avoided, the time is saved, and manpower and material resources are saved. The Brassica napus annual multi-generation generation adding method is applied to backcross or cross breeding, and seed breeding of novel high-quality and high-yield disease-resistant varieties of Brassica napus can be accelerated.

The present invention provides a process for rapidly breeding novel strains of colorful silkworm cocoons, particularly a process for breeding novel strains of natural colorful silkworm cocoons by utilizing selection of gene expression of multi-phenotype special character tags and quickening homozygosity of characters of offspring from wide cross of cultivated silkworms and wild silkworms. Based on the expressive characteristics of a plurality of appreciable morph character stigmata genes (such as ovum color, mottled grain of larvas, skin redness of larvas, volume color of scalewings, color of cocoon fibers, etc.) of offspring from wide cross of cultivated silkworms and wild silkworms at different development phases for the same generation, the wide cross color incunabulum strain syngen is constructed with the goal of precocity, easy cultivation, strong disease resistance, high yield, high feeding efficiency, thin titer and colourful cocoon fibers; the genetic amelioration of multi-phenotype tag character assistant selection and economic character orthoselection is carried out continuously for many years to stabilize the homozygosity of each system character from wide cross of silkworms; simultaneously, desired practical characters of silkworms can be sufficiently recombined and converged to obtain novel strains of natural colorful silkworm cocoons.

The invention relates to a plant tissue culture method, in particular to a method for cultivating water-saving and drought-resistant rice anther, and belongs to the field of biotechnology. According to the adopted technical scheme, the method comprises the following steps: selection of an explant, disinfection and inoculation of the explant, induction and proliferation of a callus, differentiation of the callus, and rooting, cultivating and transplanting of a tissue culture seedling. The method is applied to genetics breeding of water-saving and drought-resistant rice, improves anther culture efficiency of the water-saving and drought-resistant rice, especially indica-type water-saving and drought-resistant rice, shortens breeding cycle, and provides strong technical support for accelerating selection and breeding of fine varieties of the water-saving and drought-resistant rice.

The invention discloses a detection method for an insertion/deletion marker of a goat gene IGF2BP1. The detection method comprises the following steps: with to-be-detected goat whole-genome DNA as a template, applying a part of fragments of the goat gene IGF2BP1 by PCR, then carrying out agarose gel electrophoresis, and identifying the genotypes of the 15-bp insertion/deletion polymorphic site andthe 5-bp insertion/deletion polymorphic site of the goat gene IGF2BP1 according to electrophoresis results. According to the invention, different genotypes and a combined genotype of 15-bp and 5-bp insertion/deletion polymorphic sites of the goat gene IGF2BP1 have significant correlational relationship with the litter size character of Shaanbei white-cashmere goat, so the 15-bp and 5-bp insertion/deletion polymorphic sites can be used as DNA markers to improve the litter size character of the goat. The detection method for the insertion/deletion marker of the goat gene IGF2BP1 provided by theinvention can be applied to goat molecular marker-assisted selective breeding, and accelerates establishment of an excellent goat genetic resource population.

The invention discloses a method for breeding wheat by cold plasma treatment. The invention discloses a method for obtaining the wheat with improvement of seed germination ability, and/or reduction of plant height, and/or increasing of root length, and/or increasing of biological yield, and/or improvement of tillering ability, and/or improvement of seed production ability, which processes to-be-processed wheat raw materials by the cold plasma treatment. According to the method for breeding wheat by the cold plasma treatment, the defects of low-level repetition and serious homogeneity of traditional breeding, potential dangers of transgenic breeding, and huge economic investment of space breeding can be broken through, the breeding period can be shortened, and breeding methods and means are advanced.

The invention discloses a preparing method of protoplast of edible mushrooms and the protoplast, and relates to the technical field of preparation of engineering cells of filamentous fungi. The preparing method of the protoplast of the edible mushrooms comprises the steps of inoculating edible mushroom strains into a container containing a solid culture medium to be subjected to mycelium culture so that bacterial colonies in which hyphal is grown can be obtained; conducting cell wall enzymolysis on the bacterial colonies to obtain a protoplast coarse solution; filtering, centrifugating and re-suspending the protoplast coarse solution to obtain a protoplast solution. According to the preparing method, a large amount of protoplast can be quickly obtained. Compared with a conventional method, the preparing method of the protoplast of the edible mushrooms is greatly improved in the aspect of speeds, simple in step, and more convenient to operate.

The invention discloses a hormone-free culture medium for culturing high-quality butterfly orchid seedlings. The hormone-free culture medium comprises three culture mediums A, B and C, wherein the culture medium A consists of 1.5-2.8g/L of Huaduoduo #11, 1.5-2.5g/L of tryptone, 1.0-3.0g/L of active carbon, 20-30mg/L of glutathione, 20-30g/L of saccharose and 8.0-9.0g/L of agar powder, and the final pH of the culture medium A is adjusted to 5.2-5.5. The invention also discloses application of three culture mediums A, B and C, of the hormone-free culture medium, and used in culture of the high-quality butterfly orchid seedlings. The series of the culture mediums and a method are simple and adaptive. The conveniently applied culture is suitable for germfree sowing of selfed or interbreeded seeds of most garden seeds in conventional butterfly orchid species, so that a large quantity of high-quality seedlings of the different species of high-quality butterfly orchids can be obtained; furthermore, the aberration rate of the seedlings is reduced as no hormone is added, and the culture speed of the high-quality butterfly orchid seedlings is increased.

The invention relates to a method of braiding, planting and cultivating multiple plants of photinia fraseri into a stereoscopic landscape shape, belonging to a tree cultivation technique. The invention aims to solve the problems that the cultivation quality is influenced slightly while the cultivation success rate is severely reduced by giving up halfway and keeping all the previous effects wasted due to broken or dead plants subjected to the planting severe factors as the traditional method adopts the single-seedling planting, the production cost is high as the cultivation period is as long as 3-5 years, the optimum time to market is missed so as to cause low market price and reduced economic benefits. The method is characterized in that at least two seedlings with the height of over 30cm are braided into a bundle for planting and cultivating, and the seedlings are cultivated, pruned and braided into a bundle again for 12 months to form the stereoscopic landscape shape finished seedling according to the design requirement. After implementation, compared with the prior art, the method has the advantages that the stress resistance of the stereoscopic landscape shape is enhanced, therefore, the cultivation success rate can reach up to over 99%, the plant growth is doubled, and the cultivation time is greatly shortened to 1-2 years.

The invention discloses a detection method for goat CSN1S1 gene insertion/deletion, and applications thereof. According to the method, the whole genomic DNA of a goat to be detected is used as a template, the goat CSN1S1 gene is amplified through a PCR technology, and agarose gel electrophoresis is performed to identify whether the indel polymorphism exists in the goat CSN1S1 gene. According to the present invention, the large sample (N is more than 1000) related analysis results reveal that the different types of the CSN1S1 gene indel are significantly related to the first lambing trait of Shanbei white cashmere goat, such that the different types of the CSN1S1 gene indel can be used as the DNA mark for improving the first lambing trait of the goat; and the method for rapidly detecting the goat CSN1S1 gene indel can be used in the marker-assisted selection breeding of goat reproductive traits in China, and is conducive to the rapid establishment of the goat genetic resource groups having the high lambing number trait.

The invention discloses a detection method of goat CMTM2 gene insertion/deletion polymorphism and an application thereof. According to the invention, by using the whole genome DNA of the goat to-be-tested as a template, a partial fragment of the goat CMTM2 gene is amplified by PCR, and then agarose gel electrophoresis is carried out, According to the electrophoresis results, the genotype at a 14-bp insertion/deletion polymorphism site of the goat CMTM2 gene NC_030825.1: g.35582961_35582974 site is identified. Correlation analysis shows that the different genotypes of 14-bp insertion/deletion polymorphism of the goat CMTM2 gene and the property of litter size of Shanbei white cashmere goats have a significant correlation, and DNA markers capable of increasing the property of litter size areexisted. The method can be applied to goat molecular marker-assisted selection breeding, and accelerates the establishment of an excellent goat genetic resource group.