42results about How to "Speed up breeding" patented technology

The invention discloses a macrobrachium rosenbergii iridovirus disease resistance-related SNP (Single Nucleotide Polymorphism) site, a site combination and application thereof, and belongs to the field of aquatic organism breeding. According to the invention, SNP loci related to resistance of the macrobrachium rosenbergii iridovirus disease are successfully screened through whole genome association analysis, and the SNP loci are respectively located at a 9574545th base of a chromosome 10 of the macrobrachium rosenbergii and a 26491028th base of a chromosome 14 of the macrobrachium rosenbergii. Aiming at the two SNP sites, an SNP site combination related to the resistance of the macrobrachium rosenbergii iridovirus disease is further developed, and macrobrachium rosenbergii iridovirus disease resistant individuals can be screened by detecting the SNP site combination. Therefore, the SNP molecular marker provided by the invention can be used for macrobrachium rosenbergii molecular marker assisted breeding, accelerates disease-resistant macrobrachium rosenbergii variety breeding, and provides a reference basis for disease-resistant breeding work of macrobrachium rosenbergii.

The invention provides a molecular marker related to sheep meat quality traits and fatty acid content and application thereof, and relates to the technical field of biologication.The application comprises the step that substances for detecting SNP sites are applied to preparation of products for predicting the sheep meat quality traits and the fatty acid content. The sheep with the specific genotype at the SNP site has high meat quality character or / and high fatty acid content, and the SNP molecular marker related to the meat quality character and the fatty acid content of the sheep can be used for predicting the meat quality character and the fatty acid content of the sheep, screening sheep individuals with the high meat quality character and the high fatty acid content, and improving the sheep breeding efficiency. The invention further discloses a breeding method for obtaining sheep with high meat quality characters and high fatty acid content, and the breeding speed of dominant sheep is increased.

The invention discloses a cultivation method for promoting rapid growth and development of spring wheat, and belongs to the technical field of wheat breeding and cultivation. The cultivation method comprises the following steps: 1, seed pretreatment; 2, field planting and soil culture: transplanting wheat seeds with white buds into a seedling culture hole tray, transferring the seedling culture hole tray into a culture room with a long photoperiod, culturing until the wheat seeds are mature, and harvesting; lED light is adopted in the culture room for illumination; the LED light is blue light, white light or a mixture of red light and blue light according to a light quantum ratio of 3: 1. The method has the beneficial effects that the condition combination of regulating and controlling the light period, the light quality and the light intensity is provided. Through the combination of fixed and widely applicable illumination conditions, the method significantly shortens the growth cycle of the spring wheat. The method has the beneficial effects that the time from seed germination to mature harvesting of the spring wheat is generally 50-60 days, so that 6-7 generations of spring wheat can be harvested in one year. The wheat growth cycle is shortened, the breeding efficiency is remarkably improved, and a solid technical foundation is laid for improvement and cultivation of chassis varieties.

The invention discloses an application of an SNP molecular marker combination in height-assisted breeding of cockscombs, the SNP molecular marker combination comprises four SNP sites SNP1-SNP4, in the height-assisted breeding of the cockscombs, individuals of AA and GA dominant genotypes of the SNP1 site, CC and CT dominant genotypes of the SNP2 site, AA and GA dominant genotypes of the SNP3 site and AA and GA dominant genotypes of the SNP4 site can be selected and reserved, and the SNP molecular marker combination can be applied to the height-assisted breeding of the cockscombs. The method for eliminating other inferior genotype individuals is used for assisting in breeding for increasing the height of the cockscomb, and the progress of generation breeding of the height of the cockscomb is accelerated.

The utility model relates to a high-resistance composite fiber floating wetland which comprises a plurality of main bodies which are detachably and mutually spliced on the same plane, and each main body comprises two resistance layers which are symmetrically arranged up and down and a carrier layer which is arranged between the two resistance layers; a first sleeve and a second sleeve which are transversely arranged and vertically distributed are also inserted and fixed in the carrier layer; the height of the first sleeve is different from that of the second sleeve, so that the first sleeve and the second sleeve are staggered up and down without contact; a bonding layer is filled between the carrier layer and each of the two resistant layers; according to the utility model, the service life is longer; the floatability is effectively improved, so that the wind wave resistance is improved; in addition, the cold resistance of the aquatic plants is improved, a three-dimensional space required by breeding is provided for the aquatic plants, the breeding speed of the aquatic plants is greatly increased, the planting density of the aquatic plants is rapidly increased, and the water purification capacity is remarkably improved.

The invention discloses a method for cultivating plant-growing concrete seedlings of red sea olive, which comprises the following steps of: fixing seeds of red sea olive in a plant-growing concrete seedling-growing tube, and then directly and vertically planting the plant-growing concrete seedling-growing tube on a field mud flat wetland for natural seedling cultivation. The vegetation concrete seedling raising pipe is formed by pouring seed germination concrete on the upper layer and seedling breeding concrete on the lower layer in the seedling raising pipe in a laminated mode. Wherein the seed germination concrete and the seedling breeding concrete are prepared from sea mud, sea sand, cement, seawater and fertilizer according to different dosage ratios. According to the method, the three functions of seed germination, seedling breeding and field planting application can be achieved in the same device, and the cultivation rate, the survival rate and the retention rate of the natural seedlings of the red sea olive can be remarkably increased.

The invention relates to the technical field of intelligent management, in particular to an intelligent management system, which comprises an environmental parameter acquisition module; a towel parameter acquisition module; the data analysis module is used for constructing an environment parameter time domain curve according to the collected towel placement environment parameters, determining the cleaning frequency of the towels according to the towel placement environment parameters and calculating the time domain dispersion of the environment parameters of all collection time nodes in the environment parameter time domain curve; a self-adjusting module; a cleaning module; according to the method, the cleaning frequency for the towel is preliminarily determined according to the temperature and humidity of the environment, and the situation that the breeding speed of microorganisms on the towel is increased due to the fact that the temperature and humidity change frequency of the towel storage environment is high is considered, the variance of the environment temperature and humidity in the period is calculated; the influence of the environment on the breeding condition of microorganisms on the towel is analyzed according to the change condition of the environment temperature and humidity, and the cleaning degree of the towel is further adjusted according to the influence degree of the environment on the towel.

The invention relates to the field of genes, in particular to molecular markers for judging flowering time of brassica napus L. The molecular markers are Indel-282, Indel-326 and Indel-353 respectively and are located on an A02 chromosome of the brassica napus L.. The molecular marker has high specificity and stability, and is significantly related to the flowering time. The markers show high specificity and stability in brassica napus plants under different genetic backgrounds. By detecting the molecular markers through PCR amplification and electrophoresis, the flowering time of different plants can be quickly and accurately identified in the early stage of breeding. Therefore, the flowering time of the plant can be determined in the early stage, so that the breeding efficiency is improved. The molecular markers can be used for molecular marker-assisted selective breeding, and plants with early flowering or late flowering characteristics can be quickly screened by detecting the markers, so that the breeding process of excellent varieties is accelerated, and the functions and regulation mechanisms of related genes are further disclosed.

The invention belongs to the technical field of crop cultivation, and discloses a lettuce rapid generation adding method. The method comprises the steps that lettuce seeds are subjected to hydroponic seedling culture until each seedling has two leaves and one core, and then field planting is conducted; the nitrogen concentration is 9.0 mmol / L, the temperature is 25 DEG C, the light intensity is maintained at 200 [mu] mol.m <-2 >. S <-1 > in the first five days, the light period is 16: 8, the light quality red: blue: far-red ratio is 6: 1: 1, the light intensity is adjusted to 320 [mu] mol.m <-2 >. S <-1 > in the sixth day, the light period is 16: 8, the light quality red: blue: far-red ratio is 6: 1: 1, and culture is performed for 15 days; the cultured lettuce plants are placed in low-nitrogen nutrient liquid with the concentration of 1.5 mmol / L to be cultured, the temperature is 25 DEG C, the light intensity is 320 [mu] mol.m <-2 >. S <-1 >, the photoperiod is 20: 4, and the ratio of red light to blue light to far-red light is 6: 1: 1 until the plants bloom; after the plants bloom, adjusting the nitrogen concentration of the low-nitrogen nutrient liquid to the normal nitrogen concentration of 9.0 mmol / L until the seeds are mature. The method promotes rapid flowering and seed setting of lettuce, shortens the breeding period, and accelerates the breeding speed of lettuce.

The invention belongs to the field of molecular biology, and discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to a pig backfat thickness character and application thereof. According to the invention, an SNP (Single Nucleotide Polymorphism) site g.348Agt related to the back fat thickness character of the pig is screened in an intron region of the ADIPOQ gene; the SNP site is located at the 348bp nucleotide at the downstream of the ADIPOQ gene transcription start site, and the polymorphism of the SNP site is A or G. According to the present invention, the pig backfat thickness condition is determined according to the genotype of the mutation site, such that the functional gene and the molecular genetic marker related to the pig backfat thickness are obtained, and the dominant allele of the SNP molecular marker is optimized and the site is rapidly detected so as to determine the pig backfat thickness, reference basis can be provided for accurate evaluation of pig fat deposition capacity regulation related SNP molecular marker assisted breeding, and meanwhile, the method has important significance on pig strain or variety improvement.

The application belongs to the technical field of biology and particularly relates to a molecular marker for predicting daily weight gain of pigs, a primer pair, a kit and application thereof. The nucleotide sequence of the molecular marker is shown as SEQ ID NO. 3, and the 474th site at the 5' end of the molecular marker is a polymorphic site, the base of which is C or G, and the daily weight gain of the pig with the genotype CC of the site is higher than that of the pig with the genotype GG. The molecular marker provided in the application is a molecular marker related to the growth performance of pigs, and is used for predicting the daily weight gain, feed conversion rate and other growth traits of pigs, and provides an accurate genetic basis for early selection of pigs.

The application discloses a kind of MLANA The application discloses application of gene SNP molecular marker in black-bone chicken molecular marker assisted breeding MLANA The application discloses application of gene SNP molecular marker in black-bone chicken molecular marker assisted breeding The application discloses application of gene SNP molecular marker in black-bone chicken molecular marker assisted breeding

This invention belongs to the field of wheat breeding, specifically relating to a wheat Fusarium head blight resistance locus and molecular breeding method. This invention identified five stable Fusarium head blight resistance-associated loci, Qfhb.jaas-2D, Qfhb.jaas-3A, Qfhb.jaas-3B.1, Qfhb.jaas-5A.2, and Qfhb.jaas-6D, on chromosomes 2A, 2D, 3A, 5A, and 6D. For the identified resistance loci, this invention designed KASP molecular markers. These markers have high specificity and sensitivity, enabling accurate and rapid detection of resistance genotypes in wheat materials, providing a powerful tool for marker-assisted selection (MAS). This invention verified the effectiveness of the screened resistance loci. The results showed that the above five loci were significantly associated with Fusarium head blight resistance, providing a reliable basis for the breeding of resistant varieties.

The invention discloses a cultivation method for promoting cassava flowering, and belongs to the technical field of crop cultivation. The method comprises the following steps: (1) variety selection; (2) potted plant preparation; (3) cassava planting; (4) potted plant management; (5) first fertilization; (6) second fertilization; (7) spraying flower-inducing liquid; (8) flower preservation. The invention uses a matrix mixed with vermiculite, perlite and coarse sand to make the tubers sprout as soon as possible, and the potted plants are placed in an arch shed for cultivation to promote the growth of plants as soon as possible and advance the growth period. The phosphate fertilizer used in the first fertilization can make the stems and branches of the plants tough and promote the formation of flower buds. The potash fertilizer used in the second fertilization can promote the photosynthesis of the plants and promote the fruiting of the plants. After fertilization, sufficient water is poured to make the soil compacted so that the cassava is not easy to expand the tuberous roots. Then, the flower-inducing liquid is sprayed to make the plants bloom in advance and extend the flowering period. The invention makes the differentiation of cassava flower buds early, improves the flowering ability of cassava, and achieves the purpose of flowering and fruiting.

The invention discloses a primer pair for identifying ulmus pumila and ulmus parvifolia germplasm and application of the primer pair, the primer pair comprises the following primer pairs Ulmusp104 or / and Ulmusp40, a fresh ulmus pumila or ulmus parvifolia tissue sample to be detected is collected, and DNA of the tissue sample is extracted; carrying out PCR (Polymerase Chain Reaction) amplification by using the primer pair by taking the DNA as a template; carrying out electrophoresis detection on an amplification product; judging whether the to-be-detected sample is ulmus pumila or ulmus parvifolia according to a detection result. The SSR molecular marker for transcriptome sequencing is used for identifying ulmus pumila and ulmus parvifolia germplasm resources, so that ulmus pumila and ulmus parvifolia improved variety breeding is promoted, and the utilization process of excellent germplasm is accelerated. The specific primers are respectively used for amplifying ulmus parvifolia and ulmus pumila tissues, and the length difference of the two products is obvious, so that the specific primers can be used for effectively identifying ulmus pumila and ulmus parvifolia germplasm.

This invention belongs to the field of crop cultivation technology and discloses a method for rapid generation of lettuce. The method involves: hydroponically cultivating lettuce seeds into seedlings, which are then transplanted after reaching the stage of two leaves and one bud; the seedlings are then cultured in a hydroponic tank with a total nitrogen nutrient solution at a nitrogen concentration of 9.0 mmol / L, a temperature of 25℃, and a light intensity maintained at 200 μmol·m² for the first 5 days. ‑2 ·s ‑1 The photoperiod is 16:8, the light quality is red:blue:far-red = 6:1:1, and the light intensity is increased to 320 μmol·m⁻¹ starting from day 6. ‑2 ·s ‑1 The photoperiod was 16:8, the light quality was red:blue:far-red = 6:1:1, and the plants were cultured for 15 days. The cultured lettuce plants were then placed in a low-nitrogen nutrient solution with a nitrogen concentration of 1.5 mmol / L at 25℃ and a light intensity of 320 μmol·m⁻¹. ‑2 ·s ‑1 The photoperiod was 20:4, and the light quality was red:blue:far-red = 6:1:1. The plants were cultured until flowering. After flowering, the nitrogen concentration of the low-nitrogen nutrient solution was adjusted to the normal nitrogen concentration of 9.0 mmol / L until the seeds matured. This invention promotes rapid flowering and seed setting in lettuce, shortens the breeding cycle, and accelerates the lettuce breeding process.

The invention relates to an SNP (Single Nucleotide Polymorphism) marker for identifying the high-yield character of bee colony royal jelly of western bees and application thereof. The SNP marker is selected from NC037641.110806386 and NC037641.110806436, and the SNP marker is selected from the group consisting of NC037641.110806436 The method comprises the following steps: randomly collecting adult worker bee individuals from a to-be-detected western bee colony, extracting genome DNA as a template, and carrying out PCR amplification reaction to obtain a PCR reaction product; carrying out agarose gel electrophoresis detection on the obtained PCR reaction product, and sequencing the PCR reaction liquid with a target band; and analyzing the sequencing result, calculating the gene frequency of the NC037641.110806386 site or the NC037641.110806436 site of the western bee colony to be detected, and determining whether the western bee colony to be detected is a bee colony with high royal jelly yield or not. The two SNP markers related to the high-yield character of the bee royal jelly of the western bees are provided from the molecular biology level, and the two SNP markers comprise target fragments amplified by the same pair of specific primers, so that the high-yield character of the bee royal jelly of a bee colony of the western bees can be scientifically, accurately and quickly identified, the breeding period of the bees with high royal jelly yield can be greatly shortened, and the breeding efficiency of the bees with high royal jelly yield can be improved. The breeding speed is accelerated.

The invention relates to an efficient planting method for cut-section ginger under a rubber forest. The planting method comprises the following steps: ditching and cleaning seeds; treating and selecting rhizomes; deeply ploughing and loosening soil; performing fertilization; topdressing, intertillage and ridging and the like. According to the scheme provided by the invention, the yield of the zingiber officinale roscoe can be remarkably increased, the growth cycle of the zingiber officinale roscoe can be shortened to about 2 years, and the method has important significance on the development of plant industrialization.

The present invention discloses a detection method and application of gene polymorphic sites related to sheep meat tenderness. The genetic polymorphism of sheep CCN5 gene in a population is analyzed by sequencing technology, and correlation analysis is performed by combining with individual meat quality trait data. It is found that the sheep population with superior meat tenderness can be selected early based on the polymorphic sites on the 5th exon of the CCN5 gene.

This invention discloses a primer pair for identifying *Ulmus pumila* and *Ulmus chinensis* germplasm and its application. The primer pair is Ulmus_p104 or / and Ulmus_p40. Fresh tissue samples of *Ulmus pumila* or *Ulmus chinensis* to be tested are collected, and DNA is extracted from the tissue samples. Using this DNA as a template, PCR amplification is performed using the primer pair. The amplification products are detected by electrophoresis. Based on the detection results, the sample is determined to be either *Ulmus pumila* or *Ulmus chinensis*. This invention utilizes SSR molecular markers from transcriptome sequencing to identify *Ulmus pumila* and *Ulmus chinensis* germplasm resources, which is beneficial for promoting the breeding of superior varieties of *Ulmus pumila* and *Ulmus chinensis* and accelerating the utilization of superior germplasm. The specific primers of this invention amplify tissues of *Ulmus chinensis* and *Ulmus pumila* respectively, and the difference in product length between the two is significant, indicating that these specific primers can effectively identify *Ulmus pumila* and *Ulmus chinensis* germplasm.

This invention discloses a method for using mulching to prevent weeds in intensive flower production, relating to the field of agricultural flower technology. The mulching method includes a mulch film and a heat-insulating film. The mulch film consists of an inhibitory layer and a light-blocking layer, with the light-blocking layer adhered to the outer surface of the inhibitory layer. The heat-insulating film consists of a heat-insulating layer and a frost-proof layer, with the frost-proof layer adhered to the outer surface of the heat-insulating layer. The mulch film inhibits weed growth, ensuring that no weeds consume nutrients during the seedling and growth stages, reducing weeding costs and increasing the growth rate of flowers. The method of use involves first applying the mulch film, then flexibly cutting holes according to the plant spacing of the seedlings. This allows for flexible hole cutting based on the plant spacing, and then covering the roots with soil to prevent sunburn, reducing weeds throughout the growth period, lowering the cost of manual weeding or herbicides, and also providing warmth, moisture retention, pest control, and uniform growth.

The invention discloses a method for detecting sheep TPH2 gene mutation through HRM, and relates to the technical field of biotechnology. A high-resolution melting curve analysis (HRM) technology is adopted, and mutation is detected based on the difference of melting temperatures of double-stranded DNA in the heating denaturation process. The method comprises the following steps: designing an HRM (High Resolution Melting) primer, extracting genome DNA (Deoxyribose Nucleic Acid) in blood of to-be-detected sheep, performing amplification by using the HRM primer by taking the genome DNA as a template, heating an amplification product, obtaining a melting curve by detecting the change of a fluorescence signal, and screening out the TPH2 gene mutant in the to-be-detected sheep according to different melting curve shapes of different mutation types; the method is easy to operate, low in cost and suitable for rapid screening of known mutation sites.

The invention belongs to the technical field of apple molecular genetic breeding, and particularly relates to a gene for improving the GABA content of apples and an SNP molecular marker and application thereof. GWAS is used for analyzing and mining the regulating gene MdHVA22g related to the GABA content of the apple fruits for the first time, the gDNA sequence of the gene is shown as SEQ ID NO.1, the CDS sequence of the gene is shown as SEQ ID NO.2, a corresponding SNP molecular marker is developed through associated SNP, the SNP site is the 678th site of the gDNA of the gene, the polymorphism of the SNP site is A or C, and the GABA content of the apple fruits with the homozygous AA genotype is higher than that of the apple fruits with the homozygous CC genotype and the heterozygous AC genotype at the site. Besides, the dCAPS primer is designed by utilizing the SNP, so that the GABA contents of the apple fruits with different germplasm can be effectively and quickly distinguished, and the breeding of the high GABA variety of the apple can be accelerated.

The invention relates to an insertion and deletion marker NC037647.18674876 for identifying the high-yield character of bee colony royal jelly of western bees and application of the insertion and deletion marker NC037647.18674876 The method comprises the following steps: randomly collecting adult worker bee individuals from a to-be-detected western bee colony, extracting genome DNA (deoxyribonucleic acid) as a template, and carrying out PCR (polymerase chain reaction) amplification reaction by using a primer of NC037647.18674876 to obtain a PCR reaction product; carrying out agarose gel electrophoresis detection on the obtained PCR reaction product, and sequencing the PCR reaction liquid with a target band; nC037647.18674876 site genotyping is carried out according to a sequencing result, the genotype is a homozygous insertion type II, a homozygous deletion type DD or a heterozygous type ID, the I allele shows 14bp insertion, and the D allele shows 14bp deletion; the allele frequency of the NC037647.18674876 site of the to-be-detected western bee colony is calculated, and whether the to-be-detected western bee colony is the royal jelly high-yield bee colony or not is determined. The method can accurately, quickly and scientifically identify the royal jelly high-yield character of the western bee colony, can greatly shorten the breeding period of the royal jelly high-yield bees, and accelerates the breeding speed of the bees.

The invention belongs to the technical field of sphagnum moss planting, and particularly relates to an artificial cultivation method of sphagnum moss and application of the artificial cultivation method. The artificial cultivation method of the sphagnum moss comprises the following steps: placing a sphagnum moss stem segment growing in season in a culture container containing a nutrient solution for illumination culture to obtain artificially cultivated sphagnum moss; the nutrient solution comprises ammonium sulfate, calcium superphosphate and calcium chloride; the illumination intensity of the illumination culture is 230 to 6000 lux. The artificial cultivation method for the sphagnum moss has the advantages that the sphagnum moss is promoted to grow, the growth vigor of the sphagnum moss is good, and a direction is provided for industrial artificial cultivation of the sphagnum moss.

This invention discloses a CAPS molecular marker for enhancing the cold tolerance gene OsSGR in rice, along with its detection and identification methods and applications, belonging to the field of plant molecular breeding technology. The molecular marker consists of two primers, OsSGR-CAPS-F (SEQ ID NO.1) and OsSGR-CAPS-R (SEQ ID NO.2), and can rapidly and accurately distinguish between two OsSGR alleles through PCR amplification, Nhe I restriction enzyme digestion, and electrophoretic analysis. The presence of only a 1038 bp band indicates a cold-sensitive genotype, while the presence of two bands (231 bp and 800 bp) indicates a cold-tolerant genotype. This invention also provides a method for genotype detection using this marker and its application in backcross breeding for selecting cold-tolerant rice lines. This marker system has advantages such as accurate detection, simple operation, and high breeding efficiency, making it suitable for large-scale application.

This invention provides a SNP molecular marker associated with fatty acid content in sheep muscle and its application, relating to the field of biotechnology. Based on the discovered SNP site located in exon 1 of the SCARA5 gene, this invention not only enables rapid and accurate genotyping of the SCARA5 gene in sheep populations, but also, based on the significant correlation between this site and individual fatty acid content, allows for early screening of sheep with higher fatty acid content using SNP markers, thereby accelerating the breeding of superior sheep. Compared to traditional single-genotype selection, this invention offers higher accuracy and significant advantages in screening sheep with high fatty acid content, saving feeding costs and improving the breeding of superior sheep.

The application belongs to the field of aquatic organism breeding, and particularly relates to a rice field eel D2HGDH The application relates to a SNP site combination related to a gene and resistance to aquatic pathogenic viruses and application thereof. D2HGDH The SNP site combination related to the gene and resistance to the rice field eel rhabdovirus is located at the 149th, 365th, 394th, 902nd and 939th base from the start codon of the gene. D2HGDH The detection of the SNP site combination of the gene can screen the individuals resistant to the rhabdovirus of the rice field eel. Therefore, the SNP molecular marker can be used for molecular marker assisted breeding of the rice field eel, accelerates the breeding of the disease-resistant rice field eel, and provides a reference for the disease-resistant breeding of the rice field eel.

The present application belongs to the technical field of molecular markers and animal breeding, and particularly relates to a molecular marker related to the content of glycine, alanine and histidine in beef cattle muscle and application thereof. The present application provides a molecular marker located at the 18184950th base of chromosome 17 of a beef cattle reference genome GCF_002263795.3 version, having C / T polymorphism, which can not only realize rapid and accurate genotyping of beef cattle population SETD7 gene, but also based on the significant correlation between the site and the amino acid content of individuals, the beef cattle individuals with high amino acid content can be screened early, so as to improve the selection speed of superior beef cattle. Compared with the traditional single genotype selection, the present application has higher accuracy and significant advantages for screening beef cattle individuals with high amino acid content, saving feeding cost and improving the selection of superior beef cattle. The amino acid in the present application is one or more of glycine, alanine and histidine.

The invention discloses a vegetation concrete seedling culture device for bruguiera gymnorrhiza seedling culture and an application method thereof, and the seedling culture device is formed by pouring upper-layer seed germination concrete and lower-layer seedling breeding concrete in a same container in a laminated manner, the upper seed germination layer concrete is composed of sea mud, sea sand, cement, seawater and fertilizer as raw materials, and the lower seedling breeding concrete is composed of sea sand, cement, seawater and fertilizer as raw materials. The seedling raising device is directly and vertically planted on a field mud flat wetland, natural seedlings of bruguiera gymnorrhiza can be cultivated, the three functions of seed germination, seedling breeding and field planting application can be achieved in the same device, and the cultivation rate, the survival rate and the retention rate of the natural seedlings of bruguiera gymnorrhiza can be remarkably increased.