15results about How to "Speed up breeding" patented technology

The invention relates to the field of genes, in particular to molecular markers for judging flowering time of brassica napus L. The molecular markers are Indel-282, Indel-326 and Indel-353 respectively and are located on an A02 chromosome of the brassica napus L.. The molecular marker has high specificity and stability, and is significantly related to the flowering time. The markers show high specificity and stability in brassica napus plants under different genetic backgrounds. By detecting the molecular markers through PCR amplification and electrophoresis, the flowering time of different plants can be quickly and accurately identified in the early stage of breeding. Therefore, the flowering time of the plant can be determined in the early stage, so that the breeding efficiency is improved. The molecular markers can be used for molecular marker-assisted selective breeding, and plants with early flowering or late flowering characteristics can be quickly screened by detecting the markers, so that the breeding process of excellent varieties is accelerated, and the functions and regulation mechanisms of related genes are further disclosed.

The application belongs to the technical field of biology and particularly relates to a molecular marker for predicting daily weight gain of pigs, a primer pair, a kit and application thereof. The nucleotide sequence of the molecular marker is shown as SEQ ID NO. 3, and the 474th site at the 5' end of the molecular marker is a polymorphic site, the base of which is C or G, and the daily weight gain of the pig with the genotype CC of the site is higher than that of the pig with the genotype GG. The molecular marker provided in the application is a molecular marker related to the growth performance of pigs, and is used for predicting the daily weight gain, feed conversion rate and other growth traits of pigs, and provides an accurate genetic basis for early selection of pigs.

The application discloses a kind of MLANA The application discloses application of gene SNP molecular marker in black-bone chicken molecular marker assisted breeding MLANA The application discloses application of gene SNP molecular marker in black-bone chicken molecular marker assisted breeding The application discloses application of gene SNP molecular marker in black-bone chicken molecular marker assisted breeding

This invention belongs to the field of wheat breeding, specifically relating to a wheat Fusarium head blight resistance locus and molecular breeding method. This invention identified five stable Fusarium head blight resistance-associated loci, Qfhb.jaas-2D, Qfhb.jaas-3A, Qfhb.jaas-3B.1, Qfhb.jaas-5A.2, and Qfhb.jaas-6D, on chromosomes 2A, 2D, 3A, 5A, and 6D. For the identified resistance loci, this invention designed KASP molecular markers. These markers have high specificity and sensitivity, enabling accurate and rapid detection of resistance genotypes in wheat materials, providing a powerful tool for marker-assisted selection (MAS). This invention verified the effectiveness of the screened resistance loci. The results showed that the above five loci were significantly associated with Fusarium head blight resistance, providing a reliable basis for the breeding of resistant varieties.

This invention belongs to the field of crop cultivation technology and discloses a method for rapid generation of lettuce. The method involves: hydroponically cultivating lettuce seeds into seedlings, which are then transplanted after reaching the stage of two leaves and one bud; the seedlings are then cultured in a hydroponic tank with a total nitrogen nutrient solution at a nitrogen concentration of 9.0 mmol / L, a temperature of 25℃, and a light intensity maintained at 200 μmol·m² for the first 5 days. ‑2 ·s ‑1 The photoperiod is 16:8, the light quality is red:blue:far-red = 6:1:1, and the light intensity is increased to 320 μmol·m⁻¹ starting from day 6. ‑2 ·s ‑1 The photoperiod was 16:8, the light quality was red:blue:far-red = 6:1:1, and the plants were cultured for 15 days. The cultured lettuce plants were then placed in a low-nitrogen nutrient solution with a nitrogen concentration of 1.5 mmol / L at 25℃ and a light intensity of 320 μmol·m⁻¹. ‑2 ·s ‑1 The photoperiod was 20:4, and the light quality was red:blue:far-red = 6:1:1. The plants were cultured until flowering. After flowering, the nitrogen concentration of the low-nitrogen nutrient solution was adjusted to the normal nitrogen concentration of 9.0 mmol / L until the seeds matured. This invention promotes rapid flowering and seed setting in lettuce, shortens the breeding cycle, and accelerates the lettuce breeding process.

This invention discloses a primer pair for identifying *Ulmus pumila* and *Ulmus chinensis* germplasm and its application. The primer pair is Ulmus_p104 or / and Ulmus_p40. Fresh tissue samples of *Ulmus pumila* or *Ulmus chinensis* to be tested are collected, and DNA is extracted from the tissue samples. Using this DNA as a template, PCR amplification is performed using the primer pair. The amplification products are detected by electrophoresis. Based on the detection results, the sample is determined to be either *Ulmus pumila* or *Ulmus chinensis*. This invention utilizes SSR molecular markers from transcriptome sequencing to identify *Ulmus pumila* and *Ulmus chinensis* germplasm resources, which is beneficial for promoting the breeding of superior varieties of *Ulmus pumila* and *Ulmus chinensis* and accelerating the utilization of superior germplasm. The specific primers of this invention amplify tissues of *Ulmus chinensis* and *Ulmus pumila* respectively, and the difference in product length between the two is significant, indicating that these specific primers can effectively identify *Ulmus pumila* and *Ulmus chinensis* germplasm.

This invention discloses a CAPS molecular marker for enhancing the cold tolerance gene OsSGR in rice, along with its detection and identification methods and applications, belonging to the field of plant molecular breeding technology. The molecular marker consists of two primers, OsSGR-CAPS-F (SEQ ID NO.1) and OsSGR-CAPS-R (SEQ ID NO.2), and can rapidly and accurately distinguish between two OsSGR alleles through PCR amplification, Nhe I restriction enzyme digestion, and electrophoretic analysis. The presence of only a 1038 bp band indicates a cold-sensitive genotype, while the presence of two bands (231 bp and 800 bp) indicates a cold-tolerant genotype. This invention also provides a method for genotype detection using this marker and its application in backcross breeding for selecting cold-tolerant rice lines. This marker system has advantages such as accurate detection, simple operation, and high breeding efficiency, making it suitable for large-scale application.

This invention provides a SNP molecular marker associated with fatty acid content in sheep muscle and its application, relating to the field of biotechnology. Based on the discovered SNP site located in exon 1 of the SCARA5 gene, this invention not only enables rapid and accurate genotyping of the SCARA5 gene in sheep populations, but also, based on the significant correlation between this site and individual fatty acid content, allows for early screening of sheep with higher fatty acid content using SNP markers, thereby accelerating the breeding of superior sheep. Compared to traditional single-genotype selection, this invention offers higher accuracy and significant advantages in screening sheep with high fatty acid content, saving feeding costs and improving the breeding of superior sheep.

The application belongs to the field of aquatic organism breeding, and particularly relates to a rice field eel D2HGDH The application relates to a SNP site combination related to a gene and resistance to aquatic pathogenic viruses and application thereof. D2HGDH The SNP site combination related to the gene and resistance to the rice field eel rhabdovirus is located at the 149th, 365th, 394th, 902nd and 939th base from the start codon of the gene. D2HGDH The detection of the SNP site combination of the gene can screen the individuals resistant to the rhabdovirus of the rice field eel. Therefore, the SNP molecular marker can be used for molecular marker assisted breeding of the rice field eel, accelerates the breeding of the disease-resistant rice field eel, and provides a reference for the disease-resistant breeding of the rice field eel.

The present application belongs to the technical field of molecular markers and animal breeding, and particularly relates to a molecular marker related to the content of glycine, alanine and histidine in beef cattle muscle and application thereof. The present application provides a molecular marker located at the 18184950th base of chromosome 17 of a beef cattle reference genome GCF_002263795.3 version, having C / T polymorphism, which can not only realize rapid and accurate genotyping of beef cattle population SETD7 gene, but also based on the significant correlation between the site and the amino acid content of individuals, the beef cattle individuals with high amino acid content can be screened early, so as to improve the selection speed of superior beef cattle. Compared with the traditional single genotype selection, the present application has higher accuracy and significant advantages for screening beef cattle individuals with high amino acid content, saving feeding cost and improving the selection of superior beef cattle. The amino acid in the present application is one or more of glycine, alanine and histidine.

This invention relates to the field of molecular biology, specifically to SNP molecular markers associated with palmitoleic acid trait in sheep muscle and their applications. Based on the detection of exons in the sheep SMAD1 gene, this invention identifies an SNP site at position 493 of exon 2 of the SMAD1 gene or position 13,440,579 of chromosome 17 of sheep. The base at this site is either A or G, and allele A is positively correlated with high palmitoleic acid trait in muscle. Furthermore, a method for detecting this SNP molecular marker is provided to achieve rapid and accurate genotyping of the SMAD1 gene in sheep populations (e.g., double-dried meat sheep). Moreover, based on the significant correlation between this site and individual palmitoleic acid and fatty acid content, SNP markers can be used to screen sheep individuals with higher palmitoleic acid content at an early stage, thereby improving the speed of breeding superior sheep.

The application discloses a haplotype molecular marker related to cotton fiber quality traits and application thereof, and relates to the technical field of molecular detection.The haplotype molecular marker is any one of (1)-(3): (1) a haplotype molecular marker with a nucleotide sequence as shown in SEQ ID NO. 1; (2) a haplotype molecular marker with a nucleotide sequence as shown in SEQ ID NO. 2; (3) a haplotype molecular marker with a nucleotide sequence as shown in SEQ ID NO. 3.The haplotype molecular marker can assist in identifying the advantages and disadvantages of cotton fiber length, fiber strength and micronaire value.The haplotype molecular marker has very great application value for identifying comprehensive excellent traits of cotton, and has important significance for accelerating the breeding of new cotton varieties with high quality and improving the economic benefits of cotton.

The invention discloses a molecular marker closely linked with a rape pod length character and application of the molecular marker, and relates to the field of plant molecular biology. The molecular marker provided by the invention is C4-ID28, and comprises a forward primer and a reverse primer of which the nucleotide sequences are respectively shown as SEQ ID NO.1 and SEQ ID NO.2. Through identification, the primer of the molecular marker can be used for genotype identification in the early stage of breeding, and when the amplification result of the primer is a single electrophoretic band, the single plant is judged to be in a short-silique phenotype; and if the amplification result is two electrophoretic bands, determining that the single plant is in a long-silique phenotype. The primer can accurately screen the silique length phenotype, significantly improve the selection efficiency and reduce the field workload, thereby accelerating the breeding process.

The application belongs to the technical field of plant breeding, and particularly relates to a seed pretreatment method and seedling raising method for improving the breeding efficiency of Carpinus putolekii, which comprises the following steps: disinfecting Carpinus putolekii seeds, and performing temperature-variable stratification treatment on the disinfected seeds to obtain pretreated Carpinus putolekii seeds; the temperature-variable stratification treatment comprises: 23-27 DEG C warm treatment for 30 days, and 3-5 DEG C cold treatment for 60 days. The pretreatment method can significantly shorten the germination time of Carpinus putolekii, improve the germination efficiency, and provide technical support for the protection and large-scale seedling raising of the species. The results of the examples show that, by using the method provided in the application, the germination time of Carpinus putolekii seeds is reduced to 14 days, the germination rate is 93.1%, and the seedling rate is 89.7%, so the germination is rapid, and the uniformity is high.

This invention belongs to the field of aquatic organism breeding, specifically relating to grass carp. TLR1 This invention relates to SNP sites, site combinations, and applications of genes associated with resistance to aquatic pathogens. Through PCR amplification sequencing and PCR-RFLP association analysis, this invention successfully screened grass carp for resistance. TLR1 The SNP loci associated with Aeromonas hydrophila resistance are located at bases 21444058 and 21444916 on chromosome 14, respectively. Further combinations of SNP loci associated with Aeromonas hydrophila resistance were developed targeting these two SNP loci, and these combinations were applied to grass carp. TLR1 The detection of SNP locus combinations can screen grass carp for individuals resistant to Aeromonas hydrophila. Therefore, the SNP molecular markers provided by this invention can be used for marker-assisted breeding of grass carp, accelerating the selection of disease-resistant grass carp varieties and providing a reference for disease-resistant grass carp breeding.