Reproduction method of Chinese cymbidium seedlings

Abstract

The invention discloses a reproduction method of Chinese cymbidium seedlings, belonging to an orchid seedling reproduction technology, in particular to a reproduction technology for raising seedlings by tissue culture and mycorrhizal fungi intergrowth culture. In the reproduction method of the Chinese cymbidium seedlings, seedling acquisition mainly comprises the steps of seed germination, protocorm successive proliferation and rhizogenesis culture. The reproduction method of the Chinese cymbidium seedlings is characterized by comprising the following steps: carrying out hardening on rooted seedlings and the process of mycorhiza on mycorhiza fungi after the seedlings are obtained. By adopting the mycorhiza culture technology of sterile germination and mycorhiza fungi intergrowth of Chinese cymbidium seeds, the invention has the advantages that excellent variety can be bred, excellent Chinese cymbidium plants can be selected from the seedlings, the Chinese cymbidium breeding speed can be rapidly improved and the defects that pure tissue culture seedlings grow slowly, have long period and even do not bloom can be overcome, thus the invention has an important utilization value on large-area industrial reproduction for the Chinese cymbidium.

Description

technical field

[0001] The invention belongs to a technique for growing orchid seedlings, in particular to a technique for growing seedlings through symbiosis of tissue culture and mycorrhizal fungi. Background technique

[0002] Orchid refers to the general term of the whole Orchidaceae. In my country, the traditional concept of "orchid" only refers to the Orchidaceae and Cymbidium plants produced in my country. Orchidaceae is a class of precious plants with high ornamental value and economic value. Among them, there are 100 genera and more than 530 species of orchids in Yunnan, ranking first in the country.

[0003] But so far, the development and utilization of orchids in my country are still in the primary stage, and the problem of mass reproduction has not been solved, so the contradiction between supply and demand is prominent. On the one hand, driven by economic interests, farmers went to the mountains to dig a large number of wild orchids, which severely damaged w...