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Reproduction method of Chinese cymbidium seedlings

A technology for seedlings and orchids, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as the inability of orchids to meet the protection of orchid plant resources, shorten the breeding cycle, overcome slow growth, and improve breeding. effect of speed

Inactive Publication Date: 2011-03-09
SOUTHWEST FORESTRY UNIVERSITY
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AI Technical Summary

Problems solved by technology

[0005] What the present invention intends to solve is the problem that the orchid plant resource protection in my country cannot be satisfied only by ramets and tissue culture propagation.

Method used

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Examples

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Embodiment 1

[0016] Embodiment 1: select for use in 2003 in Xishuangbanna, Yunnan Province, the wild good individual plant of the Molan seeds that are gathered, in June, 2004, carry out hardening in the greenhouse of Southwest Forestry College, this breeding method Molan seedling survival rate Reached more than 95%, the specific seedling propagation process is as follows:

[0017] (1) Seed germination: the capsules matured in the field for 180 days were harvested, planted in a 4-degree refrigerator and refrigerated for 2 weeks, washed with running water for 20 minutes, and the uncracked capsules of Molan were taken as explants. Disinfect the surface in 70-75% alcohol for 30-50 seconds, rinse with sterile water once, then disinfect with 2% sodium hypochlorite for 20 minutes, rinse with sterile water for 4-5 times, and then disinfect with 0.1% mercury chloride solution 10 to 15 minutes, and finally rinsed with sterile water 5 times. Put the washed mature capsules on sterilized filter paper ...

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Abstract

The invention discloses a reproduction method of Chinese cymbidium seedlings, belonging to an orchid seedling reproduction technology, in particular to a reproduction technology for raising seedlings by tissue culture and mycorrhizal fungi intergrowth culture. In the reproduction method of the Chinese cymbidium seedlings, seedling acquisition mainly comprises the steps of seed germination, protocorm successive proliferation and rhizogenesis culture. The reproduction method of the Chinese cymbidium seedlings is characterized by comprising the following steps: carrying out hardening on rooted seedlings and the process of mycorhiza on mycorhiza fungi after the seedlings are obtained. By adopting the mycorhiza culture technology of sterile germination and mycorhiza fungi intergrowth of Chinese cymbidium seeds, the invention has the advantages that excellent variety can be bred, excellent Chinese cymbidium plants can be selected from the seedlings, the Chinese cymbidium breeding speed can be rapidly improved and the defects that pure tissue culture seedlings grow slowly, have long period and even do not bloom can be overcome, thus the invention has an important utilization value on large-area industrial reproduction for the Chinese cymbidium.

Description

technical field [0001] The invention belongs to a technique for growing orchid seedlings, in particular to a technique for growing seedlings through symbiosis of tissue culture and mycorrhizal fungi. Background technique [0002] Orchid refers to the general term of the whole Orchidaceae. In my country, the traditional concept of "orchid" only refers to the Orchidaceae and Cymbidium plants produced in my country. Orchidaceae is a class of precious plants with high ornamental value and economic value. Among them, there are 100 genera and more than 530 species of orchids in Yunnan, ranking first in the country. [0003] But so far, the development and utilization of orchids in my country are still in the primary stage, and the problem of mass reproduction has not been solved, so the contradiction between supply and demand is prominent. On the one hand, driven by economic interests, farmers went to the mountains to dig a large number of wild orchids, which severely damaged w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H17/00A01G1/00
Inventor 伍建榕马焕成丁晖胡隽王锦张东华洪英娣刘丽
Owner SOUTHWEST FORESTRY UNIVERSITY
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