86results about "Symbiotic/parasitic combinations" patented technology

The invention provides methods for preparing a conifer seedling with increased tolerance to a pest. A conifer seedling is inoculated with an isolated endophyte when the conifer seedling is susceptible to colonization by the endophyte.

This invention relates to methods and materials for modulating seed nutritional quality traits of seeds produced by a wheat, cotton, soybean, or maize plant, said plant having been heterologously disposed to, or grown from, a plant element treated with an endophyte.

Plant propagation material treated with a carbonaceous material is provided. Such treated plant propagation material is useful in a method of inducing microbial interaction with the plant propagation material that confers a benefit on the plant propagation material. The method includes placing the treated plant propagation material in a growth medium containing endogenous soil microorganisms.

The invention discloses a reproduction method of Chinese cymbidium seedlings, belonging to an orchid seedling reproduction technology, in particular to a reproduction technology for raising seedlings by tissue culture and mycorrhizal fungi intergrowth culture. In the reproduction method of the Chinese cymbidium seedlings, seedling acquisition mainly comprises the steps of seed germination, protocorm successive proliferation and rhizogenesis culture. The reproduction method of the Chinese cymbidium seedlings is characterized by comprising the following steps: carrying out hardening on rooted seedlings and the process of mycorhiza on mycorhiza fungi after the seedlings are obtained. By adopting the mycorhiza culture technology of sterile germination and mycorhiza fungi intergrowth of Chinese cymbidium seeds, the invention has the advantages that excellent variety can be bred, excellent Chinese cymbidium plants can be selected from the seedlings, the Chinese cymbidium breeding speed can be rapidly improved and the defects that pure tissue culture seedlings grow slowly, have long period and even do not bloom can be overcome, thus the invention has an important utilization value on large-area industrial reproduction for the Chinese cymbidium.

The present invention relates to Glomus mosseae 93-6 as one kind of efficient drought-resisting and high phosphate-tolerant nutrients bush mycorrhizal fungus and its production process. The Glomus mosseae preparation is prepared with kaoliang as host plant, mixture of zeolite and sand as culture medium. The dried preparation contains 40-65 spores in each ml. Glomus mosseae may be grown in various kinds of plants to improve nutrients absorption, raise drought resistance and promote growth of plant. Test shows that the present invention has excellent seedling strengthening and yield increasing effect on straw berry, rice, water melon and other crops.

The invention discloses a strain for promoting germination and seedling formation of a Dendrobium devonianum seed and application thereof. The classification designation of the strain is Tulasnella sp.CY collected to China General Microbiological Culture Collection Center, wherein the collection number is CGMCC NO.9551. The strain can obviously promote germination and seedling formation of the Dendrobium devonianum seed, and has higher specificity. The strain has very import practical meanings for acquiring the symbiotic seed and seedling of Dendrobium devonianum through seed, can be used for nursery seedling raising or outdoor trunk seed direct sowing, has the advantages of low cost, high outdoor survival rate, no tissue degradation and the like as compared with tissue culture, has the application value of acquiring the symbiotic seed and seedling instead of tissue culture, and provides reliable technical guarantees for solving the bottleneck problem of seed and seedling sources in the Dendrobium devonianum culture industry.

The present invention relates to an application technique of mycorrhizal fungi in cultivation of anoectochilus formosanus. It is characterized by that the used mycena-generic four fungi respectively are mycena dendrobii, mycena osmundicola, mycena orchidicola and mycena anoectochila. First, the mycorrhizal fungi and seedling of anoectochilus formosanus are respectively cultured, then they are symbiotically cultivated. The adoption of said invented technique can greatly shorten the growth period of anoectochilus formosanus, and can raise its yield and effective component content.

Disclosed is a process for comprehensive treatment of discarded metal tailings, wherein fungus root technology is employed to recover the metal tailing vegetation, the process comprises the steps of, (1) preparing the seeding agent, (2) preparing the basic plant material, (3) carrying out seeding bacteriorhiza treatment to plants by using the seeding agent obtained in (1), (4) conducting physical and chemical analysis to the metal tailing and related treatment, (5) sowing seeds or replanting young sprouts on the skin layer of the treated metal tailing, (6) curing the young sprouts.

The present invention is seed bed for culturing mycorrhizic seedling and its obtaining method and culture method. The seed bed for culture exogenous mycorrhizic edible fungi and medicinal fungi has medium of soil and / or artificial medium without competitive foreign fungi. The seed bed has competitive foreign fungi eliminated via sterilization. The present invention provides condition for large scale culture of mycorrhizic fungi in low cost.

The invention relates to a method for plant restoring soil of arsenic contamination, which is realized through inoculating arbuscular mycorrhizal fungi to centipedefern, a kind of pteridophyte, thus producing fungus root, the method comprises the steps of, (1) collecting raw pteridophyte material, obtaining spores of pteridophytes, cultivating young plant sprout, (2) producing fungus roots from pteridophyte, (3) transplanting the adult strain of young sprouts to arsenic contaminated soil. The invention can be applied to aid the arsenic super rich centipedefern to adapt to arsenic contamination environment.

The present invention relates to new methods of selecting and breeding organisms, in particular organisms which exhibit symbiotic behaviour with symbionts, and to new organisms and symbiota developed thereby, such as plant or grass and endophyte symbiota. Multiple symbionts are deployed in multiple organisms and selected for improved symbiotic compatibility and performance early in the breeding process. Methods include producing improved organisms from germplasm, by inoculating host organism germplasm libraries with symbionts selected from symbiont libraries and selecting improved host organisms exhibiting desired symbiota characteristics, and selection of organism-symbiont associations with a desired genetic and metabolic profile by metagenomic analysis of nucleic acid libraries from an organism or organism-symbiont association.

An intercrop method for cultivating cistanche and ephedra includes such steps as planting shelter forest including necessary tree and the host o f cistanche, and alternatively planting the host of cistanche and ephedra in south-north ridges. Its advantages are controlling desert and high economic benefit.

The invention concerns a method for the phytoremediation treatment of a medium contaminated with at least one element selected from the group consisting of (preferably water soluble and volatile) organic pollutants, heavy metals, radionuclides or a mixture thereof, comprising the step of cultivating upon the contaminated medium a plant associated with an endophytic microorganism able to improve the phytoremediation of the plant, to reduce phytotoxicity of chemicals. The invention further relates to methods for improving phytoremediation by directly modifying members of the endogenous endophytic community of a plant, via horizontal gene transfer. Another part of the invention relates to plants associated with such endophytes and / or plants with a modified endogenous endophytic community.

The present invention relates to an application technique of the mycorrhizal fungi in cultivatino of dendrobium. It is characterized by that the used mycena-generic four fungi respectively are mycenadendrobii, mycena osmundicola, mycena orchidicola and mycena anoectochila. Firstly, the mycorrhizal fungi and dendrobium seedling are respectively cultured, then they are symbiotically cultivated. The adoption of said invented technique can greatly shorten the growth period of dendrobium, and can raise yield of dendrobium and its effective component content.

The invention relates to a preparation method for an Ophiocordyceps sinensis strain material and a method for infecting Thitarodes larvae with the Ophiocordyceps sinensis strain material. According to the methods, the principle that the imperfect stage of Ophiocordyceps sinensis--Hirsutella sinensis and plant tissue form a symbiont is employed; a prepared Ophiocordyceps sinensis strain source is inoculated onto the plant tissue; proper conditions are created to promote symbiosis of the Hirsutella sinensis and the plant tissue so as to activate infection activity of the Hirsutella sinensis on the host Thitarodes larvae; the activated Ophiocordyceps sinensis strain Hirsutella sinensis forms the fungus-plant symbiont with the plant tissue; and the fungus-plant symbiont is used as the strain material with high infection activity on the host Thitarodes larvae. The infection rate is up to 85.6 to 98.9% by directly feeding the Thitarodes larvae with the prepared strain material for infection.

The present invention relates to the biosynthesis of indole diterpene compounds. In particular, the invention relates to genes encoding enzymes considered responsible for the synthesis of lolitrems.

This invention relates generally to the field of compositions and methods for developing biofertilizers of organic origin and mycorrhizal origin in particular. The invention focuses on the isolation and characterization of the various formulations and ensuing compositions developed thereof from the arbuscular mycorrhizal fungal propagules whose benefit in crop productivity is well known. The invention more particularly describes the isolation and characterization, including but not confined to, novel mycorrhizae-based biofertilizer compositions and biofertilizer formulations for use in soil fertilization and reclamation of industrially created wastelands.

This invention relates to methods and compositions for improving plant health. The present invention includes methods for making an enriched library of treatments capable of improving plant health, methods for an making an enriched library of plants capable of being improved by a treatment, and methods of marketing a plant treatment, as well as synthetic compositions comprising treatments produced by the methods of the present invention, synthetic compositions comprising endophytes capable of improving plant health, and nucleic acid probes and nucleic acid detection kits that may be used in the methods of the present invention.

The present invention relates to joint nitrogen-fixing plant symbiosis technology and is the constructing method of nitrogen-fixing plants. The present invention combines the observation and test in pure culture condition and large scale field simulation. The specific operations include the selection of fungus and bacterium variety; and the combination of mycorhiza fungi, nitrogen fixing, bacteria and symbiotic nitrogen-fixing plants to construct symbiont. The overwhelming combination includes VA mycorhiza fungi, exogenous mycorhiza fungi and nitrogen fixing bacteria separated from root of nitrogen-fixing tree. The said method can utilize the superiority of both nitrogen-fixing plant and microbes to restore the ecological environment of fragile zone.

The sexual propagation and synchronously cultivating for gastrodia tuber includes the following steps: setting honey agaric inside hole cut in wood for germination; mixing germinated honey agaric and gastrodia tuber seed; spreading 5-10 cm thick sandy soil to selected land; laying wetted tree leaf; seeding with the mixed gastrodia tuber seed; laying the wood; filling branches in wood gaps; spreadying honey agaric onto the branches and covering 5 cm thick sandy soil. The said process can make gastrodia tuber seed germinate into corm and grow into small gastrodia tuber.

Glomus iranicum var. tenuihypharum var. nov. strain deposited under BCCM deposit number 54871, comprising the sequence identified by SEQ ID NO: 1; composition comprising said strain and 2:1 smectite clays and use thereof as bio-stimulant. The invention also discloses a composition comprising said strain, fungicides, bio-fungicides, insecticides, bio-insecticides, nematicides and bio-nematicides.

The present invention relates to a method for colonizing a root-organ from a plant with a mycorrhizal fungus that is representative of the naturally process of colonization of plant roots by mycorrhizal fungi. The present invention also relates to the use of this method for studying in vitro the colonization of plant roots with a mycorrhizal fungus and to an in vitro model for studying the colonization of plant roots with a mycorrhizal fungus.