Strain for promoting germination and seedling formation of Dendrobium devonianum seed and application thereof

A technology of Dendrobium dendrobium and bacterial strains, which is applied in the field of fungal strains of the genus Colloidal, can solve the problems of being unsuitable for large-scale industrial development, low seedling reproduction coefficient, and reduced seedling quality, and achieves the problems of low cost and unorganized degradation , the effect of promoting germination

A technology of Dendrobium dendrobium and bacterial strains, which is applied in the field of fungal strains of the genus Colloidal, can solve the problems of being unsuitable for large-scale industrial development, low seedling reproduction coefficient, and reduced seedling quality, and achieves the problems of low cost and unorganized degradation , the effect of promoting germination

CN104651239AActive Publication Date: 2015-05-27JINGHONG HONGZHEN AGRI TECH CO LTD
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  • Strain for promoting germination and seedling formation of Dendrobium devonianum seed and application thereof
  • Strain for promoting germination and seedling formation of Dendrobium devonianum seed and application thereof
  • Strain for promoting germination and seedling formation of Dendrobium devonianum seed and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0024] Embodiment I, the isolation and identification of Tulasnella sp.CY bacterial strain

[0025] 1. Isolation of Glycobacteria CY strain

[0026] 1. Acquisition of protocorms: Collect the naturally germinated protocorms of Dendrobium dendrobium near the adult plants of Dendrobium dendrobium tea trees in Longpa ancient tea garden, Jinuo Township, Jinghong City, Xishuangbanna Dai Autonomous Prefecture, and use the moist moss grown on the tea tree trunks Wrapped and brought back to the laboratory for follow-up experiments.

[0027] 2. Induction, separation, purification and preservation of symbiotic fungi in protocorms: Take out the germinated protocorms, put them into a sterilized beaker filled with 1% sodium hypochlorite (NaClO) solution, shake the beaker gently, and use a sterilized blunt tip after 5 minutes Take out the tweezers and rinse with sterile water 3 to 4 times. On the ultra-clean workbench, cut the protocorm in half with a sterile blade, and place it in PDA med...

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Abstract

The invention discloses a strain for promoting germination and seedling formation of a Dendrobium devonianum seed and application thereof. The classification designation of the strain is Tulasnella sp.CY collected to China General Microbiological Culture Collection Center, wherein the collection number is CGMCC NO.9551. The strain can obviously promote germination and seedling formation of the Dendrobium devonianum seed, and has higher specificity. The strain has very import practical meanings for acquiring the symbiotic seed and seedling of Dendrobium devonianum through seed, can be used for nursery seedling raising or outdoor trunk seed direct sowing, has the advantages of low cost, high outdoor survival rate, no tissue degradation and the like as compared with tissue culture, has the application value of acquiring the symbiotic seed and seedling instead of tissue culture, and provides reliable technical guarantees for solving the bottleneck problem of seed and seedling sources in the Dendrobium devonianum culture industry.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and in particular relates to a fungus strain of the genus Colloidal which can effectively promote the symbiotic germination of dendrobium dendrobium seeds to form protocorms, and grow and develop a large number of seedlings. At the same time, the invention also relates to the specific application of the bacterial strain in the breeding of Dendrobium dendrobium. Background technique [0002] As a traditional Chinese medicinal material, Dendrobium devonianum has extremely high medicinal value, and its cultivation scale has developed into the second pillar industry after Dendrobium candidum in some prefectures of Yunnan. There are roughly four sources of seedlings used in the industry: the first is wild plants, which were mainly seen in some small individual enterprises in the 1990s. With the scarcity of wild resources, this practice cannot continue. For the protection of plants, it is ill...

Claims

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Application Information

Patent Timeline
27 May 2015
Publication
CN104651239A
IPC
C12N1/14; A01H17/00; C12R1/645
CPC
A01H17/00; C12N1/14; C12N1/145; C12R2001/645; Y02A40/10
Inventors
高江云; 邵士成