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Application technology of mycorrhizal fungi in Anoectochilus blume cultivation

A mycorrhizal fungus, applied technology, applied in application, botany equipment and methods, horticulture, etc., can solve the problems of long cycle, low content of active ingredients, slow cultivation and growth, etc., and achieve the effect of increasing alkaloid content

Inactive Publication Date: 2003-08-06
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a technology for the application of mycorrhizal fungi in the cultivation of Aureus aureus, which can solve the problems of low reproductive rate, slow and long cultivation period, low content of active ingredients and poor efficacy of Aureus aureus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. The cultivation of dendrobium fungus

[0031] (1) After activating the strains of the slanted test tubes of Dendrobium nobile bacteria once stored at low temperature, they were transferred to PDA plates respectively, and when cultured at a constant temperature of 25°C for 12-20 days, the edge of the colonies in the plates were respectively punched with a φ8mm hole puncher. Bacteria were punched into flakes, and small fragments were inserted into the following liquid medium for cultivation.

[0032] (2) Preparation of liquid medium: glucose 2.5%; KH 2 PO 4 0.15%;K 2 HPO 4 0.1%; MgSO 4 0.15%; natural products: wheat bran 3% (cooking juice).

[0033] (3) Culture conditions: after autoclaving, insert the above-mentioned fungi respectively, and its culture conditions: culture medium pH5.6; Erlenmeyer flask shaker culture bottle capacity 50%; rotating speed 120 rev / min; temperature: 23 ℃; cultivate for 30 days reward. The inoculum size of the fermenter was 10%, a...

Embodiment 2

[0049] The operating conditions of this embodiment are basically the same as in Embodiment 1, the difference is:

[0050] 1. The mycorrhizal fungi used in the present embodiment are Dendrobium chrysalis, Osmanthus osmanthus, Orchid chrysanthemum and Osmanthus edulis.

[0051] 2. Adopt solid medium, the preparation of this medium: broad-leaved tree sawdust:wheat bran:corn grain=1:1:0.5, the amount of water added is 200% of the raw material. After autoclaving, the above four fungi were inoculated respectively. When mycelium grows from the top of the culture medium to the bottom (about 1.5 months), take it out for later use.

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Abstract

The present invention relates to an application technique of mycorrhizal fungi in cultivation of anoectochilus formosanus. It is characterized by that the used mycena-generic four fungi respectively are mycena dendrobii, mycena osmundicola, mycena orchidicola and mycena anoectochila. First, the mycorrhizal fungi and seedling of anoectochilus formosanus are respectively cultured, then they are symbiotically cultivated. The adoption of said invented technique can greatly shorten the growth period of anoectochilus formosanus, and can raise its yield and effective component content.

Description

technical field [0001] The present invention relates to a kind of mycorrhizal cultivation technique of Auranthus aureus, in particular to an application technology of mycorrhizal fungus in the cultivation of Auranthus aureus, which includes mycorrhizal fungus used in the present invention, cultivation of mycorrhizal fungus, golden thread The optimal medium for lotus bud proliferation and differentiation, cluster bud formation and seedling subculture, the application technology of mycorrhizal fungi in the cultivation of A. Impact. Background technique [0002] Anoectochilus roxburghii (Wall.) Lindl.] is a plant of the family Orchidaceae, which is a rare Chinese herbal medicine. It belongs to Lu Shenglan, and the whole plant can be used as medicine. It tastes sweet and slightly bitter, and has a flat and slightly cold nature. Folks are mostly used for anti-inflammatory, analgesic, and treatment of tuberculosis, lung-heat cough, rheumatoid arthritis, convulsions in children, ...

Claims

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Application Information

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IPC IPC(8): A01G1/04A01G7/00A01H4/00A01H17/00
Inventor 郭顺星于雪梅高微微肖培根
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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