The invention discloses a preparation method of 
starch-free enzymolysis 
corn flour. The preparation method comprises the following steps: pre-crushing, cooking, defibrination, enzymolysis, 
filtration, concentration, homogenization, sterilization, 
spray drying, and packaging. The preparation method provided by the invention specifically comprises the steps of pre-crushing corn grains firstly, then putting the crushed corn grains into a cooking kettle for preliminary cooking, then adding 
sodium tripolyphosphate and 
sodium citrate, and passing the mixture through a 
colloid mill; and adding medium-temperature alpha-
amylase into an enzymolysis tank for enzymolysis, lowering the temperature, adding 
cellulase for enzymolysis, adding 
sodium D-isoascorbate and 
sodium bicarbonate after enzymolysis, performing 
filtration, concentration, homogenization and sterilization, and finally, performing 
spray drying to prepare the enzymolysis 
corn flour. The 
starch in corns is thoroughly removed from the enzymolysis 
corn flour prepared by the method, the 
mouthfeel is significantly improved, and the phenomenon of 
retrogradation agglomeration in a later period of a corn beverage can also be solved.