10 results about "Aflatoxin contamination" patented technology

The invention discloses an aflatoxin degrading enzyme mutant and application thereof, and belongs to the technical field of enzyme engineering and microorganisms. In order to solve the technical problems that an existing aflatoxin B1 degrading enzyme mostly depends on a mild operation environment, the degradation performance is poor in a high-temperature environment or an extreme acid-base condition, the application range of the aflatoxin B1 degrading enzyme is remarkably limited and the like, the invention provides an aflatoxin B1 degrading enzyme mutant which can efficiently and rapidly degrade aflatoxin B1, and the aflatoxin B1 degrading enzyme mutant has the advantages that the aflatoxin B1 degrading enzyme mutant has a good application prospect. And the strain also has the characteristics of acid and alkali resistance and high temperature resistance, shows excellent performance in the aspect of aflatoxin B1 pollution treatment, and has a good industrial application prospect. Besides, food-grade safe strains such as saccharomyces cerevisiae, bacillus licheniformis or bacillus subtilis are used as host strains for preparing the aflatoxin B1 degrading enzyme mutant, the degrading effect can be guaranteed, meanwhile, the higher requirement for the safety of enzyme preparations in the feed field can be met, and the application of the aflatoxin B1 degrading enzyme mutant has more advantages.

The invention provides an engineering bacterium capable of efficiently degrading aflatoxin B1 as well as a construction method and application of the engineering bacterium. The engineering bacterium PD630:: lac103sp is obtained by taking rhodococcus turbid PD630 as an original strain and expressing a modified fmb-rL103 protein coding gene, and the nucleotide sequence of the modified fmb-rL103 protein coding gene is as shown in SEQ ID NO: 1. Compared with wild rhodococcus turbid PD630, the engineering bacterium PD630:: lac103sp provided by the invention has the advantages that the degradation efficiency of the engineering bacterium PD630:: lac103sp on aflatoxin B1 is obviously improved, the problems of insufficient degradation efficiency and poor application stability of an existing strain on aflatoxin B1 are solved, and a novel functional strain and a technical support are provided for pollution abatement of aflatoxin B1.

The invention discloses a cleaning agent composition and application. The cleaning agent composition is composed of an acidic cleaning agent with the pH value of 3.0-5.0 and an alkaline cleaning agent with the pH value of 7.0-9.0, wherein the volume ratio of the acidic cleaning agent to the alkaline cleaning agent is (0.01-3): (0.01-2). According to the cleaning agent composition provided by the invention, through scientific compounding of the food-grade oxidant and the food-grade surfactant, the natural color and complete form of peanuts can be kept after cleaning, surface stains and organophosphorus pesticide residues can be effectively removed, and the food safety and commodity value of the peanuts are greatly improved. When the cleaning agent composition is applied to peanut cleaning, the process is mild, no residue exists, the aflatoxin pollution risk possibly caused by hydrogen peroxide can be avoided, and an optimal green cleaning solution is provided for the peanut processing industry.

The invention relates to application of pseudomonas PSC001 in degradation of aflatoxin B1 and promotion of rumen fermentation, and aims to solve the problems of pollution of aflatoxin B1 in feed and poor ruminant rumen fermentation function at the same time. The invention discloses the new application of the pseudomonas PSC001 with the preservation number of CCTCC M 2023814 for the first time, namely degradation of aflatoxin B1 and promotion of rumen fermentation. The key technical means is as follows: the strain or the microbial inoculum thereof is applied to an AFB1 pollution system or daily ration of ruminants, and can realize efficient detoxification and growth promotion under specific conditions (such as 30-42 DEG C and pH 5.0-8.0) for 24-60 hours. The highest degradation rate of the strain to AFB1 can reach 65.20%, the yield of volatile fatty acid in rumen can be remarkably increased, a microflora is optimized, and the strain has the dual effects of efficient detoxification and rumen health improvement, and is high in safety and wide in application prospect.

Provided are: a novel strain of an aflatoxin production-inhibiting bacterium, which is a novel microorganism having a high activity for inhibiting the production of aflatoxin; an aflatoxin production inhibitor produced by an aflatoxin production-inhibiting bacterium comprising the novel strain; and an effective method for producing an inhibitor comprising an agent for improving the effect of the aflatoxin production-inhibiting bacterium. And a method for controlling aflatoxin contamination using the aflatoxin production inhibiting bacteria or the aflatoxin production inhibitor. The present invention relates to an aflatoxin production inhibitor comprising a culture of the genus Klebsiella sp. Or Raoultella sp., or a compound represented by general formula (1), and more specifically, to an aflatoxin production inhibitor comprising a culture of the genus Klebsiella sp. Or Raoultella sp., or a compound represented by general formula (1). In the formula (1), R is a straight-chain or branched alkyl group with 1-4 carbon atoms.

The invention discloses an application of a plant growth regulator 2-nitrophenol sodium salt in inhibition of aspergillus flavus growth and toxin synthesis, which can effectively inhibit spore germination and mycelial growth of aspergillus flavus, and has a minimum inhibitory concentration of 100 [mu] g / mL and a minimum bactericidal concentration of 200 [mu] g / mL. The invention further shows that the compound can destroy the integrity of the cell membrane of aspergillus flavus and the ultrastructure of the cell of aspergillus flavus, induce the accumulation of a large amount of active oxygen in the cell, and trigger the damage of mitochondrial function and energy metabolism disorder, thereby inhibiting the growth and toxin production of fungi. When the compound is added into feed, the growth of aspergillus flavus can be effectively inhibited, and the pollution of aspergillus flavus and aflatoxin can be remarkably reduced. Under the effective inhibitory concentration, the 2-nitrophenol sodium salt does not show obvious toxicity to HeLa cells, and has application safety. The invention provides a novel application and a technical scheme for developing a novel safe and efficient green prevention and control technology for preventing and controlling aspergillus flavus and toxin pollution thereof.

The invention discloses application of edible mushrooms and associated trichoderma thereof in synergistic inhibition of aspergillus flavus, and belongs to the technical field of microorganisms. According to the method, a trichoderma library with antagonistic ability is separated from the waste mushroom substrate through a three-stage screening system, two strains of trichoderma associated with pleurotus geesteranus have an outstanding aspergillus flavus antagonistic effect, and growth and toxin production of aspergillus flavus can be more efficiently inhibited under the cooperation of pleurotus geesteranus. Furthermore, a synergistic system of pleurotus geesteranus and associated trichoderma can effectively prevent and control aflatoxin B1 pollution loss caused by aspergillus flavus infection in peanuts, and has the effect of promoting peanut growth. According to the method, the waste edible mushroom substrate can be recycled, so that resource utilization of agricultural wastes is realized, and the blank in the field of biological detoxification can be filled up, so that the input-output ratio of agriculture is increased. Therefore, the method has a good application prospect.

The invention provides a peanut kernel aspergillus flavus pollution detection and nutritional ingredient prediction method based on hyperspectral imaging and deep learning. Visible-near infrared and short wave infrared hyperspectral images are collected, space-spectrum features of peanut kernels are extracted, a dual-channel attention mechanism module is introduced to fuse the features, a space-spectrum collaborative neural network model is constructed, and quantitative prediction of the content of moisture, protein and grease in the peanut kernels is achieved. And rapid, nondestructive and high-precision classification and identification are carried out on samples in different pollution stages. The method has the advantages of high detection speed, high accuracy and strong model robustness, is suitable for fungal pollution screening and nutritional quality monitoring in the links of processing, storage, circulation and the like of grain and oil and agricultural products, and has good application prospects and popularization values.

This invention belongs to the field of solid-phase microextraction fiber technology, specifically relating to covalent organic framework materials synthesized based on ionic liquid catalysis and solid-phase microextraction fibers, and their application in aflatoxin contamination early warning. This invention uses ionic liquids as solvents and catalysts in the synthesis reaction of covalent organic framework materials, rapidly synthesizing ionic liquid-containing COFs (IL-COFs) in an open system at room temperature and atmospheric pressure. The solid-phase microextraction fiber is obtained by coating stainless steel wire with IL-COFs and then curing it. Using this solid-phase microextraction fiber for the extraction of aflatoxin and its precursors achieves highly selective and efficient adsorption. Combining IL-COF-based solid-phase microextraction with ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS / MS) enables simultaneous, sensitive, and accurate determination of aflatoxin and its precursors in samples. Furthermore, this detection method can be used to obtain the dynamic relationship between aflatoxin and its precursors, thereby providing early warning of aflatoxin contamination.

The invention belongs to the technical field of wild jujube comprehensive utilization, and particularly relates to a wild jujube resource comprehensive utilization technology production line which comprises a cleaning device, a wild jujube pulp removing device, a kernel washing device and a fermentation treatment device. Wild jujubes are fed into the cleaning device through a first-stage lifting device, and the output end of the cleaning device is connected with the wild jujube pulp removing device through a second-stage lifting device; the wild jujube pulp removing device separates wild jujubes into wild jujube pulp and jujube pits, economic benefits are improved, pulp water generated by cleaning the jujube pits is filtered and recycled, a water decoding ecological base is arranged in an outer cavity of the fermentation tower, jujube pulp can be fermented while flowing when flowing through the outer cavity, continuous operation of a production line is achieved, and the production efficiency is improved. And the problem of pollution caused by aflatoxin is effectively avoided while the wastes are recycled.