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Bacillus subtilis and application of same in resisting aspergillus

A technology of Bacillus subtilis and Aspergillus flavus, applied in the direction of application, bacteria, and microbial-based methods, can solve the problems of not developing molding and economical biological control agents, etc.

Inactive Publication Date: 2013-03-13
中华人民共和国潍坊出入境检验检疫局
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far, no shaped and economical biological control agent products have been developed in China

Method used

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  • Bacillus subtilis and application of same in resisting aspergillus
  • Bacillus subtilis and application of same in resisting aspergillus
  • Bacillus subtilis and application of same in resisting aspergillus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0026] Screening of Antagonistic Bacteria against Aspergillus flavus

[0027] 1 Experimental materials

[0028] 1.1 Experimental strains

[0029] Sample source

[0030] Soil samples were collected from large peanut planting areas in Zhucheng, Weifang, and Laixi, Qingdao, for selective isolation in order to obtain target bacteria.

[0031] 1.2 Experimental Instruments

[0032] DHG Series Electric Heating Constant Temperature Blast Drying Oven (Shanghai Xinmiao Medical Instrument Manufacturing Co., Ltd.)

[0033] Precision Electronic Balance (Shanghai Minqiao Precision Scientific Instrument Co., Ltd.)

[0034] Electronic Universal Furnace (Beijing Yongguangming Medical Instrument Factory)

[0035] Stainless Steel Portable Sterilizer (Shanghai Shen'an Medical Instrument Factory)

[0036] Single-person double-sided ultra-clean workbench (Suzhou Antai Technology Co., Ltd.)

[0037] HZQ-C Air Bath Oscillator (Harbin Donglian Electronic Technology Development Co., Ltd.)

[00...

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Abstract

The invention discloses bacillus subtilis and an application of the same in resisting aspergillus. The bacillus subtilis has antagonistic action in aspergillus flavus. A method for screening the bacillus subtilis which has antagonistic action in the aspergillus flavus from soil comprises the following steps: a, bacteria isolating; b, aspergillus culturing and spore liquid preparing; c, aspergillus-resistant strain screening; and d, aspergillus-resistant strain rescreening. Through aflatoxin panel screening tests and toxin production aspergillus flavus antagonistic tests, aspergillus flavus antagonistic strains in the peanut cultivation fields and storage warehouses are screened. A method for reasonably and effectively screening the aspergillus flavus antagonistic strains is established, so that the appropriate required aflatoxin inhibition strains are obtained. The bacillus subtilis is applied to the easy aflatoxin contamination stages which are before and after harvesting crops of peanuts and the like and during storage periods and the like, the aflatoxin prevention and control levels of the crops of export peanuts and the like can be improved, export risks are lowered, and the export trade of the crops and products thereof are promoted.

Description

technical field [0001] The invention relates to the technical field of microorganisms and detection and application of microorganisms, in particular to a bacillus subtilis and its application in anti-aspergillus. Background technique [0002] As a natural toxin, aflatoxin is produced when peanuts are grown in the field. Aflatoxin toxins cannot be removed from the food chain even through good management practices. Biological control method is a newly emerging method, which mainly uses the antagonism between microorganisms to find microorganisms that have an inhibitory effect on Aspergillus flavus; or genetically modifies toxin-producing Aspergillus flavus through transgenic technology, so that non-toxic Aspergillus flavus becomes The dominant bacterial species that may contaminate food to achieve the control of aflatoxins. It is economical, effective, and will not cause secondary pollution to the environment. It is expected to control the aflatoxin pollution of peanuts from...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/68C12Q1/04A23L1/015C12R1/125A23L5/20
Inventor 宫小明张艺兵孙军李建军马荣桧金超田国宁丁葵英
Owner 中华人民共和国潍坊出入境检验检疫局
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