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75 results about "Selective isolation" patented technology

Rapid quantitative analysis of proteins or protein function in complex mixtures

InactiveUS7544518B2Facilitates quantitative determinationFacilitates quantitative determination of the absolute amountsComponent separationMaterial analysis by electric/magnetic meansIsotopic labelingProtein expression profile
Analytical reagents and mass spectrometry-based methods using these reagents for the rapid, and quantitative analysis of proteins or protein function in mixtures of proteins. The methods employ affinity labeled protein reactive reagents having three portions: an affinity label (A) covalently linked to a protein reactive group (PRG) through a linker group (L). The linker may be differentially isotopically labeled, e.g., by substitution of one or more atoms in the linker with a stable isotope thereof. These reagents allow for the selective isolation of peptide fragments or the products of reaction with a given protein (e.g., products of enzymatic reaction) from complex mixtures. The isolated peptide fragments or reaction products are characteristic of the presence of a protein or the presence of a protein function in those mixtures. Isolated peptides or reaction products are characterized by mass spectrometric (MS) techniques. The reagents also provide for differential isotopic labeling of the isolated peptides or reaction products which facilitates quantitative determination by mass spectrometry of the relative amounts of proteins in different samples. The methods of this invention can be used for qualitative and quantitative analysis of global protein expression profiles in cells and tissues, to screen for and identify proteins whose expression level in cells, tissue or biological fluids is affected by a stimulus or by a change in condition or state of the cell, tissue or organism from which the sample originated.
Owner:UNIV OF WASHINGTON

Single-phase grounding protection method and system

InactiveCN102420420ASatisfies the judgment of single-phase ground faultEmergency protective circuit arrangementsProtection systemEmbedded system
The invention provides a single-phase grounding protection method which is used for judging and isolating a single-phase grounding fault of a small-current grounding system. The single-phase grounding protection method comprises the following steps of: 102, synchronously acquiring the power frequency voltage of a bus of the small-current grounding system and current traveling waves of all outgoing lines corresponding to the bus in real time; 104, when the small-current grounding system is disturbed, determining a disturbance outgoing line according to the current traveling waves of all the outgoing lines; 106, determining whether the small-current grounding system has the single-phase grounding fault according to the power frequency voltage; and 108, determining that the disturbance outgoing line is a faulted outgoing line if the small-current grounding system has the single-phase grounding fault, sending a tripping instruction and insolating the faulted outgoing line. Correspondingly, the invention also provides a single-phase grounding protection system. By the single-phase grounding protection method and the single-phase grounding protection system, a requirement for judging the single-phase grounding fault of the small-current grounding system and a requirement for quick, reliable, sensitive and selective isolation can be met.
Owner:TSINGHUA UNIV

Control system for seabed processing system

A control system of modular seabed processing unit (100) using one of a process module as a test separator comprising a system-module (110) to which external lines are connected, the system-module (110) includes a first portion (31) of the system-module isolating and connecting means (30), for acting on received fluid. At least one inlet flow line (21) and at least one outlet flow line (81) being connected to the first portion (31) of the system-module isolating and connecting means (30) for selective isolation of system-module from, or connection of the system-module to the external flow lines by means of a second complementary portion (32) of a manifold (130). The connecting means (30) with which the first portion (31) of the system- module isolating and connecting means (30) is adapted to engage.; The system-module includes control means (60) for controlling operation of the system-module characterized in that the system-module (110) includes at least one actuating means (121) connected to a control means (60) wherein the actuating means (121) of the system-module (110) associated with diverter valve (131) situated at a manifold (130). The diverter valve (131) is connectable and disconnectable to fluid carrying conduits from production well (160, 170) and is enabled to control the direction of fluid flow into the system-module. The control means (60) is in communication with a master control station (150).; The temperature and/ or pressure measuring means (61, 62, 63, 64) and valves (36, 37) connected to control means (60) by control line (65) which is adapted to transmit process values to a host platform (140) and to receive the control signals from the host platform (140) via umbilical (80) through a power connector (70). Sensing means to measure pressure, temperature and flow parameter; and the um means 54 to boost the fluid to host 81 via outlet flow line (23).
Owner:ALPHA PERISAI SDN BHD +1

Kit for synchronously separating and identifying salmonella and shigella as well as preparation and application

The invention relates to a method for synchronously separating and identifying salmonella and shigella in enteric pathogenic bacteria, which mainly solves the technical problems of high detection-missing rate, complicated identification step, high cost and unintuitive screening result in the existing method for separating the salmonella and the shigella. The kit comprises: 1) a TSB (Trypticase Soy Broth) serving as an universal-type non-selective proliferous liquid; 2) a 10-folds concentrated TSB serving as a 10-folds concentrated universal-type non-selective proliferous liquid; 3) a selenite brilliant green-sulfanilamide proliferous liquid serving as a salmonella proliferous liquid; 4) a shigella proliferous liquid; 5) a xylose lysine deoxycholate agar plate; 6) a salmonella chromogenic agar plate; 7) a salmonella-shigella comprehensive biochemical identification tube comprising a first test tube and a second test tube; 8) a hydrogen sulphide filter paper strip; and 9) an indole filter paper strip. In the invention, typical colonial morphology corresponding to the growth of a selective isolation medium, simple biochemistry and reaction combination test of a specific enzyme and a substrate are adopted to identify two kinds of enteric pathogenic bacteria.
Owner:SHANGHAI MUNICIPAL CENT FOR DISEASE CONTROL & PREVENTION
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