74 results about "Cycloheximide" patented technology

The invention discloses a gluconoacetobacter sp. strain and a screening and purifying method. The screening and purifying method is characterized in that: defective fruits are used as raw materials; cycloheximide is used to suppress the growth of molds and other fungi, and plate enrichment cultivation, gradient dilution plate coating, primary screening, second screening and other methods are adopted for screening and purification to obtain the strain generating bacteria cellulose. According to the description in the ninth version of Bergey's Manual of Determinative Bacteriology, the strain is determined as a novel strain by the analysis of physiological and biochemical properties and 16 Sr DNA sequence. The invention strain obtained by the method has a wide selection range of carbon sources and nitrogen sources, a maximum yield of bacteria cellulose at a pH value of 6.5 and normal temperature, and a higher yield than that of common cellulose-generating bacteria, and has better implementation prospect in the industrial production of bacteria cellulose.

The invention discloses a legionnella selective separation medium. Dyes such as bromothymol blue and bromocresol purple are added to the basic formulation of the basic medium of legionnella BCYE alphaand legionnella selective antibiotics of glycin, vancocin, polymyxin B and actinone are also added. The added dyes produce pigment in the growth process of legionnella; the shape and the color of a colony are beneficial to identifying and distinguishing a target bacterium of the legionnella; the concentrations of the selective antibiotics are added and regulated, the inhibiting capability to non-legionnella and microbial class is enhanced and the inhibiting performance for the legionnella is reduced, thereby enhancing the positive rate for the separation culture of the legionnella; the detection cost is reduced and the legionnella selective separation medium is economic and practical and is convenient for popularization and application for clinical and health and epidemic prevention departments.

The invention discloses a bacillus cereus as well as, a screening method, a preparation and application thereof. The bacillus cereus has efficient oxytetracycline degrading capability, and is screened according to the following method: (1) selecting pig manure bacterial suspension contaminated by oxytetracycline, and culturing single bacterial colonies in a culture medium containing 400-500mug/ml of cycloheximide; (2) continually improving the concentration of the oxytetracycline in the culture medium, to retain adaptive single bacterial colonies; and (3) culturing the single bacterial colonies in an inorganic salt culture medium, continually improving the concentration of the oxytetracycline, and preserving bacteria of which the oxytetracycline degrading rate is not less than 50% and the OD600 is not less than 0.2. The preparation comprises dried bacillus cereus powder and organic matrix in a mass ratio of 1:4 to 1:5. The bacillus cereus is applied to pig manure composting, has strong adaptability, high oxytetracycline degrading rate, low environmental influence and low cost, and is suitable for large-scale application.

The invention provides a seed coating agent using cycloheximide as the effective composition, which is prepared through isolating and extracting active ingredient from the microbial cultivation substance of SPRI-201 (Streptomyces genius) CGMCC No.1317, then mixing with carrying agent and auxiliary agent.

The invention relates to ionic liquid and a preparation method thereof, in particular to ionic liquid taking imidic nitrogen heterocyclic rings as positive ions and a preparation method of the ionic liquid. The ionic liquid has the following chemical structure formula, wherein n is equal to 3 or 4; and Y- is selected from the following negative ions: chlorine, bromine, iodine, sulfate radical, bisulfate radical, nitrate radical, dihydrogen phosphate radical, fluoroboric acid radical, hexafluorophosphoric acid radical, acetate moiety, methanesulfonic acid radical, p-toluenesulfonic acid radical, trifloromethanesulfonic acid radical or trichloroacetic acid radical. Cycloheximide is linked into sulfonic groups through a quaternary amination reaction; the sulfonic groups are dissolved into water; acid is added into the mixture; heating the mixture to perform a reaction; then a reaction product is distilled and purified to obtain the ionic liquid. The ionic liquid has simple preparation method, low preparation cost, strong acidity and excellent catalytic activity on esterification and the like for acid catalyzed reaction.

The invention relates to phylum porifera symbiosis streptomycete SP6206, belonging to the field of phylum porifera symbiosis streptomycete fermentation. The preservation number of the phylum poriferasymbiosis streptomycete SP6206 is CCTCCM2017451. The invention also discloses a method for producing staurosporine by fermenting the phylum porifera symbiosis streptomycete SP6206, which comprises thefollowing steps: (1) culturing a seed solution of the SP6206; (2) inoculating the cultured seed solution of the SP6206 to a fermenting culture substrate, and carrying out fermented culture, thereby obtaining a fermentation solution; and (3) separating staurosporine from the fermentation solution. According to the phylum porifera symbiosis streptomycete SP6206, the highest yield of the staurosporine reaches 320mg/L and is greatly increased compared with that of the common wild streptomycete staurosporine in the prior art. The staurosporine can be used for preventing and treating the colletotrichum gloeosporioides of Chinese yams, the minimal inhibitory concentration of the staurosporine for preventing and treating the colletotrichum gloeosporioides of the Chinese yams is 8 microg/ml, and the minimal inhibitory concentration of the staurosporine is 1/4 that of the positive drug cycloheximide.

The present invention discloses a production method of cyclohexylimine and its process. It is characterized by that it adopts solid caprolactam and hydrogen gas and makes them implement reduction reaction in a certain container so as to produce the invented product cyclohexylimine.

The invention relates to an RNC binding agent and method for extracting RNC-RNA (ribosome nascent-chain complex and ribonucleic acid) from blood, wherein the RNC binding agent comprises the following components by content: 120-180 Mug/ml of cycloheximide, 25-35 mM of dithiothreitol, 0.45-0.65 M of ammonium chloride, 25-35 mM of sodium citrate, 25-35 mM of ethylene diamine tetraacetic acid, 10-20 mM of ethylene glycol bis(2-aminoethyl ether)tetraacetic acid, 15-25 MuM of 1,2,3,4,6-O-pentagalloylglucose, 0.005-0.015% of glycerol, and the balance of water. The RNC binding agent (RNC blood binding solution) of the invention is a multifunctional reagent integrating translation extension inhibitor, RNA protectant and red blood cell lysate and may serve as an RNA protectant for use in the collection of conventional samples.

The invention relates to a chemical agent for thinning and removing plant flying catkins and flying fur. The chemical agent comprises the following active compound: a plant growth regulator, a plant fruit thinning agent and a herbicide in a weight ratio of (1 to 10):(1 to 10):(5 to 100). The plant growth regulator comprises one or more of forchlorfenuron, 6-benzyladenine, paclobutrazol, uniconazole, 2,4-dichlorphenoxyacetic acid, triiodobenzoic acid, 2-chloroethyl trimetyl ammonium chloride and cycloheximide. The plant fruit thinning agent comprises one or more of ethychlozate and chlorpropham. The herbicide comprises one or more of a sulfonylurea herbicide, an imidazolinone herbicide and a triazolopyrimidine herbicide. The chemical gent is capable of inhibiting growth and development of flowers and buds of plants of aspens, willows and plane trees and the like, thereby achieving the preventing and treating effect on the flying catkins and the flying fur. The invention further relatesto application of the chemical agent for thinning and removing the plant flying catkins and flying fur. The chemical agent is applied to the plant in an injection mode or a spray mode, so the purposesof inhibiting expansion of the flowers and buds of the plants and preventing and treating the flying catkins and the flying fur are realized.

The invention relates to a formula and preparation method of a culture medium used for a strain of actinomycetes Niannianle: ACCC40027 streptomyces microflavus, from cultivation and storage to fermentation production; moreover, the culture medium can ensure high yield and hardly degraded. And the following special materials are added: mycose, cycloheximide and potassium dichromate.

A process for separating antibacterial marinomicromonal includes such steps as providing specimen, taking upper bacterial suspension, preparing culture medium, K7Cr2O7 solution and actidione solution, respective filter for removing bacteria, pouring on plate, separating micromonad, inoculating to sant, culturing, and choosing vinaceous strains. Its advantages are high antibacterial activity and high screening efficiency.

The invention relates to the bacterium separation technology, in particular to a direct quantitative counting method of campylobacter in food. The method comprises the following steps: culturing samples on a solid CCDA culture medium containing growth promoters and six kinds of antibiotics such as polymyxin B, trimethoprim, rifampicin, actidione, cefoperazone and fungizone B; then, directly carrying out campylobacter counting and separation. Compared with a bacterium culture, separation and certification method of a traditional method, the invention has the advantages of simplicity, convenience, specificity and accuracy.

The invention discloses a composite enrichment culture medium capable of simultaneously enriching three food-borne pathogenic bacteria, namely Listeria monocytogenes, Escherichia coli O157:H7 and salmonella, and a preparation method. The formula of the culture medium comprises the following components of, in parts by weight, 15.0-19.0 parts of tryptone, 2.7-3.3 parts of peptone, 5.5-6.5 parts of yeast extract, 4.5-5.5 parts of sodium chloride, 2.25-2.75 parts of dipotassium phosphate, 8.6-10.6 parts of disodium hydrogen phosphate, 1.22-1.48 parts of potassium dihydrogen phosphate, 24.0-30.0 parts of peptone, 14.0-18.0 parts of beef powder, 10.0-14.0 parts of soy peptone, 2.0-5.0 parts of glucose, 0.10-0.30 part of actinomycete ketone, 2.5-3.5 parts of mannitol, 4.5-6.5 parts of sodium pyruvate, 0.00125-0.00300 part of acridine yellow, 0.0007813-0.0032500 part of fosfomycin sodium and 1000 parts of distilled water, and the pH value of theculture medium is 6.8-7.4. The single enrichmenteffect of the composite enrichment culture medium is superior to that of respective selective enrichment liquid, growth of non-target bacteria can be well inhibited, under the condition that non-target bacteria exist, the culture medium can further achieve constant-speed and rapid proliferation of the three target bacteria.

The invention discloses a nitrogen element synergistic agent in straw-application soil and a preparation method thereof. The synergistic agent consists of the following raw materials in certain mixture ratio: cycloheximide or natamycin, tetracycline or oxytetracycline, streptomycin, silica, a wetting agent NNO and nekal BX. The preparation method comprises the following steps: A.. the cycloheximide or the natamycin, the tetracycline or the oxytetracycline, the streptomycin and the silica are premixed according to the proportion; B. the wetting agent NNO and the nekal BX is added to the obtained mixture, are subjected to secondary mixing; and C. the obtained mixture is dried, crushed, sifted and packaged under certain condition. The synergistic agent has the advantages of simple method, small energy consumption, no pollution to environment, long quality guarantee period, convenient use, safe operation, low cost, no toxicity and no side effect on human, animal and plant and safety to biological environment; and the effective nitrogen content of the straw-application soil is increased by 50 to 120 percent, thereby alleviating crop pest in straw application. The method is suitable for application to improve effective nitrogen in soils with difficult types when various crop straws are returned to the field or the soil.

The invention discloses an apparatus and method for detecting concentration of a cycloheximide solution. The apparatus comprises a computer, an acquisition unit and an acquisition workbench. The acquisition unit comprises a cell plate electrode, a surface acoustic wave resonator and a conversion unit. The acquisition workbench comprises a pedestal; a standing groove is arranged on the pedestal and is internally provided with a location base; the location base is provided with a location groove; a dropping chamber is arranged above the standing groove; the bottom of the dropping chamber is provided with a micro pump communicated with the dropping chamber; the bottom of the micro pump is provided with a dropping head communicated with the micro pump; the top of the dropping chamber is provided with a liquid inlet; the dropping chamber is also internally provided with a cleaning mechanism and a draining mechanism; a disc is arranged above the dropping chamber; a plurality of liquid storage chambers are arranged on the disc around the center of circle of the disc; the bottom of each liquid storage chamber is provided with a liquid outlet at a position corresponding to the liquid inlet; the liquid outlets are provided with liquid outflowing solenoid valve; and the top of the dropping chamber is provided with a driving motor drives the disc to rotate. The apparatus has a simple structure and low cost and can rapidly and accurately detect the concentration of the cycloheximide solution.

The invention provides a candida utilis expression vector for producing laccase, a construction method of the candida utilis expression vector, recombinant engineering bacteria and an application of the recombinant engineering bacteria. The candida utilis expression vector for producing the laccase comprises a 18S rDNA gene segment, a yeast glyceraldehyde triphosphate dehydrogenase gene promoter sequence (GAP-p), a laccase gene, a yeast glyceraldehyde triphosphate dehydrogenase gene terminator sequence (GAP-t) and a cycloheximide (CYH) resistance gene. According to the invention, all genetic elements of the whole expression system are from probiotic candida utilis and non-toxic and harmless fungi, antibiotic resistance genes are not carried, drifting, diffusion and integration of the resistance genes do not exist, other toxic protein genes are not carried, biosafety hazards to the environment or people and animals are avoided, the food grade is achieved, and the recombinant engineeringbacteria can be directly added into foods, medicines and feeds for application.

The invention discloses a method for treating wastewater containing cycloheximide and hexamethylene diamine and belongs to the technical field of wastewater treatment. The method comprises the following steps that the wastewater containing cycloheximide and hexamethylene diamine is mixed and contacted with a fibrous adsorbent in a container or is contacted with the fibrous adsorbent in an absorption column in a flowing mode, so that cycloheximide and hexamethylene diamine in the wastewater are adsorbed by carboxyl on fibers; and after the fibers adsorb cycloheximide and hexamethylene diamine to be saturated, the obtained product is subjected to elution regeneration by diluted acid and the product can be used continuously repeatedly. The method has high adsorbing speed, high sensitivity and high adsorption capacity for amine. High-concentration amine hydrochloride in eluent can be recycled as required.

The invention discloses an apparatus and method for detecting the concentration of cycloheximide based on a biological tongue. The apparatus is electrically connected with a computer and comprises a bedstand, a detection box, a surface acoustic wave resonator, an electronic counter, an oscillator, a wireless emitter and a cell electrode chip, wherein the detection box, the surface acoustic wave resonator, the electronic counter, the oscillator, the wireless emitter and the cell electrode chip are arranged on the bedstand, the oscillator and the surface acoustic wave resonator compose an oscillation circuit, the electronic counter is electrically connected with the wireless emitter and the oscillation circuit, the surface acoustic wave resonator is electrically connected with the cell electrode chip, and the wireless emitter is in communication connection with the computer. The method has the advantages of simple operation, good detection sensitivity, high detection precision and a fast detection speed; and the apparatus has the characteristics of good stability and repeatability.

The invention relates to a pesticide blending technology, wherein nimbin and cycloheximide are employed as the main raw material to blend into oilsuspending agent, which have the advantages of improved preventing and curing effect, safety to human, livestock and ecologic environment, Thus can serve as a blended germicidal agent for widespread application.

The invention discloses a culture medium for separating brucella in marmot tissues and a preparation method thereof. The culture medium is specifically composed of components: agar, tryptose, sodium chloride, anhydrous sodium sulfite, a yeast extract, bovine liver extract powder, D-glucose, erythritol, sodium citrate, special-grade horse serum, bacitracin, actinomycete ketone and distilled water or double distilled water. The culture medium is a solid culture medium capable of culturing brucella and is balanced in nutrition; the added components not only meet a carbon source, a nitrogen source and the like required by the metabolism of the brucella, but also inhibit the growth of other bacteria in a polluted sample, so that the detection rate of the brucella in the polluted sample is improved; all the components supplement each other to achieve a synergistic effect; and the culture medium provided by the invention can meet the growth and propagation requirements of separating brucella from marmot himalayan tissues, and has the advantages of stable quality, simplicity and convenience in preparation and high number of cultured bacteria.

The invention discloses a thinning chemical for plant pollen, belonging to the technical field of plant growth regulation. The main points of a technical scheme of the invention are as follows: the thinning agent comprises an original chemical and an auxiliary agent, wherein the original chemical is one or more selected from the group consisting of forchior fennron, ethychlozate, 6-benzyladenine,a gibberellin compound, paclobutrazol, uniconazole, naphthaleneacetic acid, indolebutyric acid, 2,4-dichlorophenoxyacetic acid, triiodobenzoic acid, 2-chloroethyl trimetyl ammonium chloride, cycloheximide, chlorpropham, a sulfonylurea herbicide, an imidazolinone herbicide and a triazolopyrimidine herbicide. The invention also discloses an application method for the above-mentioned chemical. According to the invention, in a plant staminate strobilus swelling period, the chemical is diluted to 0.04 to 8 g of the original chemical per L of the chemical for foliar application, or the chemical is injected into the trunk of a plant according to a dosage of 0.1 to 5 g of the original chemical per cm of diameter at breast height, so tree species like platycladus orientalis, sabina chinensis, ginkgo biloba and pinus tabuliformis can be effectively inhibited from generating pollen, and the effect of thinning is excellent.

The invention provides a detection medium for acetobacter and Gluconobacter cerinus. Detection functional ingredients in the medium comprise yeast extract, glucose, inorganic salt, ethanol and cycloheximide. During detection of acetobacter and Gluconobacter cerinus, the ingredients of the medium show characteristic red, thus enhancing the effect of isolation and identification. By adding sodium chloride, dipotassium hydrogen phosphate and magnesium sulfate as ingredients of the differential medium, color developing time of the acetobacter and Gluconobacter cerinus is shortened, and the isolation and identification efficiency is enhanced. By adding calcium carbonate as an ingredient of the differential medium, the differential medium can be used in isolation and identification of acetobacter and Gluconobacter cerinus for high yield of acetic acid, and is applicable to the industry of edible vinegar and acetic acid.