Separation method for detecting antibiotic class group of oceanic Micromonospora

Abstract

A process for separating antibacterial marinomicromonal includes such steps as providing specimen, taking upper bacterial suspension, preparing culture medium, K7Cr2O7 solution and actidione solution, respective filter for removing bacteria, pouring on plate, separating micromonad, inoculating to sant, culturing, and choosing vinaceous strains. Its advantages are high antibacterial activity and high screening efficiency.

Description

technical field

[0001] The invention relates to a method for separating and detecting Micromonospora, in particular to a method capable of selectively separating and detecting antimicrobial groups of Micromonospora marine. Background technique

[0002] Micromonospora (Micromonospora) is a rare genus of actinomycetes, and usually exists in soil, ocean and other habitats in small quantities. In recent years, because this genus can produce various antibacterial and antitumor active substances, it has attracted people's attention widely.

[0003] Yang Yurong (Yang Yurong, Journal of Yunnan University, 1997, 19(4): 403-408, hereinafter referred to as document 1) reported the isolation technology of rare actinomycetes including Micromonospora, but the separation of YIM medium used The numbers are usually lower. Li Wenjun (Li Wenjun, Foreign Pharmaceutical Antibiotics Volume, 2002, 23(1): 18-22, hereinafter referred to as document 2) recommended soil air-drying, phenol treatment ...