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Separation method for detecting antibiotic class group of oceanic Micromonospora

A micromonospora and marine technology, which is applied in the field of separation and detection of Micromonospora, can solve problems such as the detection technology of antibacterial strains that are not involved

Inactive Publication Date: 2005-06-15
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above documents do not involve the detection technology of antibacterial strains

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1) Prepare the sample: air-dry the sample for 15 days, pulverize it, pass through a 50-mesh sieve, dry heat treatment at 120°C for 1.2h, weigh 5g and dissolve it in 45ml of sterile water (homogenization treatment by homogenizer for animal, plant and rotting plant residues), Add phenol at a final concentration of 1%, bathe in water at 40° C. (intermittent shaking) for 20 minutes, and let stand at room temperature for 5 minutes. The upper bacterial suspension is used for separation.

[0024] 2) Configure the modified Gaoshi No. 1 medium: 2% soluble starch, KNO 3 0.5%, K 2 HPO 4 0.04%, MgSO 4 0.06%, agar 1.5%, Yu Chen sea water. Sterilize at 121°C for 25 minutes.

[0025] 3) Configure K 7 Cr 2 o 7 0.5% solution; Cycloheximide 0.5% solution. Sterilize by filtration with a 0.2 μm microporous membrane.

[0026] 4) Invert the plate: take the culture medium that has just been sterilized, and make the K in the culture medium 7 Cr 2 o 7 The final concentrations of...

Embodiment 2

[0031] 1) Prepare the sample: air-dry the sample for 18 days, pulverize it, pass through an 80-mesh sieve, dry heat treatment at 115°C for 1.5 hours, weigh 5g and dissolve it in 25ml of sterile water (homogenized by a homogenizer for animal and plant residues and rotting plant residues), Add phenol with a final concentration of 0.8%, bathe in water at 45° C. (intermittent shaking) for 25 minutes, and let stand at room temperature for 10 minutes. The upper bacterial suspension is used for separation.

[0032] 2) Configure the modified Gaoshi No. 1 medium: 3% soluble starch, KNO 3 1.5%, K 2 HPO 4 0.05%, MgSO 4 0.05%, agar 2%, Yu Chen sea water. Sterilize at 121°C for 20 minutes.

[0033] 3) Configure K 7 Cr 2 o 7 0.5% solution; Cycloheximide 0.5% solution. Sterilize by filtration with a 0.2 μm microporous membrane.

[0034] 4) Invert the plate: take the culture medium that has just been sterilized, and make the K in the culture medium 7 Cr 2 o 7 The final concent...

Embodiment 3

[0039] 1) Prepare the sample: air-dry the sample for 20 days, pulverize it, pass through a 40-mesh sieve, and dry heat at 118°C for 2 hours, weigh 5g and dissolve it in 70ml of sterile water (homogenized by a homogenizer for animal and plant residues and rotting plant residues), add The final concentration is 1.2% phenol, 35 ° C water bath (intermittent shaking) for 30 min, room temperature for 8 min, and the upper bacterial suspension is used for separation.

[0040] 2) Configure the modified Gaoshi No. 1 medium: 1% soluble starch, KNO 3 1%, K 2 HPO 4 0.05%, MgSO 4 0.04%, agar 1.5%, Yu Chen sea water. Sterilize at 121°C for 30 minutes.

[0041] 3) Configure K 7 Cr 2 o 7 0.5% solution; Cycloheximide 0.5% solution. Sterilize by filtration with a 0.2 μm microporous membrane.

[0042] 4) Invert the plate: take the culture medium that has just been sterilized, and make the K in the culture medium 7 Cr 2 o 7 The final concentrations of the solution and cycloheximide...

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PUM

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Abstract

A process for separating antibacterial marinomicromonal includes such steps as providing specimen, taking upper bacterial suspension, preparing culture medium, K7Cr2O7 solution and actidione solution, respective filter for removing bacteria, pouring on plate, separating micromonad, inoculating to sant, culturing, and choosing vinaceous strains. Its advantages are high antibacterial activity and high screening efficiency.

Description

technical field [0001] The invention relates to a method for separating and detecting Micromonospora, in particular to a method capable of selectively separating and detecting antimicrobial groups of Micromonospora marine. Background technique [0002] Micromonospora (Micromonospora) is a rare genus of actinomycetes, and usually exists in soil, ocean and other habitats in small quantities. In recent years, because this genus can produce various antibacterial and antitumor active substances, it has attracted people's attention widely. [0003] Yang Yurong (Yang Yurong, Journal of Yunnan University, 1997, 19(4): 403-408, hereinafter referred to as document 1) reported the isolation technology of rare actinomycetes including Micromonospora, but the separation of YIM medium used The numbers are usually lower. Li Wenjun (Li Wenjun, Foreign Pharmaceutical Antibiotics Volume, 2002, 23(1): 18-22, hereinafter referred to as document 2) recommended soil air-drying, phenol treatment ...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
Inventor 黄耀坚郑忠辉徐庆妍宋思杨苏文金
Owner XIAMEN UNIV
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