55 results about "Micromonospora" patented technology

The invention belongs to the technical field of microbe application and discloses a microbial agent for promoting quick decomposition of agricultural wastes, prepared from the following steps of respectively carrying out first stage amplification culture and second stage amplification culture on four microbes which are bacillus subtilis, micromonospora streptomyces, trichoderma koningii and chaetomium globosum, and respectively obtaining four liquid cultures of the microbes; then commonly inoculating the four liquid cultures in a carrier culture medium according to a ratio with the inoculation amount of 0.2 percent, controlling the moisture content at 55-60 percent, culturing for 7 days at a constant temperature of 28 DEG C; and finally, carrying out low-temperature drying treatment on a complex bacteria subjected to culturing at the temperature of 35-40 DEG C, and carrying out live bacteria detection. The microorganism composting microbial agent can promote quick decomposition of agricultural waste aerobic composts, has remarkable action of eliminating malodorous gas generated by organic wastes and also reduces the nutrient loss caused by volatilization in a composting process.

The invention discloses a wheat straw compound fertilizer for slowly releasing drought stress. The wheat straw compound fertilizer is prepared from the following components in parts by weight: 1000 parts of dry straws, 2000 parts of biogas slurry, 400-500 parts of urea, 60-90 parts of monopotassium phosphate, 80-110 parts of boric acid, 40-50 parts of chromium trichloride, 40-50 parts of manganese chloride, 100-120 parts of zinc sulfate, 2-5 parts of SA (salicylic acid), 2-5 parts of ethephon, 20-30 parts of polyaspartic acid, 15-20 parts of trichodermaspp, 9-15 parts of micromonospora and 20-30 parts of polyangium cellulosum. The wheat straw compound fertilizer is prepared through the following steps of: selecting biogas slurry; implementing microorganism treatment; implementing aerobic fermentation; implementing anaerobic fermentation; adding boric acid, chromium trichloride, manganese chloride, zinc sulfate, SA, ethephon and polyaspartic acid in a fermentation tank and evenly mixing to form a mixture; bagging and packaging the mixture.

The invention relates herba dracocephalum heterophylli volatile oil possessing the bacteriostatic and germicidal action and its application. The method adopts steam distillation to extract the herba dracocephalum heterophylli volatile oil and adopts gas-chromatography/mass spectra to analysis the volatile oil, identifying the 83 components and 89.83% total content. The volatile oil can kill and suppress the staphylococcus, streptococcus, gonococcus, enterobacter, Escherichia, salmonella, shigella, pseudomonas, bacillus, oidiomycin, cryptococcus, aspergillus, penicillium, mucor, epidermophytin, micromonospora, trichophyton and other bacteriums. The herba dracocephalum heterophylli volatile oil is used for foodstuffs, drugs, health products, cosmetic products and other industries.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a marine Micromonospora strain for fermentation production of Rakicidin B1, and an application thereof. An existing marine micromonospora FIM02523 for producing Rakicidins compounds undergoes mutation breeding to obtain a mutant strain marine Micromonospora FIM-R160609 for highly yielding RakicidinB1. The mutant strain can effectively increase the tilter of the Rakicidin B1 in a fermentation broth. In a fermentation experiment using a 20-1000 L fermenter, the tilter of the Rakicidin B1 producedby the marine Micromonospora FIM-R160609 reaches up to about 600 mg/L, and a ratio of the component B1 to the component B in the fermentation broth is equal to or more than 97:3 so the fermentation yield of the target product Rakicidin B1 is greatly increased, the yield of other Rakicidins byproducts is effectively reduced, subsequent extraction and purification of the Rakicidin B1 are facilitated, and industrialization demands can be met.

The invention belongs to the technical field of medicine, and relates to construction of biosynthesis gentamicin X2 gene engineering bacteria and application thereof. By the adoption of a microorganism molecular genetics technology, biosynthesis key genes of gentamicin in micromonospora purpurea are inactivated, gentamicin biosynthesis metabolic flux is cut off, and gentamicin X2 is directionally accumulated. By constructing recombinant plasmid pFU503 and leading pFU503 into micromonospora purpurea GK1101, the function of (i) genB3 (/i) is inactivated through an in-frame knockout technology, double exchange bacterial strains are screened, fermentation products are detected, new components are identified, and gentamicin X2 producing bacteria are fixed. The constructed engineering bacteria have stable hereditary features, can accumulate a large number of gentamicin X2, and fill the foreign and domestic research gap. The constructed engineering bacteria production technology is simple, fermentation control is easy, the synthetic efficiency is high, extraction and purification are convenient, the product quality is stable, clean production state is nearly achieved, the bacteria have an important industrial application value, and great economic benefit can be produced.

The invention discloses a cotton straw compound fertilizer for slowly releasing low-temperature stress. The cotton straw compound fertilizer is prepared from the following components in parts by weight: 1000 parts of dry straws, 2000 parts of biogas slurry, 200-300 parts of urea, 50-70 parts of monopotassium phosphate, 40-60 parts of boric acid, 40-50 parts of chromium trichloride, 50-80 parts of manganese chloride, 100-120 parts of zinc sulfate, 12-20 parts of polyaspartic acid, 12-20 parts of aspergillus flavus, 9-15 parts of micromonospora and 20-30 parts of polyangium cellulosum. The cotton straw compound fertilizer is prepared through the following steps of: selecting biogas slurry which can normally generate methane; implementing aerobic fermentation; implementing anaerobic fermentation; adding boric acid, chromium trichloride, manganese chloride, zinc sulfate and polyaspartic acid to form a mixture; evenly mixing the mixture; bagging and packaging the mixture.

The invention discloses a tobacco straw compound fertilizer for slowly releasing drought stress. The tobacco straw compound fertilizer is prepared from the following components in parts by weight: 1000 parts of dry straws, 2500 parts of biogas slurry, 200-300 parts of urea, 50-70 parts of monopotassium phosphate, 80-110 parts of boric acid, 60-80 parts of chromium trichloride, 50-80 parts of manganese chloride, 100-120 parts of zinc sulfate, 16-25 parts of polyaspartic acid, 15-20 parts of trichodermaspp, 9-15 parts of micromonospora and 20-30 parts of polyangium cellulosum. The tobacco straw compound fertilizer is prepared through the following steps of: selecting biogas slurry which can normally generate methane; implementing aerobic fermentation; implementing anaerobic fermentation; adding boric acid, chromium trichloride, manganese chloride, zinc sulfate and polyaspartic acid in a fermentation tank to form a mixture; evenly mixing the mixture; bagging and packaging the mixture.

The invention belongs to the technical field of microorganism fermenting, and particularly relates to a marine micromonospora strain for fermenting to produce Rakicidin B and application thereof. Theexisting marine micromonospora strain FIM02523 for producing Rakicidins compound is induced to breed, so as to obtain a mutation marine micromonospora strain FIM-R160708 for producing the Rakicidin Bat high output. The marine micromonospora strain has the advantages that the valence of the Rakicidin B in a fermenting solution can be effectively increased; in the fermenting experiment of a 20-1000L fermenting tank, the valence of the Rakicidin B fermented by the marine micromonospora strain FIM-R160708 reaches about 1000mg/L, the ratio of components B1 to B in the fermenting solution is smaller than or equal to 3:97, the fermenting output of the target product Rakicidin B is greatly increased, and the production rate of other Rakicidins byproducts is effectively reduced; the subsequent extracting and purifying of the Rakicidin B can be favorably performed, and the industrial requirements are met.

The invention belongs to the technical field of microorganism fermenting, and particularly relates to a marine micromonospora strain for fermenting to produce Rakicidin A and application thereof. Theexisting marine micromonospora strain FIM02523 for producing Rakicidins compound is induced to breed, so as to obtain a mutation marine micromonospora strain FIM-R150103 for producing the Rakicidin Aat high output. The marine micromonospora strain has the advantages that the valence of the Rakicidin A in a fermenting solution can be effectively increased; in the fermenting experiment of a 20-1000L fermenting tank, the valence of the Rakicidin A fermented by the marine micromonospora strain FIM-R150103 reaches about 300mg/L; the extracting and purifying of the Rakicidin A can be favorably performed, and the industrial requirements are met.

The invention discloses micromonospora haikouensis having algae dissolving capacity and an application of the micromonospora haikouensis to karenia mikimotoi, and belongs to the microbiology field fortreatment of harmful red tide. The algae dissolving fungi is named as Micromonospora haikouensis B2572 ( hereinafter referred to as Micromonospora haikouensis B2572 or bacterial strain B2572 ), separated from roots of sonneratia apetala in Maowei sea mangrove nature reserve in Qinzhou city of Guangxi province, and is preserved in China Center for Type Culture Collection at 22 May, 2019, with thepreservation No. of CCTCC NO:M 2019382. The new bacterial strain provided by the invention has good restraining and killing effect on Beibuwan bay sea area dominant algae namely the Karenia mikimotoi,under the condition of treatment for 24h, the algae dissolving rate can achieve 90% or above, and the micromonospora haikouensis has broad application prospects in harmful algal bloom events broken out in massive water bodies.

The invention discloses a deodorizing compound inoculum for feces of livestock and poultry and a preparation method of the deodorizing compound inoculum, and relates to the field of microbe applications. The deodorizing compound inoculum and the preparation method are provided aiming at the problems that the technical cost for chemical deodorization is high, the reaction is complicated, secondarypollution is generated, and the commercial biological deodorant is poor in adaptation and single in function. The deodorizing compound inoculum for feces of livestock and poultry comprises five species of bacteria including bacillus amyloliquefaciens, Alcaligenes faecalis subsp.faecalis, Arthrobacter humicola, Streptomyces levoris and Micromonospora yulongensis. The preparation method of the deodorizing compound inoculum comprises the following steps: fermentation of single bacterial strains, preparation of the compound inoculum, and adding of a fresh culture medium, an activating agent and aprotection agent. The technical scheme has the beneficial effects that the treatment speed and utilization ratio for feces are improved, and the deodorizing effect is rapid and lasting.

A process for separating antibacterial marinomicromonal includes such steps as providing specimen, taking upper bacterial suspension, preparing culture medium, K7Cr2O7 solution and actidione solution, respective filter for removing bacteria, pouring on plate, separating micromonad, inoculating to sant, culturing, and choosing vinaceous strains. Its advantages are high antibacterial activity and high screening efficiency.

The invention belongs to the industrial biotechnology field and provides an actinomycetes culture medium and application thereof. The actinomycetes culture medium consists of the following basic compositions: a yeast extract, peptone, Ca<2+>, Zn<2+>, Cu<2+>, Fe<2+> and a carbon source; the mass ratio of the above compositions is (2-10):(1-8):(0.01-0.2):(0.002-0.03):(0.0002-0.003):(2-14); distilledwater is dissolved and diluted, and the concentration of the yeast extract is controlled to be 2-12 g/L. The actinomycetes culture medium and the application thereof provided by the invention have the benefits that micromonospora and fiberomonas bacterial strains cultured by the actinomycetes culture medium can produce fibrous body analogues; one of enzymes has the activity of hydrolyzing a DAXPC-7 glycosidic bond of a taxol precursor; the enzyme production efficiency is relatively high, so that the synthetic pathway of taxol can be greatly shortened, the cost is reduced, the environmental pollution is reduced, and the like; therefore, the actinomycetes culture medium is an excellent enzyme preparation for preparation of a pharmaceutical intermediate for industrial microbial transformation.

The invention discloses a bio-fermentation agent for promoting water hyacinth decomposition. The bio-fermentation agent comprises a compound bacteria agent, nutritional ingredients, soluble carriers and a microelement chelate, wherein the compound bacteria agent comprises two or more of bacillus subtilis, trichoderma viride, phanerochaete chrysosprium, Nocardia and micromonospora. The invention also discloses a preparation method for the bio-fermentation agent. The preparation method comprises the following steps: preparing compound bacteria species, culturing the compound bacteria species to obtain the compound bacteria agent, and finally, mixing the obtained the compound bacteria agent, the nutritional ingredients, the soluble carriers and the microelement chelate in proportion to obtain the required bio-fermentation agent. The fermentation agent can be manufactured to powder and be dissolved in water completely, a use method is simple, and the propagation of pathogenicity microorganism and incubation of ova can be inhibited to prevent the pest and disease damages; after the fermentation agent is mixed with water hyacinth residual bodies, the decomposition of the water hyacinth residual bodies can be obviously promoted and nutrient content is improved. The method is simple and easy to operate, and low in manufacturing cost.

The invention discloses gentamycin JI-20B gene engineering bacterium, and construction and application thereof, and belongs to the technical field of biological medicine. Gentamycin JI-20B is directionally synthesized by constructing specific shuttle vector pFD605, utilizing molecular genetics technology to introduce micromonospora, accurately knocking out genB3 gene and genP gene in Micromonospora purpurea by means of frame deletion principle, and permanently inactivating the biological catalysis function of Micromonospora purpurea, utilizing a resistance marker to screen double-crossover mutant strain MicromonosporapurpureaB3P1220 (delta genB3P(gntJI)), performing fermentation on the mutant strain, extracting metabolite, performing structure analysis, and determining that the structure is anastomotic with that of prediction. The constructed engineering bacterium B3P1200 contains no any resistankt markers and the output of gentamycin JI-20B is stable.

The invention relates to a method for short-period fermentation production of low-impurity gentamicin by using micromonospora purpurea. The method comprises a spore slant medium, a primary seed medium, a secondary seed medium, a tertiary seed medium and a fermentation medium. The method is cheap in fermentative raw materials, short in fermentation period, high in yield and few in impurities, the problem that a downstream process is difficult to purify and low in yield is solved, and gentamicin sulfate meeting the requirements of the China Pharmacopoeia (2020 edition) is prepared.

The invention relates to a preparation method of a biological full-degradation kitchen garbage FDM microbial inoculum. The preparation method comprises the following steps: step 1, raw material preparation: culturing bacillus cereus fermentation liquor, aspergillus oryzae fermentation liquor, micromonospora purpurea fermentation liquor, candida krusei fermentation liquor and acetobacter aceti fermentation liquor, wherein the effective viable count in each single component in the microbial inoculum is high, the kitchen waste can be completely and quickly degraded only by a small amount of the microbial inoculum, the degradation rate is up to 96%, the amount of wastewater generated after the kitchen waste is fully degraded by the FDM microbial inoculum is small, granular precipitates are few, and up-to-standard discharge can be realized through a simple water treatment process.

The invention claims to protect gentamicin sulfate prepared through enhanced microbial fermentation, and an application method of the gentamicin sulfate. The enhanced microbial engineering bacterium is a Micromonospora purpurea C2 1591 strain which is obtained by screening through a normal-pressure room-temperature plasma mutagenesis technology, and the Micromonospora purpurea C2 1591 strain is registered and collected in the China General Microbiological Culture Collection Center (CGMCC for short, the address is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing) on June 13, 2021, and the collection number is CGMCC No.59450. The screened engineering bacterium for directionally producing gentamicin C2 in high yield can directionally produce the gentamicin C2 in the high yield, the component ratio of gentamicin C2 is increased to 85% or above from the original about 15%, and the highest fermentation titer of an optimized formula is 1184 U/mL through Plackett-Burman design and is increased by 42% than the titer of an original formula. Compared with an original strain, the yield of the gentamicin C2 is increased by three times, in addition, other isomers or impurities are hardly contained, the gentamicin C2 can be directly applied downstream, complex biotransformation and purification is not required, a large amount of cost is saved, and an industrialization requirement is met.

The invention relates to an Albizzia julibrissin straw compound fertilizer which is prepared from the following components: 1000 parts of dry straw, 1500 parts of biogas slurry, 200-300 parts of urea, 50-70 parts of potassium dihydrogen phosphate, 40-60 parts of boric acid, 40-50 parts of chromium trichloride, 50-80 parts of manganese chloride, 100-120 parts of zinc sulfate, 12-20 parts of Aspergillus flavus, 9-15 parts of Micromonospora and 20-30 parts of Polyangium cellulosum.

The invention relates to a manufacturing method of an Albizzia julibrissin straw compound fertilizer, which comprises the following steps: selecting biogas slurry which normally has produced gas for one month; fermenting, cutting up dry straws, putting into a fermentation tank together with the biogas slurry and potassium dihydrogen phosphate, simultaneously adding Micromonospora and Polyangium cellulosum, uniformly stirring, performing aerobic fermentation, and fermenting for 30 days while the temperature in the fermentation tank is kept at 35-45 DEG C; opening the fermentation tank, adding Aspergillus flavus and urea, uniformly stirring, closing the fermentation tank, performing anaerobic fermentation, keeping the temperature in the fermentation tank at 45-50 DEG C in the first three days, raising the temperature in the fermentation tank to 73 DEG C from the 3<rd> day to the 5 day, and keeping the temperature in the fermentation tank at 40-45 DEG C from the 6 day to the 30 day; and after the temperature in the fermentation tank falls to room temperature, adding boric acid, chromium trichloride, manganese chloride and zinc sulfate, uniformly mixing, bagging, and packaging.

The invention discloses biologically modified antibacterial hemp fiber and a preparation method thereof. The biologically modified antibacterial hemp fiber is prepared from raw materials in parts by weight as follows: 70-80 parts of hemp fiber, 5-10 parts of a microbial modifier and 0.1-3 parts of antibacterial components; the microbial modifier contains acidothermus, micromonospora and acetobacter xylinus; the antibacterial components are selected from one or more of subtilin, polymyxin E, gramicidin S and bacitracin. The biologically modified antibacterial hemp fiber is prepared from the antibacterial components and the hemp fiber through biochemical reaction of microorganisms and obtained by bonding connection of a large quantity of active groups, more antibacterial components are in bonding connection to the biologically modified antibacterial hemp fiber, and the biologically modified antibacterial hemp fiber is good in mechanical property and can be applied to every field of textiles and consumes.