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Microbial agent for promoting quick decomposition of agricultural wastes and preparation method

A technology for microbial inoculants and agricultural wastes, applied in the field of applied microorganisms, can solve the problems of large nutrient loss, poor composting quality, and long composting time, and achieve the effects of reducing nutrient loss, eliminating odorous gases, and promoting rapid ripening.

Inactive Publication Date: 2011-04-13
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Composting technology can convert agricultural waste into an important organic fertilizer resource, but the traditional composting method only uses the function of microorganisms in the compost itself, so there are problems such as long composting time, large nutrient loss, and poor composting quality. In order to effectively solve this problem, many microbial inoculum products have come out at home and abroad, such as enzyme bacteria and EM inoculum. These products alleviate the odor of organic waste to varying degrees, but the effect is not ideal

Method used

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  • Microbial agent for promoting quick decomposition of agricultural wastes and preparation method
  • Microbial agent for promoting quick decomposition of agricultural wastes and preparation method
  • Microbial agent for promoting quick decomposition of agricultural wastes and preparation method

Examples

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Effect test

Embodiment 1

[0041] The microbial inoculum is composed of Bacillus subtilis, Streptomyces microsporum, Trichoderma koningeni and Trichoderma globosa in parts by mass of 0.5:1.0:0.5:1.5, and the specific preparation method comprises the following steps:

[0042] 1) Medium:

[0043] 1.1) Potato agar medium: 200g of peeled potatoes, 20g of glucose, 15-20g of agar, sterilized in 15 pounds for 30 minutes;

[0044] 1.2) Nutrient gravy agar medium: peptone 10g, beef extract 3.0g, sodium chloride 10g, agar 15g, distilled water 1000ml, pH 7.0, sterilized at 15 pounds for 30 minutes;

[0045] 1.3) Carrier medium: rice husk 50%, bran 20%, bone meal 14%, fish meal 14%, brown sugar 2%;

[0046] 2) Primary expansion culture of microbial strains: Inoculate Trichoderma koningeni and Chaetomyces globosa on potato agar medium, and carry out constant temperature cultivation at 24°C for 7 days to obtain the primary species; Bacillus subtilis and Streptomyces microsporus Inoculate on nutrient broth agar medi...

Embodiment 2

[0051]The microbial bacterial agent is composed of Bacillus subtilis, Streptomyces microsporum, Trichoderma koningeni and Trichoderma globosa in parts by mass of 1:1:1:1, and the specific preparation method comprises the following steps:

[0052] 1) Medium:

[0053] 1.1) Potato agar medium: 200g of peeled potatoes, 20g of glucose, 15-20g of agar, sterilized in 15 pounds for 30 minutes;

[0054] 1.2) Nutrient gravy agar medium: peptone 10g, beef extract 3.0g, sodium chloride 10g, agar 15g, distilled water 1000ml, pH 7.0, sterilized at 15 pounds for 30 minutes;

[0055] 1.3) Carrier medium: rice husk 50%, bran 20%, bone meal 14%, fish meal 14%, brown sugar 2%;

[0056] 2) Primary expansion culture of microbial strains: Inoculate Trichoderma koningeni and Chaetomyces globosa on potato agar medium, and carry out constant temperature cultivation at 24°C for 7 days to obtain the primary species; Bacillus subtilis and Streptomyces microsporus Inoculate on nutrient broth agar medium...

Embodiment 3

[0061] The microbial bacterial agent is composed of Bacillus subtilis, Streptomyces microsporum, Trichoderma koningeni and Trichoderma globosa in a mass ratio of 1:0.5:1:1.5.

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Abstract

The invention belongs to the technical field of microbe application and discloses a microbial agent for promoting quick decomposition of agricultural wastes, prepared from the following steps of respectively carrying out first stage amplification culture and second stage amplification culture on four microbes which are bacillus subtilis, micromonospora streptomyces, trichoderma koningii and chaetomium globosum, and respectively obtaining four liquid cultures of the microbes; then commonly inoculating the four liquid cultures in a carrier culture medium according to a ratio with the inoculation amount of 0.2 percent, controlling the moisture content at 55-60 percent, culturing for 7 days at a constant temperature of 28 DEG C; and finally, carrying out low-temperature drying treatment on a complex bacteria subjected to culturing at the temperature of 35-40 DEG C, and carrying out live bacteria detection. The microorganism composting microbial agent can promote quick decomposition of agricultural waste aerobic composts, has remarkable action of eliminating malodorous gas generated by organic wastes and also reduces the nutrient loss caused by volatilization in a composting process.

Description

technical field [0001] The invention belongs to the technical field of applied microorganisms, and in particular relates to a microbial bacterial agent capable of promoting rapid decomposition of agricultural waste such as pig manure, chicken manure and wheat straw, and a preparation method thereof. Background technique [0002] With the rapid development of modern agriculture, especially the large-scale and intensive production of animal husbandry, large-scale centralized disposal of agricultural waste such as livestock and poultry manure has resulted. These organic wastes contain a large number of pathogenic microorganisms and parasite eggs , a large amount of malodorous gas will be produced during the disposal process, mainly including ammonia, hydrogen sulfide, indole, mercaptan, sulfide, formaldehyde, acetaldehyde, acrolein, methylamine, ethylamine, phenol, thiophenol and volatile Fatty acids, etc., pollute the human living environment and seriously endanger the health ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/14C12N1/20C05F11/08C12R1/125C12R1/465C12R1/885C12R1/645
Inventor 李鸣雷谷洁高华秦清军梁东丽
Owner NORTHWEST A & F UNIV
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