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Deodorizing compound inoculum for feces of livestock and poultry and preparation method of deodorizing compound inoculum

A technology of composite bacterial agent and poultry manure, which is applied in the direction of biochemical equipment and methods, chemical instruments and methods, and methods based on microorganisms, can solve the problems of poor adaptability of commercialized biological deodorants, high cost of chemical deodorization technology, Single effect and other problems, to achieve rapid and long-lasting deodorization effect, good ecological and economic benefits, and high number of viable bacteria

Active Publication Date: 2018-11-30
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem solved by the present invention is that the cost of chemical deodorization technology is high, the reaction is complex and produces secondary pollution, and the commercialized biological deodorant has poor adaptability and single effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Composition of deodorant compound bacteria agent:

[0031] Bacillus amyloliquefaciens CGMCC 1.857 (Bacillus amyloliquefaciens), 3.0×10 8 cfu / mL;

[0032] Alcaligenes CGMCC 1.3420 (Alcaligenes faecalis subsp.faecalis), 1.0×10 8 cfu / mL;

[0033]Arthrobacter humicola CGMCC 1.15654 (Arthrobacter humicola), 1.0×10 8 cfu / mL;

[0034] Streptomyces CGMCC 4.71 (Streptomyces levoris), 4.0×10 8 cfu / mL;

[0035] Micromonospora CGMCC 4.1152 (Micromonospora yulongensis), 3.0×10 8 cfu / mL.

[0036] The bacterial agent with the above composition is prepared by the following steps:

[0037] (1) Single strain fermentation: Bacillus amyloliquefaciens CGMCC 1.857 (Bacillus amyloliquefaciens), Alcaligenes CGMCC 1.3420 (Alcaligenes faecalis subsp.faecalis), Arthrobacter CGMCC 1.15654 (Arthrobacter humicola), Streptomyces CGMCC 4.71 (Streptomyces levoris) and small Five strains of CGMCC4.1152 (Micromonospora yulongensis) were fermented separately to obtain fermentation broth.

[0038...

Embodiment 2

[0052] Composition of deodorant compound bacteria agent:

[0053] Bacillus amyloliquefaciens CGMCC 1.857 (Bacillus amyloliquefaciens), 4.0×10 8 cfu / mL;

[0054] Alcaligenes CGMCC 1.3420 (Alcaligenes faecalis subsp.faecalis), 1.5×10 8 cfu / mL;

[0055] Arthrobacter humicola CGMCC 1.15654 (Arthrobacter humicola), 1.5×10 8 cfu / mL;

[0056] Streptomyces CGMCC 4.71 (Streptomyces levoris), 4.0×10 8 cfu / mL;

[0057] Micromonospora CGMCC 4.1152 (Micromonospora yulongensis), 5.0×10 8 cfu / mL.

[0058] The bacterial agent with the above composition is prepared by the following steps:

[0059] (1) Single strain fermentation: Bacillus amyloliquefaciens CGMCC 1.857 (Bacillus amyloliquefaciens), Alcaligenes CGMCC 1.3420 (Alcaligenes faecalis subsp.faecalis), Arthrobacter CGMCC 1.15654 (Arthrobacter humicola), Streptomyces CGMCC 4.71 (Streptomyces levoris) and Five strains of Micromonospora CGMCC4.1152 (Micromonospora yulongensis) were fermented respectively to obtain fermentation bro...

Embodiment 3

[0074] Composition of deodorant compound bacteria agent:

[0075]Bacillus amyloliquefaciens CGMCC 1.857 (Bacillus amyloliquefaciens), 5.0×10 8 cfu / mL;

[0076] Alcaligenes CGMCC 1.3420 (Alcaligenes faecalis subsp.faecalis), 2.0×10 8 cfu / mL;

[0077] Arthrobacter humicola CGMCC 1.15654 (Arthrobacter humicola), 2.0×10 8 cfu / mL;

[0078] Streptomyces CGMCC 4.71 (Streptomyces levoris), 5.0×10 8 cfu / mL;

[0079] Micromonospora CGMCC 4.1152 (Micromonospora yulongensis), 5.0×10 8 cfu / mL.

[0080] The bacterial agent with the above composition is prepared by the following steps:

[0081] (1) Single strain fermentation: Bacillus amyloliquefaciens CGMCC 1.857 (Bacillus amyloliquefaciens), Alcaligenes CGMCC 1.3420 (Alcaligenes faecalis subsp.faecalis), Arthrobacter CGMCC 1.15654 (Arthrobacter humicola), Streptomyces CGMCC 4.71 (Streptomyces levoris) and Five strains of Micromonospora CGMCC4.1152 (Micromonospora yulongensis) were fermented respectively to obtain fermentation brot...

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Abstract

The invention discloses a deodorizing compound inoculum for feces of livestock and poultry and a preparation method of the deodorizing compound inoculum, and relates to the field of microbe applications. The deodorizing compound inoculum and the preparation method are provided aiming at the problems that the technical cost for chemical deodorization is high, the reaction is complicated, secondarypollution is generated, and the commercial biological deodorant is poor in adaptation and single in function. The deodorizing compound inoculum for feces of livestock and poultry comprises five species of bacteria including bacillus amyloliquefaciens, Alcaligenes faecalis subsp.faecalis, Arthrobacter humicola, Streptomyces levoris and Micromonospora yulongensis. The preparation method of the deodorizing compound inoculum comprises the following steps: fermentation of single bacterial strains, preparation of the compound inoculum, and adding of a fresh culture medium, an activating agent and aprotection agent. The technical scheme has the beneficial effects that the treatment speed and utilization ratio for feces are improved, and the deodorizing effect is rapid and lasting.

Description

technical field [0001] The invention relates to the field of microorganism application, in particular to a compound microbial agent for deodorizing poultry manure and a preparation method thereof. Background technique [0002] With the continuous improvement of people's living standards, the demand for livestock and poultry products is also increasing. The Food and Agriculture Organization of the United Nations (FAO) predicts that the world's demand for meat products will increase by 73% by 2050. In order to meet such a rapidly growing demand for meat products, animal husbandry has gradually developed from traditional free-range farming to intensive and large-scale development, followed by a large amount of livestock and poultry manure that cannot be effectively treated and has become a major issue. sources of environmental pollution. Relevant statistics show that in 2010, the national production of livestock and poultry manure was about 4 billion tons, of which less than ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/38C02F11/02C12R1/07C12R1/05C12R1/06C12R1/465C12R1/29
CPCC12N1/20C12N1/38C02F11/02C02F2303/02
Inventor 黄胜威吴丽芳
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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