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A kind of utilization poultry manure to prepare composite microbial ecological bacterial fertilizer and method thereof

A technology of compound microorganisms and poultry manure, which is applied in the field of compound microbial ecological bacterial fertilizer by using poultry manure, can solve the problems of affecting environmental sanitation, polluting air, polluting water bodies, etc. Combine new effects

Active Publication Date: 2019-11-05
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large amount of poultry manure is not fully utilized, causing huge pollution, such as land occupation, soil pollution, water pollution, air pollution, environmental sanitation, etc., which has become a problem for production enterprises such as planting, breeding, and agricultural and sideline product processing, as well as governments at all levels. The problem

Method used

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  • A kind of utilization poultry manure to prepare composite microbial ecological bacterial fertilizer and method thereof
  • A kind of utilization poultry manure to prepare composite microbial ecological bacterial fertilizer and method thereof
  • A kind of utilization poultry manure to prepare composite microbial ecological bacterial fertilizer and method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The bacterial strain of the composite microbial bacterial fertilizer of the present embodiment constitutes as follows:

[0039] Bacillus subtilis CZ9, 1.0×108cfu / ml;

[0040] Pseudomonas putida P.putidacas8, 5.0×108cfu / ml;

[0041] Bacillus Lateraporus HF12, 1.0×108cfu / ml;

[0042] A. chroococcumcas1, 2.0×108 cfu / ml;

[0043] Yeast Sporobolomycesroseus CZ3, 1.5×108cfu / ml;

[0044] Streptococcus lactis CZ5, 3.0×108cfu / ml.

[0045]The preparation method of the present embodiment is as follows:

[0046] (1) Single strain fermentation: Bacillus subtilis CZ9, Bacillus Lateraporus HF12, A.chroococcumcas1, Pseudomonas putidacas8, Saccharomyces S.roseusCZ3, Streptococcus lactisCZ5 six strains Fermentation is carried out separately to obtain a fermentation broth.

[0047] The specific fermentation process is as follows:

[0048] Bacillus subtilis CZ9 and Bacillus Lateraporus HF12 were cultivated using LBG medium (glucose 50g, peptone 10g, yeast extract 5g, sodium chloride...

Embodiment 2

[0072] The bacterial strain of the composite microbial bacterial fertilizer of the present embodiment constitutes as follows:

[0073] Bacillus subtilis CZ9, 2.0×108cfu / ml;

[0074] Pseudomonas putida P.putidacas8, 7.0×108cfu / ml;

[0075] Bacillus Lateraporus HF12, 1.5×108cfu / ml;

[0076] A. chroococcumcas1, 2.5×108 cfu / ml;

[0077] Yeast Sporobolomycesroseus CZ3, 2.0×108cfu / ml;

[0078] Streptococcus lactis CZ5, 4.0×108cfu / ml.

[0079] The preparation method of the present embodiment is as follows:

[0080] (1) Single-strain fermentation: Bacillus subtilis CZ9, Bacillus Lateraporus HF12, A.chroococcumcas1, Pseudomonas putidacas8, Saccharomyces sporobolomycesroseus CZ3, and Streptococcus lactis CZ5 were separately Fermentation is carried out to obtain a fermentation broth.

[0081] Bacillus subtilis CZ9 and Bacillus Lateraporus HF12 were cultivated using LBG medium (glucose 50g, peptone 10g, yeast extract 5g, sodium chloride 10g, pure water 1000ml); the inoculum size was...

Embodiment 3

[0104] The bacterial strain of the composite microbial bacterial fertilizer of the present embodiment constitutes as follows:

[0105] Bacillus subtilis B.subtilisCZ9, 3.0×108cfu / ml;

[0106] Pseudomonas putida P.putidacas8, 8.0×108cfu / ml;

[0107] Bacillus Lateraporus HF12, 2.0×108cfu / ml;

[0108] A. chroococcumcas1, 3.0×108 cfu / ml;

[0109] Saccharomyces Sporobolomycesroseus CZ3, 3.0×108cfu / ml;

[0110] Streptococcus lactis CZ5, 5.0×108cfu / ml.

[0111] The preparation method of the present embodiment is as follows:

[0112] (1) Single-strain fermentation: Bacillus subtilis CZ9, Bacillus Lateraporus HF12, A.chroococcumcas1, Pseudomonas putidacas8, Saccharomyces sporobolomycesroseus CZ3, and Streptococcus lactis CZ5 were separately Fermentation is carried out to obtain a fermentation broth.

[0113] Bacillus subtilis CZ9 and Bacillus Lateraporus HF12 were cultured using LBG medium (glucose 50g, peptone 10g, yeast extract 5g, sodium chloride 10g, pure water 1000ml); the i...

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Abstract

The invention discloses a method and method for preparing compound microbial ecological fertilizer by using poultry manure. The nitrogen-fixing bacteria and Pseudomonas putida isolated from soil and pig manure are subjected to nitrogen ion implantation mutagenesis, and highly active mutations are screened. Bacterial strains: separately ferment six strains of Bacillus subtilis, Bacillus lateralosporus, Azotobacter torus, Pseudomonas putida, yeast, and Streptococcus lactis to obtain fermentation broth; according to 1~3:5~8: 1~2: 2~3: 1.5~3: 3~5 volume ratio is mixed, and the composite bacterial liquid is prepared, and the fresh culture substrate of sugarcane molasses is added to the above-mentioned composite bacterial agent according to the mass concentration of 2 to 5%; according to the mass concentration 2~2.5‰ Add potassium sorbate to the compound bacterial solution, stir evenly; add 0.4~1g / L attapulgite, stir well, and the active bacterial agent is obtained; put the above active bacterial agent into the septic tank, wait for the feces After the decomposition process is completed, dry and wet are separated, and the solid part is the compound microbial fertilizer.

Description

technical field [0001] The invention relates to a microbial fertilizer, in particular to a method for preparing compound microbial ecological bacterial fertilizer by using poultry manure. Background technique [0002] With the development of my country's livestock and poultry breeding industry and the implementation of the "Vegetable Basket Project", the large-scale and intensive livestock and poultry breeding industry has risen rapidly, and the production of livestock and poultry manure has increased year by year. A large amount of poultry manure is not fully utilized, causing huge pollution, such as land occupation, soil pollution, water pollution, air pollution, environmental sanitation, etc., which has become a problem for production enterprises such as planting, breeding, and agricultural and sideline product processing, as well as governments at all levels. The problem. Therefore, realizing the resource utilization of poultry and livestock manure is one of the importa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05F3/00C05F17/00C12N15/01C12N1/20C12R1/065C12R1/40C12R1/125C12R1/07C12R1/645C12R1/46
CPCC05F3/00C05F17/00C12N1/16C12N1/20C12N15/01C12N1/205C12R2001/065C12R2001/40C05F11/08Y02E50/30Y02W30/40Y02A40/20
Inventor 吴丽芳黄胜威高战元潘高志李明浩赵薇薇陈雪蔡冬清吴正岩
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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