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A kind of corn stalk fermentation microbial bacterial agent and its preparation method and fermentation method

A technology for fermenting microorganisms and corn stalks, applied in the field of microbial fermentation, can solve the problems of low total degradation rate of cellulose, weak decomposition ability, long fermentation period, etc., and achieve the effects of reducing the decomposing time, strong enzyme activity, and accelerating straw fermentation.

Active Publication Date: 2015-09-30
TIANJIN OCEAN PAL CAROL BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the deficiencies of existing products, provide a corn stalk fermentation microbial inoculum and its preparation method, and overcome the single type of microbial inoculum used in the original straw fermentation and degradation process, weak decomposition ability, total cellulose Problems related to low degradation rate and long fermentation cycle

Method used

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  • A kind of corn stalk fermentation microbial bacterial agent and its preparation method and fermentation method
  • A kind of corn stalk fermentation microbial bacterial agent and its preparation method and fermentation method
  • A kind of corn stalk fermentation microbial bacterial agent and its preparation method and fermentation method

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Embodiment 1

[0031] The invention relates to a corn stalk fermenting microbial bacterial agent, which is composed of Phanerochaete chrysosporium, Trichoderma korningii, Bacillus subtilis, Bacillus licheniformis and Candida tropicalis.

[0032] The volume fraction of each component of the corn stalk fermentation microbial agent is:

[0033]

[0034] The preparation method of said a kind of corn stalk fermenting microbial inoculant comprises the steps of:

[0035] Step 1: the Phanerochaete chrysosporium, Trichoderma koningeni, Bacillus subtilis, Bacillus licheniformis, and Candida tropicalis were respectively inoculated into corresponding culture media for activation and culture to obtain first-generation strains respectively;

[0036] Step 2: Inoculate the five first-generation bacterial strains obtained in step 1 into nutrient broth agar medium separately to obtain five bacterial solutions;

[0037] Step 3: The five bacterial solutions obtained in step 2 were mixed in equal proportions...

Embodiment 2

[0049] The invention relates to a corn stalk fermenting microbial bacterial agent, which is composed of Phanerochaete chrysosporium, Trichoderma korningii, Bacillus subtilis, Bacillus licheniformis and Candida tropicalis.

[0050] The volume fraction of each component of the corn stalk fermentation microbial agent is:

[0051]

[0052] The preparation method of said a kind of corn stalk fermenting microbial inoculant comprises the steps of:

[0053] Step 1: the Phanerochaete chrysosporium, Trichoderma koningeni, Bacillus subtilis, Bacillus licheniformis, and Candida tropicalis were respectively inoculated into corresponding culture media for activation and culture to obtain first-generation strains respectively;

[0054] Step 2: Inoculate the five first-generation bacterial strains obtained in step 1 into nutrient broth agar medium separately to obtain five bacterial solutions;

[0055] Step 3: The five bacterial solutions obtained in step 2 were mixed in equal proportions...

Embodiment 3

[0067] The invention relates to a corn stalk fermenting microbial bacterial agent, which is composed of Phanerochaete chrysosporium, Trichoderma korningii, Bacillus subtilis, Bacillus licheniformis and Candida tropicalis.

[0068] The volume fraction of each component of the corn stalk fermentation microbial agent is:

[0069]

[0070]

[0071] The preparation method of said a kind of corn stalk fermenting microbial inoculant comprises the steps of:

[0072] Step 1: the Phanerochaete chrysosporium, Trichoderma koningeni, Bacillus subtilis, Bacillus licheniformis, and Candida tropicalis were respectively inoculated into corresponding culture media for activation and culture to obtain first-generation strains respectively;

[0073] Step 2: Inoculate the five first-generation bacterial strains obtained in step 1 into nutrient broth agar medium separately to obtain five bacterial solutions;

[0074] Step 3: The five bacterial solutions obtained in step 2 were mixed in equal...

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Abstract

The invention discloses a corn straw fermentation microorganism agent which consists of phanerochaete chrysosporium, trichoderma koningii, bacillus subtilis, bacillus licheniformis and monilia tropicalis. The corn straw fermentation microorganism agent disclosed by the invention has the beneficial effects that the microorganism agent is reasonable in formula and convenient to use; the straw rotting speed is high; the corn straw fermentation microorganism agent has the characteristics of new combination, stable structure, high enzyme activity, high microbial content and the like. The corn straw fermentation microorganism agent can be used for performing high-efficiency and environment-friendly fermentation on corn straw materials, so that a feed can contain a large amount of microorganism live cells, clastic enzymes and other active components, and the utilization rate of the corn straw materials can be effectively increased. The practical significance of solving the problem of environment pollution is achieved; furthermore, the actual application values such as the protein content and the nutritional value in the corn straw materials can be improved; good preconditions are created for recycling application of corn straws.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a corn stalk fermentation microbial inoculant and a preparation method and a fermentation method thereof. Background technique [0002] The fibrous material produced by photosynthesis is extremely rich in the world every year, and the annual output of crop straw in my country reaches 500 million tons. Among them, except for a very small part of straw that is used as feed for ruminants such as cattle and sheep or returned to the field as fertilizer, most of it is burned and discarded. The combustion value of crop straw is very low, only 4200 cal / g, only 32% of methane gas, and 55% of ethanol. Therefore, it is very wasteful to directly use it as fuel, and it also pollutes the environment. And directly used as feed, because the cell wall composed of lignin, cellulose and hemicellulose is difficult to be digested by gastric juice, so the digestibility is not good. Therefore, t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14C12N1/16C05F11/08C05F17/00C12R1/645C12R1/885C12R1/125C12R1/10C12R1/74
CPCY02W30/43Y02W30/40
Inventor 郎明远赵大虎周鹏
Owner TIANJIN OCEAN PAL CAROL BIOTECH
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