60results about How to "High bacterial count" patented technology

The invention discloses an active microbial agent for deodorizing livestock and poultry feces. The active microbial agent comprises six types of strains, namely bacillus subtilis, bacillus cereus, arthrobacterium, streptomycete, saccharomycetes and lactic streptococci. The invention further discloses a preparation method of the active microbial agent for deodorizing livestock and poultry feces. The active microbial agent for deodorizing livestock and poultry feces and the preparation method thereof utilize synergism of the six types of feces degrading bacteria or deodorization bacteria and have the advantages of being novel in strain combination, stable in structure, great in enzyme activity, large in bacteria number and the like, meanwhile, the problems that in traditional compost treatment of livestock and poultry feces, feces-urine separation is required, feces degradation is low in speed, and the deodorizing effect is not obvious are solved, and the livestock and poultry feces treatment speed and utilization rate are raised.

The invention provides to a microorganism-containing fertilizer produced by a mycoderm method and a production method of the fertilizer. In the prior art, microorganism bactericides, organic matters, chemical fertilizer and the like are mixed according to a preset formula, and the mixture is subjected to granulation, high-temperature drying, screen separation, cooling and the like so as to produce a finished product. In the production method provided by the invention, the fertilizer without bactericides is granulated, dried, screened and cooled; active bacterium powder is coated on the outer surface of the fertilizer particles at the tail end of the production line by using a film technology; and the microbial content of the whole fertilizer when in detection reaches the technical standard of national regulation so that a film fertilizer is formed. The method provided by the invention is used for producing the fertilizer with active bacterium.

The present invention utilizes the crop waste material straw and stalk as raw material and adopts the bacillus, photosynthetic bacterium, microzyme, Aspergillus oryzae and seed liquor of basidiomycetes as beneficial microbial pool, and makes inoculation at room temp. to make the straw and stalk undergo the process of solid fermentation so as to obtain microbial fermented feed.

The invention discloses a method for producing microbial fermentation wet feed by utilizing beer yeast paste. Bean pulp, corn slurry dry powder, corn germ meal, wheat bran and rice bran serve as solid fermentation raw materials, live yeast in the beer yeast paste serves as a main strain, bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus and lactobacillus plantarum serve as auxiliary strains, and the microbial fermentation wet feed is produced. Beer yeast paste seed solution and seed solution of four bacteria are mixed and added into the solid fermentation raw materials, fully stirred and placed in an anaerobic container to be contained and fermented or placed on a fermentation bed to be stacked and fermented; through the fermentation process for 96-120 h, the mixture directly serves as feed or is dried at a low temperature of 60 DEG C and then smashed to 60 meshes through a pulverizer, and the microbial fermentation wet feed produced by utilizing the beer yeast paste is obtained. The produced microbial fermentation wet feed is simple in production process and low in cost, saves energy, is environmentally friendly and good in product quality, and obviously promotes growth of bred animals.

The invention discloses a method of producing edible immobilized active probiotics, comprising the following steps: adding one kind of or various kinds of mature probiotics strains to immobilized carrier solution which is suitable for the growth and the propagation of the probiotics strains according to a certain proportion; adding dropwise the immobilized carrier solution which comprises one kind of or various kinds of probiotics strains into immobilized solution, or adding the immobilized solution to immobilized carrier to solidify the immobilized carrier. The solidified immobilized carrier which comprises one kind of or various kinds of probiotics strains is washed by sterile water and then is sealed to be cultured for 8-72 hours at 10-45 DEG C. The produced immobilized probiotics do not need to be separated and frozen to dry, can be taken directly as probiotics and can be put in other food to take. If being sealed to store at normal temperature for a long time, the immobilized probiotics can grow without stop.

The invention discloses a preparation method of micro-storage straw feed, which comprises the following steps of: a, activating an EM (Effective Microorganism) probiotic stock solution to obtain a bacterium solution, b, diluting the activated bacterium solution to obtain a fermentation liquid, c, placing straw in a sealed fermentation tank, adding the fermentation liquid obtained in Step b uniformly for anaerobic fermentation for 3-7 days, d, adding soyabean meal and brewery mash to the micro-storage straw obtained through fermentation in Step c, and uniformly mixing, and e, drying and pelletizing a mixture obtained in Step d, and obtaining the feed, wherein the addition amount of the fermentation liquid accounts for 50-70% of the total amount of the straw; the addition amount of the soyabean meal accounts for 8-15% of the total amount of the straw; and the addition amount of the brewery mash accounts for 15-25% of the total amount of the straw. The micro-storage straw feed prepared by the preparation method of the micro-storage straw feed has the characteristics of low breeding cost, high nutritive value and edible safety.

The invention relates to a microbe biological agent, and concretely relates to a production method of a bacillus subtilis biological agent used for a sodium humate-containing feed in a medium. The bacillus subtilis bacterial strain is subjected to first-stage solid seed culture and second-stage liquid seed fermentation culture to obtain a bacterial classification liquid. The bacterial classification liquid is inoculated in the medium which takes glucose, sodium humate, soybean meal, corn and wheat bran as raw materials for three-stage solid aerobic fermentation, the culture condition is adjusted to form spore, the spore can be treated, dried, and crushed to produce the bacillus subtilis biological agent. The biological agent contains more than or equal to 200 hundreds million/g of acillus subtilis, the pH value is 5.6-6.6, the moisture is less than or equal to 10%, and the granularity is greater than or equal to 100 meshes, the color presents special shallow grey or grey black, and the shelf life is 18 months. The biological agent can be taken as an additive application in aquatic product, and livestock and poultry culture, can increase the production performance and improve the culture environment.

The invention discloses a salmonella pullorum SP9905. The strain is preserved at China Center for Type Culture Collection with an address of Wuhan University on October 15, 2014 and the preservation number is CCTCC NO.M 2014478. The invention also discloses an application of the salmonella pullorum in preparation of a live vaccine. The vaccine provided by using salmonella pullorum SP9905 has the advantages of high viable count and low freeze-drying death rate; the product is safe and reliable; a strong protecting force can be generated in the fourteenth day due to an immune protection effect, the immune protective rate is 100% and a good protective capability (80-100%) can be obtained for the other serotype salmonella (salmonella enteritidis and salmonella gallinarum).

The invention discloses a method for producing living paratyphoid vaccine for piglets and a product thereof. The method comprises the following steps: (1) preparing primary strains; (2) preparing secondary strains; and (3) culturing bacterial liquid: inoculating the secondary strains into a culture medium for culturing, after culturing, immediately adding a stabilizing agent which is sterilized and preheated to 37 DEG C to ensure that the vaccine contains 1.5 percent of gelatin and 5 percent of sucrose, and mixing uniformly to obtain the bacterial liquid, wherein the culture medium in the step (3) consists of a common broth medium and a synthetic medium in the volume ratio of 2:1. In the living paratyphoid vaccine for piglets produced by the method, the cultured bacteria count is over 50 billion per milliliter; the maximum cultured bacteria count is 58 billion per milliliter; and the freeze-drying death rate is reduced to 20-40 percent. The method for producing the living paratyphoid vaccine for the piglets has the advantages of high bacterial count of the living vaccine, low freeze-drying death rate, easy production, high yield, low production cost, and the like.

Provided is automatic vending equipment for liquid fertilizer, comprising a cabinet in which are arranged a central control unit, a vending apparatus, a stock storage tank, an immersion pump and an extraction apparatus. The vending apparatus is electrically connected with the central control unit, and can accept purchase commands from users and transmit the commands to the central control unit. The stock storage tank is used to accommodate the liquid fertilizer. The immersion pump is arranged in the stock storage tank and electrically connected to the central control unit, so as to accept commands from the central control unit and drive the liquid fertilizer in the stock storage tank to circulate and convect up and down. The extraction apparatus is electrically connected with the central control unit and can accept commands from the central control unit to extract the liquid fertilizer from the stock chest storage tank.

The invention discloses a preparation method of a straw micro-storage feed, and is characterized in that the preparation method includes the following steps: (1) constructing a micro-storage cellar; (2) carrying out raw material pretreatment comprising sun-drying straw for 3-5 h to make the water content be 60%-70%, and cutting short into 2-3 cm; (3) preparing a fermentation agent: (4) weighing table salt according to 0.3% (weight percentage) of the straw dry basis amount, and evenly mixing into a fermentation bacterial powder; (5) laying a plastic film in the cellar, allowing the plastic film length to be 1-1.5 m higher than that the periphery of a cellar mouth; (6) sprinkling the crushed straw with the thickness of 20-30 cm at the bottom of the cellar, then sprinkling the prepared fermentation bacterial powder on the straw, and treading down with feet; and (7) allowing the micro-storage anaerobic fermentation time to be 7-8 days in summer, 10-12 days in spring and in autumn and 13-15 days in winter, checking whether the feed has a wine fragrance, and allowing for use. The nutritional value of the straw feed can be improved, and the feed has relatively high content of digestible proteins and has good palatability.

The invention discloses a compound bacterial agent applied to straw mulching. The compound bacterial agent comprises 1.0*10<9>cfu/g-8.0*10<9>cfu/g of Bacillus sp.CZ5, 1.0*10<8>cfu/g-6.0*10<9>cfu/g of Arthrobacter sp.WH16, 1.0*10<9>cfu/g-2.0*10<10>cfu/g of Streptomyces sp.WH36 and 1.0*10<7>cfu/g-5.0*10<8>cfu/g of Micromonospora sp.WH13. The invention further provides a preparation method of the compound bacterial agent applied to straw mulching. Compared with the prior art, the compound bacterial agent applied to straw mulching has the advantage that purposes of quick decomposition of straw, suppression of soil pathogens and increase of crop yield are achieved under synergistic effect of various straw degradation bacteria/biocontrol bacteria.

The invention relates to the technical field of fermentation culture, in particular to a pseudomonas aeruginosa fermentation medium, a fermentation culture method thereof and a vaccine preparation method. The fermentation medium comprises peptone, glucose, yeast powder, sodium chloride, Na2HPO4, KH2PO4, MgSO4, sodium citrate, urea and decavitamin B. The fermentation medium is suitable for high-density fermentation and growth of pseudomona aeruginosa, and the viable count of pseudomonas aeruginosa can be greatly increased; compared with an original traditional medium, the improved medium prolongs the logarithmic phase from 4-8 h to 4-10 h remarkably and increases the microbial content from 14-15.9 billion to 24-25 billion, the pH (potential of hydrogen) value is kept at 7.0, and the yield of pseudomonas aeruginosa is greatly increased.

The invention relates to the technical field of a clostridium butyricum fermentation process, in particular to a multi-stage fermentation production method for highly yielding spore clostridium butyricum. The method comprises the steps of seed liquid preparation, primary oxygen fermentation culture, secondary fermentation culture and adsorption drying, wherein glucose is supplemented in the secondary fermentation process; the temperature and the pH value are controlled in the whole process; the low-speed stirring fermentation culture is performed in the whole process; the dissolved oxygen riskis eliminated. The spore formation rate is a hundred percent; the microbial content of the clostridium butyricum of the bacterium liquid is high; the spore loss rate of the clostridium butyricum is low; the multi-stage fermentation is more favorable for the high-temperature resistance of the spores; the microbial content in the bacterium liquid is 2.5 to 3 billion/ml; the heat loss of the bacterium powder in the adsorption drying process is improved; the live spore number of clostridium butyricum in the bacterium powder is improved by 1 to 1.5 times; the microbial content in the bacterium powder reaches 0.4 to 0.5 billion/g; the live spore number and the butyrate content in the bacterium powder are improved; the product quality and the competitive power are improved; the obvious economicbenefits are realized.

The invention discloses a method for high density culture of a strain of lactobacillus fermentum La-Y1. The method comprises that: (1) fermentation conditions are a culture temperature of 37 DEG C, an initial pH value of 6.0, and an inoculation amount of the lactobacillus fermentum La-Y1 of 5%; and (2) fermentation medium components comprise, by weight, 2 parts of glucose, 0.5 part of beef extract, 0.5 part of casein peptone, 2 parts of corn steep powder, 0.2 part of ammonium citrate dibasic, 0.5 part of sodium acetate, 0.1 part of tween 80, 0.025 part of manganese sulfate, 0.058 part of magnesium sulfate, and 0.2 part of potassium dihydrogen phosphate. The present invention further discloses an application method of the lactobacillus fermentum La-Y1. With the present invention, the number of the lactobacillus fermentum La-Y1 in the culture medium of the present invention is relatively increased by more than 10 times compared with the number of the lactobacillus fermentum La-Y1 in a basic MRS culture medium, wherein the bacterial concentration is 3.05*10<10> cfu/mL. According to the present invention, beef extract content and casein peptone content in the culture medium of the present invention are only 0.5% so as to substantially reduce cost compared to the basic MRS culture medium, and realistic significances are provided for development of direct vat set starters.

The invention discloses a compound preparation containing clostridium butyricum and pediococcus acidilactici as well as a preparation method and application of the compound preparation. The compound preparation comprises clostridium butyricum with the bacterium content of 5-6*10<8> CFU/mL and pediococcus acidilactici with the bacterium content of 5.0-6.0*10<9> CFU/mL, wherein the clostridium butyricum adopts clostridium butyricum GBW-N1 with the preservation number of CGMCC No. 14499. The compound preparation is prepared by inoculating two strains in a staggered manner and carrying out fermentation culture in a designed fermentation culture medium for 16-22 hours, and fermentation liquor can be used after being filled. The compound preparation can well improve the weight gain rate, the survival rate and the growth performance of aquatic animals, is beneficial to improvement of aquaculture income, is good in stability and convenient to store, and thus has wide application value.

The invention provides a swine pasteurellosis vaccine enrichment medium, and relates to the field of biology. The swine pasteurellosis vaccine enrichment medium is prepared from the following raw materials by weight: 20.0g of meat liver and stomach membrane digestive juice powder, 8.0g of bovine pancreas digestive juice, 10.0g of peptone, 5.0g of NaCl, 1.0g of a growth promoting factor, and purified water added to 1000ml. A preparation method of the swine pasteurellosis vaccine enrichment medium comprises the following steps: adding all the raw materials for preparing the swine pasteurellosisvaccine enrichment medium into purified water, fully dissolving the raw materials, and carrying out sterilization at 121 DEG C for 15 minutes for later use. The invention relates to an application ofa synthetic dry powder culture medium in swine pasteurellosis vaccine production. The synthetic dry powder culture medium has the beneficial effects that commercial raw materials can be selected, theculture medium is clear, the number of cultured bacteria is high, and the freeze-drying survival rate of the bacteria is increased.

The invention relates to a movable fermentation method for preparing a wet-state yeast culture by using rice husk removed white wine vinasse as raw materials. The movable fermentation method comprises the following steps: removing rice husks from white wine vinasse, compounding materials, activating strains, performing inoculation and mixing, controlling temperature for fermentation, performing enzymolysis for breaking walls, and postprocessing products, wherein the step of postprocessing products comprises the steps of inspecting products, packaging inspected products and obtaining finished products. According to the movable fermentation method disclosed by the invention, a mechanical method is adopted for separating the rice husks, the content of crude fibers in the products is reduced, the nutrient value of the products is increased, and the application range of the products is extended. The rice husks separated from the white wine vinasse are mixed with coal to be used as boiler fuel, and a heat source is provided for warming the products, so that the cost of heating power is reduced, comprehensive utilization is realized, and the pollution is not caused. A bacteria-enzyme synergistic fermenting enzymolysis technology combining microzyme, protease and cellulose is adopted, so that the stability of the technology is good, mold pollution is not caused, and the products are safe. A temperature control technology by a two-step method is adopted, so that the cultured yeast count is high, the enzymolysis is thorough, the wall breaking of the yeast is sufficient, and the nutrient value is higher.

The invention provides a preparation method and an application of an organic material decomposing agent. According to the organic material decomposing agent, a microbial composite strain composed of various functional microorganisms is mainly taken as a strain, and is subjected to liquid-solid multi-stage comprehensive fermentation, and comprehensively applied and transformed into the organic material decomposing agent, wherein the weight ratio of a clostridium butyricum strain, a saccharomyces cerevisiae strain, a lactobacillus plantarum strain and a bacillus subtilis strain is (10-15): (20-25): (10-20): (35-40); and product production simulates the process of compost heating-high temperature-cooling. When the organic material decomposing agent acts on livestock and poultry manure compost, the fermentation temperature rises rapidly, the highest temperature of compost is 76.5 DEG C, the high temperature period lasts for 12-16 days, the use is highly compatible with the microbial evolution process of natural compost, and functional bacteria reproduce rapidly to be dominant and play a role; and due to in-situ fermentation, a large number of proteases, cellulases and amylases are produced in the fermentation process to form a stable and efficient micro-ecosystem, which has a large number of bacteria and high enzyme activity, and functional bacteria and enzyme substances act together to rapidly achieve harmless treatment of livestock and poultry manure.

The invention discloses a petroleum hydrocarbon degrading bacterium and a fermentation method thereof. The petroleum hydrocarbon degrading bacterium is brevibacillus parabrevis with strain name of GWN-2, and is preserved in China General Microbiological Culture Collection Center with preservation number of CGMCC No. 13903. The degrading bacterium is characterized in that the gene sequence is shownin a sequence table. After inoculation and proliferation culture of the petroleum hydrocarbon degrading bacterium, a petroleum hydrocarbon degrading bacterium product capable of degrading oily sludgeis obtained, the oil content is reduced by about 80% after the oily sludge is treated for 20 days, and a novel approach is provided for treatment of the oily sludge in oil fields.