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Salmonella pullorum SP9905 and application thereof

A technology of Salmonella and pullorum, applied in the field of bioengineering, can solve the problems of increased mortality, abuse or waste, selection of drug types, administration routes, methods, dosage and maintenance time confusion, etc., and achieve low freeze-drying mortality , high bacterial count, product safety and reliability

Active Publication Date: 2015-07-15
JIANGSU INST OF POULTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pullorum is ubiquitous in chicken farms in my country, manifested in increased mortality in the brooding and rearing stages, causing serious economic losses to producers
Domestic control of pullorum pullorum usually uses antibacterial drugs to reduce its harm, but the selection of drug types, routes of administration, methods, doses and maintenance time is relatively confusing, there is a considerable degree of blindness, and abuse or waste is very common
At the same time, long-term repeated medication will not only increase the cost of feeding, but also lead to a series of problems such as enhanced bacterial resistance and drug residues in poultry products.
Facts have proved that only relying on drug control is not a good way to prevent pullorum

Method used

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  • Salmonella pullorum SP9905 and application thereof
  • Salmonella pullorum SP9905 and application thereof
  • Salmonella pullorum SP9905 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1) Separation, purification and staining microscopic examination

[0020] Salmonella pullorum SP9905 strain was isolated from the caecal contents of the 11-month-old Langshan chicken in a chicken farm in Yangzhou City, Jiangsu Province in May 1999. The specific separation method is: aseptically collect the contents of the cecum and add it to the selenite cystine enrichment solution to incubate overnight at 37°C, then inoculate MacConkey agar and incubate at 37°C for 24 hours, with a diameter of 0.5mm-1.0mm. The off-white transparent colony with smooth surface was inoculated on Martin agar medium and cultivated at 37°C for 24 hours. The purely cultured bacteria were subjected to Gram staining microscopic examination, and then observed with an optical microscope, and they showed elongated bacilli with slightly rounded ends.

[0021] 2) Biochemical identification

[0022] Pick colonies from Martin agar plates for biochemical identification tests. The micro biochemical identifi...

Embodiment 2

[0040] After rejuvenating the strain of Salmonella pullorum SP9905, a typical colony was selected for expansion and cultured to make a seed bacterial solution. Inoculate 2.5% of the total amount of the culture medium into the liquid culture medium, collect the bacterial liquid after deep aeration culture at 37°C for 20 hours, centrifuge at 4000r / min for 15 minutes to remove the supernatant, and immediately add the bacterial mud to the sterilized freeze-dried protective agent Mix well, and then vacuum freeze-dry to make a live vaccine of Salmonella pullorum. The test results showed that each milliliter of culture medium contained 44.876 billion live bacteria, and the freeze-drying mortality rate was 14.9%.

Embodiment 3

[0042] After rejuvenating the strain of Salmonella pullorum SP9905, a typical colony was selected for expansion and cultured to make a seed bacterial solution. The seed bacteria solution was inoculated into the liquid culture medium at 5% of the total culture medium, and the bacteria solution was collected after 18 hours of deep aeration culture at 37°C, centrifuged at 4000r / min for 15 minutes to remove the supernatant, and the bacteria mud was immediately added to the sterilized freeze-dried protective agent Mix well, and then vacuum freeze-dry to make a live vaccine of Salmonella pullorum. The test results show that each milliliter of culture medium contains 51.743 billion live bacteria, and the freeze-drying mortality rate is 22.5%.

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Abstract

The invention discloses a salmonella pullorum SP9905. The strain is preserved at China Center for Type Culture Collection with an address of Wuhan University on October 15, 2014 and the preservation number is CCTCC NO.M 2014478. The invention also discloses an application of the salmonella pullorum in preparation of a live vaccine. The vaccine provided by using salmonella pullorum SP9905 has the advantages of high viable count and low freeze-drying death rate; the product is safe and reliable; a strong protecting force can be generated in the fourteenth day due to an immune protection effect, the immune protective rate is 100% and a good protective capability (80-100%) can be obtained for the other serotype salmonella (salmonella enteritidis and salmonella gallinarum).

Description

Technical field [0001] The invention belongs to the technical field of bioengineering, and specifically relates to a Salmonella pullorum SP9905, and its application and preparation method in the preparation of live vaccines. Background technique [0002] Pullorum is a common multiple infectious disease in poultry caused by Salmonella pullorum (Salmonella pullorum). Pullorum is common in chicken farms in my country, and it is manifested in the increase in mortality during the brooding and rearing stages, causing serious economic losses to producers. The domestic control of pullorum disease usually uses antibacterial drugs to reduce its harm, but the choice of drug type, route of administration, method, dosage and maintenance time is rather chaotic, there is a considerable degree of blindness, and abuse or waste is very common. At the same time, repeated use of drugs for a long time not only increases the cost of feeding, but also leads to a series of problems such as enhanced bac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K39/112A61P31/04A61P1/12C12R1/42
Inventor 龚建森苗晋锋许明窦新红张笛朱春红顾蓓蓓刘学贤徐步童海兵邹剑敏
Owner JIANGSU INST OF POULTRY SCI
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