502 results about "Clostridiales" patented technology

The invention relates to the technical field of biological pharmacy, and discloses a probiotic microcapsule with a function of improving intestinal microecology, which comprises the following components in percentage by weight: lactobacillus rhamnosus: each gram of formula contains 1010 CFU of lactobacillus rhamnosus; lactobacillus acidophilus: each gram of the formula contains 109 CFU; clostridium butyricum: each gram of the formula contains 108 CFU; 5%-8% of a nano sodium alginate-chitosan compound; 3%-5% of peptide nanoparticles; 2%-4% of a natural polymer intelligent response film; 3%-6% of arabinoxylan oligosaccharide; 4%-7% of resistant dextran; 2%-4% of lentinan; and 0.1%-0.3% of resveratrol. Through a double-layer or three-layer embedding technology of the nano sodium alginate-chitosan compound and the intelligent response polymer film, the tolerance of the probiotics in gastric acid and cholate environments is remarkably enhanced, and targeted release of the probiotics in small intestines and colon is ensured.

The invention discloses a probiotic-polyphenol nanoparticle colon-targeted co-delivery system as well as a preparation method and application thereof, and belongs to the field of biological medicines. The FCPs and hyaluronic acid (HA) are combined through non-covalent interaction, so that the hydrogel has colon targeting property and mucosal adhesion at the same time, and delivery of probiotics is facilitated. The hydrogel (HF) based on polysaccharide has good biocompatibility, and can be used for effectively encapsulating the probiotics and the natural products. Then, the PDA-TH NPs and BA are incorporated into the polysaccharide chain network of HF, and finally the BA-HF-PDAT targeted co-delivery system is constructed. The BA (at) HF-PDAT targeted co-delivery system can protect BA from serious gastrointestinal stress, and is jointly assembled with PDT-TH NPs to realize dual slow release of thymol (Thy), so that the treatment effect of BA and Thy is improved, and the BA (at) HF-PDAT targeted co-delivery system contributes to treatment of clostridium difficile infection (CDI).

The invention discloses an efficient denitrification method and system for landfill leachate, and the method comprises the following steps: diluting waste molasses, and then adding a compound enzyme preparation containing cellulase, pectinase and alpha-amylase for enzymolysis pretreatment to obtain a pretreatment solution; inoculating the pretreatment liquid with a compound flora containing clostridium butyricum and succinic acid-producing anaerobic spirillum, carrying out anaerobic fermentation, and separating to obtain a liquid carbon source containing acetic acid and propionic acid and fermentation residues; mixing the fermentation residues with waste sugar residues, and performing pyrolysis to prepare biochar; and synchronously adding the liquid carbon source and the biochar into a denitrification reactor, and carrying out denitrification on the landfill leachate. The total nitrogen removal load of the landfill leachate denitrification system is increased by 60% compared with that of a single carbon source process, and the sugar manufacturing solid waste recycling rate gt is achieved; 95%. According to the method disclosed by the invention, the dual goals of efficient denitrification and solid waste recycling are achieved through cooperative treatment of the waste molasses and the waste sugar residues in the sugar manufacturing process.

The invention discloses a fermentation medium for regulating and controlling a clostridium aerovorans target product and a fermentation method of the clostridium aerovorans target product. The medium comprises a basic component and a regulating component, the basic components comprise ammonium chloride, potassium chloride, magnesium sulfate heptahydrate, sodium chloride, monopotassium phosphate, yeast powder, calcium chloride dihydrate, sodium selenite, nickel chloride hexahydrate, biotin, folic acid, pyridoxine hydrochloride, thiamine, riboflavin, nicotinic acid, D-calcium pantothenate, cobalamin, para aminobenzoic acid and lipoic acid; the adjusting component comprises sodium tungstate, ferrous sulfate and sodium molybdate, sodium tungstate and ferrous sulfate have a forward adjusting effect on increasing the yield of acetic acid, ethanol and butanol in the target product, and sodium molybdate has a reverse adjusting effect on the target product. By optimizing the proportion of trace elements in the culture medium, the clostridium metabolism efficiency is improved, synthesis of a target product is regulated and controlled, and the method is particularly suitable for industrial production of acetic acid, ethanol, butanol and the like.

The invention discloses a clostridium corpse strain and application thereof. The preservation number of the clostridium cadaveris J038 strain of the clostridium cadaveris J038 strain is CGMCC (China General Microbiological Culture Collection Center) NO. 35440, and the clostridium cadaveris J038 strain of the clostridium The clostridium corpse J038 strain is separated from pig manure for the first time, and 4-hydroxyphenylpropionic acid is produced by metabolism of the clostridium corpse J038 strain. In addition, the invention further provides application of the clostridium corpse in preparation of 4-hydroxyphenylpropionic acid, and when the clostridium corpse is adopted to prepare 4-hydroxyphenylpropionic acid, the clostridium corpse has the advantages of being small in environmental pollution, high in sustainability, precise, green, efficient and the like; the yield is high, and the increasing requirements of the fields of medicine, food, agriculture and the like on the 4-hydroxyphenylpropionic acid can be met.

The invention provides a microecological preparation with an immunological enhancement function and application of the microecological preparation in antibiotic-free breeding of livestock and poultry, and relates to the technical field of agricultural biology. The microecological preparation with the immunological enhancement function comprises a probiotic component, a prebiotics component and an immunological enhancement component, and the probiotic component, the prebiotics component and the immunological enhancement component are respectively composed of lactic acid bacteria, bacillus, clostridium butyricum, fructo-oligosaccharide, yeast beta-glucan, astragalus polysaccharide and chitosan oligosaccharide. Wherein the lactic acid bacteria accounts for 30%-40%, the bacillus accounts for 20%-30%, the clostridium butyricum accounts for 10%-20%, the fructo-oligosaccharide accounts for 5%-10%, the yeast beta-glucan accounts for 3%-8%, the astragalus polysaccharide accounts for 2%-5%, and the chitosan The probiotics can enhance immunity and regulate intestinal tracts at the same time, the effect is improved by 40% or above compared with common probiotics, animals suffer less illness and grow fast, flora can resist high temperature and gastric acid through a special protection technology, the loss of viable bacteria is less than 5% after the probiotics are stored for 3 months, and the probiotics are stable by two times compared with conventional products.

The invention discloses an application of some functional genes in improving the performance of clostridium for synthesizing ethanol by utilizing one-carbon gas fermentation, wherein overexpression genes NCBI (National Center for Biotechnology Information) are CLJUc04490, CLJUc25060, CLJUc06990, CLJUc22650 and / or CLJUc07720, and expression of inhibition genes CLJUc09160 and / or CLJUc23840 can be used for improving the autotrophic growth capability and the ethanol synthesis yield of clostridium Yangdall when the clostridium Yangdall is fermented by utilizing C1 gas; therefore, the economical efficiency of the clostridium yangdall fermentation is improved.

The present invention belongs to the field of biotechnology, and discloses a composition for reducing the emission of odorous substances from poultry, comprising rutin or mulberry leaf extract, Clostridium butyricum, and Bacillus subtilis; the mass ratio of rutin or mulberry leaf extract, Clostridium butyricum, and Bacillus subtilis is 1-3:0.5-1.5:1; the specification of Clostridium butyricum is 1 billion CFU / g, and the specification of Bacillus subtilis is 1 billion CFU / g. The composition utilizes the flavonoid additive rutin or mulberry leaf extract, Bacillus subtilis, and Clostridium butyricum in combination, which can significantly improve the ability of Bacillus subtilis and Clostridium butyricum to inhibit odor. At the same time, the present invention also discloses the application and feed of the composition.

The invention discloses clostridium butyricum as well as a culture method and application thereof, belongs to the field of feed additives, and aims to solve the problems that a controllable fattening period is shortened and the efficacy of the clostridium butyricum cannot be completely exerted due to the fact that a wide range of clostridium butyricum needs a relatively long adaptation period for an endogenous intestinal environment of an animal. The clostridium butyricum from the North Naa Biotechnology Co., Ltd. Is cultured by simulating an animal endogenous environment in vitro, and after the clostridium butyricum which completely adapts to animal endogenous is obtained, the screened animal endogenous clostridium butyricum is coated by adopting a coating technology, so that the clostridium butyricum is not damaged by a strong acid environment in an animal stomach bag; the clostridium butyricum strain has the advantages that the clostridium butyricum strain can be used for preparing animal endogenous clostridium butyricum, a large quantity of animal endogenous clostridium butyricum can reach intestinal tracts and be released, and the animal endogenous clostridium butyricum can quickly adapt to animal intestinal environment due to higher adaptability of the strains, and can quickly form efficient colonization and functional expression under the condition of almost no adaptation period. The feed additive can be used as a biological feed additive.

The invention belongs to the field of microorganisms. The invention provides an application of a probiotic composition in preparation of medicines for preventing and treating ulcerative colitis, the probiotic composition comprises a combination of bacillus coagulans and clostridium butyricum, and the combination of bacillus coagulans and clostridium butyricum comprises a combination of bacillus coagulans and clostridium butyricum. The bacillus coagulans is bacillus coagulans JSSW-LA-07-1 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.10182, and the clostridium butyricum is clostridium butyricum JSIM-MCB20040312 with the preservation number of CGMCC No.1647. The bacillus coagulans and the clostridium butyricum are used for preparing the bacillus coagulans and the clostridium butyricum. The probiotic composition has the capacity of preventing and treating ulcerative colitis, the ulcerative colitis treating effect is better than that of positive control medicine salazosulfapyridine, the obvious ulcerative colitis treating effect is achieved, the inflammatory factor content and the oxidative stress level are remarkably reduced, inflammatory cell infiltration is reduced, intestinal flora imbalance is improved, and the effect of preventing and treating ulcerative colitis is achieved. Meanwhile, the effect of promoting planting of probiotics is achieved.

The invention discloses a method for promoting acid production and alcohol production through fermentation of clostridium perdali C1 gas by using a biological source carbon-based redox regulation and control medium. The preparation method comprises the following steps: obtaining biochar by adopting a high-temperature pyrolysis method, grinding and sieving to obtain a biogenic carbon-based redox regulation medium, adding the biogenic carbon-based redox regulation medium into a fermentation culture medium, inoculating clostridium perdali into a fermentation device filled with the fermentation culture medium, controlling the volume ratio of H2 to CO2 in the fermentation device to be (1.5: 1)-(2.5: 1), and carrying out anaerobic fermentation for 20-30 days to obtain the biogenic carbon-based redox regulation medium. According to the invention, a biological source carbon-based redox regulation medium is introduced into a clostridium perdali gas fermentation system, and the medium not only can enrich CO2 gas, but also can improve the mass transfer efficiency of a gaseous substrate; the method has the advantages that the method is simple and convenient to operate, extra reducing power is provided for a fermentation system, electron transfer is mediated, the CO2 utilization rate of the strain and the fermentation product generation rate are remarkably increased, secondary resource utilization of solid waste biomass is achieved, C1 gas is effectively converted into high-value chemicals, and a green, economical and sustainable development road is provided for conversion of CO2.

The invention relates to the technical field of microorganisms, and provides Witzia ciliaris KY009 (preservation number is CGMCC (China General Microbiological Culture Collection Center) NO. 33480) and Clostridium butyricum KY002 (preservation number is CGMCC NO. 33478), and the two strains are separated from an excrement sample. Experiments prove that the two strains can obviously improve the sperm quality and quantity, regulate the sex hormone level, improve the intestinal inflammation symptom and regulate the expression of related inflammatory factors. The invention also provides a composition containing the two strains, wherein the composition comprises a food composition, a pharmaceutical composition, a health-care product composition, a functional microbial agent and the like. The compositions are useful for improving reproductive health, modulating sex hormone levels, treating intestinal inflammation and promoting mucosal repair, and alleviating metabolic and immune related diseases. The strain and the composition provided by the invention have wide application prospects in the fields of medicines, foods and health-care products.

The invention relates to a clostridium difficile triple detection kit based on colloidal gold immunochromatography and a preparation method of the clostridium difficile triple detection kit, and belongs to the technical field of immunodetection. The clostridium difficile triple detection kit provided by the invention comprises a clostridium difficile triple detection card and a sample diluent, and the clostridium difficile triple detection card is formed by arranging and connecting three detection test strips in parallel; the detection test strip comprises a clostridium difficile toxin A detection test strip, a clostridium difficile toxin B detection test strip and a clostridium difficile glutamate dehydrogenase detection test strip. By using the clostridium difficile triple detection kit provided by the invention, three markers related to clostridium difficile can be detected at the same time, the detection limit of clostridium difficile toxin A is 0.25 ng / mL, the detection limit of clostridium difficile toxin B is 0.5 ng / mL, and the detection limit of clostridium difficile glutamate dehydrogenase is 0.5 ng / mL, so that the clostridium difficile triple detection kit has the advantages of good sensitivity, simplicity in operation, high sensitivity, high sensitivity, high sensitivity and the like. The method is suitable for large-scale popularization screening.

The invention discloses bacillus amyloliquefaciens with the characteristic of inhibiting mould growth and application of the bacillus amyloliquefaciens, and belongs to the field of microorganisms and fermentation engineering, the bacillus amyloliquefaciens is preserved in China General Microbiological Culture Collection Center on August 5, 2024, and the preservation number is CGMCC No.31538. The strain is bacillus amyloliquefaciens obtained by screening, separating and purifying from peasant pickles, and the strain can inhibit the growth of escherichia coli, clostridium perfringens and mould. The bacillus amyloliquefaciens disclosed by the invention solves the problem of insufficient effect of a microorganism mildew-proof method of the existing fermented feed, and the bacillus amyloliquefaciens is added into the biological fermented feed, so that the quantity of harmful microorganisms in the biological fermented feed can be effectively reduced, the generation of mycotoxin is inhibited, the feed is prevented from mildewing and going bad, and the shelf life of the feed is prolonged.

The invention relates to a clostridium perfringens phage and application thereof. The bacteriophage is a clostridium perfringens bacteriophage P2ACQJS03 (wherein the clostridium perfringens bacteriophage P2ACQJS03), and the preservation number of the clostridium perfringens bacteriophage P2ACQJS03 in the China Center for Type Culture Collection (CCTCC) is CCTCC NO: M 20242430. The bacteriophage provided by the invention is a virulent bacteriophage separated from the nature, tests prove that the bacteriophage has no toxic effect on normal microbial flora, and the DNA of the bacteriophage cannot encode virulence genes, so that the bacteriophage is high in stability; the bacteriophage disclosed by the invention is wide in host range, and large-scale industrial production can be realized. The bacteriophage provides an excellent strain resource for developing a novel antibacterial preparation, and has a good application and development prospect.

The invention discloses a method for improving utilization of synthesis gas of clostridium. The method comprises the following steps: blocking or down-regulating expression of a transcription factor NrdR; and / or enhancing the expression of a transcription factor DeoR. The method provided by the invention effectively improves the utilization and conversion efficiency of the clostridium aerovorans on the carbon gas such as synthesis gas, and has popularization and application values.

The invention discloses a method for preparing a Clostridium perfringens epsilon toxin-ferritin nanoparticle antigen, comprising constructing a soluble E toxin mutant and a soluble Ferritin protein respectively and purifying them, and then self-assembling the soluble E toxin mutant and the soluble Ferritin protein to form a nanoparticle antigen. The epsilon-ferritin nanoparticles prepared by the invention significantly promote the production of IgG in antigen-immunized mice and successfully induce a cellular immune response.

The invention discloses a method and a system for producing high-purity medium-chain fatty acid (MCFA) based on waste molasses two-stage regulation fermentation. Comprising the following steps: (1) diluting waste molasses, then adding a compound enzyme agent of pectinase, beta-glucanase and cellulase, and coupling microwave to assist treatment; (2) inoculating the pretreated waste molasses with a composite flora containing a clostridium kluyveri mutant strain and a Megasphaera elsdeni engineering bacterium, and carrying out a fermentation treatment on the waste molasses, wherein the composite flora contains the clostridium kluyveri mutant strain and the Megasphaera elsdeni engineering bacterium; (3) sequentially performing two-stage dynamic regulation and control fermentation on the waste molasses inoculated with the composite flora through a pH-stat control strategy to realize efficient conversion from butyric acid to hexanoic acid / octanoic acid; and (4) a hydrophobic hollow fiber membrane module is adopted to realize on-line removal of the product, and the inhibition effect of the product is relieved. According to the technology, the yield of the MCFA produced from the waste molasses reaches 0.45 g / g COD, the cost is reduced by 68% compared with that of a traditional substrate, and a new way is provided for recycling of sugar manufacturing waste.

The invention provides a microbial agent for producing acid through fermentation of organic waste and application of the microbial agent, and relates to the technical field of biology, the microbial agent contains Acetobacter xylinum, Clostridium bovifaecis and Eubacter mucilaginosus, and the microbial agent is prepared from the following raw materials: Acetobacter xylinum, Clostridium bovifaecis and Eubacter mucilaginosus. Studies find that acetobacter burgii, clostridium cow dung and eubacterium mucilaginosus complement each other in the anaerobic fermentation process, the degradation efficiency of organic matters such as carbohydrates and proteins in the organic wastewater / perishable garbage is improved, the yield of volatile fatty acids is remarkably increased, meanwhile, the emission of CO2 is reduced, and the environmental pollution is reduced. The method has the application prospects of organic wastewater / perishable garbage treatment and recycling. When the acid-producing bacterium agent is applied to organic wastewater / perishable garbage, the fermented mixed acid liquid is rich in VFA and can be used as an organic carbon source, and the additional value is higher; when the acid-producing bacterium agent is applied to organic wastewater / perishable garbage, the operation is simple and convenient, the cost is low, biogas slurry treatment links and cost are reduced, the added value of products can be improved, and the acid-producing bacterium agent has an engineering application prospect.

The present invention belongs to the field of biotechnology, and specifically relates to the use of the CD630_27900 gene in reducing the tolerance of Clostridium difficile. Knocking out the CD630_27900 gene can reduce the tolerance of Clostridium difficile to acidic environments and antibiotics and reduce its cytotoxicity; the sequence of the CD630_27900 gene is shown in SEQ ID NO.1; the antibiotics include ampicillin, metronidazole, amoxicillin, vancomycin, norfloxacin, cefoxitin, clindamycin and kanamycin. The research results of the present invention suggest that the CD630_27900 gene can be used as a target for inhibiting the drug resistance and reducing the toxicity of Clostridium difficile, providing a new idea for the treatment of Clostridium difficile infection (CDI).

The invention relates to an ink-jet printing ink formula and a preparation method thereof, and belongs to the technical field of ink preparation. The carbon black color paste specifically comprises the following raw materials in parts by mass: 3 to 7 parts of carboxylated carbon black, 2 to 4 parts of modified protein, 1 to 3 parts of a dispersant, 0.1 to 0.3 part of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, 0.1 to 0.12 part of N-hydroxysuccinimide, 1 to 5 parts of a 2-morpholinoethanesulfonic acid buffer solution, 0.1 to 0.3 part of an auxiliary agent and 10 to 50 parts of a composite solvent. The polyethylene glycol modified ethanol clostridium protein is used in the components, and carboxylated carbon black and the dispersing agent are combined, so that the compatibility among the components in the system is improved, and good thermal stability and system stability are achieved while the particle size of the black ink is refined.

The invention provides a probiotic mixed fermentation method for enhancing a synergistic effect. Lactobacillus plantarum, bifidobacterium longum and clostridium tyrobutyricum are inoculated according to a volume ratio, and a pH-triggered two-stage fermentation process is adopted: the first stage is micro-aerobic to drive lactic acid bacteria to quickly produce acid, and the second stage is anaerobic to directionally improve butyric acid synthesis by pulse feeding of inulin-cysteine sustained-release particles. Physical isolation of flora is realized by using a double-layer gradient carrier, lactic acid bacteria are immobilized by sodium alginate-gelatin gel on the outer layer to form an acid barrier, and bifidobacterium and butyric acid producing bacteria are co-immobilized by chitosan microspheres on the inner layer to construct an anaerobic microenvironment. The technology breaks through the competitive inhibition bottleneck of multi-strain fermentation, the total number of viable bacteria is increased by 150% compared with a traditional technology, the yield of butyric acid is increased by 355%, and the technology is suitable for large-scale production of functional food and pharmaceutical preparations.

The invention discloses an in-situ remediation method for uranium pollution and application of the in-situ remediation method. According to the in-situ remediation method for uranium pollution, pyrite is driven to be hydrolyzed, oxidized, subjected to atomic rearrangement and biomineralized through interaction of microorganisms and pyrite, uranium pollutants are fixedly sealed and stored in reconstructed product iron oxide lattices, uranium pollution areas are remedied, and the microorganisms comprise one or more of brucella, geobacter and clostridium. If the uranium polluted area contains microorganisms, natural humus is used as an electron donor to stimulate in-situ microorganisms. The natural humus solution is injected into the uranium polluted area in a circulating pumping and injecting mode, in-situ microorganisms are stimulated, the concentration of the natural humus solution is 0.05-5 g / L, and the dosage of the natural humus solution is 5-500 g / day. According to the method, in-situ minerals, microorganisms and geochemical resources are fully considered and reasonably utilized, the engineering cost is saved by means of the in-situ occurrence interaction relation of the minerals and the microorganisms, and the remediation efficiency is high.

The invention provides application of an Alzheimer's disease intestinal flora biomarker combination in preparation of a product for detecting AD (Alzheimer's disease). The marker combination comprises ackermania muciniphila, helicobacter hispiduli, dunaliella, pantoea, other fungus, lactobacillus, clostridium erysipelas, helicobacter, enterobacter, laryngobacter, clostridium and undefined genus of clostridium erysipelas, and is characterized in that the marker combination comprises ackermania muciniphila, helicobacter hispiduli, dunaliella, pantoea, other fungus, lactobacillus, enterobacter, laryngobacter, clostridium and undefined genus of clostridium erysipelas. And the composition is any one or a combination of at least two of bacillus pumilus and odorobacter. According to the present invention, the abundance level of the intestinal flora biomarker combination is adopted as the detection index, and the kit is used for the early auxiliary diagnosis of the Alzheimer's disease, has characteristics of high detection precision, convenience, rapidness, safety and non-invasive property, and provides important clinical guiding significance for the auxiliary diagnosis of AD-related indexes.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a method for reducing fiber-binding protein content by microbial fermentation of sesame meal, in order to effectively utilize nutritional ingredients of the sesame meal, firstly, aspergillus oryzae yeast and kluyveromyces marxianus are utilized for aerobic fermentation, then enzymolysis is performed, and finally, the content of the fiber-binding protein is reduced. Finally, lactobacillus plantarum and clostridium butyricum are inoculated for anaerobic fermentation, the obtained sesame seed meal fermentation product contains various high-content nutritional ingredients including lactic acid, small peptide and crude protein, the content of fiber binding protein in neutral detergent fibers is reduced, the sesame seed meal fermentation product is used for animal feed, the feed-weight ratio can be effectively reduced, and the feed utilization rate is increased. The diarrhea rate of the piglets is reduced, and the growth performance of the piglets is improved.

The invention discloses a method for constructing an engineered bacterium of Clostridium aeruginosa, comprising the following steps: preparing a desired gene in a biosynthetic pathway of a target product, namely a pathway gene, to form a pathway gene cluster; embedding the pathway gene cluster into a transposase recognition site of a mariner transposon to form a pathway gene expression cassette; selecting a constitutive strong promoter commonly used in Clostridium jundal for driving transposase expression, loading the pathway gene expression cassette into a multiple cloning site of a plasmid, and inserting an inducible repressor element upstream of the replicon to obtain a transposition plasmid; transforming the transposition plasmid into Clostridium aeruginosa to obtain an engineered bacterium of Clostridium aeruginosa. The yield of 3-hydroxybutyric acid expressed by the engineered bacterium constructed by the above method is as high as 3g / L.

The invention belongs to the field of aquaculture feed additives, and particularly relates to a compound preparation with a synergistic effect of an exocarpium citri grandis extract and probiotics and a preparation method of the compound preparation, and the compound preparation is used for preventing and treating enterohepatic cytozoonosis of penaeus vannamei. Comprising an exocarpium citri grandis extract preparation step, a bacillus subtilis and clostridium butyricum culture step and a compound exocarpium citri grandis feed additive preparation step. 0.1%-0.5% of the compound exocarpium citri rubrum feed additive is added into shrimp feed, and penaeus vannamei is fed; compared with a direct feeding feed, the compound exocarpium citri rubrum feed additive has the advantages that the morbidity of the enterocytozoonosis hepatopenaei of the shrimps is effectively reduced, and meanwhile, the occurrence of other diseases is reduced.

The invention relates to the technical field of probiotic preparations, in particular to a process for preparing intestinal conditioning prebiotics from clostridium butyricum and radix astragali seu hedysari, which comprises the following steps: (1) multi-stage synergistic activation of clostridium butyricum; (2) carrying out composite enzymolysis on the astragalus extract; (3) symbiotic fermentation; (4) performing multi-layer embedding; the prepared prebiotics have a multi-dimensional intestine regulating mechanism, the bacteriostasis and mucous membrane repairing functions of the clostridium butyricum cooperate with the prebiotics effect and antioxidant activity of the astragalus polysaccharide to form a multi-protection system, and the intestinal flora imbalance is more effectively improved compared with single probiotics or prebiotics. The multi-layer embedding technology solves the problems of gastric acid inactivation and difficulty in intestinal colonization of probiotics, and spore induction of three-stage fermentation is matched, so that the viable count maintenance rate of the product is kept at a relatively high level after the product is stored for 6 months at normal temperature, and the shelf life is remarkably prolonged.

The invention discloses a culture medium for A type clostridium perfringens and C type clostridium perfringens, a fermentation culture method and application of fermentation toxin production. Wherein the culture medium comprises the following components: tryptone, # imgabs0 # peptone, casein peptone, a yeast extract, lactoalbumin hydrolysate, trihydroxymethyl aminomethane, sodium acetate and glucose. According to the present invention, through the specific formula composition, the A-type clostridium perfringens and the C-type clostridium perfringens can adapt to fermentation in the fermentation tank, and in the fermentation culture process, the toxin production ability of the A-type clostridium perfringens and the C-type clostridium perfringens can be significantly improved by controlling the stirring speed, the pH value of the fermentation liquid and the precise material supplementing manner. And the supernate obtained by fermentation is used as a raw material for preparing the vaccine, so that animals can generate high-level neutralizing antibodies aiming at the clostridium perfringens A and C type toxins after being immunized with the vaccine, the death of the animals caused by infection of the clostridium perfringens A and C type toxins is avoided, and the method has practical popularization and application values.