181 results about "Clostridiales" patented technology

The invention discloses saccharomyces boulardii, a culture and a preparation method and application of the saccharomyces boulardii. The preservation number of the strain is CCTCC (China Center for Type Culture Collection) NO: M 20252633. The preparation method comprises the following steps: preparing saccharified liquid by taking white spirit vinasse or brewer's grains as a main raw material and adopting a high-temperature alkali treatment and composite enzymolysis process, inoculating strains for liquid-state high-density fermentation, carrying out solid-liquid separation on fermentation liquor, taking a liquid phase, and carrying out spray drying. According to the method, high-value utilization of the waste vinasse is realized, and the production cost is remarkably reduced. The obtained culture is rich in viable bacteria and active small peptides, and has the characteristics of strong tolerance, wide antibacterial spectrum and the like. As a feed additive, the feed additive can effectively reduce the diarrhea rate of piglets, inhibit clostridium perfringens and prawn vibrios of broilers, and improve the oxidation resistance and production performance of animals.

Provided is a growth inhibitor for Clostridioides difficile. A growth inhibitor for Clostridioides difficile, comprising killed Enterococcus faecalis. Also provided are a parenteral pharmaceutical preparation, a bactericide, a disinfectant, an antibacterial agent, a sanitizer or a detergent for inhibiting the growth of Clostridioides difficile.

The application belongs to the technical field of biology and relates to a Clostridium butyricum engineering strain for improving butyric acid synthesis through a coenzyme A transferase pathway as well as a construction method and application of the Clostridium butyricum engineering strain. Clostridium tyrobutyricum The application expresses a butyryl coenzyme A:acetic acid-coenzyme A transferase gene from Clostridium tyrobutyricum Ctcat1 A coenzyme A transferase (CoAT) pathway is constructed, the pathway synthesizes butyric acid by taking acetic acid as a substrate, therefore, the content of acetic acid in a fermentation product is greatly reduced, and the butyric acid yield is significantly improved.

Compounds to treat various infections, including infections caused by Clostridioides difficile, particularly the compounds α-methylene and α-aminomethyl lactones, lactams, iminolactones, and iminolactams, thiolactones, thionolactones, thiolactams, and thionolactams; pharmaceutical compositions; and methods for treating those infections.

The invention discloses a micropterus salmoides intestinal function feed additive based on composite synergy and application thereof, and belongs to the technical field of feed additives. The feed additive for improving the intestinal function of the largemouth bass comprises the following raw materials: clostridium butyricum, tributyrin, aspergillus niger and a plant source carrier. The invention provides a compound feed additive capable of remarkably improving the growth performance of the largemouth bass, improving the digestive enzyme activity of the intestinal tract and optimizing the intestinal barrier, and the compound feed additive can remarkably improve the weight gain rate and the specific growth rate of the largemouth bass, remarkably improve the digestive enzyme activity of the intestinal tract of the largemouth bass and maintain the integrity of the tissue structure of the intestinal tract. Compared with a single additive, the compound additive shows a better synergistic gain effect in the aspect of comprehensive effects, has important value for promoting research, development and practical application of novel feed additives of micropterus salmoides, has good popularization and application prospects, and is expected to bring remarkable economic benefits to the breeding industry.

The invention provides a clostridium thermocellum engineering strain with a high nitrogen utilization rate. NitR regulatory protein is precisely overexpressed in the engineering strain; the precise overexpression is as follows: the expression level of the regulatory protein NitR is 1.2-2.0 times that of a wild type. A chassis strain of the strain is clostridium thermocellum GB2 with a glucosidase expression cassette on a genome, and the clostridium thermocellum GB2 is an engineering strain for expressing BGL in clostridium thermocellum DSM1313. The inventor accidentally finds that when the expression level of the regulatory protein NitR is controlled to be 1.2-2.0 times that of a wild type, the growth ability and biomass accumulation of the engineering strain under the nitrogen-deficient condition are remarkably improved, so that the utilization efficiency of a nitrogen source is increased, and an unexpected technical effect is achieved. The invention further provides a construction method of the clostridium thermocellum engineering strain and application of the clostridium thermocellum engineering strain in saccharification of lignocellulose raw materials. The engineering strain is particularly suitable for saccharification treatment of low-nitrogen-content lignocellulose raw materials (such as corn straw and corn cob residues), the saccharification efficiency is improved while the nitrogen source adding cost is remarkably reduced, and the engineering strain has important significance on industrial application.

The application discloses a three-stage linkage hydrogen production method based on kitchen waste, and relates to the technical field of biological enzymes, and comprises the following steps: step I, pretreatment; step II, dark fermentation: feeding the material treated in step I into a dark fermentation tank, inoculating Clostridium beijerinckii with the preservation number of ATCC NO.35702; step III, photo fermentation: feeding the first fermentation liquor into a photo fermentation tank, inoculating Rhodopseudomonas palustris with the preservation number of CGMCC 1.2180, adding 50 mg / L-100 mg / L MoFe nanoparticles, and collecting the gas produced in the fermentation. The three-stage linkage of the synergistic pretreatment, dark fermentation and photo fermentation realizes the efficient and stable conversion of the kitchen waste into hydrogen.

This invention provides a strain derived from Clostridium difficile (Clostridium difficile) Clostridioides difficile This invention relates to mutants of glutamate dehydrogenase and their preparation, including single-site, two-site, or multi-site mutants. The invention effectively solves the problems of inactivity or low activity of glutamate dehydrogenase in the biocatalytic transformation of 2-carbonyl-4-(hydroxymethylphosphono)butyric acid as a substrate, and provides a continuous chemical method for the synthesis of 2-carbonyl-4-(hydroxymethylphosphono)butyric acid using a microchannel reactor throughout the entire process.

The invention discloses a clostridium butyricum preparation for preventing and treating porcine reproductive and respiratory syndrome and application of the clostridium butyricum preparation, and belongs to the technical field of microbial fermentation feed. According to the invention, the clostridium butyricum with the preservation number of CCTCC NO: M 20231636 is subjected to activation culture, seed culture and high-density fermentation culture to prepare the clostridium butyricum preparation, and the effect of the clostridium butyricum preparation in prevention of PRRSV infection is developed. Experimental results show that when the clostridium butyricum preparation is added into a pig complete feed, the positive infection rate of the porcine reproductive and respiratory syndrome of a pig herd can be effectively reduced, the death number of the pig herd is remarkably reduced, and the clostridium butyricum preparation has outstanding prevention and treatment effects. The clostridium butyricum preparation provided by the invention is free from drug resistance and toxic and side effect risks, has the advantages of low cost, high safety, environment friendliness and the like, and has great significance in benefit improvement of the live pig breeding industry.

The invention belongs to the technical field of biological medicines, and particularly relates to a composition for improving a cognitive function as well as a preparation method, a product and application of the composition. The composition is prepared by taking soybean meal, whey protein peptide powder and bovine bone collagen peptide powder in a mass ratio of 1: 3: 2 as peptide source raw materials, matching the peptide source raw materials with AMY001, lactobacillus plantarum LP-90 and clostridium butyricum (3: 1: 1) compound strains, and carrying out enzyme production self-hydrolysis by bacillus amyloliquefaciens, peptide-bacterium anaerobic synergistic fermentation and sodium alginate-HPMC-CPP composite carrier embedding. According to the present invention, the hippocampus Abeta1-42 protein can be cleared, the BDNF content can be increased, the IL-6 expression inhibition multi-mechanism synergistic effect can be achieved, the memory improvement rate and the Abeta1-42 clearance rate are high, and the cognitive impairment can be significantly repaired. The composition is high in stability and bioavailability, controllable in preparation process and suitable for development of food and health care products for improving the cognitive function and medicines for preventing or assisting in improving diseases related to cognitive impairment.

The invention relates to the technical field of biological detection, in particular to an LAMP (loop-mediated isothermal amplification) detection kit for clostridium difficile tcdA and tcdB. The kit comprises a specific LAMP primer group, an LAMP amplification reaction mixed solution, a lysis solution, a positive reference substance, a negative reference substance and an operation instruction. The system is based on a loop-mediated isothermal amplification (LAMP) principle, nucleic acid detection of toxin-producing genes tcdA and tcdB of clostridium difficile is realized under the condition of 61-65 DEG C, and a detection result is judged through colorimetric change of HNB or a fluorescence signal; according to the system, an amplification reaction can be completed without a thermal cycler, the reaction time is about 50 minutes, and rapid detection can be realized on site; the method disclosed by the invention has the advantages of high sensitivity, strong specificity, simplicity and convenience in operation and low equipment dependence, and is suitable for detecting the toxin-producing gene of the clostridium difficile in medical institutions, primary laboratories and public health monitoring.

The invention relates to the technical field of feed additives, and particularly discloses a feed mildew removing agent and a preparation method thereof. The feed mildew removing agent provided by the invention is prepared from the following raw material components: bacillus microcapsules, clostridium butyricum microcapsules, modified montmorillonite, saccharomyces cerevisiae cell walls and a vinasse fermentation synergist. Through the design of coating micro-capsules of bacillus and clostridium butyricum, the survival rate of flora in the transportation and storage stages is improved, and effective mold removal is promoted by cooperating with modified montmorillonite toxin adsorption; furthermore, the vinasse fermentation synergist prepared on the basis of bacillus subtilis-lactobacillus two-step fermentation is endowed with the functions of integrating nutrition supply, toxin inhibition and flora regulation and control, and various substances generated by fermentation not only directly improve nutrition for animals, but also help flora to improve the intestinal toxin removal efficiency; and clostridium butyricum is matched to reduce the local pH of the intestinal tract, inhibit the proliferation of toxin-producing mould and reduce the intestinal tract colonization of the mould.

The invention relates to the technical field of biological medicine, in particular to a composition for blocking formation of phage treatment tolerance and application of the composition. The composition comprises a folium ilicis purpurea ethyl acetate extraction active component and a clostridium perfringens specific bacteriophage, the folium ilicis purpurea ethyl acetate extraction active component is prepared from the following components in percentage by mass: 8 to 15 percent of protocatechuic acid and 5 to 10 percent of ethyl gallate, and the mass ratio of the protocatechuic acid to the ethyl gallate is (1.5 to 1) to (2.5 to 1); the clostridium perfringens specific bacteriophage is a virulent bacteriophage capable of specifically cracking a clostridium perfringens NetB toxin positive strain. According to the invention, the shallow thought that the existing compounding technology only pursues synergistic sterilization is subverted, and a deep synergistic mode of bacteriophage splitting attack and folium ilicis purpurea component disintegration bacterial defense program is proposed and verified for the first time. And the formation of tolerance is fundamentally blocked, so that a more thorough and more lasting treatment effect is realized, and the comprehensive production performance of animals is accidentally and greatly improved on the basis of the treatment effect.

Antibody-based binding agents derived from human and camelid immunoglobulins are described, as well as strains of yeast engineered to secrete the binding agents, and methods of treating and preventing Clostridium difficile infections using the engineered strains of yeast. These binding agents recognize and bind with specificity to Clostridium difficile toxin A and / or toxin B and in some cases exhibit toxin neutralizing activity. The binding agents include camelid VHH peptide monomers, linked groups of VHH peptide monomers, VHH peptide monomers joined to antibody Fc domains, and VHH peptide monomers joined to IgG antibodies.

This invention provides a method for producing Clostridium neurotoxins containing an exogenous activation loop, modified Clostridium neurotoxins, and pharmaceutical compositions. [Solution] A method for proteolytically processing a single-chain Clostridium neurotoxin into a corresponding double-chain Clostridium neurotoxin, comprising: providing a single-chain Clostridium neurotoxin; and contacting the single-chain Clostridium neurotoxin with enterokinase or factor Xa, wherein the single-chain Clostridium neurotoxin has the polypeptide sequence Cys-(Xaa) a -Ile-Asp / Glu-Gly-Arg-(Yaa) b - A method in which an activation loop containing Cys (SEQ ID NO: 1) is present, a=1 to 10, b=4 to 15, and enterokinase or factor Xa hydrolyzes the peptide bond of the activation loop, thereby producing a double-stranded Clostridium neurotoxin.

The invention belongs to the technical field of microbial preparations, and particularly relates to a compound fermentation composition for regulating animal immunity and a preparation method and application thereof. The composition is prepared from the following components in parts by weight: 1 to 10 parts of lactobacillus plantarum polysaccharide, 1 to 5 parts of clostridium butyricum, 0.5 to 2 parts of bifidobacterium, 0.5 to 5 parts of beta-glucan, 50 to 70 parts of corn starch and 0.1 to 1 part of silicon dioxide. The lactobacillus plantarum polysaccharide prepared by a specific preparation process, the clostridium butyricum, the bifidobacterium, the beta-glucan, the corn starch and the silicon dioxide are scientifically matched in a specific amount ratio range, so that the synergistic interaction effect of the immunoregulation component, the intestinal micro-ecological regulation component and other functional components is realized; according to the present invention, the IgA content in animal serum can be significantly increased so as to achieve high efficiency and long-term immunoregulation, the maximization of the utilization of each component is achieved, and the safe and effective bioactive substance is finally provided for the improvement of animal immunity.

The invention provides application of a composition containing butyric acid producing bacteria and vitamin K2 in preparation of a medicine for treating osteoporosis, and relates to the technical field of biological medicines, the composition comprises butyric acid producing bacteria and vitamin K2; wherein the butyric acid producing bacteria are selected from any one or more of clostridium butyricum, rosella and clostridium praeparatum; and the vitamin K2 is selected from any one or more of menadione-4 or menadione-7. The composition can play a synergistic effect through an intestine-bone axis, and compared with a single component, the composition can more remarkably improve the bone mineral density. The composition has comprehensive effects and high safety, provides a brand-new nutrition intervention strategy with a synergistic effect for prevention and treatment of osteoporosis, and provides a brand-new possibility for preparation of medicines related to osteoporosis.

The invention discloses a recombinant clostridium praeparatum fpPRPS protein, the amino acid sequence of the recombinant protein is shown as SED ID No.3, and the nucleotide sequence of a gene for coding the recombinant protein is shown as SED ID No.2. The invention further discloses a preparation method of the recombinant clostridium praeparatum fpPRPS protein. Experiments prove that the recombinant clostridium praeparatum fpPRPS protein prepared by the invention has effective anti-inflammatory and anti-tumor components, and is simple to prepare, good in activity, high in purity, low in toxicity and easy to popularize and apply clinically and commercially; the invention has important significance for effectively treating and relieving intestinal inflammation and occurrence of various tumors including intestinal tumors and treating various Th17-related inflammations or tumors.

The invention relates to the field of bacteriostasis of clostridium difficile, in particular to bacillus coagulans, clostridium butyricum, a combination of the bacillus coagulans and the clostridium butyricum and application of the bacillus coagulans and the clostridium butyricum in relieving clostridium difficile infection. The bacillus coagulans is bacillus coagulans BC99 of which the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO. 21801, and the bacillus coagulans BC99 is a bacillus coagulans BC99 of which the preservation number is CGMCC NO. The clostridium butyricum is a clostridium butyricum CB39 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO. 33734, and the clostridium butyricum is a clostridium butyricum CB39 with the preservation number of CGMCC NO. The invention discloses inhibition effects of the bacillus coagulans, the clostridium butyricum and the combination thereof on clostridium difficile, and regulation and improvement of intestinal infection caused by the clostridium difficile.

The invention belongs to the technical field of gene engineering, and particularly relates to recombinant lactobacillus for expressing clostridium perfringens multiple antigens of targeted dendritic cells as well as a construction method and application of the recombinant lactobacillus. The recombinant lactobacillus contains an expression vector for expressing a fusion gene; the fusion gene comprises sequences for coding and expressing a PgsA '-fimA fusion protein, a LysM-Fba fusion protein, an LP1261-NetB fusion protein, a phi54 targeting element and a Pas escape element; a sequence for coding the PgsA '-fimA fusion protein is shown as SEQ ID NO: 1, a sequence for coding the LysM-Fba fusion protein is shown as SEQ ID NO: 2, and a sequence for coding the LP1261-NetB fusion protein is shown as SEQ ID NO: 3. When the recombinant lactobacillus is used for immunizing animals, mucosal immunity, cellular immunity and humoral immunity reactions can be induced, the immunocompetence of organisms can be comprehensively enhanced, and the recombinant lactobacillus can be used for preparing oral vaccines for prevention and control of chicken necrotic enteritis.

The application discloses a method for improving hydrogen production performance of Clostridium butyricum by using hematite nanoparticles, and belongs to the technical field of biological hydrogen production and microbial metabolism regulation. The method improves the hydrogen production performance by adding hematite nanoparticles into an anaerobic fermentation hydrogen production system of Clostridium butyricum for anaerobic fermentation. The application first discloses a specific double regulation mechanism of hematite nanoparticles on hydrogen production of Clostridium butyricum. By using hematite nanoparticles as a core regulation material, and through precise concentration gradient addition and stage temperature synergistic regulation, the hydrogen production lag period can be greatly shortened, the initial hydrogen production rate can be improved, byproduct synthesis of butyric acid can be inhibited, and the efficient hydrogen production path of acetic acid can be strengthened. The core technical problems that existing iron-based additives cannot shorten the hydrogen production lag period, the initial hydrogen production efficiency is low, and product feedback inhibition is easily caused are solved. The method is simple to operate, has strong industrialization adaptability, and can be widely applied to industrialization batch and continuous production of dark fermentation biological hydrogen production.

The invention discloses a primer group for simultaneously detecting four equine digestive tract bacteria and application thereof, the primer group comprises primer sequences as shown in SEQ ID NO: 1-8, and the four equine digestive tract bacteria are salmonella enteritidis, salmonella typhimurium, clostridium difficile and lawsonia intracellular. Extracting total DNA (deoxyribonucleic acid) of a sample to be detected by using the excrement sample nucleic acid extraction kit; the total DNA is used as a template, and the primer group is used for multiple PCR reaction to obtain an amplification curve. According to the invention, a primer sequence with high sensitivity and specificity is adopted, so that the quality of a detection result is ensured; the detection method is simple to operate, time-saving and labor-saving; the detection flux is high, and the reagent consumable cost is low.

The invention discloses oral composite microspheres for treating ulcerative colitis based on intestinal ROS storm pathological axis regulation as well as a preparation method and application of the oral composite microspheres, and aims to solve the problems that an existing ulcerative colitis treatment means is difficult to reverse core pathological circulation, oral antioxidant drugs and nano-enzymes are easily inactivated by gastric acid, the targeting property is poor, and the treatment effect is poor. And when the probiotics are independently used, the oxidation resistance is insufficient. The oral composite microsphere is prepared by taking clostridium butyricum spores as a biological carrier, firstly loading Ir10-KB nano-enzyme with peroxidase, superoxide dismutase and catalase like activities, and then carrying out sodium alginate-calcium ion cross-linking embedding. Anti-gastric acid protection and colon targeted release are achieved through the pH response gel characteristic of the sodium alginate, the flora regulation and mucous membrane repair effects of clostridium butyricum spores and the efficient ROS removal, mitochondrial protection and PANoptosis inhibition functions of the Ir10-KB nano-enzyme are synergistically exerted, and the synergistic treatment purpose of precise delivery-efficient oxidation resistance-micro-ecological regulation is achieved.

The invention belongs to the technical field of microorganisms, and particularly discloses an application of Clostridium daunum in preparation of an anti-aging or anti-aging product. The clostridium ruber strain, the product thereof and the product containing the clostridium ruber strain have excellent antioxidant and anti-inflammatory effects, can effectively reduce the level of substances such as reactive oxygen induced by oxidative stress, activate an Nrf2 signal channel, remarkably up-regulate the expression of endogenous antioxidant substances, and improve the heat stress resistance and the body vitality, so that the aging of the body is delayed; the health state is improved.

The invention belongs to the technical field of chemical drugs, and particularly relates to application of a traditional Chinese medicine composition in preparation of drugs for treating recurrent clostridium difficile infection.The traditional Chinese medicine composition is prepared from 10-50 g of fresh ginger, 3-20 g of honey-fried licorice roots, 5-30 g of ginseng, 3-20 g of dried ginger, 3-30 g of scutellaria baicalensis, 3-30 g of pinellia ternate, 3-15 g of coptis chinensis and 10-30 g of Chinese dates. The traditional Chinese medicine composition is excellent in clinical effect, and in sample research on high-risk crowds such as old people and immunosuppression CDI relapse patients, the treatment scheme of the vancomycin combined with the ginger heart-fire purging decoction is remarkably superior to vancomycin single-drug treatment in the aspect of continuous clinical response rate at the main curative effect end point. This benefit is achieved mainly by reducing the recurrence rate of CDI by more than 60%. The combined scheme shows a robust curative effect in key subgroups such as the elderly and immunosuppressive subgroups, is good in safety, does not increase extra adverse event risks, and particularly does not observe renal function impairment.

In order to solve the technical problem of high-efficiency expression of CLMP protein in tumors in the prior art, the invention creatively provides a clostridium butyricum engineering strain. According to the engineering bacterial strain, ATCC19398 clostridium butyricum is used as a chassis bacterial strain, and the engineering bacterial strain comprises a recombinant expression vector for expressing CLMP protein. The inventor accidentally finds that the clostridium butyricum as a chassis strain can realize expression of CLMP in tumor cells, the technical problem of expression reduction of CLMP protein in colorectal cancer is fundamentally solved, and the clostridium butyricum has important significance in treatment of colorectal cancer. On the basis, the inventor knockout a purine synthesis related gene purM in the clostridium butyricum to realize the enrichment of the clostridium butyricum in tumors; further, by knocking out the protease gene lon, the expression quantity of CLMP protein is remarkably increased while precise positioning aiming at the tumor is met, so that the inhibition effect on the tumor is further improved. Moreover, the engineering strain can significantly inhibit the growth of tumors in a mouse model, is good in safety, provides a new idea for the treatment of colorectal cancer, and has important practical application value.

The invention provides a kit for detecting multiple piglet diarrhea pathogens and a detection method, and belongs to the technical field of biological information detection. The kit comprises a reaction reagent, wherein the reaction reagent comprises a porcine epidemic diarrhea virus specific primer and a probe, a porcine delta coronavirus specific primer and a probe, a piglet pathogenic escherichia coli specific primer and a probe, a clostridium perfringens specific primer and a probe, a buffer solution, an enzyme mixed solution and a freeze-drying protective additive. The detection method adopts the kit for detecting various piglet diarrhea pathogens. According to the kit disclosed by the invention, simultaneous detection of four target genes including the porcine epidemic diarrhea virus, the porcine delta coronavirus, the piglet pathogenic escherichia coli and the clostridium perfringens can be realized only through one-time amplification, so that the detection time is greatly shortened while the detection cost is reduced; the kit has extremely high specificity, sensitivity and repeatability, and the primers and the probes do not interfere with each other; good normal-temperature storage stability is also realized.

The present invention provides isolated Clostridium difficile binary toxin antibodies that are capable of neutralizing binary toxin with high potency, polypeptides and nucleic acids encoding the same, and methods of use thereof.

The invention relates to the field of microorganisms, and particularly discloses a clostridium butyricum microecological preparation composition for improving the sperm quality of livestock and poultry. The preparation method comprises the following steps: selecting materials such as cortex phellodendri to prepare mixed powder, using materials such as zinc-rich edible yeast powder to prepare a pasty bacterial colony proliferation culture medium, using materials such as the mixed powder and clostridium butyricum dry powder to prepare activated clostridium butyricum bacterial sludge, and performing mixed fermentation on the activated clostridium butyricum bacterial sludge and the pasty bacterial colony proliferation culture medium; and finally, pouring a mixed solution formed by xanthan gum, fructo-oligosaccharide and purified water into mixed dry powder of materials such as the bacteria-rich fermentation mixture, L-carnitine and the like to obtain a blend wet material, and drying the blend wet material to obtain the microecological preparation composition. The microecological preparation composition is added into the feed, so that the palatability and the nutrient utilization degree of the feed can be effectively improved, the ingestion and digestion of livestock and poultry are promoted, the organism immunity of the livestock and poultry is enhanced, and the semen quality of the livestock and poultry is improved on the premise of avoiding overdraft of the livestock and poultry.

The application discloses a method for fermenting crop straws by using composite microorganisms and application of the composite microorganisms in mine ecological restoration, and belongs to the technical field of agricultural waste resource utilization and environmental restoration. The composite microbial inoculum is composed of Clostridium coccoides, Bacillus plantarum and Acetobacter pasteurianus. The composite microbial inoculum has a significant synergistic effect in degrading the nutrient components of crop straws and accumulating fermentation products, and can obtain high-quality fermentation products. The fermentation products can improve soil structure and morphology, and reduce heavy metal activity, so that the effect of mine ecological restoration is achieved. Therefore, the composite microbial inoculum provides technical guidance for resource utilization of agricultural wastes and sustainable development of the environment. The fermentation products produced by fermenting crop straws by using the composite microbial inoculum provide a new way for mine ecological restoration.