The invention relates to chromium-free photo-sensitive resist for rotary screen printing, which comprises the following components: polyvinyl alcohol (30%), gelatin, p-diazodiphenylamine paraformaldehyde condensate, polyisocyanate blocks, silicone modified polyurethane resins, trimethylolethane, 3-6 parts of stilbazole dimethyl sulfate salt, hydroquinone, curing agents, polyethyleneimine, ethylamine boron trifluoride salt, benzyl dimethylamine, aluminum chloride hexahydrate, solvents, aluminum chloride hexahydrate, silicone defoaming agents and vitamin E. The chromium-free photo-sensitive resist has the advantages of strong binding force of the high-precision photo-sensitive resist for a rotary printing screen and the rotary printing screen, excellent chemical resistance and abrasion resistance, and high press run; plate making and developing of the rotary printing screen are clear and mesh holes cannot be stopped; through adding the silicone modified polyurethane resin, the tensile property of the rotary printing screen in a printing process is improved, and the service life of the rotary printing screen is prolonged.