332results about How to "No cross contamination" patented technology

A multifunctional scouring detergent with constituents (by weight percentage) of, mixed solvent 2-70, surface active agent 3-30, inorganic salt 0-10, water for the rest. The multifunctional cleaning agent can effectively remove the stains of chewing gum, scotch tape colloid residual and paint film coating.

The invention discloses a hanging tool for wafer electroplating. The hanging tool comprises an upper cover, a lower plate and a metal conductive ring, wherein an inner thread is arranged on the inner wall of the upper cover, a wafer groove is formed in the lower plate, an electroplating hole is formed in the middle of the wafer groove, and an outer thread engaged with the inner thread is arranged on the outer wall of the wafer groove; a platform is arranged between the inner wall of the wafer groove and the electroplating hole; the metal conductive ring is arranged on the platform. The upper cover of the hanging tool for the wafer electroplating is locked in a rotary mode, and the need of manually disassembling screws is eliminated, so that the hanging tool is convenient to operate, uniform in force, good in electrical conductivity and sealing performance and uniform in water coating, thereby greatly saving the manpower and reducing the wafer scrapping risk.

The invention relates to a method for detecting bisphenol A and bisphenol AF in food, belonging to technical field of analytical chemistry determination method. With ionic liquid (1-butyl-3-methylimidazole hexafluorophosphate) as an extractant and TritonX-100 or acetone as an dispersant, ultrasonic oscillation is carried out to form emulsion; after centrifugal separation, extraction drops at the lower layer are directly subjected to HPLC (high performance liquid chromatography) quantitative analysis. The method disclosed by the invention has the advantages of simpleness in detection steps, easiness in operation, low detection limit and high enrichment multiple, greatly reduced detection cost. The method disclosed by the invention is a rapid, efficient and environmentally friendly pre-treatment technology and has a wider application prospect in the field of food analysis.

The invention provides an independent primary air system provided with a heat pipe exchanger. The independent primary air system is characterized by comprising a precooling unit, a cooling dehumidification unit, a reheating unit and an air supply unit. The precooling unit, the cooling dehumidification unit, the reheating unit and the air supply unit are sequentially arranged between an inlet and an outlet of the primary air system; the air supply system is arranged on the outlet of the primary air system. Primary air is precooled through an evaporator side of the heat pipe exchanger; the preprocessed primary air is cooled again through the cooling dehumidification unit; the primary air is bent through an air pipe and then heated again through a condenser side of the heat pipe exchanger; the processed air is sent indoors through the air supply unit, and indoor temperature and humidity are controlled to be 26 DEG C + / -0.5 DEG C and 50%+ / -1%. The heat pipe exchanger of the independent primary air system achieves heat recovery in the precooling unit and the reheating unit, heat exchange efficiency is high, extra energy loss is avoided, and discharged heat can be recovered to the maximum; indoor air quality is improved; finally an air-conditioning system can save energy by 6%-9%.

The invention belongs to the field of immune detection and analysis, and particularly relates to a full-automatic micro-fluidic chip fluorescence immune detection system and a detection method implemented by the same. The full-automatic micro-fluidic chip fluorescence immune detection system comprises a shell and a main framework, and is characterized in that a chip card storage disc and a sample tube disc are fixed to the upper portion of the main framework, a centrifugal reaction disc is arranged between the chip card storage disc and the sample tube disc, and a filling system and a cleaning system are arranged between the centrifugal reaction disc and the sample tube disc; an optical detection system is arranged on the main framework and corresponds to the centrifugal reaction disc, a control system and motors are arranged inside the main framework, and the chip card storage disc, the centrifugal reaction disc, the sample tube disc and the filling system can be driven by the motors to rotate. The full-automatic micro-fluidic chip fluorescence immune detection system and the detection method have the advantage that requirements on full automation, multiple samples, high flux and quick detection can be fundamentally met by the full-automatic micro-fluidic chip fluorescence immune detection system.

The invention provides a method for simultaneously detecting 13 kinds of steroid hormones in serum. The method includes the following steps: a to-be-tested serum sample is mixed with an internal standard solution, a detection liquid is obtained by liquid-liquid extraction, and detection is performed by ultra-high-performance liquid chromatography-tandem mass spectrometry; liquid chromatography conditions are that: a mobile phase A is a formic acid aqueous solution of 0.1%, and a mobile phase B is a formic acid methanol solution of 0.1%; mass spectrometry conditions are that: a positive ion mode employs a multi-reaction monitoring mass spectrometry scanning mode; and the 13 kinds of steroid hormones include pregnenolone, progesterone, 17-hydroxypregnenolone, 17[alpha]-hydroxyprogesterone, 11[alpha]-hydroxyprogesterone, 21-deoxycortisol, 11-deoxycorticosterone, corticosterone, cortisol, 11-deoxycortisol, cortisone, aldosterone, and estrone. The method simultaneously detects the 13 kindsof steroid hormones with the detection time of only 6 minutes, and is easy to operate and takes less time; the sensitivity is high, the accuracy is good, the specificity is strong, a detection range is wide, and an application prospect in medical and biochemical detection fields is broad.

In order to enhance the durability of a rotor, provided is a flow channel switching valve which has a stator (11) with contact face (13) and a rotor (15) with contact face (17). The stator (11) on the contact face (13) has throughflow holes (19) to be connected to multiple flow channels, respectively. The rotor (15) has at least one groove (21) for communication between two of the throughflow holes (19) of the stator (11). The rotor is urged against the contact face (13) of the stator (11) and makes rotational slide so as to attain switching of the throughflow holes (19) of the stator (11) to be brought into communication. The contact face (13) of the stator (11) results from application of DLC coating and subsequent polishing processing. On the other hand, the contact face (17) of the rotor (15) consists of a resin.

The invention relates to a washing, bottling and corking device of glass bottle and similar container. Wherein, it comprises a bottler transporter, a bottle washer, a bottle corking machine, and a bottle discharge device, which are connected in serially; the bottle transporter is connected to the bottle charging device of bottler washer; a multi-track transporter is arranged between the bottle washer and the bottling corking machine; one end of multi-track transporter is connected to the bottle discharging device of bottle washer, while another end is connected to the bottling corking machine. The invention has simple structure and high producing efficiency.

The invention belongs to the field of micro-fluidic chips, and relates to a biological macromolecule detection method. An alpha-LISA technology and a droplet-based microfluidics technology are combined and optimized to prepare a micro-fluidic chip liquid. A novel biological macromolecule detection method with a high sensitivity is provided. A three-phase mixing technology is adopted. A passive mixing structure is used to fully and evenly mix the reaction liquid. A cross focusing technology is used to form droplets. The generated droplets have the advantage of smaller volume, and thus the reactions become faster. Moreover, the requirements on the samples are lower, the reactions are specific, sensitive, fast, and full; the washing does not need to be carried out after reactions; complicated complexes such as complete proteins, enzyme complex, bacteriophage, and the like, can be detected; the technical bottleneck of conventional commercial liquid chips at present can be broken through; the provided method can be applied to fast clinical biological macromolecule detection; the detection sensitivity and specificity are both improved, and the required sample amount is reduced.

The invention provides a manufacturing method of a unimolecular sequencing chip. The manufacturing method includes the following steps: (1), taking a baseplate, and adopting a photoetching method to make an anodic film of a reaction tank array on the surface of the baseplate; (2), using model glue to pour the anodic film, curing for 1-3h at 90-100 DEG C after vacuum degassing, uncovering the anodic film to obtain a groove with multiple runners, punching a hole in each of two ends of each runner to form fluid input and output holes, and obtaining a substrate; (3), taking a transparent base, and preparing a polymethylglutarimide (PMGI) layer on the surface of the transparent base to obtain a transparent base with the surface modified; (4), subjecting the substrate and the transparent base to oxygen plasma cleaning and press-fitting, and injecting a reagent into each runner to clean the corresponding PMGI layer contacting with each runner to obtain the unimolecular sequencing chip. The manufacturing method is simple in process and low in manufacturing cost.

The invention discloses a high-voltage electric pulse pretreatment method for intensifying the leaching of difficult-to-treat gold ore, and belongs to the technical field of the processing of minerals of gold ore. According to the method, the high-voltage electric pulse crushing pretreatment is realized by utilizing the differences of the dielectric properties of different minerals in ore; a high-voltage electric pulse discharge crushing device is used for carrying out selective crushing on gold-containing ore; gold and a gangue mineral in a gold-containing material are prompted to generate a micro crack and a running crack at an interface; a crystal of a gold-containing mineral is completely dissociated into monomer mineral particles; in this way, the exposed surface area of the gold is increased; therefore, the contact probability of cyanide and a gold grain is increased, so that the sufficient improvement of the leaching rate of the gold is facilitated.

The invention discloses a high-voltage electric pulse ore crushing device and a method for ore pre-treatment and belongs to the technical field of ore crushing pre-treatment in mineral processing. The device mainly comprises a power supply, a single-phase voltage regulator, an alternating-current ignition transformer, a six-time-voltage rectification circuit, an ultrahigh-voltage ceramic capacitor, a gas switch, a high-voltage electrode, a conductive barrel body, a discharging and ore crushing bucket, a vibration screen mesh, insulating liquid, a solid-liquid separator, a product collector and the like; all the units are fixedly connected through a lead wire and other auxiliary devices to form a complete equipment system. The ores are treated and then are selectively dissociated and crushed according to different conductive properties of useful metal minerals and non-metal mineral components of gangues, and extension cracks and fissures are generated on mineral interfaces on the ores, so that physical properties of the minerals are improved and subsequent treatment energy consumption is reduced; meanwhile, useful minerals are prevented from excessive abrasion and separation indexes are improved.

The invention discloses a fluid bed granulating drier applied in various fields such as pharmacy, food, feeds, pigments and chemical industry and a drying process thereof. The fluid bed granulating drier comprises an adhesive tank, an air compressor and a fluid bed, wherein a hot air inlet is formed in the lower half part of the fluid bed and is connected with a heater; an air outlet of the fluid bed is connected with an exhaust fan via a pipeline; a filter is connected with the upper half part of the heater and is provided with an air inlet; filter cloth and a muffler are arranged at the air outlet of the fluid bed. The drier has a simple structure, is reasonable in design, completes the processes of mixing, granulating and drying in the same equipment, is quick in mass heat transfer, is operated at closed negative pressure, thus avoiding dust flying, is uniform in granules, does not have dead angles, is cleaned thoroughly, is free from cross pollution and is energy-saving and environment-friendly.

The present invention provides a dispersive liquid-liquid microextraction method for pretreatment of plant hormone, i.e. DLLME is used for pretreatment of plant hormone. The method comprising the steps of: loading, mixing an extracting agent and dispersing agent, oscillating to form an opacifying system, centrifugal separation and drawing the organic phase to perform HPLC analysis directly. The method of the invention has high enrichment property, simple operation, less use of organic solvent and short extraction time, integrates the sampling, extraction and concentration, at the same time, avoids the potential problem of cross contamination in solid-phase microextraction, is a novel sample pretreatment technique with the advantages of simpleness, rapidness, low cost, high efficiency and environment protection, and has broad application prospect in the field of trace analysis.

The invention discloses a dawsonite-based polyvinyl chloride composite heat stabilizer and application thereof. The heat stabilizer consists of dowsonite, zinc soap and beta-diketone compound, and the proportion of each component by mass: 100 parts of dowsonite, 5 to 100 parts of zinc soap, and 1 to 100 parts of beta-diketone; the consumption volume of the heat stabilizer is that: 0.1 to 8 parts of heat stabilizer is added to every 100 parts of PVC resin. The composite heat stabilizer has the advantages that the raw materials are easy to obtain, the production process is simple, the cost is low, the heat stabilizer is toxic-free and environment-friendly, the compatibility is good, the PVC initial coloring can be effectively inhibited, the long-term heat stabilization efficiency is excellent, the transparency and the electric insulation property are excellent, and the like.

The invention discloses a method for quantitively detecting methane-oxidizing bacterium. The method comprises the following steps: sampling; washing bacterium with PBS (Phosphate Buffer Solution) by shaking samples; designing and selecting a double-layer microfiltration membrane plate with specific pores according to the number of the samples; and filtering the bacteria, passing the membrane and fixing; and conducting fluorescent dyeing and result analysis. According to the fluorescent strength of samples on the microfiltration membrane plate, the number of the methane-oxidizing bacterium in soil samples or sediment samples can be quantitively calculated, so as to provide technical support for microorganism oil-gas exploration and microorganism oil-gas reservoir characteristics.

The invention discloses a turntable type quantitative discharging device, which comprises a storage bin. The turntable type quantitative discharging device is characterized in that the storage bin is a barrel with a circular upper part and a conical lower part, the lower edge of the lower conical part of the barrel is fixedly connected with a discharging pipe provided with a serrated bottom edge, and the outer side of the discharging pipe is fixedly provided with a bin bottom flange plate with external threads. The turntable type quantitative discharging device also comprises a cylinder which supports a supporting point outside of the storage bin so that the storage bin can rotate; a turntable assembly which is connected with the threads of the bin bottom flange plate through threads and comprises a three-claw turntable seat, a turntable arranged in the middle of the turntable seat and a rotating beam barrel; a collecting tank which is arranged below the turntable assembly and is a barrel with an upper circular part and a lower conical part; a driving host which is arranged in the middle upper part of the collecting tank; and a weighing scale which is arranged in the middle upper part of the collecting tank. The device has the advantages of simple structure, stable discharge and easy use and cleaning, can realize the smooth, accurate, rapid and automatic quantitative discharging of trace raw materials, and is applicable to the mixing and production with multiple types of powdered and particular trace raw materials and high weighing precision.

The invention relates to a multifunctional bioreactor used for cell culture and cell sorting. The multifunctional bioreactor comprises a turnover device and a cell culture vessel, wherein the turnover device contains a turnover support which can operate around a space axis and a magnetic field generating device in physical connection with the turnover device, the cell culture vessel is detachably fixed on the turnover support, the cell culture vessel is located on the space axis or an extension line thereof and operates along with the turnover support according to certain rules and tracks, the end face of the cell culture vessel is close to the magnetic field generating device, and the magnetic field generating device can control change of a magnetic field surrounding the periphery and interior of the cell culture vessel. The multifunctional bioreactor provided by the invention has the advantages that co-culture on adherent cells and suspension cells is carried out without replacing the vessel, stirring inside a culture solution is realized by virtue of movement of carriers in the vessel in a magnetic field environment, cell contamination, target cell damage and death risk are reduced, and the vessel can be taken as a bearing material of the adherent cells, so that cell culture efficiency is greatly improved.

The invention provides a method for measuring the total content of nitrogen of fresh water quality. The method comprises the steps of sampling, preparing an alkaline potassium persulfate solution, preparing a potassium nitrate standard solution, preparing a hydrochloric acid solution, drawing a calibration curve and detecting a water sample to be measured. According to the method, a polytetrafluoroethylene decomposition bottle is adopted to replace a colorimetric tube, so that the decomposition process is relatively sealed and relatively fully carried out, and the decomposition time is relatively short; cross contamination is not caused between the inside and the outside of the decomposition bottle, and interference is eliminated, so that the result is relatively accurate, and the operation is relatively simple and relatively convenient; furthermore, an opening or a plug of the colorimetric tube is prevented from being broken in the decomposition process, so that the problem of analyzing by sampling again is avoided. Furthermore, after the colorimetric tube is replaced by the decomposition bottle, and a pipette is also used, the sample can be analyzed at the constant volume without being transferred into the colorimetric tube after being decomposed, so that the method is convenient, fast and easy to operate, and the accuracy is improved.

The invention provides a single-molecule sequencing chip, which comprises a substrate and a base layer which is arranged in a mode of being laminated with the substrate, wherein the substrate comprises a first surface and a second surface which are oppositely arranged; a reaction tank array which is formed by a plurality of runners is arranged on the first surface of the substrate in a spacing mode; two opposite sidewalls of each runner extend in the length direction of the runner and are intersected at two ends of the runner to form two conical tail ends having included angles; fluid input holes and fluid output holes which are communicated with the second surface of the substrate are respectively formed in the surfaces of the two conical tail ends; the base layer comprises a transparent base and a spacing layer which is arranged on the surface of the transparent base; and the spacing layer is contacted with the first surface of the substrate and the spacing layer, corresponding to the locations of the runners, is formed with corrosion grooves. The chip is good in flow field distribution, the base in the chip is low in deformation rate and fluid in the chip is thorough in flushing switching.

The invention discloses a combined oil mist purifying unit of an air conditioner. The combined oil mist purifying unit comprises an air return filtering part and a fresh air conveying part, wherein the oil return filtering part is used for performing suction filtering on air in a workshop and filtering oil mist particles and comprises an air return section, an oil mist filtering section, a peculiar smell purifying section, an air return fan section and an exhaust regulating section which communicate in sequence; the fresh air conveying part is used for feeding clean fresh air into the workshop and regulating the temperature of the air, and comprises a fresh air regulating section, an air filtering section, a temperature adjusting section, an air feeder section and an air feeding section which communicate in sequence; the exhaust regulating section and the fresh air regulating section communicate by an air mixing valve; and an exhaust valve and a fresh air valve are jointly arranged on a thermal recovery section. According to the combined oil mist purifying unit disclosed by the invention, by organically combining oil mist purification and the air conditioner, exhausting and thermal recovery can be realized; in addition, no cross contamination exists in the fresh air and the exhausted air; and the temperature and the air volume can be adjusted according to the technological requirements. The combined oil mist purifying unit has the functions of utilizing waste heat of the unit and purifying waste gases, and has the advantages of safety, reliability, reduction of pollution, convenience in technological operation and high degree of automation.

The invention discloses a fluid bed granulating drier applied in various fields such as pharmacy, food, feeds, pigments and chemical industry. The fluid bed granulating drier comprises an adhesive tank, an air compressor and a fluid bed, wherein a hot air inlet is formed in the lower half part of the fluid bed and is connected with a heater; an air outlet of the fluid bed is connected with an exhaust fan via a pipeline; a filter is connected with the upper half part of the heater and is provided with an air inlet; filter cloth and a muffler are arranged at the air outlet of the fluid bed. The drier has a simple structure, is reasonable in design, completes the processes of mixing, granulating and drying in the same equipment, is quick in mass heat transfer, is operated at closed negative pressure, thus avoiding dust flying, is uniform in granules, does not have dead angles, is cleaned thoroughly, is free from cross pollution and is energy-saving and environment-friendly.

The invention provides a chemical derivatization-ultra-high performance liquid chromatography-tandem mass spectrometry method for simultaneously detecting 18 steroid hormones in serum, which is characterized in that a novel derivatization reagent 3-pyridylmethoxylamine is adopted for derivatization and then ultra-high performance liquid chromatography-tandem mass spectrometry is used for detection. According to the method, only 10 minutes are needed for detecting 18 steroid hormones at the same time, the hydrophobicity index logP is greatly improved after derivatization, the peak shape and theseparation degree in a high performance liquid phase reversed phase chromatographic column are improved, and the ionization efficiency is better, so that the mass spectrum detection sensitivity is improved. The method has the characteristics of simplicity and convenience in operation, good accuracy, strong specificity, wide detection range and the like.

The invention discloses a collaborative quadruple real-time fluorescence PCR detection method for simultaneously detecting target nucleic acids of nine pathogens by using three PCR reaction tubes. Themethod is used for detecting influenza A virus (FluA), influenza B virus (FluB), parainfluenza virus (PIV), respiratory syncytial virus (RSV), mycoplasma pneumoniae (MP), chlamydia pneumoniae (Cpn),adenovirus (ADV), human rhinovirus (HRV) and human metapneumovirus (hMPV) in samples. The method is rapid to operate, can be completed by one-step cooperation and has high sensitivity. In addition, the invention also relates to a reagent involved in the PCR method, a detection kit used for the method, preparation and application of the detection kit and the like.

The method and system of Chinese traditional medicine flexibility electron formula, the character lies in: A. it separates Chinese traditional medicine and extracts effective component through using the heterogeneity multi-matter synchronization separation technology and other modern Chinese traditional medicine extraction technology; B. establishes the spectrum effect union Chinese traditional medicine effective component quality and the data bank through the pharmacodynamics experiment and the Chinese traditional medicine effective component extraction fingerprint atlas; C. produces the extracted material into standard quota Chinese traditional medicine particles through modern particle producing technology. D. puts the Chinese traditional medicine particles in the modular particle storage system, counts and quantifies the particle through control valve which is controlled by computer and photo-electricity gauge system; E. carries on the collection of medicine through the robot collection or conveyer belt.

The invention discloses a mass spectrometry method based on a single-pixel solid-phase extraction technology and application thereof. The mass spectrometry method comprises the following steps: (1) spraying a solvent for desorbing target molecules into a purified conical through hole in a spray form of a spray, enabling the spray to run through the conical through hole, desorbing the target molecules to obtain a secondary spray comprising the target molecules, enabling the secondary spray to enter a sample feeding port of a mass spectrometer so as to finish sample feeding of one conical through hole, and similarly finishing sample feeding of a next conical through hole so as to finish mass spectrometry on each conical through hole by the mass spectrometer; (2) performing sample collection and data analysis by the mass spectrometer so as to acquire a mass spectrum image of component ions. The mass spectrometry method adopts the single-pixel solid-phase extraction technology innovatively; through intra-pixel point pretreatment, an inhibiting phenomenon of a complex matrix on the ions of the target molecules is effectively avoided, so that influence of complex matrix interference on the accuracy of the mass spectrum image is practically avoided and the sensitivity of low-abundance component imaging is improved.

The invention discloses a high-voltage electric pulse selective pulverizing and pre-enriching method for lean hematite ores. The method is characterized in that high-voltage electric pulse pulverizing pretreatment is realized by utilizing dielectric property difference of different minerals in ores, energy with extremely high power density is output by high-voltage pulses, and the energy is discharged onto the lean hematite ores through a high-voltage electrode; valuable mineral hematite, gangue mineral quartz and the like in the ores form a plurality of micro cracks among interfaces, so that selective dissociation for hematite and gangue minerals is realized; and pre-enriched concentrate is obtained through strong magnetic separation. The method improves beneficiation feed grade of the ores, and is beneficial for improving follow-up grading and sorting indexes.

The invention provides a B19 (Human parvovirus B19), HTLV (Human T-cell lymphotropic virus) and HEV (Hepatitis E virus) quadruple fluorescent PCR (polymerase chain reaction) quick hypersensitivity detection kit and an application thereof, and relates to a real-time PCR method for quadruple detection of target nucleic acid in a nucleic acid extraction liquid in a single PCR reaction container. The method is used for detecting B19, HTLV-I, HTLV-2 and HEV.