The invention discloses an antibacterial anti-inflammatory and biomedical dressing for promoting wound healing and a preparation method of the dressing, and belongs to the technical field of medical supplies. The dressing uses a curcumin-containing calcium alginate fiber nonwoven fabric as a skin close layer, the curcumin-containing calcium alginate fiber nonwoven fabric can provide an ideal antibacterial environment for wound healing, promote wound healing, and relieve wound pain; the dressing uses a chitosan fiber non-woven fabric as a middle layer, and the chitosan fiber non-woven fabric has the effects of diminishing inflammation, promoting blood coagulation and inhibiting scars while absorbing exudates; the dressing uses a polyurethane film as an isolation layer, and the polyurethanefilm can achieve the effects of preventing dust, water, air leakage and pollution; and the biomedical dressing prepared by compounding the curcumin-containing calcium alginate fiber nonwoven fabric, the chitosan fiber non-woven fabric and the polyurethane film has complementary and synergistic effects, and the advantages of realizing antibacterial and bacteriostatic properties, a good anti-inflammatory effect and anti-adhesion performance, promoting rapid wound healing and having good biocompatibility. The medical hemostatic dressing provided by the invention has no side effects on human bodies and no any irritation to skin, is safe to use, environmentally friendly and pollution-free, and has wide application prospects in the field of biomedical materials.