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40 results about "Membrane complex" patented technology

Manufacturing method of high-energy-density button lithium ion battery

The invention discloses a manufacturing method of a high-energy-density button lithium ion battery. The method comprises the following steps: stamping: stamping a positive electrode and a negative electrode into small pieces by using cutting dies; hot melting: making positive pieces and membranes into a positive piece-membrane complex; laminating: alternatively laminating the positive piece-membrane complex and negative pieces into a polar group, and rubberizing the polar group; welding: laminating an aluminum foil of the same shape as the positive pieces on one side of the polar group which is in contact with a positive cover, welding the lug of the aluminum foil and the lugs of the positive pieces together, welding the lugs of the negative pieces and a nickel belt together, putting the materials into a shell, and welding the nickel belt and a negative shell together; and performing baking, liquid injection, sealing, ageing and pre-charging to make a complete button lithium ion battery. Compared with an existing process, the method has the advantages that the space of the shell can be fully utilized, and the energy density is high; and through adoption of the positive piece-membrane complex made by hot melting, short circuit caused by contact of the positive and negative pieces is prevented, and the safety is high.
Owner:SINOWATT DONGGUAN

Method for targeted capture and sequencing of HLA gene sequences

The invention relates to the field of biotechnology, and particularly provides a method for targeted capture and sequencing of HLA gene sequences. The method comprises the following steps: 1) hybridizing a denatured nucleic acid sample with a nucleic acid probe library fixed on a solid-phase carrier under a hybridization condition to form a target nucleic acid molecule-probe-solid-phase carrier membrane complex; 2) cleaning the 'probe-solid-phase carrier membrane complex' bound with target nucleic acid in step 1) by using a cleaning solution, eluting the probe-solid-phase carrier membrane complex bound with the target nucleic acid from a solid-phase carrier membrane by using an eluting solution, purifying, enriching or constructing to obtain a nucleic acid library; and 3) taking the nucleic acid library obtained by enrichment or constructed after enrichment in step 2) for high-throughput sequencing. A nucleic acid probe which can rapidly generate high-fold coverage and single-base displacement and is used for enriching HLA genes can be immovably fixed on the solid-phase carrier membrane. The method has characteristics of simplicity and convenience in operation, flexible probe acquisition and low cost, is favorable for improving the HLA genotype identification accuracy, and is rapid and convenient.
Owner:ZHEJIANG UNIV

Solid-phase-carrier-membrane-based target nucleic acid enrichment method for high-throughput sequencing

The invention relates to the field of biotechnology, and specifically provides a solid-phase-carrier-membrane-based target nucleic acid enrichment method for high-throughput sequencing. The solid-phase-carrier-membrane-based target nucleic acid enrichment method comprises: 1) combining a nucleic acid probe library and a solid phase carrier membrane to form a probe-solid phase carrier membrane complex; 2) adding target nucleic acid to a hybridization solution containing the probe-solid phase carrier membrane complex, and enriching the target nucleic acid; 3) washing the obtained probe-solid phase carrier membrane complex with the target nucleic acid by using a washing solution, eluting from the solid phase carrier membrane by using an eluent, and purifying the enriched target nucleic acid library; and 4) applying the target nucleic acid library enriched in the step 3) in high-throughput sequencing. According to the present invention, with the method, the high-multiple coverage and single-base displacement nucleic acid probe for enriching the target nucleic acid can be rapidly produced, and is immovably fixed on the solid phase carrier membrane; and the method has advantages of simple operation, flexible probe acquisition and low cost.
Owner:朱静
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