The invention belongs to the technical field of biological composite materials, and particularly relates to a CE-SKP / CPH / P2G3 three-layer
composite scaffold, a preparation method and application. The CE-SKP / CPH / P2G3 three-layer
composite scaffold is characterized in that CPH hydrogel is placed under a CE-SKP
composite scaffold system, a P2G3
nanofiber membrane is placed under the CPH hydrogel, a calcified
cartilage layer of a physiological structure is simulated, relative independence of a
cartilage repair microenvironment is kept, and mineralization of newborn
cartilage cells is induced, so that the calcified cartilage layer of the physiological structure is formed; wherein the CE-SKP composite
scaffold system is a seed
cell-free composite
scaffold system which is constructed by adsorbing self-assembled polypeptide
nanofiber hydrogel Ac-(RADA) 4-CONH2 / Ac-(RADA) 4GGSKPPGTSS-CONH2 (RAD / SKP) to a natural cartilage
extracellular matrix (ECM), and the composite
scaffold system is a seed
cell-free composite scaffold system which is constructed by adsorbing the self-assembled polypeptide
nanofiber hydrogel Ac-(RADA) 4-CONH2 / Ac-(RADA) 4GGSKPPGTSS-CONH2 (RAD / SKP) to the natural cartilage
extracellular matrix (ECM). The CE-SKP / CPH / P2G3 three-layer composite scaffold utilizes the three-layer
composite structure to solve the technical problems that in the prior art, a cartilage layer and a calcified cartilage layer in a physiological structure cannot be well simulated, and independence of a repairing microenvironment cannot be kept, joint repairing of the cartilage layer and the calcified cartilage layer is achieved, and a better
articular cartilage full-thickness defect repairing effect is achieved.