Preparation method of dehydrogenase-electrochemical biosensor

A biosensor and dehydrogenase technology, applied in the field of electrochemical biosensor preparation, can solve the problems of many electrode modification steps, inability to rapidly mass-produce sensors, etc., to simplify the preparation process, improve storage stability and detection accuracy. Effect

A biosensor and dehydrogenase technology, applied in the field of electrochemical biosensor preparation, can solve the problems of many electrode modification steps, inability to rapidly mass-produce sensors, etc., to simplify the preparation process, improve storage stability and detection accuracy. Effect

CN102998348AInactive Publication Date: 2013-03-27CHONGQING MEDICAL UNIVERSITY
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  • Preparation method of dehydrogenase-electrochemical biosensor

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Embodiment Construction

[0022] The present invention will be further described below in conjunction with the examples. It should be understood that the following examples are only used for the purpose of illustration, and in no way limit the protection scope of the present invention.

[0023] The present invention takes the alcohol dehydrogenase type biosensor as an example to introduce the preparation method of the dehydrogenase type biosensor in detail.

[0024] The nanobiocomposites were prepared according to the following ingredients and amounts, and used to prepare alcohol dehydrogenase-based biosensors:

[0025] Graphitized mesoporous carbon (Sigma) 2mg / ml,

[0026] Meldora blue (Sigma) 0.1mM,

[0027] Chitosan (Sangon) 0.5wt.%,

[0028] Alcohol dehydrogenase (Sigma) 4mg / ml,

[0029] coenzyme NAD + (BBI) 4mM,

[0030] Tris-HCl buffer (Beyotime) 50~200mM;

[0031] The preparation steps of the nanobiocomposite and the preparation of the ethanol sensor are as follows:

[0032] 1) Under stir...

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Abstract

The invention relates to a preparation method of an electrochemical biosensor, and particularly relates to a preparation method of a dehydrogenase-electrochemical biosensor. The method comprises the following steps of: preparing nano biocomposite from all components (including a carbon nanomaterial, an electronic mediator, a high-molecular polymer, dehydrogenase, coenzyme and buffer solution) required for modifying electrodes in a preparation process of the sensor; and modifying the composite material to the surface of a screen-printed electrode by a simple one-step dispensing method, so as to prepare the dehydrogenase-electrochemical biosensor. By adopting the method, the preparation process of the dehydrogenase-electrochemical biosensor is simplified, and the method is suitable for mass production; an inter-batch difference and the difference between batches are reduced; and the detection sensitivity and accuracy and the storage stability are improved.

Description

technical field [0001] The invention relates to a preparation method of an electrochemical biosensor, in particular to a preparation method of a dehydrogenase type biosensor. Background technique [0002] With the rapid development of society and the significant improvement of people's living standards, the traditional clinical testing mode, due to the existence of: lack of effective pre-analysis quality control from specimen collection to testing, long sample testing cycle, high dependence on instruments, etc. Insufficient, it can no longer meet people's increasing demand for health assessment. With the rapid advancement of technology, the POCT (Point of Care Test) industry has radiated new vitality. Due to the outstanding advantages of rapid detection, simple operation and low cost of detection, POCT accounts for an increasing proportion of clinical testing efficiency. In recent years, an important branch in the field of POCT, electrochemical biosensor, as a new technolo...

Claims

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Application Information

Patent Timeline
27 Mar 2013
Publication
CN102998348A
IPC
G01N27/26; G01N27/30
Inventors
花尔辉; 谢国明