Preparation method of dehydrogenase-electrochemical biosensor

Abstract

The invention relates to a preparation method of an electrochemical biosensor, and particularly relates to a preparation method of a dehydrogenase-electrochemical biosensor. The method comprises the following steps of: preparing nano biocomposite from all components (including a carbon nanomaterial, an electronic mediator, a high-molecular polymer, dehydrogenase, coenzyme and buffer solution) required for modifying electrodes in a preparation process of the sensor; and modifying the composite material to the surface of a screen-printed electrode by a simple one-step dispensing method, so as to prepare the dehydrogenase-electrochemical biosensor. By adopting the method, the preparation process of the dehydrogenase-electrochemical biosensor is simplified, and the method is suitable for mass production; an inter-batch difference and the difference between batches are reduced; and the detection sensitivity and accuracy and the storage stability are improved.

Description

technical field [0001] The invention relates to a preparation method of an electrochemical biosensor, in particular to a preparation method of a dehydrogenase type biosensor. Background technique [0002] With the rapid development of society and the significant improvement of people's living standards, the traditional clinical testing mode, due to the existence of: lack of effective pre-analysis quality control from specimen collection to testing, long sample testing cycle, high dependence on instruments, etc. Insufficient, it can no longer meet people's increasing demand for health assessment. With the rapid advancement of technology, the POCT (Point of Care Test) industry has radiated new vitality. Due to the outstanding advantages of rapid detection, simple operation and low cost of detection, POCT accounts for an increasing proportion of clinical testing efficiency. In recent years, an important branch in the field of POCT, electrochemical biosensor, as a new technolo...

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Problems solved by technology

For example, Zhu et al. modified carboxylated carbon nanotubes dispersed in N,N-dimethylformamide to the surface of glassy carbon electrodes, then immersed them in Meldola blue solution, and adsorbed them for a period of time to obtain carbon nanotubes / Meldola blue modified electrode, the sensor exhibits high sensitivity and wide linear range to the reduced coenzyme NADH (Biosensors and Bioelectronics.2007, 22: 2768-2773); Zheng et al. The screen-printed electrode modified by -117 composite film was immersed in the Meldola blue solution for a period of time, and the carbon nanotube / nano-gold / Meldola blue / Nafion-117 composite film modified electrode was obtained, and the composite film electrode was used successfully Alcohol dehydrogenase-type sensors were prepared, and the obtained electrodes showed excellent stability and accuracy (ForensicScience International. Shortcomings for rapid mass production

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