90 results about "Screen printed electrode" patented technology

The invention provides construction of an electrochemical biosensor based on a fucoidin hydrogel microneedle and application of the electrochemical biosensor in online monitoring of uric acid, and relates to the technical field of biosensors. According to the micro-needle, Gantrez S-97 and PEG are subjected to copolymerization crosslinking to form a hydrogel main body, fucoidin is introduced for blending modification, a three-dimensional network structure is constructed by utilizing a high-temperature esterification reaction for regulating and controlling pH, and the super-expansion hydrogel micro-needle with high swelling property and good biocompatibility is formed. The microneedle can rapidly collect a skin interstitial fluid sample, and then through a screen printing electrode (SPE) platform, carboxylated MWCNTs are used for modifying an electrode and immobilizing uricase to construct an enzyme sensor. Hydrogen peroxide generated by catalyzing uric acid on the surface of the electrode participates in electrochemical reaction, and an output signal is used for real-time detection of uric acid concentration. The device integrates minimally invasive sampling and high-sensitivity electrochemical detection, has the advantages of quick response, simplicity and convenience in operation, good biocompatibility and the like, and is suitable for application scenes of continuous monitoring of uric acid, chronic disease management and the like.

A wearable diagnostic electrocardiogram (ECG) garment is disclosed herein. The wearable diagnostic ECG garment comprises a garment body, screen-printed electrodes positioned on the body, and screen-printed wires positioned in the garment body, each of the screen-printed wires connected from a central connector module to a screen-printed electrode.

The utility model discloses a metformin hydrochloride electrochemical analysis device which comprises a shell, and one side of the shell is provided with an electric sensor interface; the power supply unit is arranged in the shell and is used for generating polarization voltage; the silk-screen printing electrode electric sensor is electrically connected with the power supply unit and comprises a flexible electrode base body, a working electrode, a counter electrode, a reference electrode, an insulating layer, a wiring terminal and an electrode connecting wire, and the working electrode, the counter electrode, the reference electrode, the insulating layer, the wiring terminal and the electrode connecting wire are arranged on the flexible electrode base body; the surface of the working electrode is modified with an imprinted polymer with selective recognition on metformin hydrochloride; the signal processing unit is arranged in the shell and comprises a shunt resistor, an operational amplifier, a signal converter, a data storage chip and a microprocessor which are electrically connected in sequence; the display screen is electrically connected with the microprocessor and is used for displaying the converted concentration value; the method has the advantages that the metformin hydrochloride can be rapidly and accurately detected, and the use is relatively convenient.

The invention belongs to the technical field of electrochemical analysis and detection, and particularly relates to an electrochemical immunosensor for detecting adiponectin as well as a preparation method and application of the electrochemical immunosensor. The preparation method comprises the following steps: (1) connecting a screen-printed electrode SPCE to an electrochemical workstation by using a 0.01 M PBS (Phosphate Buffer Solution) with the pH value of 7.4, performing-0.2 V-1. 2V cyclic voltammetry scanning, and activating for 10 circles under 50mV / S; and (2) by taking 0.01-1M H2SO4 as a base solution, connecting the screen-printed electrode SPCE to an electrochemical workstation, carrying out electro-polymerization on poly-o-phenylenediamine in a 50-100mM o-phenylenediamine and NH2-UiO-66 solution to form a poly-o-phenylenediamine and NH2-UiO-66 composite modified electrode, washing with deionized water, and drying at room temperature. (3) soaking the modified electrode in a glutaraldehyde solution for 10-70 minutes, cleaning with deionized water, drying at room temperature, adding 10-100 [mu] L of ADPN antibody solution and 100-600 [mu] g / mL of ADPN antibody solution, and incubating; and (4) washing with ionized water, drying at room temperature, preventing non-specific binding sites at 37 DEG C for 0.5-1.5 hours by using 3-10 microliters of 0.8-1.2 wt% casein solution, and washing the screen-printed electrode again to obtain the electrochemical immunosensor.

The invention relates to a surface modified Fe / Cu-Co3O4 catalyst for nitrite detection and a preparation method thereof, a sensor and application, and the preparation method comprises the following steps: dissolving a cobalt source, an iron source, a copper source, urea and NH4F in deionized water, carrying out a hydrothermal reaction, washing, drying and calcining to obtain a Fe and Cu co-doped Co3O4 nanotube; and pre-hydrolyzing by using a KH792 silane coupling agent ethanol-water solution, and reacting with nanotubes to prepare the surface modified catalyst. The sensor takes carbon cloth or a screen-printed electrode subjected to ultrasonic cleaning and plasma treatment as a substrate, catalyst ink is coated to form a catalytic layer, and the catalytic layer, a platinum counter electrode and a silver / silver chloride reference electrode form a three-electrode system. Through molecular modification and material design, the interfacial force between the catalyst and nitrite is improved, the electrode stability is enhanced, and the catalyst is applied to a disposable printed electrode and portable detection equipment, so that high-sensitivity, stable and portable detection of nitrite is realized.

The application provides an electrochemical sensing ELISA reagent card, comprising a microfluidic channel body and an electrochemical sensor body which are bonded together, wherein: a plurality of "annular" liquid flow channels are arranged on the microfluidic channel body, each "annular" liquid flow channel is formed by a working electrode covering flow channel and a counter electrode and a reference electrode covering flow channel in series, and the "annular" liquid flow channels are communicated through a common flow channel; the electrochemical sensor body comprises a flat plate substrate and a plurality of screen-printed electrodes which are printed in parallel on the flat plate substrate, and each screen-printed electrode comprises a working electrode, a counter electrode and a reference electrode. The electrochemical sensing ELISA reagent card combines an electrochemical biosensor and a microfluidic chip, and solves the technical problems of large sample / reagent consumption, single detection index and liquid volume influence on absorbance measurement of a traditional ELISA reagent kit.

The invention relates to the technical field of electrochemical sensing, in particular to a multi-channel electrochemical aptamer sensor and a preparation method and application thereof.The multi-channel electrochemical aptamer sensor adopts a commercial silk-screen printing electrode and is combined with a polymer substrate material for amplifying electrochemical signals, and the electrochemical aptamer sensor is prepared. According to the present invention, the corresponding aptamer is fixed by using the gold nanoparticle through the chemical bond so as to specifically detect the three hemorrhage conversion biomarkers after acute cerebral infarction, such as human matrix metalloproteinase (MMP-9), fibrinogen (FN) and plasminogen activation inhibitor (PAI-1); wherein the FN and the PAI-1 are detected through an electrochemical aptamer sensor method for the first time, and simultaneous detection of the three biomarkers is realized through integration with commercial multi-channel micro electrochemical equipment.

The invention is applicable to the technical field of biological detection, and provides a method for detecting staphylococcus aureus based on combination of a screen-printed electrode and CRISPR / Cas13a, a probe containing a DNA / RNA hybrid chain is combined with a magnetic bead to form a simple composite structure, when the Cas13a recognizes 16sRNA of S. aureus, trans-cleavage activity can be immediately activated, the probe chain is rapidly cut off, the cut-off amount depends on the activated amount, and when the Cas13a recognizes 16sRNA of S. aureus, the Cas13a can be used for detecting staphylococcus aureus. And after transient magnetic separation, a result is observed on the screen-printed electrode. The method has ultrahigh specificity: the CRISPR / crRNA sequence ensures that staphylococcus aureus and other microorganisms can be accurately distinguished; the trans-cutting of Cas13a provides chemical amplification, and the magnetic separation purification greatly reduces background signals caused by non-specific adsorption, so that the signal-to-noise ratio and the sensitivity are remarkably improved.

The application discloses a self-driven disc type blood separation and detection device and method, which comprises a sample adding layer, a separation layer and a detection layer, and the layers are sequentially connected through screw fixation. The sample adding layer is provided with a sample adding port and a first screw hole; the separation layer comprises four repeated blood separation units and a second screw hole, and each separation unit comprises a sample inlet, a micro-fluidic channel, a filter groove, a sample outlet and a waste liquid collecting chamber; and the detection layer is provided with a screen-printed electrode interface and a third screw hole. Blood samples are dripped into the device from the sample adding port, and under the driving of the internal negative pressure of the device, the blood samples enter the micro-fluidic channel, and blood filtration is realized by using the gravity difference between blood cells and blood plasma. The filtered blood plasma flows into the detection layer and contacts with the screen-printed electrode detection unit, so that electrochemical detection is realized. The device does not need external power supply, thereby reducing sample operation, reducing the volume of the separation device, improving the blood separation efficiency, and facilitating instant detection and multi-channel detection of blood samples.

The invention discloses a portable electrochemical biosensor for detecting vibrio parahaemolyticus, the principle of the invention can be also suitable for detection of other pathogenic microorganisms, and the portable electrochemical biosensor comprises a PVC substrate, a sample pad, a capture pad, a screen printing electrode and a water absorption pad. According to the invention, the advantage of high sensitivity of electrochemistry and the advantage of no separation of the test strip are combined together, the vibrio parahaemolyticus is captured by using the aptamer and is fixed on the detection line, the signal probe is dropwise added, the electrochemical workstation is used for detection, and quantitative detection of the vibrio parahaemolyticus is realized by adopting a signal subtraction output mode. The method has the advantages of low cost, high detection speed, high sensitivity, simplicity in operation and the like. The requirements of customers for on-site rapid detection can be met, and food poisoning of patients can be effectively prevented.

The invention provides a method for rapidly detecting pesticide residues in fruits and vegetables, and belongs to the field of pesticide analysis and detection. The method comprises the following steps: mixing a copper nanoparticle / reduced graphene oxide / chitosan composite material, acetylcholin esterase, choline oxidase and a cross-linking agent glutaraldehyde solution for reaction to obtain a double-enzyme functionalized nano composite material; dispensing a chitosan solution on the surface of a working electrode of a screen-printed electrode, drying to form a film, and covalently modifying the surface of the electrode with the bienzyme functionalized nano composite material through a cross-linking agent glutaraldehyde to obtain a sensing electrode; according to the method, acetylcholine is taken as a substrate, a sensing electrode is used, a chronoamperometry is adopted, inhibition ratios of pesticide standard substance solutions with different known concentrations to electrode response current are detected, and a standard curve between the pesticide concentration and the current inhibition ratio is established; and calculating the pesticide residue concentration in the fruit and vegetable sample to be detected according to the current inhibition ratio of the liquid to be detected and the standard curve. Therefore, the sensitivity and accuracy of pesticide residue detection in fruits and vegetables are improved.

The invention discloses a preparation method of an electrochemical immunosensor for detecting adiponectin and a method for quantitatively detecting the adiponectin by adopting the electrochemical immunosensor, provides a new strategy for fixing an adiponectin antibody by a one-step method, and constructs the electrochemical immunosensor based on a gold sputtering silver wire screen printing electrode (Au / AgSPE). The rapid, high-sensitivity and stable detection of the adiponectin is realized, so that the conversion of the adiponectin detection technology to instant detection application is promoted. Only a portable electrochemical workstation is needed in the detection process, and large biochemical analysis equipment is not needed. And the screen printing electrode and the sputtering technology support large-scale production, and cost reduction and popularization are facilitated.

The invention discloses a method for detecting the concentration of acetic acid in an anaerobic fermentation system by using a bioelectrochemical sensor, acetic acid is detected by using the bioelectrochemical sensor, the bioelectrochemical sensor comprises a screen-printed electrode, and the working surface of the screen-printed electrode is modified with reduced graphene oxide and polymethylene blue. The working surface of the screen printing electrode is connected with acetokinase, pyruvate kinase and lactic dehydrogenase through a cross-linking agent. According to the method, the adopted sensor has better conductivity, higher response speed and higher current intensity, when the sensor is used for detecting the concentration of acetic acid in a fermentation system, the sensor has the advantages of being easy to operate, low in sample consumption, low in price, high in accurate measurement precision, high in detection speed and the like, and rapid detection of the concentration of acetic acid can be achieved; the acetic acid concentration in the anaerobic fermentation process in different periods can be timely and accurately obtained, effective data can be provided for anaerobic fermentation analysis, and the method has important significance for monitoring the process stability of anaerobic fermentation.

The invention provides a molecular classifier for detecting gastric cancer miRNA. A preparation method of the molecular classifier comprises the following steps: depositing a PABA modification layer on the surface of a screen-printed electrode; activating carboxyl on the surface of the para aminobenzoic acid modification layer by adopting an EDC / NHS composite system; carrying out amination modification on an MXene material, and coating the surface of the activated para aminobenzoic acid modification layer with the modified MXene material to form an MXene functional layer; depositing gold nanoparticles on the surface of the MXene functional layer to obtain an electrochemical activity sensing interface; a signal reporter molecule is combined with an aptamer probe, the signal reporter molecule is a DNA tetrahedron-methylene blue complex, and the combined complex is dropwise added on the surface of an electrochemical activity sensing interface, so that the aptamer probe is combined on the surface of the electrochemical activity sensing interface. The molecular classifier can be used for preparing gastric cancer miRNA detection products, and the problems that in the prior art, a classifier is high in complexity and high in signal leakage risk are solved.

The invention discloses a high-sensitivity Tau-441 electrochemical immunosensor based on a nano material synergistic amplification effect as well as a preparation method and application of the high-sensitivity Tau-441 electrochemical immunosensor. According to the sensor, a silk-screen printing electrode (SPE) is used as a substrate, a PEDOT: PSS / MWCNTs-COOH nanocomposite modified working electrode is used as a sensing platform, and electron transfer is promoted by using the large specific surface area and excellent conductivity of the PEDOT: PSS / MWCNTs-COOH nanocomposite modified working electrode. Meanwhile, gold-modified magnetic nanoparticles (Fe3O4-coated AuNPs) are prepared as a signal probe, and the magnetic separation and enrichment function of Fe3O4 and the biocompatibility and catalytic activity of the gold nanoparticles are combined. The sandwich immune complex is constructed by adopting a homogeneous reaction mode, so that the mass transfer resistance is effectively reduced, and the detection time is shortened. The sensor has an extremely low detection limit (19.68 fgmL <-1 >) and a wide linear range on an Alzheimer's disease marker Tau-441, is high in specificity and good in stability, and is suitable for early-stage rapid screening of human plasma samples.

The invention discloses a Ti3C2Tx-S / SPCE sensing electrode and a method for simultaneously detecting Cd < 2 + >, Pb < 2 + >, Cu < 2 + > and Hg < 2 + >. The electrode is mainly prepared by loading Ti3C2Tx-S on a screen-printed electrode (SPCE), and the Ti3C2Tx-S is prepared by carrying out one-pot etching and in-situ cation auxiliary intercalation on Ti3AlC2 MXene, according to the method, the lattice spacing and the specific surface area of the Ti3C2Tx-S material are increased, and more active sites are exposed; the detection limits for simultaneously detecting the heavy metals Cd < 2 + >, Pb < 2 + >, Cu < 2 + > and Hg < 2 + > are 3.1 nmol / L, 3.6 nmol / L, 5.2 nmol / L and 3.7 nmol / L in sequence; ti < 3 > C < 2 > T < x-S > shows good anti-interference performance, stability and repeatability; the electrochemical performance of Ti3C2Tx-MXene is improved by adopting a cation in-situ intercalation strategy, and the detection sensitivity of heavy metal ions such as Cd < 2 + >, Pb < 2 + >, Cu < 2 + > and Hg < 2 + > is improved.

The application provides a paper-based molecular imprinting sensor for detecting glucocorticoids and a preparation method thereof, wherein the preparation method of the paper-based molecular imprinting sensor for detecting glucocorticoids comprises the following steps: using nitrogen-doped carbon / carbon nanotube composite material with a hierarchical structure as electrode modification material, and coating the electrode modification material on a screen-printed electrode; and assembling the obtained screen-printed electrode with a paper-based microfluidic device. The nitrogen-doped carbon and the carbon nanotube form a hierarchical porous structure, and high specific surface area and high conductivity make the sensor performance more excellent. The paper-based microfluidic process enables the sensor to simultaneously detect multiple glucocorticoids, and shows the application value of the sensor in the inspection field.

The invention relates to the field of medicine, namely to biosensor analytical devices and can be used for quantitatively assessment of glucose content in blood, plasma and serum. The device comprises a graphite screen printed electrodes, whose surface is modified by a hybrid composite comprising an organosilicon matrix and inclusions of a biorecognition element in the form of glucose oxidase, as well as inclusions of carbon nanotubes and polyvinyl alcohol placed in the matrix. The organosilicon matrix consists of (3-aminopropyl)triethoxysilane and tetraethoxysilane in volume ratios of 20:80 and is covalently bound to a Nile blue mediator. The invention has the technical result of an increased upper limit of detectable glucose concentrations in physiological fluids up to 1.4 mmol / dm3.

The invention discloses a method for rapidly detecting inorganic arsenic in aquatic animals. The method comprises the following steps: pre-treating an aquatic animal sample to be detected to obtain a sample solution to be detected; the method comprises the following steps: taking a silk-screen printing electrode as a working electrode, electrifying a to-be-detected sample solution by adopting anodic stripping voltammetry to collect an electrical response parameter in the electrifying process, and further obtaining the content of inorganic arsenic according to the electrical response parameter. According to the method, the screen-printed electrode is adopted to replace a traditional glassy carbon electrode or gold electrode, polishing is not needed, operation is easy, throwing is achieved when the electrode is used, the price is low, batch production is easy, and the performance is stable, and the electrochemical anodic stripping voltammetry is applied to detection of inorganic arsenic in aquatic animals for the first time; the shape of the working electrode and an ink carbon-based material in the working electrode are optimized, and the quantitation limit of the finally measured sample is as low as 0.1 mg / kg; the method is simple, rapid and efficient, and is suitable for large-scale production and application on a base layer.

The present application relates to a high-sensitivity flexible electrochemical sensor and its preparation method and application, the preparation method comprises the following steps: step S1: preparation of NS-TiO2@M-HG composite material; step S2: preparation of rGO modified silk screen printing electrode rGSPE; step S3: NS-TiO2@M-HG composite material is modified on the working electrode of rGSPE, and NS-TiO2@M-HG / SPE is obtained. Compared with the prior art, the sensor array has a wide detection range, low detection limit and high sensitivity, while maintaining mechanical flexibility, anti-interference ability and repeatability. Through in-situ sweat biomarker detection during exercise, the sensor effectively tracked the fluctuations of ascorbic acid (AA), dopamine (DA) and uric acid (UA) levels in the volunteers. This practical application highlights the potential of the sensor in continuous health monitoring, early disease detection and personalized medical monitoring, making it a promising tool in the field of modern healthcare.

The application discloses a one-step monoclonal antibody drug electrochemical detection biosensor and a preparation method and application thereof. The application designs and screens an affinity specific DNA aptamer hairpin probe and uses the same as a specific capture unit, then uses a gold-sulfur bond to fix the DNA aptamer hairpin probe on a screen-printed electrode surface, meanwhile, uses a self-assembly interaction between a metal and a mercapto group to fix a blocking agent introduced on the screen-printed electrode surface, and blocks the excess electrode surface reaction sites, so that the one-step monoclonal antibody drug electrochemical detection biosensor is obtained. The one-step monoclonal antibody drug electrochemical detection biosensor has the advantages of simple operation, high sensitivity, less non-specific adsorption, high capture efficiency, stable physical and chemical properties, strong specificity and good selectivity, and can be used for capturing antibody drugs in complex biological samples.

The application discloses a method for detecting lead and cadmium in grain and oil based on a screen printing electrode of MWCNTs / NiO NPs doped carbon paste, and belongs to the technical field of electrochemical sensors and food safety detection. The application dopes multi-walled carbon nanotubes and nano nickel oxide into conductive carbon paste in the form of a predispersion, carries out three-roller grinding homogenization treatment, obtains modified conductive carbon paste, and adopts one-step screen printing process to prepare a working electrode without secondary modification. The working electrode of the application can be used for simultaneously detecting Pb 2+ and Cd 2+ , the detection limits are 0.5 ug / L and 0.77 ug / L respectively, the batch relative standard deviation of the working electrode is 92%, and the inter-batch RSD is 2.1%. In addition, the preparation method of the working electrode is simple, has good reproducibility, and can be mass-produced.

The present application belongs to the field of wearable sensor, and discloses a preparation and application of a sweat ascorbic acid sensor with simple structure and low cost, which adopts silk screen printing and wood substrate. The sensor of the present application combines silk screen printed electrodes and hydrophilic wood substrate. In order to overcome the shortcomings of past sensors, such as hydrophobicity and high cost, the silk screen printing technology is adopted for manufacturing. The silk screen printed carbon electrode has high flexibility and good conductivity, and the hydrophilic wood paper is used as the substrate, which has high ability of collecting, storing and transporting sweat. The sensor has the characteristics of high sensitivity (0.032 μA L μmol ‑1 ), high selectivity, low detection limit (10 μA L μmol ‑1 ), wide linear range (10-1000 μmol / L), good repeatability and good stability. In general, the wearable sensor developed in this study shows great potential in the prognosis, diagnosis and treatment of ascorbic acid disease, and is a promising and cost-effective strategy.

This invention belongs to the field of rapid detection technology, specifically relating to a rapid, real-time detection method for influenza A based on electrochemical biosensors. This invention provides an aptamer that specifically recognizes the influenza A virus, and uses a pairing affinity reagent to enrich the aptamer onto magnetic beads to prepare a probe that specifically recognizes the influenza A virus. Then, using this probe, MB solution, and screen-printed electrodes, an electrochemical biosensor that specifically recognizes the influenza A virus is fabricated. Qualitative or quantitative detection of influenza A virus using the electrochemical biosensor of this invention eliminates the need for complex pretreatment and viral nucleic acid amplification, greatly simplifying the operation. The results are stable and reliable, making it suitable for real-time detection applications.

The invention relates to a method for producing an electrode-coated cell layer (100) for an electrochemical cell stack, in particular a fuel cell stack or an electrolysis cell stack, and at least one catalyst-containing electrode (120, 130), in particular an anode electrode (120) and / or a cathode electrode (130), for the cell stack, is applied to a cell stack substrate (110), wherein an electrode coating material is printed onto a large-area outer surface of the cell stack substrate (110) by means of a screen printing process as a screen-printed electrode (120, 130) patterned in two length dimensions and structured in its thickness dimension (D).

The invention relates to a molecularly imprinted field effect transistor sensor and a preparation method and application thereof, and a screen-printed electrode assembly comprises a substrate, and a carbon working electrode and a silver / silver chloride reference electrode which are oppositely arranged on the substrate; a gold nanoparticle layer, a polydopamine connecting layer, a beta-cyclodextrin main body layer and an ionic liquid doped poly-o-phenylenediamine molecular imprinting layer are sequentially overlaid on the carbon working electrode. Compared with the prior art, the invention provides a molecular imprinting strategy based on beta-cyclodextrin orientation, accurate pre-orientation of a perfluoroalkyl substance (PFAS) template molecule is realized by using a hydrophobic cavity of beta-cyclodextrin through a host-guest inclusion effect, the uniformity and specificity of an imprinting cavity are effectively improved, and the accuracy and the specificity of the imprinting cavity are improved. The core problem that recognition sites are disordered due to random distribution of templates in a traditional molecular imprinting technology is solved.

The application discloses a kind of based on carboxylated multi-walled carbon nanotube modified silk screen printing electrode preparation azide ion electrochemical sensor.The application uses carboxylated multi-walled carbon nanotube as modification material, acetic acid solvent, and electrode modification liquid of 2mg / mL is prepared, 2 μL is dropped on the working carbon electrode of silk screen printing electrode, and N3 ‑ Electrochemical sensor. The concentration of azide ion in the solution is detected by electrochemical analysis method, and the sensor has the advantages of easy preparation, portability, sensitivity, low price and small required detection amount.

The invention belongs to the technical field of biosensing and underwater detection, and particularly relates to an underwater adenosine triphosphate detection system and method. According to the system, a nanogold modified screen-printed electrode is used as a sensing platform, based on an electrochemical aptamer sensing principle, an ATP specific nucleic acid aptamer is fixed in an Au-S bond self-assembly mode to form a molecular recognition interface, and when ATP is combined with the aptamer, characteristics such as interface electron transfer resistance are changed, so that the ATP specific nucleic acid aptamer is identified. The high-sensitivity and high-selectivity detection of ATP is realized by detecting the peak current change of the electroactive reporter molecule; meanwhile, an epoxy resin-silicone rubber-silane coupling agent composite packaging system is adopted, a compact protection layer is formed in a non-working area of the electrode through a stepped heating curing process, and the three parts cooperate to ensure the sealing performance and long-term stability in an underwater environment, so that underwater in-situ detection in a real sense is realized; the target object degradation in the sample collection and storage process is avoided. The method has important value in the fields of marine environment monitoring, underwater biological research and the like.

The invention provides a colorimetric / electrochemical dual-mode test strip for detecting OA and a preparation method thereof, and belongs to the technical field of biological detection. The test paper is composed of a bifunctional signal probe, a chromatography matrix and an integrated detection module. Cellulose paper is adopted to replace a traditional nitrocellulose membrane to serve as a chromatography substrate, and a screen-printed electrode is integrated in a detection area, so that colorimetric and electrochemical dual-mode signal output can be synchronously triggered by loading a single sample. Wherein the hollow MOF structure realizes physical isolation of two signal carriers AuNPs and Fc-NH2 through a space confinement effect, and signal cross interference is effectively eliminated; the colorimetric module supports on-site rapid screening, and the electrochemical module realizes high-sensitivity quantitative detection. According to the method, the reliability of a detection result is remarkably improved through mutual verification of double signals, the functions of visual interpretation and accurate analysis are achieved, operation is easy and convenient, additional auxiliary steps are not needed, and the method is particularly suitable for rapid detection of trace target objects in complex samples.

The invention belongs to the field of food safety detection, and particularly relates to a method for rapidly detecting preservatives in condiments based on a molecularly imprinted sensing material. Qualitative / quantitative detection of preservatives in seasonings is realized based on the sensor, and the sensor is formed by sequentially modifying a microstructure layer containing mesoporous carbon spheres to promote optimal imprinting polymerization sites and an imprinting polymerization site layer on the surface of a screen-printed electrode. According to the method, the preservative molecules in the condiment can be specifically recognized and enriched, complex pretreatment steps and detection processes of a large instrument are eliminated, and quick detection of the preservative in the condiment is realized. According to the invention, the detection technology can be optimized from three aspects of shortening the detection time, reducing the reagent dosage and being exquisite and portable, and a novel method suitable for field detection is provided for detection of the preservatives in the condiments.