52 results about "Screen printed electrode" patented technology

A wearable diagnostic electrocardiogram (ECG) garment is disclosed herein. The wearable diagnostic ECG garment comprises a garment body, screen-printed electrodes positioned on the body, and screen-printed wires positioned in the garment body, each of the screen-printed wires connected from a central connector module to a screen-printed electrode.

The utility model discloses a metformin hydrochloride electrochemical analysis device which comprises a shell, and one side of the shell is provided with an electric sensor interface; the power supply unit is arranged in the shell and is used for generating polarization voltage; the silk-screen printing electrode electric sensor is electrically connected with the power supply unit and comprises a flexible electrode base body, a working electrode, a counter electrode, a reference electrode, an insulating layer, a wiring terminal and an electrode connecting wire, and the working electrode, the counter electrode, the reference electrode, the insulating layer, the wiring terminal and the electrode connecting wire are arranged on the flexible electrode base body; the surface of the working electrode is modified with an imprinted polymer with selective recognition on metformin hydrochloride; the signal processing unit is arranged in the shell and comprises a shunt resistor, an operational amplifier, a signal converter, a data storage chip and a microprocessor which are electrically connected in sequence; the display screen is electrically connected with the microprocessor and is used for displaying the converted concentration value; the method has the advantages that the metformin hydrochloride can be rapidly and accurately detected, and the use is relatively convenient.

The invention belongs to the technical field of electrochemical analysis and detection, and particularly relates to an electrochemical immunosensor for detecting adiponectin as well as a preparation method and application of the electrochemical immunosensor. The preparation method comprises the following steps: (1) connecting a screen-printed electrode SPCE to an electrochemical workstation by using a 0.01 M PBS (Phosphate Buffer Solution) with the pH value of 7.4, performing-0.2 V-1. 2V cyclic voltammetry scanning, and activating for 10 circles under 50mV / S; and (2) by taking 0.01-1M H2SO4 as a base solution, connecting the screen-printed electrode SPCE to an electrochemical workstation, carrying out electro-polymerization on poly-o-phenylenediamine in a 50-100mM o-phenylenediamine and NH2-UiO-66 solution to form a poly-o-phenylenediamine and NH2-UiO-66 composite modified electrode, washing with deionized water, and drying at room temperature. (3) soaking the modified electrode in a glutaraldehyde solution for 10-70 minutes, cleaning with deionized water, drying at room temperature, adding 10-100 [mu] L of ADPN antibody solution and 100-600 [mu] g / mL of ADPN antibody solution, and incubating; and (4) washing with ionized water, drying at room temperature, preventing non-specific binding sites at 37 DEG C for 0.5-1.5 hours by using 3-10 microliters of 0.8-1.2 wt% casein solution, and washing the screen-printed electrode again to obtain the electrochemical immunosensor.

The invention relates to a surface modified Fe / Cu-Co3O4 catalyst for nitrite detection and a preparation method thereof, a sensor and application, and the preparation method comprises the following steps: dissolving a cobalt source, an iron source, a copper source, urea and NH4F in deionized water, carrying out a hydrothermal reaction, washing, drying and calcining to obtain a Fe and Cu co-doped Co3O4 nanotube; and pre-hydrolyzing by using a KH792 silane coupling agent ethanol-water solution, and reacting with nanotubes to prepare the surface modified catalyst. The sensor takes carbon cloth or a screen-printed electrode subjected to ultrasonic cleaning and plasma treatment as a substrate, catalyst ink is coated to form a catalytic layer, and the catalytic layer, a platinum counter electrode and a silver / silver chloride reference electrode form a three-electrode system. Through molecular modification and material design, the interfacial force between the catalyst and nitrite is improved, the electrode stability is enhanced, and the catalyst is applied to a disposable printed electrode and portable detection equipment, so that high-sensitivity, stable and portable detection of nitrite is realized.

The application provides an electrochemical sensing ELISA reagent card, comprising a microfluidic channel body and an electrochemical sensor body which are bonded together, wherein: a plurality of "annular" liquid flow channels are arranged on the microfluidic channel body, each "annular" liquid flow channel is formed by a working electrode covering flow channel and a counter electrode and a reference electrode covering flow channel in series, and the "annular" liquid flow channels are communicated through a common flow channel; the electrochemical sensor body comprises a flat plate substrate and a plurality of screen-printed electrodes which are printed in parallel on the flat plate substrate, and each screen-printed electrode comprises a working electrode, a counter electrode and a reference electrode. The electrochemical sensing ELISA reagent card combines an electrochemical biosensor and a microfluidic chip, and solves the technical problems of large sample / reagent consumption, single detection index and liquid volume influence on absorbance measurement of a traditional ELISA reagent kit.

The invention relates to the technical field of electrochemical sensing, in particular to a multi-channel electrochemical aptamer sensor and a preparation method and application thereof.The multi-channel electrochemical aptamer sensor adopts a commercial silk-screen printing electrode and is combined with a polymer substrate material for amplifying electrochemical signals, and the electrochemical aptamer sensor is prepared. According to the present invention, the corresponding aptamer is fixed by using the gold nanoparticle through the chemical bond so as to specifically detect the three hemorrhage conversion biomarkers after acute cerebral infarction, such as human matrix metalloproteinase (MMP-9), fibrinogen (FN) and plasminogen activation inhibitor (PAI-1); wherein the FN and the PAI-1 are detected through an electrochemical aptamer sensor method for the first time, and simultaneous detection of the three biomarkers is realized through integration with commercial multi-channel micro electrochemical equipment.

The invention is applicable to the technical field of biological detection, and provides a method for detecting staphylococcus aureus based on combination of a screen-printed electrode and CRISPR / Cas13a, a probe containing a DNA / RNA hybrid chain is combined with a magnetic bead to form a simple composite structure, when the Cas13a recognizes 16sRNA of S. aureus, trans-cleavage activity can be immediately activated, the probe chain is rapidly cut off, the cut-off amount depends on the activated amount, and when the Cas13a recognizes 16sRNA of S. aureus, the Cas13a can be used for detecting staphylococcus aureus. And after transient magnetic separation, a result is observed on the screen-printed electrode. The method has ultrahigh specificity: the CRISPR / crRNA sequence ensures that staphylococcus aureus and other microorganisms can be accurately distinguished; the trans-cutting of Cas13a provides chemical amplification, and the magnetic separation purification greatly reduces background signals caused by non-specific adsorption, so that the signal-to-noise ratio and the sensitivity are remarkably improved.

The invention provides a method for rapidly detecting pesticide residues in fruits and vegetables, and belongs to the field of pesticide analysis and detection. The method comprises the following steps: mixing a copper nanoparticle / reduced graphene oxide / chitosan composite material, acetylcholin esterase, choline oxidase and a cross-linking agent glutaraldehyde solution for reaction to obtain a double-enzyme functionalized nano composite material; dispensing a chitosan solution on the surface of a working electrode of a screen-printed electrode, drying to form a film, and covalently modifying the surface of the electrode with the bienzyme functionalized nano composite material through a cross-linking agent glutaraldehyde to obtain a sensing electrode; according to the method, acetylcholine is taken as a substrate, a sensing electrode is used, a chronoamperometry is adopted, inhibition ratios of pesticide standard substance solutions with different known concentrations to electrode response current are detected, and a standard curve between the pesticide concentration and the current inhibition ratio is established; and calculating the pesticide residue concentration in the fruit and vegetable sample to be detected according to the current inhibition ratio of the liquid to be detected and the standard curve. Therefore, the sensitivity and accuracy of pesticide residue detection in fruits and vegetables are improved.

The invention discloses a method for detecting the concentration of acetic acid in an anaerobic fermentation system by using a bioelectrochemical sensor, acetic acid is detected by using the bioelectrochemical sensor, the bioelectrochemical sensor comprises a screen-printed electrode, and the working surface of the screen-printed electrode is modified with reduced graphene oxide and polymethylene blue. The working surface of the screen printing electrode is connected with acetokinase, pyruvate kinase and lactic dehydrogenase through a cross-linking agent. According to the method, the adopted sensor has better conductivity, higher response speed and higher current intensity, when the sensor is used for detecting the concentration of acetic acid in a fermentation system, the sensor has the advantages of being easy to operate, low in sample consumption, low in price, high in accurate measurement precision, high in detection speed and the like, and rapid detection of the concentration of acetic acid can be achieved; the acetic acid concentration in the anaerobic fermentation process in different periods can be timely and accurately obtained, effective data can be provided for anaerobic fermentation analysis, and the method has important significance for monitoring the process stability of anaerobic fermentation.

The invention provides a molecular classifier for detecting gastric cancer miRNA. A preparation method of the molecular classifier comprises the following steps: depositing a PABA modification layer on the surface of a screen-printed electrode; activating carboxyl on the surface of the para aminobenzoic acid modification layer by adopting an EDC / NHS composite system; carrying out amination modification on an MXene material, and coating the surface of the activated para aminobenzoic acid modification layer with the modified MXene material to form an MXene functional layer; depositing gold nanoparticles on the surface of the MXene functional layer to obtain an electrochemical activity sensing interface; a signal reporter molecule is combined with an aptamer probe, the signal reporter molecule is a DNA tetrahedron-methylene blue complex, and the combined complex is dropwise added on the surface of an electrochemical activity sensing interface, so that the aptamer probe is combined on the surface of the electrochemical activity sensing interface. The molecular classifier can be used for preparing gastric cancer miRNA detection products, and the problems that in the prior art, a classifier is high in complexity and high in signal leakage risk are solved.

The invention discloses a high-sensitivity Tau-441 electrochemical immunosensor based on a nano material synergistic amplification effect as well as a preparation method and application of the high-sensitivity Tau-441 electrochemical immunosensor. According to the sensor, a silk-screen printing electrode (SPE) is used as a substrate, a PEDOT: PSS / MWCNTs-COOH nanocomposite modified working electrode is used as a sensing platform, and electron transfer is promoted by using the large specific surface area and excellent conductivity of the PEDOT: PSS / MWCNTs-COOH nanocomposite modified working electrode. Meanwhile, gold-modified magnetic nanoparticles (Fe3O4-coated AuNPs) are prepared as a signal probe, and the magnetic separation and enrichment function of Fe3O4 and the biocompatibility and catalytic activity of the gold nanoparticles are combined. The sandwich immune complex is constructed by adopting a homogeneous reaction mode, so that the mass transfer resistance is effectively reduced, and the detection time is shortened. The sensor has an extremely low detection limit (19.68 fgmL <-1 >) and a wide linear range on an Alzheimer's disease marker Tau-441, is high in specificity and good in stability, and is suitable for early-stage rapid screening of human plasma samples.

The invention discloses a method for rapidly detecting inorganic arsenic in aquatic animals. The method comprises the following steps: pre-treating an aquatic animal sample to be detected to obtain a sample solution to be detected; the method comprises the following steps: taking a silk-screen printing electrode as a working electrode, electrifying a to-be-detected sample solution by adopting anodic stripping voltammetry to collect an electrical response parameter in the electrifying process, and further obtaining the content of inorganic arsenic according to the electrical response parameter. According to the method, the screen-printed electrode is adopted to replace a traditional glassy carbon electrode or gold electrode, polishing is not needed, operation is easy, throwing is achieved when the electrode is used, the price is low, batch production is easy, and the performance is stable, and the electrochemical anodic stripping voltammetry is applied to detection of inorganic arsenic in aquatic animals for the first time; the shape of the working electrode and an ink carbon-based material in the working electrode are optimized, and the quantitation limit of the finally measured sample is as low as 0.1 mg / kg; the method is simple, rapid and efficient, and is suitable for large-scale production and application on a base layer.

The application discloses a one-step monoclonal antibody drug electrochemical detection biosensor and a preparation method and application thereof. The application designs and screens an affinity specific DNA aptamer hairpin probe and uses the same as a specific capture unit, then uses a gold-sulfur bond to fix the DNA aptamer hairpin probe on a screen-printed electrode surface, meanwhile, uses a self-assembly interaction between a metal and a mercapto group to fix a blocking agent introduced on the screen-printed electrode surface, and blocks the excess electrode surface reaction sites, so that the one-step monoclonal antibody drug electrochemical detection biosensor is obtained. The one-step monoclonal antibody drug electrochemical detection biosensor has the advantages of simple operation, high sensitivity, less non-specific adsorption, high capture efficiency, stable physical and chemical properties, strong specificity and good selectivity, and can be used for capturing antibody drugs in complex biological samples.

This invention belongs to the field of rapid detection technology, specifically relating to a rapid, real-time detection method for influenza A based on electrochemical biosensors. This invention provides an aptamer that specifically recognizes the influenza A virus, and uses a pairing affinity reagent to enrich the aptamer onto magnetic beads to prepare a probe that specifically recognizes the influenza A virus. Then, using this probe, MB solution, and screen-printed electrodes, an electrochemical biosensor that specifically recognizes the influenza A virus is fabricated. Qualitative or quantitative detection of influenza A virus using the electrochemical biosensor of this invention eliminates the need for complex pretreatment and viral nucleic acid amplification, greatly simplifying the operation. The results are stable and reliable, making it suitable for real-time detection applications.

The invention relates to a method for producing an electrode-coated cell layer (100) for an electrochemical cell stack, in particular a fuel cell stack or an electrolysis cell stack, and at least one catalyst-containing electrode (120, 130), in particular an anode electrode (120) and / or a cathode electrode (130), for the cell stack, is applied to a cell stack substrate (110), wherein an electrode coating material is printed onto a large-area outer surface of the cell stack substrate (110) by means of a screen printing process as a screen-printed electrode (120, 130) patterned in two length dimensions and structured in its thickness dimension (D).

The invention relates to a molecularly imprinted field effect transistor sensor and a preparation method and application thereof, and a screen-printed electrode assembly comprises a substrate, and a carbon working electrode and a silver / silver chloride reference electrode which are oppositely arranged on the substrate; a gold nanoparticle layer, a polydopamine connecting layer, a beta-cyclodextrin main body layer and an ionic liquid doped poly-o-phenylenediamine molecular imprinting layer are sequentially overlaid on the carbon working electrode. Compared with the prior art, the invention provides a molecular imprinting strategy based on beta-cyclodextrin orientation, accurate pre-orientation of a perfluoroalkyl substance (PFAS) template molecule is realized by using a hydrophobic cavity of beta-cyclodextrin through a host-guest inclusion effect, the uniformity and specificity of an imprinting cavity are effectively improved, and the accuracy and the specificity of the imprinting cavity are improved. The core problem that recognition sites are disordered due to random distribution of templates in a traditional molecular imprinting technology is solved.

The application discloses a kind of based on carboxylated multi-walled carbon nanotube modified silk screen printing electrode preparation azide ion electrochemical sensor.The application uses carboxylated multi-walled carbon nanotube as modification material, acetic acid solvent, and electrode modification liquid of 2mg / mL is prepared, 2 μL is dropped on the working carbon electrode of silk screen printing electrode, and N3 ‑ Electrochemical sensor. The concentration of azide ion in the solution is detected by electrochemical analysis method, and the sensor has the advantages of easy preparation, portability, sensitivity, low price and small required detection amount.

The invention belongs to the field of food safety detection, and particularly relates to a method for rapidly detecting preservatives in condiments based on a molecularly imprinted sensing material. Qualitative / quantitative detection of preservatives in seasonings is realized based on the sensor, and the sensor is formed by sequentially modifying a microstructure layer containing mesoporous carbon spheres to promote optimal imprinting polymerization sites and an imprinting polymerization site layer on the surface of a screen-printed electrode. According to the method, the preservative molecules in the condiment can be specifically recognized and enriched, complex pretreatment steps and detection processes of a large instrument are eliminated, and quick detection of the preservative in the condiment is realized. According to the invention, the detection technology can be optimized from three aspects of shortening the detection time, reducing the reagent dosage and being exquisite and portable, and a novel method suitable for field detection is provided for detection of the preservatives in the condiments.

The invention provides a cobalt and nickel ion detection method which comprises the following steps: adding a buffer solution and dimethylglyoxime into a water sample for reaction, and adding lead ions and copper ions to obtain a water sample to be detected; the method comprises the following steps: putting a water sample to be detected into a stirring container, inserting a screen-printed electrode into the stirring container, adjusting parameters, and detecting cobalt and nickel ions in the water sample to be detected; wherein the adding sequence of the buffer solution and the dimethylglyoxime is not limited. According to the detection method disclosed by the invention, the regulation and control effect of an ammonia water buffer solution and the high selectivity of dimethylglyoxime are fully utilized, a screen-printed electrode is introduced, and a solution containing lead and copper ions is adopted for coating, so that an ingenious separation means and a masking mechanism are obtained, and effective distinguishing and accurate determination of cobalt and nickel ions are realized.

The invention relates to the technical field of biological detection, and provides an integrated electrochemical detection device for stomach cancer postoperative MRD auxiliary detection as well as a preparation method and application of the integrated electrochemical detection device. The core of the device is that a functional nanofiber sensing interface layer and a preset freeze-drying reagent layer are sequentially constructed on a working area of a screen printing electrode (SPE). Wherein the sensing interface layer fixes an anti-CD63 capture antibody through an electrostatic spinning polyacrylic acid (PAA) nanofiber membrane, and is used for efficiently enriching plasma exosomes; and the freeze-drying reagent layer comprises a detection antibody of a targeted gastric cancer marker Claudin18.2, a streptavidin-horse radish peroxidase signal reporting system and a freeze-drying protective agent. According to the invention, the unification of high precision of detection and extremely simplified operation is realized through a unique'mask protection 'process and a'reagent freeze-drying integration' technology. The device has the outstanding advantages of high detection sensitivity, good specificity, convenience in operation, good accuracy and the like.

This invention provides a method and apparatus for perioperative fibrinogen detection, comprising the following steps: S1, modifying the working electrode surface of a screen-printed electrode with antibody-functionalized self-supporting graphene aerogel material; S2, adding the serum sample to be tested to the modified working electrode surface; S3, adding horseradish peroxidase-labeled fibrinogen detection antibody to the modified working electrode surface; S4, adding a matrix-type TMB solution containing H2O2 to the modified working electrode surface; S5, collecting the reduction current of electrochemically active substances on the electrode surface using a chronoamperometry method; S6, calculating the fibrinogen concentration based on the reduction current value and a standard curve. This invention solves the technical problems of low electron transport efficiency and detection sensitivity, structural instability, poor biocompatibility, and difficulty in enriching electroactive substances when using graphene-modified electrodes.

The invention discloses a method for detecting ovalbumin through an electrochemical bionic small intestine micro-fluidic chip sensor and application, and belongs to the technical field of food safety detection. A micro-intestinal chip is designed by simulating the structure of the small intestine of a human body, an anti-IL-6 antibody is integrated on a screen-printed electrode to serve as a specific recognition element, a sensor capable of being used for specifically recognizing cell secretion IL-6 is constructed, and effective and indirect measurement of OVA is achieved. An OVA standard substance is used for testing the bionic small intestine sensor, a good linear relation exists between the resistance value detected by an electrochemical means and the OVA concentration, the linear detection range is 0.2 pg / mL to 4 pg / mL, the detection limit is 0.161 pg / mL, and it is proved that the bionic small intestine sensing interface is feasible and the sensor can conduct quantitative sensitive detection on the allergen protein.

The invention discloses an electrochemical sensor based on an N-MLG coated Ni-Mn LDH / Cys composite material and a preparation method and application thereof, and belongs to the technical field of electrochemical sensors. The sensor adopts a three-electrode system, takes an N-MLG coated Ni-Mn LDH / Cys composite material modified screen-printed electrode (SPE) as a working electrode, takes Ag / AgCl as a reference electrode and takes a carbon electrode as a counter electrode, and detects copper ions by utilizing square wave anodic stripping voltammetry. By means of the high conductivity of N-MLG, the large specific surface area of Ni-Mn LDH and the specific chelating capacity of cysteine, a current signal is amplified, and the sensitivity is remarkably improved. The electrochemical sensor constructed by the invention has the outstanding advantages of low detection limit, wide linear range, high selectivity and the like in the aspect of sweat copper ion detection, is simple and convenient to operate, and has huge potential in personalized medical treatment and clinical diagnosis.

The invention relates to a method for detecting GP73 by constructing a dual-signal ratio type aptamer sensor based on a carbon-based nanocomposite, which comprises the following steps: modifying nanogold on the surface of a screen-printed electrode, fixing a GP73apt2-NSrGO-Cu2O reference probe on the surface of the electrode through an Au-SH bond, and oxidizing Cu (I) into Cu (II) to provide a reference signal I (Cu2O); the preparation method comprises the following steps: preparing an NSrGO-H-MoS2-GP73apt1 signal probe; therefore, the sandwich type electrochemical aptamer sensor is constructed. Fe (II) in Hemin in the NSrGO-H-MoS2 nano composite material is oxidized into Fe (III) to obtain another electrochemical signal I (hemin), and a function relationship between I (hemin) / I (Cu2O) and the concentration of GP73 is constructed, so that accurate detection of GP73 is realized.

The utility model relates to the technical field of silk screen printing electrode, concretely to a silk screen printing electrode with isolation structure, including base plate, the positive surface fixedly connected with working electrode of base plate, one side fixedly connected with the isolation board of working electrode, the positive surface of base plate is opened groove, the isolation board is fixedly connected through the groove in the positive surface of base plate, the top of isolation board is opened and is limited to groove, the inside swing joint of limit groove has expansion block, the outside of isolation board is provided with the protective layer, one side fixedly connected with reference electrode of working electrode. This silk screen printing electrode with isolation structure, through install the isolation board, can protect working electrode after solution drop coating in working electrode surface, avoid solution to scatter, cause short circuit to occur in three electrodes, simultaneously if the solution of drop is too much, can through limit groove, extract the expansion block in its inside, increase the area of isolation, and then improve the practicality of the device.

The invention provides an electrochemical sensor based on an MOF-on-MOF and MWCNTs-COOH synergistic signal amplification effect as well as a preparation method and application of the electrochemical sensor based on the MOF-on-MOF and MWCNTs-COOH synergistic signal amplification effect. The sensor comprises a silk-screen printing electrode, MWCNTs-COOH modified on the surface of the silk-screen printing electrode, and flower-shaped MIL-88B-NH2-coated UiO-66-NH2, and the MWCNTs-COOH is modified on the surface of the silk-screen printing electrode. Wherein the MIL-88B-NH2 (at) UiO-66-NH2 is synthesized by carrying out in-situ growth on the MIL-88B-NH2 on the surface of a UiO-66-NH2 crystal, so as to obtain the MIL-88B-NH2 (at) UiO-66-NH2 crystal. The sensor can be used for detecting Pb < 2 + >, Cu < 2 + > and / or Hg < 2 + > in a water sample, an MIL-88B-NH2 (at) UiO-66-NH2 / MWCNTs-COOH / SPCE modified electrode is used as a working electrode, an Ag / AgCl electrode is used as a reference electrode, a carbon electrode is used as an auxiliary electrode, differential pulse anodic stripping voltammetry (DPASV) is adopted for detection, a redox peak of heavy metal ions (HMIs) is used as an index signal, and no extra signal label needs to be introduced. The linear range of the sensor is 0.10-25 [mu] M, and the detection limits of the sensor are respectively as low as 5.55 nM, 4.16 nM and 6.38 nM. The sensor prepared by the invention shows excellent selectivity, reproducibility and stability on HMIs in river water and tap water.

The application discloses a kind of Co nanoparticle loaded carbon nanotube magnetic functional materials (Co@CNTs) preparation. Due to the synergistic effect of Co nanoparticle and carbon tube, the material shows excellent electrocatalytic, conductivity, fast electron transfer ability. Combined with the design of magnetic base and screen printed electrode (SPE), a kind of renewable magnetic screen printed electrode is developed. Among them, the presence of Co@CNTs greatly improves the electron transfer dynamics of hydrazine hydrate and tartrazine on the surface of SPE. The linear range of Co@CNTs / SPCE for hydrazine hydrate and tartrazine detection is 0.1~50.0 mM and 0.04~30.0 mM, and the detection limit is 50 nM and 12 nM respectively. Since Co@CNTs is attached to the surface of SPE by magnet control, and is easy to separate from the electrode surface by magnet, the sensor structure is simple, renewable and low cost.

The application relates to the technical field of tumor detection, in particular to an AuNPs@Co-LDH@ZIF-67 composite and a preparation method and application thereof; the biosensor comprises a working electrode and an anti-EGFR antibody-AuNPs@Co-LDH@ZIF-67 bioconjugate, the working electrode comprises a screen-printed electrode (SPCE), and the detection end surface of the screen-printed electrode (SPCE) is sequentially connected with chitosan (Chi) and AuNPs@Co-LDH@ZIF-67, namely the biosensor is AuNPs@Co-LDH@ZIF-67 / Chi / SPCE. The biosensor has good oxidase simulation activity, the chitosan is combined to increase the loading of the antibody, the detection performance of the electrode is greatly improved, and the tumor cells can be efficiently detected.

This invention discloses an electrode for detecting cTnI content and its preparation method, comprising a screen-printed electrode and a biorecognition layer; the screen-printed electrode includes a working electrode; the biorecognition layer is modified on the surface of the working electrode; the biorecognition layer includes conductive graphene nanomaterials for immobilizing cTnI capture antibodies; the conductive graphene nanomaterials are modified on the surface of the working electrode by a binder; the conductive graphene nanomaterials include carboxylated graphene oxide. This invention can rapidly and accurately detect different concentrations of myocardial markers. The prepared electrochemical immunosensor is simple and effective, not only effectively realizing the detection of different concentrations of myocardial markers, but also providing a model for subsequent research on constructing electrochemical immunosensors for other markers due to the simplicity of the substrate material.

The invention relates to the field of heavy metal electrochemical detection, in particular to a scheme for simplifying the structure of a heavy metal electrochemical testing instrument. The enrichment of heavy metal ions to be detected on the surface of the screen-printed electrode is promoted by regulating and controlling the vibration of the interface part of the heavy metal detector and the screen-printed electrode. According to the scheme, the detection effects of the heavy metal detector under the vibration condition and the vibration-free condition are compared, it is found that the promotion effect similar to that of an external stirring device can be achieved by adjusting and controlling vibration of the detection port of the heavy metal detector, the actual requirement is met, the structure of a test instrument is simplified, the instrument cost is effectively reduced, and operation is more concise.