This invention provides a method for chiral recognition of
phenylalanine, comprising the following steps: (1) preparing a ReSe2 film using NH4ReO4, NaCl, and
selenium powder; (2) preparing gold nanoparticles using HAuCl4, NaBH4, and CTAB and AA at different concentration ratios, and inducing the formation of gold nanoparticles with different chiralities by adding
cysteine solutions; (3) dropping the prepared gold
nanoparticle colloids with different chiralities onto the above ReSe2 film, and after complete
air drying, dropping in a concentration range of 10 ‑3 -10 ‑7 (4) Using a
fully automated laser confocal micro Raman spectrometer, Au-ReSe2 substrate materials loaded with
phenylalanine of different chiralities were tested. This invention can achieve sensitive detection at a specific
deflection angle, and at the same time, through the interaction between the
enantiomer and the specific chiral substance, it exhibits a certain difference, which not only enhances the Raman
signal, but also achieves good detection range for low concentrations of Phe (10 mol / L). ‑3 -10 ‑7 It has the advantages of strong molecular
signal and wide detection range, with chiral recognition capability of mol / L.