30results about How to "Achieving Sensitive Detection" patented technology

The invention discloses an electrochemical luminescence immunosensor with low trigger potential and a construction method and application thereof, the sensor comprises a three-electrode system of an immune electrode, a reference electrode and a counter electrode, and when the electrochemical luminescence immunosensor is used, a detection solution comprises luminol and hydrogen peroxide; the immunoelectrode is an SNF modified electrode confined with metal palladium nanoparticles and nitrogen-doped graphene quantum dots, immunoglobulin A of immunospheres is covalently immobilized, then a primary antibody of a directionally immobilized tumor marker is constructed, and non-specific sites are closed, so that the immunoelectrode is obtained. The independent nano channel of the SNF film effectively avoids agglomeration of a confinement material, and the in-situ deposition mode is efficient and rapid; the good biocompatibility of the SNF provides a high-quality platform for the preparation of an immunosensing interface. The prepared immunosensor has the advantages of fast response speed, high ECL signal and good selective anti-interference capability, and has potential application prospects in tumor marker analysis and detection.

The invention discloses a bimodal polymyxin drug-resistant gene MCR-1 detection platform, relates to the field of food safety and analytical chemistry, and in particular relates to an on-site rapid detection method of the polymyxin drug-resistant gene MCR-1 in a food sample, primer groups aiming at the polymyxin drug-resistant gene MCR-1 are respectively as shown in SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4 and SEQ ID NO.5, and a sixth primer with the 5'end labeled by biotin; the CRISPR / dCas9 system comprises dCas9 protein and sgRNA, and the sequence of the sgRNA is marked as SEQ ID NO.7; the sequence of the gold-platinum nanoprobe is marked as SEQ ID NO.8; the LAMP technology is adopted to amplify the polymyxin drug-resistant gene MCR-1 in the sample to be detected, an LAMP product contains multiple sections of reverse circulation repetitive sequences, and each double strand can be combined with more gold-platinum nanoparticles, so that sensitive detection of the food-borne pathogenic bacteria containing the polymyxin drug-resistant gene MCR-1 in the food sample is realized, the sensitivity is relatively high, the detection time is short, the detection time is short, and the cost is low. The operation is simple, the stability is high, and the whole detection process can be completed in a short time.

The invention discloses a preparation method and application of a sensor array based on luminous carbon dots, and relates to the technical field of sensors, the preparation method comprises the following steps: preparing a carbon source which comprises a precursor containing a targeting functional group or a biomolecule derived carbon source, preparing the luminous carbon dots through a chemical synthesis or physical method, modifying the surfaces of the carbon dots, and preparing the sensor array based on the luminous carbon dots. A core-shell structure is added on the surface of a carbon dot by using an in-situ growth method or a layer-by-layer self-assembly method, the carbon dot with the added core-shell structure is fixed on a substrate of a sensor array, and a performance test is carried out. By synthesizing the carbon dots with a targeted recognition function, the selectivity and the sensitivity of the sensor to a target substance can be remarkably improved, and in the synthesis process, the carbon dots can generate specific interaction with the target substance by adjusting factors such as the structure, the shape, the size and the surface chemical property of the carbon dots; therefore, accurate identification of the target substance is realized, and detection of the marker is realized.

The invention discloses a bionic flow field sensor array inspired by a mosquito antenna variable fulcrum cantilever structure. The bionic flow field sensor array comprises a central hard cantilever beam and bionic variable fulcrum cantilever beam arrays sequentially arranged on the two sides of the central hard cantilever beam. The bionic variable fulcrum cantilever beam is connected to the central hard cantilever beam in a rigid-flexible coupling drawing and inserting manner; each bionic variable fulcrum cantilever beam comprises a variable stiffness flexible elastic substrate and a conductive circuit; the conductive circuit is distributed on the surface of the variable-rigidity flexible elastic substrate and covers the whole variable-rigidity area; the bionic variable-fulcrum cantilever beam array simulates a rigid-flexible coupling structure of antennae of mosquitoes; and the rigidity of the bionic variable-fulcrum cantilever beam array is gradually reduced from the bottom end to the top end, so that deformation is allowed to be triggered under the action of airflow. The limitation of an existing flow field sensor on the detection range is solved, and the flow velocity detection range is expanded by reducing the lower detection limit and improving the upper detection limit.

The present invention provides an electrochemiluminescent aptamer sensor for detecting synthetic cathinone MDPV, and a preparation method and application thereof, which belong to the field of electrochemiluminescence detection. The electrochemiluminescent aptamer sensor is formed by loading an aptamer SF on the surface of a glassy carbon electrode modified by a composite material In‑MOF@Sn‑TCPP; the composite material In‑MOF@Sn‑TCPP is In‑MOF coated with Sn‑TCPP (5,10,15,20‑tetrakis(4‑carboxyphenyl)tinporphyrin). The detection method is to use a SF / In‑MOF@Sn‑TCPP / GCE modified electrode as a working electrode, an Ag / AgCl electrode as a reference electrode, and a platinum electrode as an auxiliary electrode to form a traditional three-electrode system. The preparation method of the modified electrode in the present invention is simple, and the sensitivity to MDPV detection is high, the selectivity is good, and the linear range is wide.

The invention relates to the technical field of environment monitoring, in particular to a multi-dimensional track infrared temperature sensing ozone detection and positioning alarm cue ball which comprises an ozone detection cue ball shell made of a corrosion-resistant light material, and a plurality of ozone capturing flying insects are arranged in the ozone detection cue ball shell. An infrared ray temperature sensor, a detection liquid release mechanism and a detection liquid storage container are mounted on the ozone capture flying insect; the infrared ray temperature sensor is used for continuously monitoring the temperature change of each point on the track; the cue ball emits ozone in multiple directions of the space to capture flying insects, comprehensive monitoring of the ozone concentration in the three-dimensional space can be achieved, and compared with a traditional single-point or fixed-direction detection mode, the detection comprehensiveness and accuracy are greatly improved; and an infrared ray temperature sensing technology is utilized, and the direction of an ozone generation source can be accurately tracked and positioned through judgment of a temperature difference value on a trajectory, so that powerful support is provided for investigation and treatment of a pollution source.

The present invention belongs to the field of electrochemiluminescence detection, and relates to an electrochemiluminescence aptamer sensor for specific detection of enrofloxacin, as well as a preparation method and application. The sensor is formed by loading an enrofloxacin aptamer on the surface of a glassy carbon electrode modified with a NaYF4-PEI-PTCA nanocomposite material, wherein the NaYF4-PEI-PTCA nanocomposite material is a product of upconversion nanoparticles and perylene tetracarboxylic acid bound by an amide bond; the nucleotide sequence of the enrofloxacin aptamer is apt:5'-CCC ATC AGG GGG CTA GGC TAA CAC GGT TCG GCT CTC TGA GCC CGG GTT ATT TCA GGG GGA-3'. The sensor of the present invention can realize sensitive detection of enrofloxacin, and the minimum detection limit is 3.0×10 ‑15 mol / L.

The present invention discloses a method and sensor for detecting L-cystine. A poly(disulfide-dipropanesulfonic acid) film is assembled on the surface of a gold film layer of a gold electrode as a gate extending from a field effect transistor. The field effect transistor in-situ signal amplification function is used to realize sensitive detection of L-cystine. The polyanion of the poly(disulfide-dipropanesulfonic acid) film adsorbs and binds the positively charged target L-cystine through electrostatic action to form a double electric layer structure to generate a membrane potential for identifying monovalent organic ammonium ions. The sensor has a good Nernst response relationship for L-cystine, and the linear range is 5.0×10 ‑6 —1.0×10 ‑3 mol / L, the response sensitivity was 58.25±1.5mV / ‑pc (25°C), and the detection limit was 2.69×10 ‑6 mol / L, which can be used for the rapid and sensitive detection of L-cystine in pig serum samples, and has potential application prospects in the fields of healthy breeding and life sciences.

The application discloses a kind of SPWM control's wireless charging system metal foreign matter detection device and method, including wireless power transmitting unit, wireless power receiving unit, metal foreign matter detection unit and control unit, the wireless power transmitting unit includes the SPWM modulation circuit, inverter circuit and transmitting coil connected in sequence, the metal foreign matter detection unit includes detection coil group, detection module connected in sequence, the detection coil group is laid on transmitting coil;The detection module includes parallel resonance circuit, high-pass filter circuit and differential amplifier circuit connected in sequence;The detection coil of the application can improve the detection sensitivity of small metal foreign matter.

The invention discloses a cyclodextrin bionic organic iridium magnetic catalyst as well as a preparation method and application thereof, and belongs to the field of catalysts and application. The catalyst is prepared from three parts, namely magnetic nanoparticles, beta-cyclodextrin and an adamantane functionalized 2-pyridine benzamide type organic iridium catalyst, the magnetic nanoparticles are surface carboxylated magnetic nanoparticles, are activated by N-hydroxysuccinimide and then are connected with aminated beta-cyclodextrin through amido bonds; the beta-cyclodextrin is connected with adamantane in the adamantane functionalized 2-pyridine benzamide type organic iridium catalyst structure through a host-guest coupling effect. The invention further discloses application of the combination of the catalyst and a rhodamine 110 type quinone reduction regulation fluorescent probe in coenzyme NADH (Nicotinamide Adenine Dinucleotide Hormone) detection. The cyclodextrin bionic organic iridium magnetic catalyst MBs (at) CD (at) Ir prepared by the invention has excellent biocompatibility, and the constructed NADH fluorescent probe has the advantages of high sensitivity, good selectivity and easiness in recovery of the catalyst.

The invention belongs to the technical field of biology, and particularly relates to a promoter responding to pentanediamine, a pentanediamine biosensor and application of the pentanediamine biosensor. The original PpyrF promoter can respond to the concentration of pentamethylene diamine and regulate and control the expression of report protein GFP, the promoter PpyrF-D40 or PpyrF-F5 is obtained by truncation or mutation optimization of the promoter PpyrF, and the dynamic range and sensitivity of the pentamethylene diamine biosensor are improved. The pentane diamine biosensor further constructed on the basis of the promoter PpyrF-D40 or PpyrF-F5 has no responsiveness to a pentane diamine synthesis precursor lysine, so that the biosensor has good specificity when being applied to screening of pentane diamine production strains. The fluorescence intensity value of the pentane diamine biosensor obtained through screening shows an excellent linear response relationship with the concentration of pentane diamine, and the pentane diamine biosensor can be used for efficiently breeding a pentane diamine high-yield strain by being combined with a high-throughput screening system and the like, and has the advantages of high detection efficiency, high sensitivity and the like.

A novel organic photoelectrochemical transistor aptamer (OPECT) biosensor is developed, photoelectrochemical analysis and an organic electrochemical transistor are combined, AgI-TiO2 (AgI-TNs) is used as a photoactive material, target specific DNA chain reaction hybridization is used as a signal amplifier, and the sensor is used for detecting microcystin-LR in the environment. The developed sensor is high in detection sensitivity, the linear range is 0.1 fg mL <-1 > to 10 pg mL <-1 >, and the detection limit is as low as 0.079 fg mL <-1 >. Development of a biological sensitive OPECT platform brings hope for MC-LR detection, and meanwhile the biological sensitive OPECT platform can be used for detecting other marine pollutants.

The present invention provides an enterotoxin C Raman sensing analysis method based on Au-Ag Janus@Au NPs, belonging to the technical field of Raman spectroscopy analysis. The present invention first synthesizes Au-Ag Janus NPs by guiding the directional growth of Ag islands on Au cores through 2-mercaptobenzimidazole-5-carboxylic acid (MBIA), and the ligand MBIA also acts as a Raman beacon molecule. By regulating the pH, an Au layer is grown on the surface of Au-Ag Janus NPs to obtain Au-Ag Janus@Au NPs, which have a stronger and more stable SERS signal. With MBIA 1278cm ‑1 Using the SERS signal at the site as the detection signal, a Raman sensor for detecting enterotoxin C was designed. The Raman sensor has the advantages of good sensitivity, high accuracy, strong stability and strong anti-interference ability.

The invention discloses a cyclodextrin bionic organic iridium magnetic catalyst and its preparation method and application, belonging to the field of catalysts and applications. The catalyst comprises three parts: magnetic nanoparticles, β-cyclodextrin and adamantane-functionalized 2-pyridine benzamide-type organic iridium catalyst; the magnetic nanoparticles are surface carboxylated magnetic nanoparticles, which are connected to the amino β-cyclodextrin through an amide bond after being activated by N-hydroxysuccinimide; the β-cyclodextrin is connected to the adamantane in the structure of the adamantane-functionalized 2-pyridine benzamide-type organic iridium catalyst through a host-guest coupling effect. It also includes the application of the catalyst combined with a rhodamine 110-type quinone reduction-regulated fluorescent probe in the detection of coenzyme NADH. The cyclodextrin bionic organic iridium magnetic catalyst MBs@CD@Ir prepared by the present invention has excellent biocompatibility, and the constructed NADH fluorescent probe has the advantages of high sensitivity, good selectivity and easy recovery of the catalyst.

The utility model relates to the technical field of lifts, in particular to a bidirectional mechanical trigger type lift car channel safety detection construction lift which comprises a lift car and a guide rail frame and further comprises an ascending channel detection assembly arranged at the top of the lift car and a descending channel detection assembly arranged at the bottom of the lift car. In the ascending process of the lift car, the ascending channel detection assembly touches a foreign matter, the upper portion is pressed, the first limiting switch is triggered, the guide rail frame stops driving the lift car to ascend, and the lift car returns to the initial position after pressure is removed; in the descending process of the lift car, the descending channel detection assembly touches a foreign matter, the lower portion is pressed, the second limiting switch is triggered, the guide rail frame stops driving the lift car to descend, and the lift car returns to the initial position after pressure is removed. Through a bidirectional mechanical trigger structure and an elastic reset design, the device detects foreign matters in an upper channel and a lower channel of the lift car in real time, timely responds and automatically resets, and ensures safe and reliable operation of the construction lift.

The invention belongs to the field of rapid detection of the content of polar components of edible oil, and discloses application of 1, 3-bis (dicyanomethylene) indan in detection of polar components of edible oil. According to the invention, 1, 3-bis (dicyanomethylene) indan is taken as a probe, and the probe has ultra-sensitive polarity response performance. Under the condition of natural light, obvious color change is displayed in edible oil with different polar components, and rapid colorimetric detection of the polar components of the edible oil is realized by naked eyes. The emission peak of the probe is 646 nm, so that the interference of blue-green fluorescence of edible oil is effectively avoided; high correlation exists between the fluorescence intensity at the long wave and the polar component content of the edible oil, so that the accurate determination of the polar component content of the edible oil is realized. The probe realizes colorimetric and fluorescent dual-channel detection, so that the aim of quickly, sensitively and accurately detecting polar components of edible oil is fulfilled. According to the colorimetric method, the polar components of the edible oil can be rapidly detected without any instrument, the cost is low, the operation is simple, and professional skills are not needed, so that the colorimetric method is very suitable for large-scale and on-site accurate determination of the whole chain of food production, storage, transportation, sales and consumption in food factories, market supervision and the like.

This application discloses a road inspection equipment based on elastic waves, relating to the field of road inspection. It includes: a data control system for controlling the excitation of a seismic source system, acquiring and recording simulated seismic signals received by a geophone system and the spatial coordinate data of the geophone system; a geophone system comprising multiple gravity-coupled geophones spaced at intervals along the circumference of a wheeled frame, for receiving simulated seismic signals generated by the seismic source system and coupled to the ground, converting the simulated seismic signals into electrical signals and transmitting them to the data control system; a seismic source system, under the control of the data control system, generating simulated seismic signals and coupling them to the ground; and a wheeled frame for mounting the geophone system and the seismic source system, driving the seismic source system and the geophone system to acquire data on the structure beneath the road during movement. Addressing the low efficiency of deep road defect detection in existing technologies, this application improves the efficiency of deep road inspection.

The invention belongs to the field of life science, and particularly relates to a yersinia quorum sensing system research method, whole-cell biosensor construction based on the method and application of the whole-cell biosensor. The method comprises the following steps: (1) constructing knockout bacteria and YpsR back-supplementing bacteria of a yersinia pseudotuberculosis quorum sensing system YpsR / YpsI; (2) analysis and screening of the affinity of an N-acyl homoserine lactone (AHL) signal small molecule and a YpsR (Yttrium limacinum) regulatory protein, and (3) analysis and screening of the affinity of the N-acyl homoserine lactone (AHL) signal small molecule and the YpsR regulatory protein; (3) construction of a gene line based on AHL and YpsR regulatory protein; and (4) functional verification of the Y.pstb whole-cell sensor based on AHL detection. The biosensor capable of detecting the AHL generated by the yersinia pseudotuberculosis is constructed by researching a YpsR / YpsI quorum sensing system and a molecular signal recognition and combination mechanism, and more theoretical basis and technical support are provided for prevention, control and treatment of the yersinia pseudotuberculosis.

The invention discloses a preparation method and application of an MXene-Au nano composite material multifunctional antibacterial platform. According to the technical scheme, the preparation method is characterized in that a Ti3C2-Au nano composite material is constructed by adopting an electrostatic assembly method in combination with a seed growth method. An ultrathin Ti3C2 nanosheet is stripped from a Ti3AlC2MAX block, gold seeds are loaded by adopting an electrostatic self-assembly method after amination, and composite materials with different gold nanostructures are formed on the Ti3C2 nanosheet by adopting a seed growth method or an electrostatic adsorption effect. The method is simple in process and safe to operate, the synthesized composite material (Ti3C2-Au NWs) of gold nanowire arrays on the two faces of the Ti3C2 nanosheet can achieve sensitive SERS bacterial detection and bacterial adhesion, multiple functions of bacterial capture, SERS detection, photo-thermal sterilization and the like are achieved, and a direction is provided for clinically achieving rapid, sensitive and accurate detection and killing of pathogenic bacteria.

The present invention discloses a method for preparing an organic electrochemical transistor based on laser-induced graphene, and designs and manufactures an all-carbon flexible high-performance OECT based on LIG electrode. Due to the porous structure of LIG, the normalized transconductance of the obtained OECT is as high as 31S cm ‑1 . The high performance achieved is superior to that obtained in Au-based OECTs. The high performance can be maintained after the device shape is deformed. In addition, LIG-based OECTs have much higher cycling stability than Au-based OECTs. In addition, sensitive detection of H2O2 and glucose is demonstrated. By integrating LIG-based OECTs in microfluidic chips, wearable sweat sensors can also be obtained, which are expected to monitor sweat glucose in real time and noninvasively.

The invention belongs to the technical field of biological analysis, and particularly relates to a one-pot method, Cas12a cis-cleavage, in-vitro transcription, a fluorescent RNA aptamer and application of the fluorescent RNA aptamer in microRNA detection in a material sample. The invention provides a method for sensitively and specifically detecting microRNA by connecting, identifying and triggering RPA (recombinase polymerase amplification) amplification and Cas12a cis-cleavage to realize large-scale amplification of a double-stranded DNA (deoxyribonucleic acid) amplicon and transcription of a functional fluorescent RNA aptamer, and combining a mechanism strategy of T7 transcription signal amplification and fluorescent aptamer signal amplification. The invention also provides a method for realizing rapid detection of miRNA-21 by optimizing the reaction process through a one-pot method. By utilizing the high sensitivity, high specificity and label-free characteristics of a detection platform for connecting and recognizing RPA amplification and Cas12a cis-cleavage biosensor through the one-pot method, the miRNA-21 concentration in cancer cells and patient serum samples can be quickly and effectively detected.

The application provides an AI-based charging process safety monitoring method and system, relating to the technical field of charging safety monitoring, comprising obtaining charging state data by collecting current, voltage and temperature data of a charging device, extracting an implicit feature vector using a neural network encoder and projecting it to a feature space to obtain a state representation point, forming a state evolution trajectory from the representation points at consecutive time points and performing shape matching with a reference trajectory to identify an abnormal deviation section, extracting current and temperature data sequences from the charging state data according to the abnormal deviation identifier, performing sliding window sampling to calculate a correlation peak value to determine a conduction time delay amount and a conduction coefficient, and then determining a prediction time window according to the conduction time delay amount and performing phase space reconstruction to calculate a compensation direction vector and a current compensation amount, and generating a control instruction and sending it to the charging device. The application realizes high-precision identification and active compensation control of charging process abnormalities, significantly improving charging safety and equipment operation stability.

The invention discloses a refrigerant leakage detection method of a refrigerant circulation system, a controller and a system thereof.The refrigerant circulation system comprises a first detection module and a second detection module which are adjacently arranged, and the method comprises the steps that in response to starting of the refrigerant circulation system, the first detection module is started, and a first refrigerant concentration value is obtained through the first detection module; and when the first refrigerant concentration value is larger than a first preset threshold value, a second detection module is started, and the measuring range upper limit of the concentration measurement interval of the first detection module is smaller than the measuring range upper limit of the concentration measurement interval of the second detection module. According to the embodiment of the invention, the sensitivity of gas concentration detection can be improved, and the accuracy of gas concentration detection can be improved.

The present invention belongs to the technical field of electrochemiluminescent biosensing, and discloses a method for constructing a reusable nanopore-gated electrochemiluminescent sensor and its application in detecting T-2 toxin. The steps are: 1. Prepare a luminol / Au NPs composite material; 2. Prepare a luminol / Au NPs / CHI / GCE modified electrode; 3. Treat an AAO membrane; 4. Modify the detection area apt / Au-AAO; 5. Construct a nanopore-gated electrochemiluminescent aptamer sensor device for detecting T-2 toxin. 6. Reuse the device. The present invention utilizes an AAO membrane modified with Au NPs to regulate the diffusion of AA, separate the signal output area and the detection area, and test the electrochemiluminescence detection of the change in the luminescence intensity of the luminol / Au NPs luminophore. The present invention provides a detection mode that integrates the advantages of accuracy, rapidity, sensitivity, and reusability.

The invention discloses a preparation method and application of a gold and silver nanocluster coupled DNA fluorescent nanoprobe. Firstly, bovine serum albumin (BSA) is used as a template for reducing Au < 3 + > and Ag < + > to form a metal core NC of the nanocluster, histidine (His) and arginine (Arg) are sequentially added to be physically adsorbed to the surface of the NC, and the nanocluster NCHR with the particle size being about 10 nm is obtained. His molecules can stabilize the NC structure and effectively enhance the fluorescence intensity through hydrogen bonds formed between the His molecules and BSA, electrostatic interaction and the like. Guanidyl in the Arg molecule has a cell membrane penetrating function and can promote rapid uptake of NCHR by cells. The NCHR coupled DNA hairpin h with the optimal fluorescence intensity and cellular uptake effect is selected, and the fluorescence signal of the virus gene can be detected by co-incubating the NCHR-h and the cells for 1 hour, so that the detection time is shortened, and sensitive and rapid virus detection is realized. The extracellular detection range is 10 to 400 nM, and the lowest detection limit is 5.3 nM.

The application provides a coumarin lysosome-targeting probe for hypochlorous acid detection, a preparation method and application thereof. The probe is Cou-CN-Mor, the probe is prepared from 7-diethylamino-4-methylcoumarin and N-bromosuccinimide reaction to obtain compound 2, then reacts with cuprous cyanide to obtain compound 3, then reacts with 4-(4-morpholinyl)benzaldehyde to obtain compound 5, and then reacts with Lawesson's reagent to obtain the probe; the probe is a coumarin fluorescent probe with a large Stokes shift, can realize sensitive detection of HClO, has specific targeting of lysosomes, has low cytotoxicity, and through fluorescence imaging research on biological samples, the probe is successfully applied to detection of HClO in living cells, zebra fish and plant rhizome tissues, and exhibits good bioimaging performance.

The present invention discloses a probe for detecting myrosinase, a preparation method thereof and an application thereof, wherein the method synthesizes amino-thioglucosinolate precursors with different chain lengths by simulating the structure of plant secondary metabolites thioglucosinolates, and the precursor undergoes a nucleophilic substitution reaction with a halogenated active fluorescent parent and then undergoes deprotection to obtain a target compound. The synthesis method of the present invention has simple steps and mild reaction conditions. The probe for detecting myrosinase prepared by the present invention has good water solubility, high stability, good detection selectivity and high sensitivity; after the probe reacts with myrosinase, the absorption and fluorescence spectra are significantly red-shifted, and by monitoring the absorption and fluorescence changes, the detection of myrosinase can be achieved and the difference in enzyme activity can be reflected. The probe for detecting myrosinase of the present invention can be used not only for in vitro myrosinase detection, but also for detection and fluorescence imaging of endogenous myrosinase in plants or flora, and can also be used for myrosinase-related research such as myrosinase inhibitor screening.

A chelating ligand functionalized carbon dot and a high-yield synthesis method and application thereof. The present invention belongs to the technical field of synthesis and application of functional carbon materials. The purpose of the present invention is to solve the technical problems that the existing carbon dots have poor recognition ability for metal ions and the low synthesis yield of the existing carbon dots. The carbon dots of the present invention are synthesized by simultaneous reaction of free radical polymerization and hydrothermal carbonization under the action of an initiator using a small molecule compound containing an unsaturated carbon-carbon double bond and a metal ion chelating agent as precursors. Method: The small molecule compound containing an unsaturated carbon-carbon double bond, the metal ion chelating agent and the free radical initiator are dissolved in water or an organic solvent respectively, and then the above solutions are mixed and placed in a hydrothermal reactor of polytetrafluoroethylene, heated from room temperature to the hydrothermal carbonization reaction temperature and kept warm to synthesize the chelating ligand functionalized carbon dots. The carbon dots of the present invention are used as fluorescent probes for specific recognition of metal ions. The present invention realizes the functionalization of the chelating ligands and high-yield synthesis of carbon dots.

This invention provides a method for chiral recognition of phenylalanine, comprising the following steps: (1) preparing a ReSe2 film using NH4ReO4, NaCl, and selenium powder; (2) preparing gold nanoparticles using HAuCl4, NaBH4, and CTAB and AA at different concentration ratios, and inducing the formation of gold nanoparticles with different chiralities by adding cysteine ​​solutions; (3) dropping the prepared gold nanoparticle colloids with different chiralities onto the above ReSe2 film, and after complete air drying, dropping in a concentration range of 10 ‑3 -10 ‑7 (4) Using a fully automated laser confocal micro Raman spectrometer, Au-ReSe2 substrate materials loaded with phenylalanine of different chiralities were tested. This invention can achieve sensitive detection at a specific deflection angle, and at the same time, through the interaction between the enantiomer and the specific chiral substance, it exhibits a certain difference, which not only enhances the Raman signal, but also achieves good detection range for low concentrations of Phe (10 mol / L). ‑3 -10 ‑7 It has the advantages of strong molecular signal and wide detection range, with chiral recognition capability of mol / L.

The invention discloses a preparation method of a cadmium sulfide-coated tungsten oxide nano array composite material and a method for applying the cadmium sulfide-coated tungsten oxide nano array composite material to hydrogen, and relates to the preparation method of the cadmium sulfide-coated tungsten oxide nano array composite material and the method thereof. The preparation of the nano array material is realized by utilizing a chemical deposition method, the modification and assembly of the nano material are realized by utilizing electrostatic interaction, and finally, the synthesized nano array material is applied to the preparation of the hydrogen sensor, so that the high-sensitivity detection of hydrogen is realized. The method has the advantages of simplicity in operation, high reaction speed, good effect, high selectivity, low cost and the like. The method can be used for hydrogen energy safety monitoring, hydrogen storage and transportation monitoring, hydrogen medical treatment and safety monitoring in related chemistry and chemical engineering fields, and especially has a very strong application prospect in safety monitoring of an ion battery in a new energy automobile and the like.