14results about How to "Achieving Sensitive Detection" patented technology

The invention discloses a preparation method and application of a sensor array based on luminous carbon dots, and relates to the technical field of sensors, the preparation method comprises the following steps: preparing a carbon source which comprises a precursor containing a targeting functional group or a biomolecule derived carbon source, preparing the luminous carbon dots through a chemical synthesis or physical method, modifying the surfaces of the carbon dots, and preparing the sensor array based on the luminous carbon dots. A core-shell structure is added on the surface of a carbon dot by using an in-situ growth method or a layer-by-layer self-assembly method, the carbon dot with the added core-shell structure is fixed on a substrate of a sensor array, and a performance test is carried out. By synthesizing the carbon dots with a targeted recognition function, the selectivity and the sensitivity of the sensor to a target substance can be remarkably improved, and in the synthesis process, the carbon dots can generate specific interaction with the target substance by adjusting factors such as the structure, the shape, the size and the surface chemical property of the carbon dots; therefore, accurate identification of the target substance is realized, and detection of the marker is realized.

The present invention provides an electrochemiluminescent aptamer sensor for detecting synthetic cathinone MDPV, and a preparation method and application thereof, which belong to the field of electrochemiluminescence detection. The electrochemiluminescent aptamer sensor is formed by loading an aptamer SF on the surface of a glassy carbon electrode modified by a composite material In‑MOF@Sn‑TCPP; the composite material In‑MOF@Sn‑TCPP is In‑MOF coated with Sn‑TCPP (5,10,15,20‑tetrakis(4‑carboxyphenyl)tinporphyrin). The detection method is to use a SF / In‑MOF@Sn‑TCPP / GCE modified electrode as a working electrode, an Ag / AgCl electrode as a reference electrode, and a platinum electrode as an auxiliary electrode to form a traditional three-electrode system. The preparation method of the modified electrode in the present invention is simple, and the sensitivity to MDPV detection is high, the selectivity is good, and the linear range is wide.

The present invention belongs to the field of electrochemiluminescence detection, and relates to an electrochemiluminescence aptamer sensor for specific detection of enrofloxacin, as well as a preparation method and application. The sensor is formed by loading an enrofloxacin aptamer on the surface of a glassy carbon electrode modified with a NaYF4-PEI-PTCA nanocomposite material, wherein the NaYF4-PEI-PTCA nanocomposite material is a product of upconversion nanoparticles and perylene tetracarboxylic acid bound by an amide bond; the nucleotide sequence of the enrofloxacin aptamer is apt:5'-CCC ATC AGG GGG CTA GGC TAA CAC GGT TCG GCT CTC TGA GCC CGG GTT ATT TCA GGG GGA-3'. The sensor of the present invention can realize sensitive detection of enrofloxacin, and the minimum detection limit is 3.0×10 ‑15 mol / L.

The application discloses a kind of SPWM control's wireless charging system metal foreign matter detection device and method, including wireless power transmitting unit, wireless power receiving unit, metal foreign matter detection unit and control unit, the wireless power transmitting unit includes the SPWM modulation circuit, inverter circuit and transmitting coil connected in sequence, the metal foreign matter detection unit includes detection coil group, detection module connected in sequence, the detection coil group is laid on transmitting coil;The detection module includes parallel resonance circuit, high-pass filter circuit and differential amplifier circuit connected in sequence;The detection coil of the application can improve the detection sensitivity of small metal foreign matter.

The invention belongs to the technical field of biology, and particularly relates to a promoter responding to pentanediamine, a pentanediamine biosensor and application of the pentanediamine biosensor. The original PpyrF promoter can respond to the concentration of pentamethylene diamine and regulate and control the expression of report protein GFP, the promoter PpyrF-D40 or PpyrF-F5 is obtained by truncation or mutation optimization of the promoter PpyrF, and the dynamic range and sensitivity of the pentamethylene diamine biosensor are improved. The pentane diamine biosensor further constructed on the basis of the promoter PpyrF-D40 or PpyrF-F5 has no responsiveness to a pentane diamine synthesis precursor lysine, so that the biosensor has good specificity when being applied to screening of pentane diamine production strains. The fluorescence intensity value of the pentane diamine biosensor obtained through screening shows an excellent linear response relationship with the concentration of pentane diamine, and the pentane diamine biosensor can be used for efficiently breeding a pentane diamine high-yield strain by being combined with a high-throughput screening system and the like, and has the advantages of high detection efficiency, high sensitivity and the like.

The invention discloses a cyclodextrin bionic organic iridium magnetic catalyst and its preparation method and application, belonging to the field of catalysts and applications. The catalyst comprises three parts: magnetic nanoparticles, β-cyclodextrin and adamantane-functionalized 2-pyridine benzamide-type organic iridium catalyst; the magnetic nanoparticles are surface carboxylated magnetic nanoparticles, which are connected to the amino β-cyclodextrin through an amide bond after being activated by N-hydroxysuccinimide; the β-cyclodextrin is connected to the adamantane in the structure of the adamantane-functionalized 2-pyridine benzamide-type organic iridium catalyst through a host-guest coupling effect. It also includes the application of the catalyst combined with a rhodamine 110-type quinone reduction-regulated fluorescent probe in the detection of coenzyme NADH. The cyclodextrin bionic organic iridium magnetic catalyst MBs@CD@Ir prepared by the present invention has excellent biocompatibility, and the constructed NADH fluorescent probe has the advantages of high sensitivity, good selectivity and easy recovery of the catalyst.

The utility model relates to the technical field of lifts, in particular to a bidirectional mechanical trigger type lift car channel safety detection construction lift which comprises a lift car and a guide rail frame and further comprises an ascending channel detection assembly arranged at the top of the lift car and a descending channel detection assembly arranged at the bottom of the lift car. In the ascending process of the lift car, the ascending channel detection assembly touches a foreign matter, the upper portion is pressed, the first limiting switch is triggered, the guide rail frame stops driving the lift car to ascend, and the lift car returns to the initial position after pressure is removed; in the descending process of the lift car, the descending channel detection assembly touches a foreign matter, the lower portion is pressed, the second limiting switch is triggered, the guide rail frame stops driving the lift car to descend, and the lift car returns to the initial position after pressure is removed. Through a bidirectional mechanical trigger structure and an elastic reset design, the device detects foreign matters in an upper channel and a lower channel of the lift car in real time, timely responds and automatically resets, and ensures safe and reliable operation of the construction lift.

This application discloses a road inspection equipment based on elastic waves, relating to the field of road inspection. It includes: a data control system for controlling the excitation of a seismic source system, acquiring and recording simulated seismic signals received by a geophone system and the spatial coordinate data of the geophone system; a geophone system comprising multiple gravity-coupled geophones spaced at intervals along the circumference of a wheeled frame, for receiving simulated seismic signals generated by the seismic source system and coupled to the ground, converting the simulated seismic signals into electrical signals and transmitting them to the data control system; a seismic source system, under the control of the data control system, generating simulated seismic signals and coupling them to the ground; and a wheeled frame for mounting the geophone system and the seismic source system, driving the seismic source system and the geophone system to acquire data on the structure beneath the road during movement. Addressing the low efficiency of deep road defect detection in existing technologies, this application improves the efficiency of deep road inspection.

The invention belongs to the field of life science, and particularly relates to a yersinia quorum sensing system research method, whole-cell biosensor construction based on the method and application of the whole-cell biosensor. The method comprises the following steps: (1) constructing knockout bacteria and YpsR back-supplementing bacteria of a yersinia pseudotuberculosis quorum sensing system YpsR / YpsI; (2) analysis and screening of the affinity of an N-acyl homoserine lactone (AHL) signal small molecule and a YpsR (Yttrium limacinum) regulatory protein, and (3) analysis and screening of the affinity of the N-acyl homoserine lactone (AHL) signal small molecule and the YpsR regulatory protein; (3) construction of a gene line based on AHL and YpsR regulatory protein; and (4) functional verification of the Y.pstb whole-cell sensor based on AHL detection. The biosensor capable of detecting the AHL generated by the yersinia pseudotuberculosis is constructed by researching a YpsR / YpsI quorum sensing system and a molecular signal recognition and combination mechanism, and more theoretical basis and technical support are provided for prevention, control and treatment of the yersinia pseudotuberculosis.

The application provides an AI-based charging process safety monitoring method and system, relating to the technical field of charging safety monitoring, comprising obtaining charging state data by collecting current, voltage and temperature data of a charging device, extracting an implicit feature vector using a neural network encoder and projecting it to a feature space to obtain a state representation point, forming a state evolution trajectory from the representation points at consecutive time points and performing shape matching with a reference trajectory to identify an abnormal deviation section, extracting current and temperature data sequences from the charging state data according to the abnormal deviation identifier, performing sliding window sampling to calculate a correlation peak value to determine a conduction time delay amount and a conduction coefficient, and then determining a prediction time window according to the conduction time delay amount and performing phase space reconstruction to calculate a compensation direction vector and a current compensation amount, and generating a control instruction and sending it to the charging device. The application realizes high-precision identification and active compensation control of charging process abnormalities, significantly improving charging safety and equipment operation stability.

The present invention belongs to the technical field of electrochemiluminescent biosensing, and discloses a method for constructing a reusable nanopore-gated electrochemiluminescent sensor and its application in detecting T-2 toxin. The steps are: 1. Prepare a luminol / Au NPs composite material; 2. Prepare a luminol / Au NPs / CHI / GCE modified electrode; 3. Treat an AAO membrane; 4. Modify the detection area apt / Au-AAO; 5. Construct a nanopore-gated electrochemiluminescent aptamer sensor device for detecting T-2 toxin. 6. Reuse the device. The present invention utilizes an AAO membrane modified with Au NPs to regulate the diffusion of AA, separate the signal output area and the detection area, and test the electrochemiluminescence detection of the change in the luminescence intensity of the luminol / Au NPs luminophore. The present invention provides a detection mode that integrates the advantages of accuracy, rapidity, sensitivity, and reusability.

The application provides a coumarin lysosome-targeting probe for hypochlorous acid detection, a preparation method and application thereof. The probe is Cou-CN-Mor, the probe is prepared from 7-diethylamino-4-methylcoumarin and N-bromosuccinimide reaction to obtain compound 2, then reacts with cuprous cyanide to obtain compound 3, then reacts with 4-(4-morpholinyl)benzaldehyde to obtain compound 5, and then reacts with Lawesson's reagent to obtain the probe; the probe is a coumarin fluorescent probe with a large Stokes shift, can realize sensitive detection of HClO, has specific targeting of lysosomes, has low cytotoxicity, and through fluorescence imaging research on biological samples, the probe is successfully applied to detection of HClO in living cells, zebra fish and plant rhizome tissues, and exhibits good bioimaging performance.

The present invention discloses a probe for detecting myrosinase, a preparation method thereof and an application thereof, wherein the method synthesizes amino-thioglucosinolate precursors with different chain lengths by simulating the structure of plant secondary metabolites thioglucosinolates, and the precursor undergoes a nucleophilic substitution reaction with a halogenated active fluorescent parent and then undergoes deprotection to obtain a target compound. The synthesis method of the present invention has simple steps and mild reaction conditions. The probe for detecting myrosinase prepared by the present invention has good water solubility, high stability, good detection selectivity and high sensitivity; after the probe reacts with myrosinase, the absorption and fluorescence spectra are significantly red-shifted, and by monitoring the absorption and fluorescence changes, the detection of myrosinase can be achieved and the difference in enzyme activity can be reflected. The probe for detecting myrosinase of the present invention can be used not only for in vitro myrosinase detection, but also for detection and fluorescence imaging of endogenous myrosinase in plants or flora, and can also be used for myrosinase-related research such as myrosinase inhibitor screening.

This invention provides a method for chiral recognition of phenylalanine, comprising the following steps: (1) preparing a ReSe2 film using NH4ReO4, NaCl, and selenium powder; (2) preparing gold nanoparticles using HAuCl4, NaBH4, and CTAB and AA at different concentration ratios, and inducing the formation of gold nanoparticles with different chiralities by adding cysteine ​​solutions; (3) dropping the prepared gold nanoparticle colloids with different chiralities onto the above ReSe2 film, and after complete air drying, dropping in a concentration range of 10 ‑3 -10 ‑7 (4) Using a fully automated laser confocal micro Raman spectrometer, Au-ReSe2 substrate materials loaded with phenylalanine of different chiralities were tested. This invention can achieve sensitive detection at a specific deflection angle, and at the same time, through the interaction between the enantiomer and the specific chiral substance, it exhibits a certain difference, which not only enhances the Raman signal, but also achieves good detection range for low concentrations of Phe (10 mol / L). ‑3 -10 ‑7 It has the advantages of strong molecular signal and wide detection range, with chiral recognition capability of mol / L.