38 results about "Upconversion nanoparticles" patented technology

A bioprobe based on surface-modified single-phase BaGdF5:Yb / Er upconversion nanoparticles (UCNPs) for multi-modal bioimaging of fluorescent, magnetic resonance imaging (MRI) and computed X-ray tomography (CT) is disclosed herein. The modified UCNPs of the present invention are synthesized by a facile one-pot hydrothermal method with simultaneous surface modification of the nanoparticles. The surface-modified UCNPs of the present invention are useful in a variety of biomedical application fields due to their advantages in in vitro and in vivo multi-modal bioimaging such as small particle size up to 15 nm, substantially free of autofluorescence, low cytotoxicity, capable of being excited at near-infrared (NIR) wavelength, ability to deep cell penetration, long-lasting signal and long circulation time in vivo, different X-ray absorption coefficients at different photon energy levels between Ba and Gd, large magnetic moment, etc.

The invention discloses a method for preparing a nanometer system for multi-model diagnosis and treatment integration. The method comprises the following steps: adopting an in-situ growth nanogold modified rare-earth upconversion nanoparticle method to perform ligand exchange modification on oil-soluble upconversion luminescence nanoparticles of citric acid with a core-shell structure to be water-soluble, and performing citric acid ligand modification on the surfaces of the nanoparticles; uniformly growing nanogold particles on the surface through an in-situ crystal growth method so as to obtain in-situ growth nanogold modified upconversion luminescence nanoparticles; and linking polyethylene glycol-adriamycin hydrochloride containing hydrazone bonds and thiol onto the nanogold surface, thereby obtaining the rare-earth upconversion nanometer system. The invention further provides the nanometer system and application thereof. The nanometer system provided by the invention is uniform in size, high in stability and high in biocompatibility, has the effects of chemotherapy and photo-thermal physiotherapy and can be applied to the fields of upconversion fluorescence imaging, magnetic resonance imaging, anti-cancer drug delivery, photo-thermal physiotherapy of cancers and the like.

Belonging to the biological field of material chemistry, the invention relates to a method for double-signal in-situ detection of intracellular microRNA based on a gold-upconversion nanoparticle tetrahedron. The method includes: design of nucleic acid for a detection probe, preparation of a gold-upconversion nanoparticle tetrahedron detection probe, functionalization of the tetrahedron, and establishment of an intracellular microRNA detection method. The method for double-signal in-situ detection of intracellular microRNA established by the invention is used for real-time detection of intracellular microRNA, and the result shows that the CD signal and target microRNA concentration have a good linear relationship in the 0.073-43.65 fmol / 10 microg RNA range, the detection limit can reach 0.03 fmol / 10microg RNA; the linear range of the upconversion luminous intensity and the target microRNA concentration is 0.16-43.65 fmol / 10microg RNA, and the minimum detection limit is 0.12 fmol / 10microg RNA. Therefore, the method is an ultrasensitive and high specificity intracellular microRNA in-situ detection method.

The invention belongs to the technical field of food safety testing immunoassay and relates to a bisphenol A and 17beta-estradiol detection kit based on an upconversion fluorescence aptamer sensor, anapplication and a detection method. The kit comprises (1) first carboxylated core / shell upconversion nanoparticles NaYF4:Yb,Er@NaYF4:Nd, (2) second carboxylated core / shell upconversion nanoparticlesNaYF4:Yb,Tm@NaYF4:Nd, (3) a target aptamer sequence and a regular tetrahedron structure composition sequence designed according to a complementary strand of the aptamer as well as (4) a black phosphorus nanogold composite dispersion, wherein targets comprise bisphenol A and 17beta-estradiol; the regular tetrahedron structure composition sequence comprises a complementary strand sequence of the aptamer sequence, and the at least part of the complementary strand sequence forms the regular tetrahedron structure sequence. With adoption of the kit or the detection method, bisphenol A and / or 17beta-estradiol can be detected with high sensitivity.

The invention provides an amphiphilic polymer with a structure shown in a formula I. The amphiphilic polymer is used for preparing a composite nano drug carrier; the composite nano drug carrier comprises chemically modified Mn<2+> doped rare earth upconversion nanoparticles and the amphiphilic polymer, wherein chemical modification is alkyl chain, silicon dioxide and photosensitizer modification; the chemically modified Mn<2+> doped rare earth upconversion nanoparticles are hydrophobic materials; a near-infrared light is converted into an ultraviolet light by the Mn<2+> doped rare earth upconversion nanoparticles when the composite nano drug carrier is irradiated by using the near-infrared light; the ultraviolet light prompts the photosensitizer to release singlet oxygen; the amphiphilic polymer is oxidized by the singlet oxygen; the ultraviolet light converts the amphiphilic polymer into the hydrophilic polymer, so that the amphiphilic polymer falls off, and the drug can be released. The drug has a good inhibition effect on tumor cells, and the released excess singlet oxygen also has a lethal effect on the tumor cells, so as to achieve the dual treatment effect.

The invention belongs to the field of nanometer material preparation technologies and nanobiotechnology applications and relates to a preparation method of a magnetic upconversion nanocomposite material. According to the preparation method, a DNA molecule is used as a bridge, magnetic nanoparticles are connected with rare earth doped upconversion nanoparticles by utilizing base complementary pairing action to establish a magnetic upconversion nano-composite structure, and the structure both has superparamagnetism and upconversion luminescence characteristic. By adjusting pH value of a system, raising the temperature or adding competitive DNA to remove the magnetic nanoparticles, cancellation to upconversion luminescence by the magnetic components can be eliminated and the detection sensitivity of the upconversion luminescence characteristic can be improved. The method is simple, operation is convenient and quick, and the synthesized magnetic upconversion nanocomposite material can be applied to bio-medical fields of bioseparation, bioinstrumentation, upconversion luminescence imaging, and the like.

The invention belongs to the technical field of luminous materials and relates to a nanogold shell coated upconversion nanocrystal composite structure material and a preparation method of the material. The material solves the technical problems of large nano particle size, low fluorescence intensity, incomplete core-shell structure and poor biological tissue penetration capacity of the existing rare-earth upconversion luminescent material. The invention provides the nanogold shell coated upconversion nanocrystal composite structure material in a fluoride and oxide upconversion nano core-shellstructure. The invention further provides the preparation method of the composite structure material. Compared with the upconversion luminescent material prepared by the traditional method, the nanogold shell coated upconversion nanocrystal composite structure material is small in particle size, regular in pattern, good in dispersity, good in biocompatibility, and easy in surface biological functionalization; a gold shell is ultrathin, complete and consecutive; and selectively reinforced fluorescence emission is obtained by adjusting a position of an SPR (surface plasmon resonance) absorptionpeak. The preparation method of the material has the advantages of good controllability, low reaction temperature, low equipment requirements, simple in-situ synthesis operation technology and the like.

The invention relates to a preparation method and application of a gold-upconversion nanoparticle tripolymer, belonging to the technical field of analytical chemistry. The preparation method mainly comprises the following steps: synthesis of gold nanoparticles with the particle size of 20nm, modification of Mucin-1 aptamer on gold nanoparticles, modification of AFP aptamer on upconversion nanoparticles, modification of AFP and Mucin-1 aptamer partial complementary sequences on gold nanoparticles, assembly of gold-upconversion nanoparticle tripolymer, and fluorescent and Raman detection. After the AFP is applied, the fluorescence can be enhanced; and after the Mucin-1 is applied, the Raman signal becomes weak, thereby establishing the standard curve between fluorescent signals and AFP concentration and the standard curve between Raman signals and Mucin-1 concentration. The invention provides a multiplex supersensitive detection method for cancer biomarkers by using the gold-upconversion nanoparticle tripolymer. Compared with the traditional detection methods, the multiplex supersensitive detection method has the advantages of low cost and high sensitivity, is convenient and quick, and has favorable practical application prospects.

The invention provides a nano carrier for near-infrared light triggered release of a chemotherapy drug and a preparation method thereof. The preparation method comprises the steps: polymerizing a photosensitive monomer, a hydrophilic monomer and a hydrophobic monomer, to prepare a photosensitive polymer; providing oleic acid modified upconversion nanoparticles, mixing the photosensitive polymer with the upconversion nanoparticles, and allowing the photosensitive polymer to act on the upconversion nanoparticles, to prepare the nano carrier. The nano carrier for near-infrared light triggered release of the chemotherapy drug is composite upconversion nanoparticles having good hydrophilia, and has a structure with a single-layer shell wrapping multiple cores, so that the nanoparticles can quickly response to near-infrared light and accurately release the drug in vivo, and the problem of low release efficiency of general drug loaded nanoparticles is overcome.

The invention discloses a nano-prodrug of light-induced self-produced oxygen, singlet oxygen and active divalent platinum for the treatment of hypoxic tumors. The nano-prodrug is a hydrophobic photosensitizer via a hydrophilic linker and Azido-containing tetravalent platinum [Pt(N 3 ) 2 (OH)(O 2 C(CH 2 ) n COOH)(Am1) 2 ] Linked amphiphilic photodynamic-chemotherapy combined anticancer prodrugs, self-assembled into nanoparticles in water, and loaded with up-conversion nanoparticles. The invention decomposes under the light of 980 nanometers to produce oxygen and divalent platinum with anticancer activity, and the oxygen produced by itself can be utilized by the photosensitizer in the prodrug to generate singlet oxygen, and finally singlet oxygen and anticancer activity The combination of active divalent platinum achieves the effect of photodynamic-chemotherapy combined therapy, thereby reversing the resistance of tumor hypoxic areas to photodynamic therapy and improving the therapeutic effect of tumors; at the same time, it is particularly stable in the absence of light and can reduce Toxic side effects on normal cells.

The invention discloses a fluorescence detection test strip for simultaneously detecting four types of pathogenic bacteria, and a preparation method and application thereof. The bottom layer of the test strip is a supporting plate; the supporting plate is pasted with a sample cushion, a combination cushion, a nitrocellulose membrane and an adsorption cushion in sequence, wherein the sample cushion, the combination cushion, the nitrocellulose membrane and the adsorption cushion are tightly connected; the nitrocellulose membrane is provided with four detection lines and one quality control linein sequence; the combination cushion is coated with an upconversion nanoparticle-pathogenic bacteria first aptamer compound; the detection line is independently coated with a pathogenic bacteria second aptamer-streptavidin compound, i.e., a capture probe; and the quality control line is coated with a polyT-sequence-streptavidin compound, i.e., a quality control probe. The test strip has the advantages of high sensitivity, good specificity and low cost, can realize the simultaneous detection of four types of pathogenic bacteria in a complex sample, and has a good application prospect.

The invention belongs to the technical field of food safety detection immunoassay and relates to an atrazine detection kit based on an upconversion fluorescence immunoassay sensor, application and anatrazine detection method. The kit comprises carboxylation core / shell type upconversion nanoparticles NaYF4:Yb,Er@NaYF4:Nd, anti-atrazine monoclonal antibody and black phosphorus nanogold composite material dispersion liquid. By utilizing the kit or the detection method, high-sensitivity detection of atrazine can be realized.

The invention relates to a fibrotic paper sensor based on methylene blue and up-conversion nanoparticle modification and a preparation method thereof, which is used for detecting the activity of telomerase, a tumor marker. When the cell lysate to be tested is dropped on the modified fiber paper, the telomerase substrate can undergo different degrees of elongation reaction, and then use oligonucleotide-modified methylene blue and up-converting nanoparticles to telomere The enzyme extension product is reacted, and the detection result can be obtained by the color depth of methylene blue and the fluorescence intensity of the up-conversion nanoparticles under near-infrared irradiation. The fixed fibrous paper can better carry the detection liquid through capillary action, which is beneficial to the occurrence and progress of biological reactions. The present invention has the advantages of simple operation, low preparation cost, fast and accurate detection, strong specificity, and high sensitivity. Good application prospects.

The invention discloses a luminous color variable upconversion nanometer luminescent material, which has a chemical expression of NaR0.8-x-yF4: Yb0.2, Hox, Tmy; in the chemical expression, R is at least one of Y, Gd and Ce, x is more than or equal to 0.0001 and less than or equal to 0.1, and y is more than or equal to 0.0005 and less than or equal to 0.1; the diameters of upconversion nanoparticles are 5-25nm. The material can be divided into two types according to the distribution mode of an activating agent: a single-core structure and a core-shell structure, wherein in the single-core structure, Tm and Ho are evenly distributed in nanoparticles, and in the core-shell structure, Tm and Ho are respectively distributed in a core and a shell. The invention also discloses a preparation method of the upconversion nanometer luminescent material. By using the surface effect of the upconversion nanometer luminescent material, the upconversion luminescence color of the material can change obviously along with the change of laser power density, temperature and radiation time under the condition of 980nm near infrared excitation, so that the material can be applied to the anti-counterfeit field.

The invention discloses a lattice defect-control orthogonal-emission rare earth upconversion nanoparticle and a preparation method. Through co-doping of metal ions such as Mn<2+> and Tm<3+> with Er<3+> ion as a luminescent center, defects are introduced into lattices of an upconversion nanomaterial, and the pathways of energy transmission under 980nm excitation and 808nm excitation are controlled,wherein under 980nm laser excitation, the pathway of the energy transmission is Yb<3+> to Er<3+> to Mn<2+>(Tm<3+>) and red fluorescence is emitted, and under 808nm laser excitation, the pathway of the energy transmission is Nd<3+> to Yb<3+> to Er<3+> and green fluorescence is emitted. Through regulation of the doping amount of Mn<2+>, Tm<3+> or other metal ions, emission of the fluorescence withdifferent colors by the same luminescent center Er<3+> ion under different excitation wavelengths can be effectively controlled. The property of orthogonal emission of the same nanoparticle has a wideapplication prospect in the field of biomedicine.

The invention provides an ochratoxin upconversion fluorescent sensing material. The invention also discloses a preparation method of the sensing material. The method is as below: (1) adding ochratoxin, functional monomer 3-aminopropyltriethoxysilane into a methanol solution, stirring to fully dissolve and react, then adding the upconversion nanoparticles (UCNPs), stirring for 50 min, then adding tetraethoxysilane and a catalyst acetic acid, and incubating in a water bath at 30 DEG C for 12 h; (2) conducting pumping filtration, washing away unreacted materials by a PBST solution, conducting vacuum drying and aging at 50 DEG C for 10 h; placing the polymer in a Soxhlet extractor, washing away the ochratoxin by using 2% trichloroacetic acid PBST mixed solution as a solvent, and conducting vacuum drying a 50 DEG C to obtain a fluorescent molecular imprinting material with high selectivity for ochratoxins. The invention has the advantages that the molecular imprinting sensing material synthesized by the sol-gel method uniform grain size, high selectivity on ochratoxin, and broad application prospects.

The invention discloses a near-infrared light-controlled medicine carrier, namely a carrier with medicine carrying ability which is formed by o-nitrobenzyl ester amphiphilic molecule vesicles encapsulating water-soluble upconversion nanoparticles (Ligand-UCNPs), and belongs to the field of surfactants. The total number of alkyl carbon atoms of a fatty chain is 6 to 36. A preparation method of thecarrier comprises the following three steps of synthesizing oil-soluble upconversion nanoparticles (OA-UCNPs); synthesizing the water-soluble upconversion nanoparticles (Ligand-UCNPs); encapsulatingthe upconversion nanoparticles by the o-nitrobenzyl ester amphiphilic molecules. The near-infrared light-controlled medicine carrier has the advantages that the o-nitrobenzyl ester amphiphilic molecules have ultraviolet light crackability; by introducing the upconversion nanoparticles (UCNPs) which have large tissue penetration depth and can convert the near-infrared light with little injury to ahuman body into the ultraviolet light, the defects of small penetration depth and large injury to the human body of the ultraviolet light are overcome, and the purpose of medicine release by photolysis is realized; the near-infrared light-controlled medicine carrier is a potential near-infrared light-controlled medicine release carrier.

The invention discloses a method for controllably preparing a strong-luminescence rare earth up-conversion material, which comprises the following steps: blending and heating two rare earth up-conversion luminescent materials with different sizes at high temperature to prepare nanoparticles with uniform and stable sizes. Compared with a rare earth up-conversion luminescent material with the same size prepared by the traditional solvothermal method, the advantage is that the fluorescence intensity is increased. According to the invention, the common problem of low luminous efficiency of up-conversion nano materials is solved; the preparation method can effectively enhance the luminous efficiency of the up-conversion material, provide a simple, feasible and controllable new method for preparing the high-efficiency luminous up-conversion material, simplify the operation steps of the traditional method for improving the luminous efficiency of the up-conversion material, and is convenient for batch production of the up-conversion nano material with high-efficiency luminescence and uniform particle size.

The invention relates to a method for preparing a nanoparticle dual-mode fluorescent probe by virtue of a co-assembly technology and belongs to the technical field of nano fluorescent probe preparation. The method comprises the following steps: firstly removing residual ligands from upconversion nanoparticles of an oil phase and supplementing a sulfydryl cadmium carboxylate complex to downconversion nanoparticles of the oil phase; mixing the upconversion nanoparticles of the oil phase and the downconversion nanoparticles of the oil phase and co-assembling two nanoparticles by absorbing upconversion nanoparticles of the oil phase by using the downconversion nanoparticles of the oil phase through continuous lucifugal stirring treatment. Co-assembly brings two results: one is that the upconversion nanoparticles are transferred from the oil phase to the water phase; the other one is that the dual-mode fluorescent probe is formed. The method provided by the invention is simple in integral operating process, less in dangerousness and high in yield of dual-mode fluorescent probe and has good experimental repeatability.

The invention provides a nano carrier with a tumor targeted wrinkled core-shell structure. The nano carrier is prepared by sequentially wrapping outer surfaces of rare-earth upconversion nanoparticles with an upper silicon dioxide layer and a 3-amino isopropyl trimethoxy silane thin layer APTMS from inside to outside, and performing etching treatment on the 3-amino isopropyl trimethoxy silane thin layer. The nano carrier with the tumor targeted wrinkled core-shell structure comprises hyaluronic acid for targeting, the thin layer SiO2 / APTMS as a wrinkle layer, and the upconversion nanoparticles as a core. A winkled layer has the advantages of being high in flexibility, large in surface area, short in distance with the upconversion nanoparticles on the core, and the like, light-sensitive molecules can be effectively carried for light power treatment, or fluorescent molecules can be carried for imaging.

The invention discloses a preparation method of mulberry silk capable of fluorescing under irradiation of near-infrared light, including the steps of (1), preparing upconversion nanoparticles, performing surface modification with concanavalin to obtain modified upconversion nanoparticles; (2), uniformly dispersing the upconversion nanoparticles in water to prepare aqueous solution of upconversionnanoparticles; (3), picking mature mulberry leaves, soaking the mulberry leaves in the aqueous solution of upconversion nanoparticles, draining water and then drying in air naturally; (4), feeding silkworms the processed mulberry leaves until the silkworms spin and make cocoons when the silkworms grow to the designed stage; (5), collecting silk so as to obtain the mulberry silk capable of fluorescing under irradiation of near-infrared light. According to the preparation method, the upconversion nanoparticles applied to preparation is capable of fluorescing under irradiation of near-infrared light having higher penetrating power, and accordingly has better application in imaging of deep tissue. Besides, the upconversion nanoparticles have much stable property, higher bio-security, higher signal to noise ratio and wider application range.

The invention relates to an antitumor composition based on hydrophobized hyaluronan and inorganic nanoparticles stabilized by oleic acid. The hydrophobized hyaluronan in the form of an acylated hyaluronan serves in the composition as a carrier of inorganic nanoparticles. Out of the group of inorganic nanoparticles, the composition may comprise superparamagnetic nanoparticles, nanoparticles of ZnO and moreover, upconversion nanoparticles. Said composition is selectively cytotoxic with respect to both suspension and adherent tumor cell lines, especially with respect to tumor cell lines of colorectum carcinoma and adenocarcinoma, lung carcinoma, hepatocellular carcinoma and breast adenocarcinoma. The highest cytotoxic effects were observed in case of the composition based on an oleyl derivative of hyaluronan with SPIONs. The composition of acylated hyaluronan with SPIONs may also be advantageously used for an in vivo detection of accumulation of the composition in the body, preferably in a tumor or in liver. Said composition is sterilizable in the final package.

The invention discloses a sound-control nanoparticle-loaded berberine releasing system and a preparation method and application thereof. The sound-control nanoparticle-loaded berberine releasing system comprises upconversion nanoparticles which are amino-modified in surfaces and clad through SiO2, a singlet oxygen sensitive switch, hyaluronic acid and berberine; one end of the singlet oxygen sensitive switch is coupled to the upconversion nanoparticles which are amino-modified in surfaces and clad through SiO2 through amido bonds, the other end of the singlet oxygen sensitive switch is coupled to the hyaluronic acid through ester bonds, the berberine is loaded in the upconversion nanoparticles which are amino-modified in surfaces and clad through SiO2 in a wrapped or covalent linkage mode, and the berberine releases singlet oxygen under the ultrasonic action, so that a carbon-carbon double bond in the singlet oxygen sensitive switch is ruptured, the berberine is released, and aggregation of medicine in non-target tissues can be reduced. Raising of the sound-control nanoparticle-loaded berberine releasing system fills up the blank of sound-sensitive agent carriers in SDT research, and application of a sound-control nanoparticle drug loading system to SDT is achieved.

Provided is an in vivo bioimaging method including irradiating near-infrared (NIR) light onto a living body, converting the NIR light passed through the living body, into visible light using upconversion nanoparticles (UCNPs), and generating a bioimage of the living body by receiving the visible light using a complementary metal-oxide-semiconductor (CMOS) image sensor.

The invention discloses a method for constructing a multifunctional upconversion nano-platform. The method includes loading antibodies or multifunctional human serum albumin on the surfaces of water-soluble upconversion nanoparticles through chemical bonding, and loading one or more fluoresceins on the surfaces of active groups such as the antibodies through hydrophobic interaction between proteins and fluorescein molecules so as to construct the multifunctional upconversion nano-platform. Compared with the prior art, the method has the advantages that a nano-composite prepared by the method is highly targeted, good in biocompatibility, simple in technological process, low in cost and easy to popularize and apply on a large scale, and the loaded fluorescein molecules are high and adjustable in concentration; the method has a promising application prospect in the field of biomedicine, tumor imaging and targeted delivering of anti-cancer drugs in particular.

The invention belongs to the field of luminescent materials and relates to a submicron-scale lumpy calcium titanate based upconversion luminescent material and a preparation method thereof. The submicron-scale lumpy calcium titanate based upconversion luminescent material is used for solving the technical problem in the prior art that prepared calcium titanate based rare-earth upconversion luminescent materials are non-uniform in size, irregular in shape, high in preparation temperature and difficult in doping. According to the preparation method provided by the invention, the raw materials are cheap, the preparation process is simple and convenient, the operation is easy, the calcination temperature is low, and the production facilities are readily available. According to the upconversionluminescent material, the morphology, size and luminescent intensity of prepared rare-earth doped CaTiO3:Yb<3+> / Er<3+> or Ho<3+> can be regulated and controlled through changing a solvothermal environment, and the upconversion luminescent material is high in purity, uniform in size, regular in shape and relatively good in dispersity and has the average side length of 500 nm. Bright visible lightwith the wavelength of 400 nm to 750 nm can be viewed under the excitation of infrared light with the wavelength of 980 nm. Compared with calcium titanate upconversion luminescent materials prepared by the traditional methods, the upconversion luminescent material has the advantages that the light emitting brightness is further improved, and the material is stable in physico-chemical properties; the luminescence efficiency is also higher than that of commonly-used titanate materials.