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Nano carrier with tumor targeted wrinkled core-shell structure

A nano-carrier and nano-particle technology, applied in the direction of medical preparations containing non-active ingredients, medical preparations containing active ingredients, inorganic non-effective ingredients, etc., can solve the problem of increasing the size effect of nano-carriers, not easy to deform, reducing flexibility, etc. question

Active Publication Date: 2017-05-10
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In the current field of preparation of rare earth-targeted upconversion nanocarriers, the core-shell structure is the main nanocarrier construction method, but the problems with this structure mainly include that the shell layer of the nanocarrier is generally in a fixed shape and is not easily deformed, such as silica layer
The fixed shell of nanocarriers will increase the size effect of nanocarriers in vivo, reduce the flexibility of in vivo transportation and tissue absorption, and increase the frictional damage to blood vessels and tissues in vivo

Method used

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  • Nano carrier with tumor targeted wrinkled core-shell structure

Examples

Experimental program
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Effect test

Embodiment 1

[0026] The preparation of the nano-carrier of the wrinkled core-shell structure of embodiment 1 targeting

[0027] 1) Add 0.1ml CO-520 into cyclohexane containing 0.004M upconversion nanoparticles, and stir for 10min. Then add 0.4ml of CO-520 and 0.08ml of 28wt% ammonia water, seal the solution and sonicate it for 20min until the solution becomes clear. Then add 0.04ml of TEOS, and the solution is sealed and rotated for 2 days. After the rotation, add acetone for precipitation, wash twice with ethanol / water (v / v=1:1) mixture and store in 1.5ml of absolute ethanol.

[0028] 2) Dissolve UCNPs@SiO in absolute ethanol 2 , centrifuge at 10000rpm for 10min, discard the supernatant, add 1.5ml of toluene, shake for 5min, centrifuge at 10000rpm for 10min, remove the supernatant. The nanoparticles were aspirated into a vial containing 4ml of toluene and spun at 150rpm for 6h. After that, 90ulAPTMS was added, and the solution was sealed and rotated at room temperature for 24h. Centr...

Embodiment 2

[0032] The preparation of the nano-carrier of the wrinkled core-shell structure of embodiment 2 targeting

[0033] 1) Add 0.1ml CO-520 into cyclohexane containing 0.01M upconversion nanoparticles, and stir for 10min. Then add 0.4ml CO-520 and 0.08ml, 28wt% ammonia water, seal the solution and sonicate for 20min until the solution becomes clear. Then add 0.04ml of TEOS, and the solution is sealed and rotated for 2 days. After the rotation, add acetone for precipitation, wash twice with ethanol / water (v / v=1:1) mixture and store in 1.5ml of absolute ethanol.

[0034] 2) Dissolve UCNPs@SiO in absolute ethanol 2 , centrifuge at 10000rpm for 10min, discard the supernatant, add 1.5ml of toluene, shake for 5min, centrifuge at 10000rpm for 10min, remove the supernatant. The nanoparticles were aspirated into a vial containing 4ml of toluene and spun at 150rpm for 6h. After that, 120ul APTMS was added, and the solution was sealed and rotated at room temperature for 24h. Centrifuge a...

Embodiment 3

[0038] Example 3 Application of 1UCNPs@thin-layer SiO2 / APTMS wrinkled core-shell nanocarriers loaded with photosensitizers for photodynamic therapy

[0039] 1) Weigh 0.5 mg of titanium dichloride phthalocyanine and dissolve it in 3 ml of pyridine solution, seal and sonicate for 40 min. Immerse the wrinkled core-shell nanoparticles obtained in step 3) into the supernatant, seal the mouth of the bottle and sonicate for 40 minutes, then rotate at room temperature at 600 rpm for 24 hours. Nanoparticles were collected by centrifugation and centrifuged at 3000rpm for 10min. Take 2ml of PBS solution (0.1M pH7.5) and pipette the solution until the particles are evenly mixed and the solution becomes a suspension, then centrifuge to collect the nanoparticles, and centrifuge at 3000rpm for 10min. Repeat the above steps and wash the nanoparticles again with 2ml of PBS solution. Finally the particles were deposited in 2ml MES buffer. Obtaining Nanoparticles Loaded with Photosensitizer T...

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Abstract

The invention provides a nano carrier with a tumor targeted wrinkled core-shell structure. The nano carrier is prepared by sequentially wrapping outer surfaces of rare-earth upconversion nanoparticles with an upper silicon dioxide layer and a 3-amino isopropyl trimethoxy silane thin layer APTMS from inside to outside, and performing etching treatment on the 3-amino isopropyl trimethoxy silane thin layer. The nano carrier with the tumor targeted wrinkled core-shell structure comprises hyaluronic acid for targeting, the thin layer SiO2 / APTMS as a wrinkle layer, and the upconversion nanoparticles as a core. A winkled layer has the advantages of being high in flexibility, large in surface area, short in distance with the upconversion nanoparticles on the core, and the like, light-sensitive molecules can be effectively carried for light power treatment, or fluorescent molecules can be carried for imaging.

Description

technical field [0001] The invention belongs to the technical field of preparation of targeted nano-carriers, and in particular relates to a nano-carrier with a non-fixed shell layer. Background technique [0002] In the current field of preparation of rare earth-targeted upconversion nanocarriers, the core-shell structure is the main nanocarrier construction method, but the problems with this structure mainly include that the shell layer of the nanocarrier is generally in a fixed shape and is not easily deformed, such as silica layers etc. The fixed shell of nanocarriers will increase the size effect of nanocarriers in vivo, reduce the flexibility of in vivo transportation and tissue absorption, and increase the frictional damage to blood vessels and tissues in vivo. At the same time, the method of loading molecules on nanocarriers is usually by physical immersion. The shell with a fixed shape has a limited amount of immersion into the loaded molecules, and the loading of ...

Claims

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Application Information

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IPC IPC(8): A61K9/50A61K47/02A61K47/24A61K47/36A61K41/00A61K49/00
CPCA61K41/0057A61K47/36A61K49/0052A61K49/0093A61K9/501A61K9/5015
Inventor 刘学庆燕照霞刘涵云杨丽敏马洪超
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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