10 results about "Ochratoxin" patented technology

This invention relates to a formulation and method of application of a multicomponent mycotoxin detoxifier for poultry feed, particularly female broiler chickens, which aims to provide protection to the Bursa Fabricius due to exposure to mycotoxins in the form of aflatoxin and ochratoxin. The mycotoxin detoxifier formulation according to this invention consists of an inorganic mycotoxin binder in the form of bentonite and / or zeolite, an organic mycotoxin binder in the form of pectin and / or esterified cellulose, and a natural antioxidant in the form of vitamin E and / or antioxidant herbal extracts. The formulation is applied to poultry feed at a dose of 2 to 2.5 g per kg of feed and is able to bind aflatoxin and ochratoxin simultaneously in the digestive tract, thereby reducing the bioavailability of mycotoxins.The application of this formulation has been proven to reduce histopathological damage to the Bursa of Fabricius, including lymphoid follicle depletion, cyst formation, cell necrosis, inflammatory cell infiltration, and tissue fibrosis, while also supporting the immune system function of female broiler chickens. This invention provides an effective, efficient, and easily applicable technical solution in the poultry farming industry to address mixed mycotoxin contamination in feed.

The invention relates to the technical field of silage, and provides a silage additive for degrading mycotoxin and application of the silage additive. The composite microbial inoculant is composed of lactobacillus paracasei and bacillus subtilis; or the microbial agent is a composite microbial agent composed of lactobacillus paracasei, bacillus subtilis and pediococcus pentosaceus. By screening degrading bacteria aiming at ochratoxin, aflatoxin, zearalenone and vomitoxin for compounding and combination, the degrading effects of different lactic acid bacteria on various toxins are respectively exerted, the various mycotoxins are effectively degraded, and particularly, the degrading rate of the ochratoxin can reach 100%. The degradation rate of the mixed bacteria to other mycotoxins can exceed that reported in the prior art, and faster degradation or higher degradation rate can be achieved by adjusting the addition amount.

The invention discloses an ochratoxin compound with aquatic pathogenic bacterium resisting activity and a preparation method and application thereof, the ochratoxin compound is characterized in that the structural formula of the compound is shown as I, and the preparation method comprises the following steps: firstly, obtaining a fermentation product of marine fungi through fermentation culture; soaking the fermented product with ethyl acetate, and extracting to obtain a crude extract; the preparation method comprises the following steps: firstly, preparing a crude extract, then separating and purifying the crude extract by using normal-phase silica gel column chromatography, reverse medium-pressure column chromatography and reversed-phase semi-preparative high performance liquid chromatography to finally obtain the target ochratoxin, and the target ochratoxin has the advantages that the target ochratoxin has a remarkable effect of resisting the activity of aquatic pathogenic bacteria and can be used for research and development of drugs for treating aquatic mycosis.

The invention discloses an amide hydrolase and an application of the amide hydrolase in degradation of ochratoxin. The invention provides a protein or a mutant thereof. The protein or the mutant thereof comprises a protein as shown in an amino acid sequence of SEQ ID NO: 1; or a protein which is obtained by mutating the 76th amino acid residue S on the amino acid sequence as shown in SEQ ID NO: 1 into E and has the same function; or a protein which is obtained by mutating the 334th amino acid residue V on the amino acid sequence as shown in SEQ ID NO: 1 into E and has the same function as the amino acid residue V on the amino acid sequence as shown in SEQ ID NO: 1; or the N end or / and C end of each protein is / are connected with a tag to obtain a protein shown as a sequence. The invention finds that the amide hydrolase ThADH and the mutant can efficiently degrade OTA (Ochratoxin A) and realize OTA detoxification.

The present invention provides a strain of brown metarhizium IFST-OT3 and the detoxification enzyme produced by it and its application. The present invention provides a strain of brown metarhizium IFST-OT3 (deposit number CGMCC No. 40349) with strong degradation ability for ochratoxins A and B, as well as the detoxification enzyme (degradation enzyme) Amh1 produced thereby and the nucleotide sequence encoding the amidase, and also provides the optimal physicochemical properties of the enzyme, including the influence of temperature, pH and metal ions. The amidase treats a substrate concentration of 1 μg / ml OTA toxin in a buffer system at 60°C and pH 6.0, and the degradation efficiency is as high as 100% within 3 minutes. Amh1 has strong adaptability to temperature and acidic pH. The strain IFST-OT3 and the detoxification enzyme produced thereby can be used for the degradation of ochratoxins in agricultural products, and has broad application prospects.

The invention discloses a mycotoxin degrading bacterial agent and application thereof in traditional Chinese medicine storage. According to the microbial agent, bacillus coagulans is used as a core degradation component of ochratoxin, tea residues are used as a carrier, microbial cellulose is generated through kombucha mother fermentation to form a nanometer net-shaped structure, meanwhile, rich pores are formed in the tea residues, an efficient adsorption interface and a diffusion channel are provided for OTA, and an ideal colonization microenvironment is provided for bacillus coagulans. Quercetin can inhibit the synthesis of ochratoxin, and forms a synergistic antibacterial effect with a kombucha fermentation product and bacillus coagulans, so that fungal pollution is doubly controlled from the source and in the process. The microbial agent carrier is stable in structure, can realize sustained release of quercetin and continuous biodegradation of thalli, is natural in raw materials, and is suitable for prevention and control of mycotoxin in a traditional Chinese medicine storage process.

The present invention discloses a method for determining fumonisins and ochratoxins in cereals, comprising: adding cereal powder to a mixed isotope internal standard, then adding an extracting solution and vortexing and stirring for extraction, subjecting the extracting solution to low-temperature centrifugation, and after phase separation, removing a lower layer solution to obtain the extracting solution; the extracting solution is an acetonitrile-water solution containing aqueous ammonia. The method of the present invention adopts a low-temperature centrifugation induced phase separation integrated method to achieve a one-step operation process integrating target extraction, enrichment, purification and centrifugation, and by optimizing the extracting solution, fumonisins and ochratoxins of different polarity properties can be simultaneously transferred to the same small volume collection layer without the need for additional tedious manual operations, greatly improving the extraction, enrichment and purification efficiency, having the advantages of simple operation, short time consumption and low cost, and providing a new determination method for sample preparation and detection of fumonisins and ochratoxins in cereals.

The present invention provides a method for preparing an adsorbent for efficiently enriching multiple mycotoxins in cereals. The method adopts polydopamine and 1-carboxyethyl-3-butylimidazolium bromide dual-modified magnetic nanoparticles as a novel adsorbent to enrich multiple types of mycotoxins in cereals, such as aflatoxins, ochratoxins, fumonisins, zearalenone toxins, penicillin toxins, sterigmatocystin, and Class A and Class B trichothecenes toxins. The method reduces the co-adsorption of matrix components in the cereals, and provides a sample to be tested in which the target is enriched and interference is purified for subsequent analysis and detection.

The application discloses a product for degrading ochratoxin and a special protein. The application provides a protein or a mutant thereof, which is any one of the following: a protein as shown in an amino acid sequence of SEQ ID NO: 1; or a protein in which an amino acid residue S at the 66th position in the amino acid sequence shown in SEQ ID NO: 1 is mutated into E and the protein has the same function; or a protein in which an amino acid residue V at the 324th position in the amino acid sequence shown in SEQ ID NO: 1 is mutated into E and the protein has the same function; or a protein in which a label sequence is connected to the N terminal or the C terminal of each of the above proteins. The application finds that an amide hydrolase VeADH and a mutant thereof can efficiently degrade OTA, and OTA detoxification is realized.

The invention discloses a full-automatic soybean ochratoxin detection device and a use method, the full-automatic soybean ochratoxin detection device comprises three areas on a workbench, namely a sample area, a mixing area and a reaction area; a plurality of test tubes for loading samples to be detected are placed in the sample area; a material mixing mechanism is arranged in the material mixing area; a constant-temperature incubator is arranged in the reaction area; a flexible clamping jaw is integrated at the end part of the mechanical arm on one side of the workbench, and a test tube is grabbed by the flexible clamping jaw to move among the three areas; the sampling needle is used for absorbing the reacted sample for multiple times; the titrating head is used for adding a detection reagent into the test tube loaded with the sample to be detected; and the identification camera is used for identifying the labels of the test tubes through shooting of the identification camera so as to distinguish different to-be-detected samples. By integrating the flexible clamping jaw, the sampling needle, the titrating head and the identification camera, unmanned detection of the whole process of loading, mixing, reacting and sampling of a sample is realized, the labor cost is remarkably reduced, the detection efficiency is improved, and meanwhile, automatic clean detection is really realized.