93 results about "Ochratoxin" patented technology

The invention discloses a method for detecting ochratoxin A by the magnetic separation of an aptamer-functionalized magnetic nano material and the marking of an up-conversion fluorescent nano material, which is used for detecting the ochratoxin A content of wheat, grains, feeds and products thereof, and the like. In the method, the ochratoxin-specific aptamer-functionalized magnetic nano material is utilized to perform magnetic separation and enrichment reaction on a sample to rapidly condense bulk samples, effectively increase the condensation of the samples by thousands of times, greatly shorten a detection period and improve the detection sensitivity; and simultaneously, the novel ochratoxin A analysis method for realizing rapid sample treatment, analysis and detection is constructed by combining the characteristics of high sensitivity of laser induced fluorescence of the up-conversion fluorescent nano material and capability of effectively avoiding the interference of biological background fluorescence of the samples.

A feed nano additive for adsorbing fungal toxin in feed is prepared through proportionally mixing montmorillonite, sodium chloride and water, stirring, water washing, preparing pulp again; adding glucomannan while high-speed stirring, reaction, ageing, water washing, dewatering; drying filter residue and breaking. Its advantages are broad-spectrum adsorption of different toxins, high adsorptive power, no influence of pH value, low residual and low dosage (0.02-0.2%).

The invention provides a preparation method for hydroxylation of 11 alpha of an important intermediate of a steroidal hormone substance, and aims to solve the problems that the conversion rate is low and the environment is polluted when a microorganism is used for converting steroidal C11 alpha for hydroxylation in the prior art. The preparation method comprises the following steps: strain breeding, wherein a strain of ochratoxin or rhizopus nigricans is inoculated to a corresponding seed medium for cultivation; substrate emulsification, wherein a substrate selected from one of 17-alpha hydroxyl progesterone, 4-androstenedione or 16,17-alpha epoxy progesterone is subjected to emulsification treatment under the action of a surfactant; fermented cultivation, wherein the ochratoxin or rhizopus nigricans is inoculated to a fermented medium to be cultivated for a period of time, and then one of emulsion liquids of sterilized 17-alpha hydroxyl progesterone, 4-androstenedione or 16,17-alpha epoxy progesterone is added for performing continuous fermented cultivation; extracting a finished product. The method has the advantages of high conversion rate, little pollution, environmental protection, low pressure and the like.

The invention relates to a one-step rapid detection method of ochratoxin A by using an electrochemical aptamer sensor, comprising the following steps: (1)polishing the surface of a bare gold electrode; (2)modifying the electrochemical aptamer sensor; (3) detecting an ochratoxin A standard concentration solution, and establishing a standard curve; and (4) carrying out quantitative determination on an actual sample solution containing ochratoxin A. According to the invention, the sensitivity of detecting ochratoxin is raised, the minimum detectable amount of ochratoxin A is 0.01 pg/mL, the cost of detecting the target toxin is greatly reduced, the operation is simple and convenient, and the one-step detection of ochratoxin A can be realized in 5-10 min.

The present invention relates to amidase enzymes and a feed or food additive comprising the amidase enzyme capable of degrading ochratoxin.

The invention relates to the technical field of separation medium preparation, in particular to a preparation method and application of an ochratoxin metal organic skeleton-molecular imprinting compounded separation medium. The ochratoxin metal organic skeleton-molecular imprinting compounded separation medium is taken as a filing material for a solid phase extraction column for being applied in selective enrichment and separation of the ochratoxin in complex matrix. A imprinted polymer takes a structural analogue of the ochratoxin as an alternative template, and a metal organic skeleton is combined with the imprinted polymer to prepare the ochratoxin metal organic skeleton-molecular imprinting compounded separation medium. The separation medium prepared by the invention has the porosity characteristic of the metal organic skeleton and the molecular recognition ability of the molecular imprinting polymer to a target molecule, can selectively enrich and isolate the ochratoxin, is applied to the sample pretreatment of the complex matrix to achieve better purification, enrichment effect, and has broad application prospects.

The invention relates to a veterinary medicine composition for preventing and treating mycotoxin pathopoiesia. The veterinary medicine composition is prepared from the follow ingredients in parts by weight: 5 to 25 parts of mildew preventive, 5 to 25 parts of sodium humate, 5 to 25 parts of montmorillonite, 5 to 25 parts of rheum officinale, 5 to 25 parts of scutellaria baicalensis, 5 to 25 parts of coptis chinensis, 5 to 15 parts of liquorice and 5 to 25 parts of radix isatidis. The veterinary medicine composition has the advantages that the specific adsorption capacity is realized, various mycotoxins can be directionally and effectively eliminated, the veterinary medicine composition effectively aims at zearalenone, vomitoxin, aflatoxin, ochratoxin, fumitremorgin, T-2 toxin and the like, the detoxification capability of the liver per se on the toxin can be improved, and the toxin injury to animal bodies is eliminated.

The invention discloses a photonic crystal modified microsphere for detecting OTA (Ochratoxins) based on DNAzyme-aptamer chemiluminescence and the application thereof. The photonic crystal modified microsphere is characterized in that the surface of the photonic crystal microsphere is sequentially subjected to hydroxylation modification, amination modification and carboxylation modification; then the DNA chain and the A1 are fixed to the hydroxylation modified microsphere surface. According to the photonic crystal modified microsphere for detecting the OTA based on the DNAzyme-aptamer chemiluminescence, the photonic crystal microsphere is treated as a fungaltoxin probe vector; an aptamer technology is used as the reaction basis; a G-quadruplet structure formed by Hemin and the DNA chain is treated as a bio-enzyme catalyzing luminol system; on that basis, the chemiluminescence is performed; a chemiluminescence signal of single photonic crystal microsphere is subjected to high throughput test through a multifunctional microplate reader. Therefore, a method which is high in sensitivity, high in throughput, low in cost, fast to detect, and high in specificity is created for building the OTA.

The invention discloses an ochratoxin detoxification enzyme, an encoding gene, a recombinant vector and an application thereof, and belongs to the technical field of enzyme engineering. The inventionprovides the ochratoxin detoxification enzyme and the encoding gene thereof, and also provides the optimum pH of the ochratoxin detoxification enzyme and the application of the ochratoxin detoxification enzyme in degrading ochratoxin and ochratoxin biological detoxification in food. When ochratoxin A is processed by the ochratoxin detoxification enzyme at a temperature of 37 DEG C and at the pH of7.3 for 2min in a buffer system, the degradation rate of ochratoxin A reaches 100%, and the degradation efficiency is unprecedented in the field.

The invention provides amidase and a coding gene, recombinant carrier, recombinant strain and application thereof, and belongs to the technical field of enzyme engineering. The invention provides theamidase and the coding gene thereof, and also provides appropriate pH of the amidase and an application of the amidase to hydrolysis of ochratoxin and detoxifying of ochratoxin of foods. Under the room temperature condition, the amidase is used for treating ochratoxin A in a buffer system of which the pH is 7.3 for 2 minutes, the degradation rate of the ochratoxin A achieves 100%, and the degradation efficiency is unprecedented in the field.

The invention discloses a preparation method for an organic/inorganic hybrid quartz capillary monolithic column modified by single-chain DNA nucleic acid aptamer. The preparation method is performed successively through the following steps: (1) performing activation processing on the internal surface of a hollow quartz capillary; (2) weighing CTAB (hexadecyltrimethylammonium bromide) and putting into a centrifugation tube, respectively adding TEOS (tetraethyl orthosilicate), APTES (3-aminopropyltriethoxysilane), anhydrous ethanol and the like, and performing multi-step processing, so as to obtain an organic/inorganic hybrid monolithic column; (3) subsequently using methanol and pure water for flushing; (4) activating, reacting at room temperature for a period, and using a PBS (phosphate buffer saline) for repeated flushing; and (5) injecting an ochratoxin A aptamer solution into the organic/inorganic hybrid monolithic column, and performing circular reaction at room temperature and storing at a low temperature, so as to obtain the organic/inorganic hybrid quartz capillary monolithic column modified by single-chain DNA nucleic acid aptamer. The product is applied to high-selectivity separation and enrichment of trace quantity or ultra trace quantity of ochratoxin, aflatoxin and other substances in complex biological samples and food.

The invention discloses a mycotoxin adsorption degradation agent for pig feed and a preparation method thereof. The mycotoxin adsorption degradation agent is prepared from the following components inparts by weight: 15 to 25 parts of activated lactic acid bacteria, 15 to 25 parts of bacillus subtilis, 20 to 40 parts of saccharomycetes, 20 to 30 parts of lactic acid bacterium cell wall peptidoglycan, 2 to 6 parts of an aflatoxin degeneration enzyme, 2 to 6 parts of a zearalenone degeneration enzyme, 2 to 6 parts of a vomitoxin degeneration enzyme, 2 to 6 parts of an ochratoxin degeneration enzyme, 2 to 6 parts of a T-2 toxin degeneration enzyme, 100 to 200 parts of hydrated aluminosilicate, 10 to 20 parts of potassium sorbate, 10 to 20 parts of sodium benzoate, 25 to 40 parts of pericarpium citri reticulatae, 25 to 40 parts of fructus crataegi, 25 to 35 parts of cortex cinnamomi and 10 to 25 parts of flos caryophylli. The adsorption degradation agent is subjected to biological adsorption and intestinal degradation, so that mycotoxin in raw materials of the feed and in the feed can be effectively removed and the health and safety of live pigs are ensured.

The invention discloses stable controllable high-quality Pu'er tea and a fermentation production method thereof. The Pu'er tea is fermented Pu'er tea. In dry base mass, the content of tea polyphenol is 10.0 to 15.0 weight percent, the content of theabrownin is 8.0 to 16.0 weight percent, and the content of a water extract is 30.0 to 45.0 weight percent; in the Pu'er tea, the content of aflatoxin B1 is lower than 0.03mu g/kg, the content of fumonisins B1 is lower than 7mu g/kg, the content of fumonisins B2 is lower than 3mu g/kg, the content of fumonisins B3 is lower than 3mu g/kg, the contentof ochratoxin is lower than 0.3mu g/kg, and the content of vomitoxin is lower than 5mu g/kg. According to the fermentation production method disclosed by the invention, a brand-new fermentation production method of the Pu'er tea is established; the production efficiency is high and the obtained fermentation Pu'er tea has the advantages of high quality, good mouth feel, bright soup color, red-brownleaf bottom and controllable content of important components of materials; in addition, the aflatoxin, the fumonisins, the ochratoxin, the vomitoxin and the like are not detected from the Pu'er tea.

The invention discloses a method for improving the degradation effect of ochratoxin A (OTA) in a solution by using electron beam irradiation. The method is characterized in that the OTA in the aqueous solution is subjected to irradiating treatment by adopting electron beams generated by an electron accelerator, and.OH with extremely strong oxidizability, hydrated electrons (a formula shown in the specification) with extremely strong reducibility and H. radicals are produced due to the radiolysis of water after treatment, wherein.OH free radicals can be subjected to addition reaction with benzene rings and unsaturated bonds of OTA, and the Cl element of OTA is easily attacked by the hydrated electrons, so that OTA is dechlorinated; after OTA is dechlorinated, the H. free radicals are rapidly added to groups, so that OTA is degraded. The process conditions for improving the degradation effect are as follows: the pH value of the OTA aqueous solution is adjusted to be 7-10 during irradiation, the initial concentration of the solution is not higher than 200ng/mL, and the degradation rate of OTA is over 98% when the adopted radiation dose is 1-12kGy. The method has the advantages that the operation is simple and convenient, the effect is remarkable, and OTA in the OTA aqueous solution can be effectively degraded.

A multi-analyte column is disclosed. The column may contain at least one unit of resin having ochratoxin specific affinity and, for each unit of resin having ochratoxin specific affinity, the column further contains about 0.95 to 1.05 units of resin containing antibody having specificity for zearalenone, about 1.9 to 2.1 units of resin containing antibody having specificity for aflatoxin and about 2.8 to 3.2 units of resin containing antibody having specificity for fumonisin. One unit of resin is the quantity of resin containing antibody that will bind 50 ng of aflatoxin, 3300 ng of fumonisin, 50 ng of ochratoxin or 1140 ng of zearalenone, respectively.

The invention belongs to the technical field of food detection, and discloses a method for detecting ochratoxin A based on a copper ion fluorescent probe indirect competition method. The method comprises the following steps: forming immune signal markers of carboxylate copper sulfide nano-particle-ochratoxin A monoclonal antibodies; fixing ochratoxin A antigens to a black polystyrene microporous plate, wherein after a sample to be detected is added, the ochratoxin A antigens in the sample to be detected and the ochratoxin A competitively bind to the ochratoxin A monoclonal antibodies on the markers; washing the immune signal markers of the carboxylate copper sulfide nano-particle-ochratoxin A monoclonal antibodies which are not bonded to the ochratoxin A antigens fixed to the black polystyrene microporous plate, dissolving out the copper ions which are marked by immune signals of the carboxylate copper sulfide nano-particle-ochratoxin A monoclonal antibodies bonded to the ochratoxin Aantigens fixed to the black polystyrene microporous plate, and adding a copper ion fluorescent probe to catalyze the copper ions to perform quantitative fluorescence detection. The method disclosed bythe invention is low in cost and easy to operate.

Nucleotide or amino acid sequences that may be used in the detection and/or identification for an ochratoxigenic fungus or in the construction of an atoxigenic strain of an ochratoxigenis fungus. The fungus may be of the genus Aspergillus, species carbonarius, niger, alliaceus, or foetidux. The fungus may also be of the genus Penicillium, species verrucosum.

The invention discloses a method for simultaneously rapidly determining total amount of three kinds of ochratoxin in samples. The method comprises the following steps: adding a mixed solvent of acetonitrile and glacial acetic acid of the same volume into a to-be-determined sample, uniformly mixing, adding a QuEChERS cleaning agent 1, uniformly mixing, performing centrifugal separation, and takingthe supernatant; adding a QuEChERS cleaning agent 2, uniformly mixing, performing centrifugal separation, taking the supernatant, and passing through an organic filter membrane so as to obtain a to-be-determined solution; simultaneously preparing a standard working solution and making a standard curve; and finally, adopting high performance liquid chromatography or high performance liquid chromatography-tandem mass spectrometry, simultaneously detecting ochratoxins A, B and C in the to-be-determined solution, and performing quantitative analysis according to the established standard curve. According to the detection method, the three kinds of ochratoxin in samples can be simultaneously and rapidly determined, and the method is excellent in purification effect, simple and convenient to operate, rapid, low in cost and less in dose of organic reagents, has high sensitivity, accuracy and precision, can be applied to routine testing of the ochratoxin, and has an important application valueand practical significance.

The invention relates to upconversion magnetically-coded microspheres for mycotoxin flux detection and a preparation method thereof. The upconversion magnetically-coded microspheres used for simultaneous detection of various mycotoxins have a diameter of 2-8 microns. By irradiation with near-infrared light of 980 nm, different upconversion ion coded microspheres can emit different colors of visible light, each color represents a substance to be tested, so the microspheres can be used for simultaneous detection of various mycotoxins. Red, green and blue upconversion coded microspheres are separately linked with complete antigens of ochratoxin, zearalenone and aflatoxin to be used as models convenient for later detection experiments. However, in this method, the correspondence between colorcoding and mycotoxins is not unique, and the corresponding sequence can be changed according to the experimental needs. The flux, sensitive and rapid detection of various mycotoxins in the liquid phase environment is conducted by upconversion coded microspheres linked with mycotoxin complete antigens in combination with an indirect immune competitive reaction.

The invention relates to a method and kit for detecting ochratoxin A. The method comprises A, carrying out hybridization on an anti-ochratoxin A aptamer (Apt) and a single chain signal probe DNA (Sp) capable of being hybridized with the anti-ochratoxin A aptamer (Apt) so that a hybrid chain is formed, B, contacting the hybrid chain and a sample to be detected, wherein when the sample to be detected contains ochratoxin A, the hybrid chain and ochratoxin A undergo a reaction to produce the single chain signal probe DNA, C, carrying out DNA amplification so that the hybrid chain forms a double-stranded DNA, chopping the double-stranded DNA through an excision enzyme and leaving on the single chain signal probe DNA and D, detecting system fluorescence intensity in a silver ion reduction detection system and determining content of the mycotoxin ochratoxin A. The detection method and kit can eliminate interference and improve detection sensitivity and precision of ochratoxin A.

The invention provides a fluorescent silver nanometer cluster with stable nucleic acid, and a preparation method and application thereof to toxin detection. The method comprises the following steps ofdesigning two kinds of silver nanometer clusters (AgNCs) with stable nucleic acid, wherein a sequence connected onto the AgNCs with stable DNA can be a toxin aptamer sequence; when the sequence connected onto the AgNCs with stable DNA is an ochratoxin (OTA) aptamer, under the OTA existence condition, the action force of the OTA and the aptamer at one end of the aptamer with stable DNA is greaterthan the action force between the single-chain DNA and the WS2, so that the AgNCs is disengaged from WS2; the fluorescence is further recovered. In addition, when the aptamer containing two kinds of different toxins in the solution is connected with DNA-stable AgNCs of different light, two kinds of detection targets are added at the same time; the simultaneous detection of the two kinds of toxin can be realized. The method has the advantages of high selectivity, high sensitivity and low cost; the OTA and AFB1 detection lower limit is respectively 0.5ng/mL and 0.5 ng/mL.