9 results about "Ochratoxins" patented technology

The application discloses application of an enzyme in degrading ochratoxin A. The amino acid sequence of the enzyme comprises: (1) a sequence shown in SEQ ID NO. 1, or (2) an amino acid sequence obtained by substituting, deleting or adding one or at least two amino acid residues from the sequence in (1) and being identical or similar in function to the sequence in (1), or (3) an amino acid sequence having at least 90% sequence homology with the sequence in (1) or (2) and being identical or similar in function to the sequence in (1). The application first discovers that an enzyme derived from Thermomonas fusca has the function of degrading ochratoxin A, and catalyzes ochratoxin A to degrade into non-toxic substances, so as to be expected to be applied in degrading ochratoxin A, and the degradation rate is more than 90%, and the application has substantial application value and great significance in the aspect of biological detoxification of food and feed.

The application provides application of an amide bond hydrolase in degrading ochratoxin A, and the amide bond hydrolase has any one of the amino acid sequences shown in (I), (II) or (III): (I) an amino acid sequence shown in SEQ ID NO:1; (II) an amino acid sequence with homology of greater than or equal to 90% to the amino acid sequence shown in SEQ ID NO:1, and an amino acid sequence with the same function or similar function as the sequence shown in (I); (III) an amino acid sequence obtained by modifying, substituting, deleting or adding at least one amino acid of the amino acid sequence shown in SEQ ID NO:1. The amide bond hydrolase has the function of degrading ochratoxin A, and the degradation rate is greater than or equal to 99.09%, and can be used as a detoxicant of ochratoxin A, and has important application value in food processing and food safety guarantee.

The application discloses application of an amide hydrolase OtaH in degrading ochratoxin A, and relates to the technical field of enzyme engineering and food safety biotechnology. The amino acid sequence of the amide hydrolase OtaH is shown as SEQ ID NO. 2, the coding gene sequence of the amide hydrolase OtaH is shown as SEQ ID NO. 1, and the application provides application of the amide hydrolase OtaH in degrading ochratoxin A. The amide hydrolase OtaH can specifically hydrolyze the amide bond of ochratoxin A (OTA) to generate almost non-toxic ochratoxin alpha, the enzyme has high catalytic efficiency, 5.0 mu g / mL of the enzyme amount can completely degrade 12.4 mu M OTA in 5 minutes, and more than 50% activity is still maintained after incubation at 65 DEG C for 1 hour, thereby showing excellent thermal stability. The amide hydrolase OtaH can be widely used in detoxification treatment of OTA in feed and food, and the harm of OTA to human beings and animals is reduced.

The present application relates to the technical field of experimental animal pathological model modeling, and more particularly to a glandular gastritis modeling agent, a preparation method and application thereof.The present application relates to a glandular gastritis modeling agent, which comprises a ZnO-OTA core and a mesoporous silica shell layer coated on the surface of the ZnO-OTA core; the ZnO-OTA core comprises amino zinc oxide nanoparticles and ochratoxin A.In the present application, the amino zinc oxide nanoparticles and ochratoxin A are combined through electrostatic adsorption, and then coated with mesoporous silica to form a core-shell structure; when applied to the construction of a broiler glandular gastritis pathological model, a stable and high-pathology-compliance glandular gastritis model can be established, the required time is short, the modeling effect is good, and a stable and high-pathology-compliance glandular gastritis model can be established within 21 days.

This invention proposes an ochratoxin α nucleic acid aptamer and its application in fluorescence in situ imaging. Specific nucleic acid aptamers with high affinity for ochratoxin α were screened and obtained using SELEX technology, and a fluorescence in situ imaging technique based on primer substitution amplification signal was constructed. This sensor uses the target ochratoxin α nucleic acid aptamer as a recognition element, and incorporates primer substitution amplification technology to amplify the detection signal, thereby achieving in situ detection of ochratoxin α. It possesses the advantages of high specificity and ease of operation, providing a new direction for the application of aptamers in fluorescence in situ imaging.

The application discloses a method for synchronous detection of multiple mycotoxins. After crushing the sample to be detected, the sample is pretreated by using a hierarchical QuEChERS extraction method and / or a solid-phase extraction method according to the characteristics of the matrix to prepare a sample extract. A mixed control standard working solution containing aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, citrinin, ochratoxin A and zearalenone is prepared, and the seven mycotoxins are detected by using ultra-high performance liquid chromatography-tandem mass spectrometry. Qualitative and quantitative analysis is realized by alternately scanning positive and negative ions and multi-reaction monitoring. The method can be adapted to the need for mycotoxin control of traditional Chinese medicine decoction pieces, does not need an immunoaffinity column, has high pretreatment efficiency, low detection cost and high throughput, can realize synchronous and accurate detection of multiple toxins, and meets the demand for rapid screening of large batches of traditional Chinese medicine decoction pieces.

The application discloses a method for detecting ochratoxin A by using a competitive colorimetric fluorescent dual-mode immunosensor, and the method uses AuAg NCs-S,P-g-C3N4 nanocomposites as a label of the competitive colorimetric fluorescent dual-mode immunosensor; the S,P-g-C3N4 has the advantages of a large specific surface area and good biocompatibility; the AuAg NCs have an aggregation luminescence effect after being combined with the S,P-g-C3N4 through Au-S bonds, the fluorescence of the AuAg NCs is enhanced, the fluorescence signal is amplified, and the sensitivity of the sensor is improved; the loading of the AuAg NCs greatly enhances the enzyme-like activity of the S,P-g-C3N4; the nanocomposites are used to catalyze TMB into blue oxTMB, so that visual detection is realized. The two signals are mutually verified, so that the sensor is more sensitive, and the method has the characteristics of simple operation, fast reaction speed, low cost, low detection limit and wide linear range.

The application relates to the technical field of food detection, in particular to high-stability perovskite fluorescent material preparation and application thereof in mycotoxin detection. High-stability perovskite fluorescent material is prepared, and a MOF-CsPbBr3 fluorescent probe is prepared through the high-stability perovskite fluorescent material, the MOF-CsPbBr3 fluorescent probe is applied in mycotoxin detection in edible oil, and the mycotoxin is ochratoxin A. The high-stability perovskite fluorescent material preparation and application thereof in mycotoxin detection provided by the application are as follows: MOF material is prepared under room temperature conditions, then a CsPbBr3 fluorescent material coated with the MOF material is prepared by adopting a ligand-assisted precipitation method, and is recorded as MOF-CsPbBr3; and the MOF-CsPbBr3 is used for quantitative detection of ochratoxin A (OTA) in edible oil, and exhibits good detection sensitivity, and has a good development prospect in mycotoxin detection.

This invention relates to a non-targeted identification method for mycotoxin metabolites in wine based on mass spectrometry coupled with chromatography, specifically for the detection and identification of ochratoxin A and its metabolites in wine. Through sample pretreatment, chromatographic separation, mass spectrometry detection, data screening, and fragment ion confirmation of wine samples, non-targeted detection of ochratoxin A and its metabolites can be achieved without relying on standards. In the sample pretreatment stage, the target analyte is extracted using acidified acetonitrile solution, and impurities are removed by salting out and centrifugation. Separation of ochratoxin A and its metabolites is achieved by coupling high-performance liquid chromatography with time-of-flight mass spectrometry using a reversed-phase C18 column for gradient elution. The mass spectrometry analysis uses full-scan mode and alternates between positive and negative ion modes to ensure high resolution and high sensitivity, making it suitable for the detection of mycotoxins in wine and other foods, meeting food safety regulatory requirements.