200 results about "Ochratoxins" patented technology

The invention relates to an electrochemical lateral-flow immunity quantitative test paper sensor based on microclearance array electrode, and a method thereof used for detecting biological toxins such as ochratoxin A and fumonisin B1. The sensor includes two parts, namely, immune chromatographic analysis test paper strip and electrochemical detecting part. The fast detecting test paper sensor has strong specificity, can realize quantitative detection, can be used at the temperature between 4 and 40 DEG C, and the result can be observed after ten minutes, thus being suitable for units or individuals to quickly detect ochratoxin A and fumonisin B1 in animal derived food samples, and being expected to become an effective technical means for the field screening of ochratoxin A and fumonisin B1 in food and feed samples.

A microorganism for the biological inactivation or detoxification of mycotoxins, in particular ochratoxins, which is selected from bacteria and/or yeasts, which cleaves the phenylalanine group of the mycotoxins, in particular ochratoxins, as well as a method for biologically inactivating or detoxifying mycotoxins, in particular ochratoxins, in food products and animal feeds by the aid of a microorganism, and the use of the microorganism(s).

The invention relates to a one-step rapid detection method of ochratoxin A by using an electrochemical aptamer sensor, comprising the following steps: (1)polishing the surface of a bare gold electrode; (2)modifying the electrochemical aptamer sensor; (3) detecting an ochratoxin A standard concentration solution, and establishing a standard curve; and (4) carrying out quantitative determination on an actual sample solution containing ochratoxin A. According to the invention, the sensitivity of detecting ochratoxin is raised, the minimum detectable amount of ochratoxin A is 0.01 pg/mL, the cost of detecting the target toxin is greatly reduced, the operation is simple and convenient, and the one-step detection of ochratoxin A can be realized in 5-10 min.

The invention discloses a composite affinity column for purifying 3-acetyl deoxynivalenol, aflatoxin, ochratoxin A and zearalenone, a preparation method and an application thereof. According to the invention, by employing 4% of cylindrical agarose gel as a solid phase carrier, agarose gel and an antibody are coupled to form an immunoadsorbent, and filling in a column to prepare the immunization affinity column. When a sample containing 3-acetyl deoxynivalenol, aflatoxin, ochratoxin A and zearalenone passes through the immunization affinity column, 3-acetyl deoxynivalenol, aflatoxin, ochratoxin A and zearalenone can be specifically adsorbed by the immunoadsorbent, other impurities flows out of the immunization affinity column, then methanol is used for eluting 3-acetyl deoxynivalenol, aflatoxin, ochratoxin A and zearalenone from the column, so that the sample can be better purified.

The invention relates to the field of biotechnology, in particular to an ochratoxin degrading enzyme, a coding gene, a recombinant vector, a cell, an additive and application thereof. The invention more specifically relates to the ochratoxin degrading enzyme with a sequence shown as SEQ ID NO:2 or a mutant thereof, a coding gene of the enzyme, a vector and a cell containing the coding gene, and anadditive containing the enzyme and/or cell and/or a fermentation product thereof, application of the enzyme, the coding gene, the vector, the cell or additive in degradation of ochratoxin and/or other fungal toxins, as well as a method for degradation of ochratoxin/or other fungal toxins. The ochratoxin degrading enzyme provided by the invention is environment-friendly, can efficiently degrade ochratoxin in a short time, and does not generate any harmful by-product. The enzyme can tolerate high temperature catalysis up to 80DEG C, also has low pH value requirement, has good stability, and achieve degradation of fumonisins and T2 toxin to a certain degree.

Relating to the field of removal of fungal toxins, the invention discloses a compound enzyme, an additive, application thereof and a method for removal of fungal toxins. Specifically, the invention relates to a compound enzyme, which contains amidase and esterase and can remove fungal toxins, especially fumonisins, ochratoxins and T2 toxin, additives containing the compound enzyme and applicationthereof in removal of fungal toxins, especially fumonisins, ochratoxins and T2 toxin, and a method for removal of fungal toxins. According to the technical scheme, combined use of the amidase and esterase can achieve simultaneous removal of ochratoxin A, fumonisins and T2 toxin, the removal efficiency is greatly improved than single use of one enzyme, and vomitoxins, aflatoxins and zearalenone toxin can be removed to certain degree.

The invention discloses a POSS (polyhedral oligomeric silsesquioxane)-based organic-inorganic hybrid silica gel monolithic column applied to ochratoxin specific recognition and a preparation method thereof. The POSS-based organic-inorganic hybrid silica gel monolithic column is characterized in that the hybrid silica gel monolithic column prepared by nucleophilic reaction between a polyhedral oligomeric silsesquioxane (POSS) crosslinking agent and polyamine monomer is utilized as a matrix, the matrix has a high-crosslinking 3D skeleton, the surface of the matrix contains rich amino, the matrixcan load anti-ochratoxin aptamer surface modified gold nano particles in high density, and the monolithic column is prepared by nano gold amino bonding and self assembling. The prepared POSS-based monolithic column matrix disclosed by the invention has the characteristics of high-crosslinking structure and multiple acting sites and can efficiently load the nano gold particles; thus, the aptamer with specific recognition on ochratoxin A can be loaded and fixed to the POSS-based monolithic column in high density, and high-efficiency specific recognition separation on the ochratoxin A is achieved.

The invention relates to a test paper card for synchronously detecting ochratoxin, vomitoxin and T-2 toxin, a preparation method and a detection method, and belongs to the field of immunological detection. The test paper card comprises a card shell and a test paper strip, wherein the test paper strip comprises a base plate, as well as an absorbent pad, a detection pad, a conjugate pad and a samplepad which are sequentially lapped and stuck to the base plate; the detection pad is an NC film provided with a quality control line, a detection line T1, a detection line T2 and a detection line T3;the quality control line coats a goat anti-mouse secondary antibody; the detection line T1 coats OTA-BSA; the detection line T2 coats DON-BSA; the detection line T3 coats T-2-BSA; the conjugate pad isa glass cellulose film, labeled by embedding time-resolved fluorescent microspheres, of an ochratoxin monoclonal antibody, a vomitoxin monoclonal antibody and a T-2 toxin monoclonal antibody; the sample pad is a dried glass cellulose film soaked by sample pad treating fluid. The invention provides a novel method for systematic, convenient and normalized quantitative synchronous detection of at least two fungaltoxins. The novel method has the advantages of good stability and high sensitivity.

A method of rendering harmless mycotoxins contaminating food, animal feed and assisting infection of plant hosts by microbial parasites is proposed. The method comprises binding mycotoxins by a novel adsorbent, consisting partially or in full of the biomass of filamentous fungi or isolated fungal biomass components, such as chitin, chitozan and hyrdophobins, or fungal biomass enriched in its capacity to bind said mycotoxins using fungal species and strain selection, genetic engineering of fungi, modification of fungal fermentation conditions and downstream physical and chemical treatment of fungal biomass, such as milling or micronization in a dry state. The resulting adsorbent can bind a wide range of mycotoxins, including the ones difficult to bind (Ochratoxin, T-2, DON, NIV) using a current generation of mycotoxin adsorbents based on clay, resins, yeast and bacterial biomass. The adsorbent can be used as an animal feed additive, functional food additive and agricultural fungistatic/fungicide.

A multi-analyte column is disclosed. The column may contain at least one unit of resin having ochratoxin specific affinity and, for each unit of resin having ochratoxin specific affinity, the column further contains about 0.95 to 1.05 units of resin containing antibody having specificity for zearalenone, about 1.9 to 2.1 units of resin containing antibody having specificity for aflatoxin and about 4.7 to 5.3 units of resin containing antibody having specificity for DON. One unit of resin is the quantity of resin containing antibody that will bind 50 ng of aflatoxin, 500 ng of DON, 50 ng of ochratoxin or 1140 ng of zearalenone, respectively.

The invention belongs to the technical field of electrochemical sensing, and relates to a preparation method of a ratio electrochemical aptamer sensor for simultaneously detecting AFB1 and OTA. The sensor is prepared by assembling AQ-hDNA, Fc-Apt1 and MB-Apt2 on a gold electrode in sequence, the combination of a target object and an aptamer causes Fc-Apt1 and MB-Apt2 to be stripped from a surfaceof the electrode such that ratio signals IFc/IAQ and IMB/IAQ are reduced, and the purposes of respectively quantifying and detecting AFB1 and OTA are achieved; the linear detection range of the sensoron AFB1 is 10 pg/mL-3 ng/mL, and the detection limit is 3.3 pg/mL; the detection linear range on OTA is 30 pg/mL-10 ng/mL, and the detection limit is 10.0 pg/mL; two substances are detected at the same time, and the method has the characteristics of high sensitivity, good selectivity, high accuracy and the like.

The present invention relates to a new method for rapidly detecting ochratoxin A through a molecular beacon probe. According to the present invention, based on the DNA key site for specifically recognizing OTA, a molecular beacon having the stem comprising sixes complementary bases and the ring having 8 bases is designed, wherein the sequence of the molecular beacon is 5'-FAM-CCGGGTCCACCCACACCCGG-DABCYL-3'; and the probe is stable and is easy to synthesize, is used for sample analysis, has the simple operation, can complete the detection within 20 min, further has advantages of good accuracy and high sensitivity, and can be used for the rapid screening of the ochratoxin A in malt, beer, and other samples.

The invention relates to an irradiation degradation treatment method for fumitremorgin and ochratoxin. The irradiation degradation treatment method comprises the following steps: (1) preparing standard solutions of standard products of the fumitremorgin FB1 and ochratoxin OTA; carrying out irradiation treatment on the standard solutions in an irradiation range of 0kGy-200kGy; determining the content of the FB1 and the OTA by adopting a liquid chromatogram-tandem mass spectrometry; (2) pre-treating a poisoned sample after irradiating in an irradiation range of 0kGy-9kGy; determining degradation effects on the FB1 and the OTA by adopting the liquid chromatogram-tandem mass spectrometry; and (3) selecting the FB1 and OTA standard solutions with the highest concentration as a sample and carrying out detection and analysis on a degraded product by using the liquid chromatogram-tandem mass spectrometry after irradiating in the irradiation range of 0kGy-200kGy. According to the irradiation degradation treatment method for the fumitremorgin and the ochratoxin, researches and applications of FB1 and OTA degraded products which are lack of irradiation degradation are solved; the degradation effect is good and the environment-friendly detoxification treatment of mildewed agricultural product wastes is realized.

The invention discloses a method for determining the content of ochratoxin A in juice. The method comprises the following steps: pretreating a sample by adopting a simple liquid-liquid extraction and purification step; scanning with parameters including optimized scanning wavelengths, scanning intervals and the like, and acquiring three-dimensional fluorescent data of a standard product and the sample; carrying out mathematic separation treatment on the obtained data by adopting a parallel factor analyzing method (PARAFAC); establishing a correction model by using the standard product with the known concentration and by combining mathematic separation with chemical and physical separation; and predicating a component to be detected under the conditions that unknown and uncorrected background interferences are included and spectrums are seriously overlapped. The method is simple and rapid, and has the high sensitivity; and the content of ochratoxin in the juice can be determined under the unknown background interferences. The method belongs to the field of food safety.

The invention discloses an ochratoxin detoxification enzyme, an encoding gene, a recombinant vector and an application thereof, and belongs to the technical field of enzyme engineering. The inventionprovides the ochratoxin detoxification enzyme and the encoding gene thereof, and also provides the optimum pH of the ochratoxin detoxification enzyme and the application of the ochratoxin detoxification enzyme in degrading ochratoxin and ochratoxin biological detoxification in food. When ochratoxin A is processed by the ochratoxin detoxification enzyme at a temperature of 37 DEG C and at the pH of7.3 for 2min in a buffer system, the degradation rate of ochratoxin A reaches 100%, and the degradation efficiency is unprecedented in the field.

The invention provides amidase and a coding gene, recombinant carrier, recombinant strain and application thereof, and belongs to the technical field of enzyme engineering. The invention provides theamidase and the coding gene thereof, and also provides appropriate pH of the amidase and an application of the amidase to hydrolysis of ochratoxin and detoxifying of ochratoxin of foods. Under the room temperature condition, the amidase is used for treating ochratoxin A in a buffer system of which the pH is 7.3 for 2 minutes, the degradation rate of the ochratoxin A achieves 100%, and the degradation efficiency is unprecedented in the field.

The invention discloses an aptamer of ochratoxin A and a nucleotide sequence of complementary DNA of the aptamer, further discloses a method for quantitatively detecting ochratoxin A, and belongs to the field of quantitative detection of ochratoxin A. The method includes the following steps that 1, an aptamer biosensor is prepared; 2, ochratoxin A in a sample to be detected is extracted to obtain sample extraction liquor, the sample extraction liquor is added into the aptamer biosensor and evenly mixed, and incubation is performed; 3, supernatant liquor is separated, and an excessive sucrose solution is added for a reaction; 4, quantitative detection is performed through a glucometer. The method for quantitatively detecting ochratoxin A in food or feed by combining the aptamer biosensor with the glucometer is good in specificity and repeatability, high in sensitivity, fast to implement and low in cost and provides a new means for quantitatively detecting ochratoxin A.

The invention relates to a composite type mycotoxin adsorbent comprising 20-80% of high-purity montmorillonite, 0-40% of surface-modified montmorillonite and 0-40% of carbon-coated montmorillonite. The surface-modified montmorillonite is prepared by modification with a cationic or non-ionic compound as a surface modifier; the carbon-coated montmorillonite is obtained by mixing evenly a carbon source material acidified thick slurry with an acidified montmorillonite thick slurry and then carrying out high temperature calcination activation. The high-purity montmorillonite is mainly used for adsorption of aflatoxin; the surface-modified montmorillonite is mainly used for adsorption of zearalenone and ochratoxin; the carbon-coated montmorillonite is mainly used for adsorption of vomitoxin; and through synergism effects after compositing of various components, the product is allowed to have good adsorption capacity on various mycotoxins.

The invention discloses an organic polymerized monolithic column capable of specially recognizing ochratoxin, and a preparation method of the organic polymerized monolithic column. According to the monolithic column, a polymerized monolithic column fixed phase having a branched structure of an epoxy group is firstly formed by polymerizing an epoxy cyclosiloxane acrylate monomer and an acrylate cross-linking agent, then post-column ring-opening addition is carried out, and an aptamer capable of specially recognizing the ochratoxin is prepared by covalent bonding. The aptamer and an epoxy group, distributed on the surface of the epoxy cyclosiloxane polymerized monolithic column matrix fixed phase in a branching way, are subjected to further ring-opening addition, so that condensation coupling is realized; the prepared polymerized monolithic column matrix fixed phase has a cyclosiloxane octatomic ring-shaped structure and the epoxy group distributed in a branching way, thus being stable in structure; the organic polymerized monolithic column is more in sites acting with 5'-amino aptamer and small in steric hindrance, and can realize efficient bonding with ochratoxin A aptamer, thus being suitable for the specific recognition of the ochratoxin A.

The invention discloses a kit for synchronously detecting six fungal toxins. The six toxins are aflatoxin, ochratoxin, patulin, trichothecene mycotoxin, fumonisins and zearalenone; and based on a multiple PCR detection method, the kit comprises a first primer pair, a second primer pair, a third primer pair, a fourth primer pair, a fifth primer pair, a sixth primer pair and an internal standard primer pair which can perform PCR reaction in the same reaction tube. The detection system directly targets a toxin synthesis essential gene and has high specificity; an internal reference gene is amplified synchronously, positive, negative and blank control are set simultaneously, and false negative and false positive are effectively avoided; and multiplication of PCR index guarantees the sensitivity of the method.

The invention discloses a method for detecting ochratoxin A in vegetables and fruits. The method includes the steps of firstly, adopting acetonitrile and tetrahydrofuran as extraction agents, performing extraction by a quick ultrasonic extraction method, centrifuging quickly and extracting supernatant; secondly, purifying the supernatant by immunoaffinity columns of the ochratoxin A, performing volume metering, and filtering via a 0.22-micrometer organic phase filter membrane to obtain liquid to be detected. The method for detecting the ochratoxin A in the vegetables and the fruits has the advantages that by the aid of the step one, extracting efficiency is improved greatly, matrix effects of a sample are reduced, and high applicability is achieved; the second step is simple to operate and good in purifying effect; the method is applicable to detecting the ochratoxin A in various kinds of vegetables and fruits, is low in chromatographic technique cost and good in separation effect and chromatographic recurrence and can be used for rapid quantitative detection of the ochratoxin A in trace groups in a complex matrix.