59 results about "Ochratoxins" patented technology

The invention discloses a colorimetric aptamer sensor and application thereof, and belongs to the technical field of biological detection. The colorimetric aptamer sensor comprises Cas12a protein, HP, crRNA, dsDNA (double-stranded deoxyribonucleic acid) and a colorimetric probe. The colorimetric biosensor is applied to detection of the ochratoxin A, the content of the ochratoxin A is detected through the colorimetric biosensor, and detection and analysis of a target object can be achieved; cas12a aptamer inhibitors are developed as molecular switches for biosensors, which mediate target recognition through engineering modification of HP hairpin sequences. A new thought for improving the mycotoxin detection sensitivity is explored, and good detection potential is expected to be shown in the field of food safety.

The invention discloses bacillus subtilis, a complex microbial inoculant and application of the complex microbial inoculant to degradation of mycotoxin, and belongs to the field of microorganisms and fermentation engineering. According to the invention, a strain of bacillus subtilis BLYC-1937 with the activity of degrading mycotoxin is obtained through screening, and the bacillus subtilis BLYC-1937 has a good degradation effect on pollution of single toxin of T-2 toxin, vomitoxin, fumonisins B1, aflatoxin B1 and ochratoxin A or combined pollution of multiple toxins; the strain has good antagonism on fusarium oxysporum capable of producing T-2 toxin and aspergillus ochraceus capable of producing ochratoxin A, and pollution of T-2 toxin and ochratoxin A can be reduced from the source.

The invention relates to the technical field of microorganisms, and discloses efficient detoxification of a strain of pseudoxanthomonas to ochratoxin A and application of the strain of pseudoxanthomonas to the ochratoxin A. A strain of OTA efficient detoxification strain pseudoxanthomonas X-22 is screened out, and the pseudoxanthomonas X-22 can reduce 50 mu g / mL of OTA (10000 times of the national standard) to 0 within 70 min. The strain is mainly used for performing biological detoxification on OTA through intracellular enzyme, and a detoxification product is OTalpha with extremely low toxicity. The optimal growth conditions of the pseudoxanthomonas X-22 are as follows: 1.5 wt% of glucose is added into a basic culture medium as a carbon source, 1.5 wt% of yeast extract is added into the basic culture medium as a nitrogen source, the pH value of the culture medium is adjusted to 7.5, and the culture temperature is 37 DEG C. The OTA detoxification strain pseudoxanthomonas X-22 is applied to fermentation detoxification of polluted corn and oat, the detoxification rates of the corn and the oat are 61.29% and 97.26% respectively, OTA detoxification strain resources are enriched, and a theoretical basis is provided for application of a biological detoxification technology in the corn and the oat.

The invention relates to the technical field of traditional Chinese medicines, in particular to a preparation method of fermented pinellia tuber, which comprises the following specific steps: S1, crushing rhizoma pinelliae preparata and medicated leaven into fine powder; s2, preparing ginger juice; s3, dissolving alum in a proper amount of water, adding the ginger juice, uniformly mixing, and uniformly mixing with the rhizoma pinelliae preparata, the medicated leaven, the flour and the raw bran which are crushed into fine powder; s4, knocking out an ochratoxin synthetic gene (otaA) from the aspergillus oryzae CICC 40186 through CRISPR-Cas9, and transferring the ochratoxin synthetic gene (otaA) into a lactic dehydrogenase gene (ldhL) of lactobacillus plantarum; s5, preparing a compound bacterium suspension; s6, placing the inoculated material in a solid state fermentation reactor; s7, vacuum freeze drying and variable-temperature drying are combined; the consistency of components of different batches of fermented pinellia can be guaranteed, the risk of infectious microbe contamination can be avoided, ochratoxin can be completely eradicated, the fermentation period can be greatly shortened, the overall production efficiency can be improved, the content of the key active component gamma-aminobutyric acid can be greatly improved, and the stability between batches is good.

The application discloses application of an enzyme in degrading ochratoxin A. The amino acid sequence of the enzyme comprises: (1) a sequence shown in SEQ ID NO. 1, or (2) an amino acid sequence obtained by substituting, deleting or adding one or at least two amino acid residues from the sequence in (1) and being identical or similar in function to the sequence in (1), or (3) an amino acid sequence having at least 90% sequence homology with the sequence in (1) or (2) and being identical or similar in function to the sequence in (1). The application first discovers that an enzyme derived from Thermomonas fusca has the function of degrading ochratoxin A, and catalyzes ochratoxin A to degrade into non-toxic substances, so as to be expected to be applied in degrading ochratoxin A, and the degradation rate is more than 90%, and the application has substantial application value and great significance in the aspect of biological detoxification of food and feed.

This invention concerns a method of using mycotoxin binder in broiler chicken feed to reduce the immunotoxicity of aflatoxin B1 and ochratoxin A. This method is implemented by mixing mycotoxin binder into broiler chicken feed contaminated with aflatoxin B1 and ochratoxin A at a certain dose, then providing the feed to broiler chickens during the rearing period. The application of this method is able to reduce the toxic impact of mixed mycotoxins on the immune system of broiler chickens by improving the immune response and protection against oxidative stress. This method provides benefits in the form of improving the health and endurance of broiler chickens and reducing economic losses in the poultry industry due to decreased performance and health of livestock.

This invention relates to a formulation and method of application of a multicomponent mycotoxin detoxifier for poultry feed, particularly female broiler chickens, which aims to provide protection to the Bursa Fabricius due to exposure to mycotoxins in the form of aflatoxin and ochratoxin. The mycotoxin detoxifier formulation according to this invention consists of an inorganic mycotoxin binder in the form of bentonite and / or zeolite, an organic mycotoxin binder in the form of pectin and / or esterified cellulose, and a natural antioxidant in the form of vitamin E and / or antioxidant herbal extracts. The formulation is applied to poultry feed at a dose of 2 to 2.5 g per kg of feed and is able to bind aflatoxin and ochratoxin simultaneously in the digestive tract, thereby reducing the bioavailability of mycotoxins.The application of this formulation has been proven to reduce histopathological damage to the Bursa of Fabricius, including lymphoid follicle depletion, cyst formation, cell necrosis, inflammatory cell infiltration, and tissue fibrosis, while also supporting the immune system function of female broiler chickens. This invention provides an effective, efficient, and easily applicable technical solution in the poultry farming industry to address mixed mycotoxin contamination in feed.

The invention relates to the technical field of silage, and provides a silage additive for degrading mycotoxin and application of the silage additive. The composite microbial inoculant is composed of lactobacillus paracasei and bacillus subtilis; or the microbial agent is a composite microbial agent composed of lactobacillus paracasei, bacillus subtilis and pediococcus pentosaceus. By screening degrading bacteria aiming at ochratoxin, aflatoxin, zearalenone and vomitoxin for compounding and combination, the degrading effects of different lactic acid bacteria on various toxins are respectively exerted, the various mycotoxins are effectively degraded, and particularly, the degrading rate of the ochratoxin can reach 100%. The degradation rate of the mixed bacteria to other mycotoxins can exceed that reported in the prior art, and faster degradation or higher degradation rate can be achieved by adjusting the addition amount.

The invention belongs to the field of analytical chemistry, and particularly relates to an electrochemical luminescence ochratoxin A sensing platform based on an enzyme-free catalysis DNA nano walker, a preparation method and application. According to the invention, the gold nanocluster is loaded on the silver nanomaterial for the first time to realize high electrochemical luminescence performance, the stability and sensitivity of an ochratoxin A sensing platform are improved, and meanwhile, an enzyme-catalysis-free nano walker is designed for amplifying DNA to realize specific analysis of low-concentration ochratoxin A.

The invention discloses a group of ochratoxin A degrading enzyme efficient mutants and application thereof. The amino acid sequence of the high-efficiency mutant of the ochratoxin A degrading enzyme is as shown in SEQ ID NO. 3 or SEQ ID NO. 5. And the preferable nucleotide sequence is as shown in SEQ ID NO. 4 or SEQ ID NO. 6. The kcat / Km value of the mutant reaches 26.8 times and 72.9 times of the kcat / Km value of a wild type respectively and is 0.061982 s <-1 > mu M <-1 > and 0.168442 s <-1 > mu M <-1 > respectively, the OTA catalytic efficiency is remarkably enhanced, the OTA degradation efficiency is improved, and the mutant has important significance on food safety problems and detoxification in food processing.

The invention discloses a screening method and application of natural small molecules for inhibiting growth of Aspergillus carbonniger and synthesis of ochratoxin A, and relates to the technical field of bioinformatics. The invention particularly provides an application of coumarin or coumarin derivatives in preparation of a preparation for inhibiting growth of aspergillus carbonus niger and / or synthesis of ochratoxin A. The invention further provides a preparation method of the coumarin or the coumarin derivatives. According to the invention, SE of Aspergillus carbonus is taken as a target spot, small molecules with potential inhibitory activity are rapidly screened from a natural compound library by using a virtual screening technology, and experiments prove that coumarin and specific derivatives thereof not only can effectively inhibit the growth of Aspergillus carbonus, but also can efficiently inhibit the biosynthesis of OTA, so that the coumarin and specific derivatives thereof can be used for preparing drugs for inhibiting OTA. A theoretical basis and technical support are provided for the application of the strain in the postharvest preservation of agricultural products.

The invention discloses application of amide hydrolase OtaH in degradation of ochratoxin A, and relates to the technical field of enzyme engineering and food safety biology. The amino acid sequence of the amide hydrolase OtaH is as shown in SEQ ID NO.2, and the coding gene sequence of the amide hydrolase OtaH is as shown in SEQ ID NO.1. The invention provides the application of the amide hydrolase OtaH in degradation of ochratoxin A. The invention further provides the application of the amide hydrolase OtaH in degradation of ochratoxin A. The amide hydrolase OtaH can specifically hydrolyze an amido bond of ochratoxin A (OTA) to generate almost non-toxic ochratoxin alpha, has high catalytic efficiency, can completely degrade 12.4 mu M OTA within 5 minutes in the enzyme amount of 5.0 mu g / mL, still keeps more than 50% of activity after being incubated for 1 hour at 65 DEG C, shows excellent thermal stability, can be widely applied to detoxification treatment of OTA in feed and food, and has a wide application prospect in the treatment of ochratoxin alpha, ochratoxin alpha, ochratoxin alpha, ochratoxin alpha, ochratoxin alpha, ochratoxin alpha, ochratoxin alpha, ochratoxin alpha, ochratoxin alpha, ochratoxin alpha and ochratoxin alpha. The harm of OTA to human beings and animals is reduced.

The application discloses a hybrid synergistic electrochemiluminescence quencher, a preparation method and application thereof. Dopamine, zinc ions and 2-methyl imidazole are used as raw materials to synthesize polydopamine and a hybrid material of a zeolitic imidazolate framework by one-pot synthesis under alkaline conditions. The introduction of the electropositive zeolitic imidazolate framework can greatly eliminate the strong electronegativity of the polydopamine and simultaneously endow the quencher with good porosity. The quencher prepared by the application is nanospheres, has a large specific surface area and weak electronegativity, and exhibits good scavenging capacity for free radicals, especially electrogenerated anionic radicals, and has a high quenching efficiency on electrochemiluminescence. After the quencher is labeled with an antibody, the quencher is used for constructing an electrochemiluminescence immunosensor for ochratoxin A. The electrochemiluminescence immunosensor prepared by the application has a wide detection range, a low detection limit and good stability in the detection of ochratoxin A, and has a wide application prospect in the fields of electrochemiluminescence technology and the like.

The invention discloses an ochratoxin compound with aquatic pathogenic bacterium resisting activity and a preparation method and application thereof, the ochratoxin compound is characterized in that the structural formula of the compound is shown as I, and the preparation method comprises the following steps: firstly, obtaining a fermentation product of marine fungi through fermentation culture; soaking the fermented product with ethyl acetate, and extracting to obtain a crude extract; the preparation method comprises the following steps: firstly, preparing a crude extract, then separating and purifying the crude extract by using normal-phase silica gel column chromatography, reverse medium-pressure column chromatography and reversed-phase semi-preparative high performance liquid chromatography to finally obtain the target ochratoxin, and the target ochratoxin has the advantages that the target ochratoxin has a remarkable effect of resisting the activity of aquatic pathogenic bacteria and can be used for research and development of drugs for treating aquatic mycosis.

This invention concerns a mycotoxin detoxification-based feed additive for poultry, particularly broiler chickens, aimed at addressing the decline in feed nutrient utilization and performance due to aflatoxin B1 and ochratoxin A contamination. This invention relates to the use of a multicomponent feed additive formulation that combines inorganic mycotoxin binders, mycotoxin modifying components in the form of biological agents and / or detoxification enzymes, and supporting components in the form of natural antioxidants that work synergistically through adsorption and biotransformation mechanisms in the digestive tract. This formulation functions to reduce the toxicity of aflatoxin B1 and ochratoxin A thereby optimizing the utilization of feed nutrients. The application of this feed additive at an effective dose allows for increased feed utilization efficiency and performance of broiler chickens consuming mycotoxin-contaminated feed, as well as supporting livestock health and productivity in a practical and efficient manner.This invention can be applied in poultry feed management, especially in conditions with a high risk of mycotoxin contamination.

The invention discloses an ochratoxin A hapten, an ochratoxin A artificial antigen, an ochratoxin A antibody and a preparation method and application thereof, and relates to the technical field of immunochemistry and biotoxin detection. The hapten is prepared from ochratoxin A and succinic anhydride through reaction, the artificial antigen is prepared from the hapten and isobutyl chloroformate, and the antibody is prepared from animals immunized by the artificial antigen. The antigen and the antibody can be applied to preparation of an ochratoxin A colloidal gold immunochromatography test strip, and can rapidly and sensitively detect ochratoxin A residues in animal-derived food.

The application discloses an antibody against ochratoxin A and application thereof. The application uses an artificial antigen obtained by coupling ochratoxin A and a carrier protein as an immunogen to prepare a monoclonal antibody against ochratoxin A. The monoclonal antibody can sensitively and specifically detect ochratoxin A, and the minimum detection limit (LOD) is 0.18 ng / mL. In addition, the half inhibition (IC 50 ) of the icELISA for ochratoxin A established by using the monoclonal antibody against ochratoxin A is 1.42 ng / mL, and the linear range is 0.34-5.40 ng / mL. That is, the application provides an antibody capable of detecting ochratoxin A and a method for detecting ochratoxin A, and has the advantages of simplicity, rapidness, strong specificity, wide linear range and high sensitivity.

An ochratoxin A electrochemical sensor for driving DNAzyme to release AgNCs through a primer exchange reaction. The present application belongs to the technical field of biosensors and provides an ochratoxin A electrochemical sensor, which comprises: an A-P probe, a gold electrode modified with capture DNA (cDNA), AgNCs modified with hairpin H, hairpin H1, hairpin H2, KF DNA polymerase, a mixture of deoxynucleotides dATP, dCTP and dGTP, Mg 2+ , H2O2; the A-P probe is obtained by hybridization of an aptamer Apt and a primer P; the nucleotide sequences of the aptamer Apt, the primer P, the capture DNA, the hairpin H, the hairpin H1 and the hairpin H2 are shown in SEQ ID NO: 1-6. The sensor has a simple preparation method, stable performance and is suitable for detection of OTA in food and environment; the process cost of the preparation process is low, the performance is stable, the electrode has good repeatability and is suitable for the requirement of low cost in industrialization.

The invention discloses an electrochemical luminescence immunosensor for detecting ochratoxin A (OTA), and belongs to the technical field of biological detection. The sensor comprises an electrochemical luminescence probe and an electrochemical luminescence electrode, wherein the probe is prepared by coupling Tb-MOF subjected to carboxyl activation with an OTA antibody; the electrode is prepared by modifying Au-Ag NPs and then incubating with an OTA coating antigen. According to the invention, qualitative or quantitative analysis of OTA is realized by detecting the intensity of an electrochemical luminescence signal based on a competitive immunoreaction principle. The sensor has the advantages of being high in sensitivity, easy and convenient to operate, low in background signal, wide in linear range (0.0001-1000 ng / mL) and the like, is suitable for rapid detection of OTA in actual samples such as grains and feed, has the recovery rate of 92%-104%, and has good application prospects.

The present application belongs to the technical field of rapid detection of food and agricultural products, and particularly relates to a method for detecting mycotoxins by using multifunctional portable electrochemical technology. First, tetrahedral DNA specifically recognizing ochratoxin A and tetrahedral DNA specifically recognizing aflatoxin B1 are prepared, and then mixed and modified on a gold nanoflower screen-printed electrode to obtain an aptamer functionalized electrode. Then, the methylene blue@covalent organic framework modified by the aflatoxin B1 aptamer and the ferrocene@covalent organic framework modified by the ochratoxin A aptamer are mixed and modified on the surface of the aptamer functionalized electrode, and finally a multifunctional electrochemical sensor is obtained. The electrochemical sensor can be connected with a portable electrochemical workstation to form a portable electrochemical detection device, which can realize on-site collection and processing of electrochemical signals, and further realize rapid qualitative identification and quantitative detection of ochratoxin A and aflatoxin B1 in food, and is easy to operate and has a wide application prospect.

The invention discloses a thermostable ochratoxin A (OTA) degrading enzyme as well as application, a method and a product thereof, and belongs to the technical field of microorganisms. After OTA degrading enzyme from a thermophilic microorganism Rhodotherm marinus is optimized, enzyme capable of effectively catalyzing ochratoxin A to be degraded into non-toxic substances is obtained through escherichia coli heterologous expression and purification, the specific activity of the enzyme reaches 6666 U / mg, the half-life period at 90 DEG C reaches 7.91 min, and the enzyme is superior to all known OTA detoxification enzymes. The OTA degrading enzyme successfully solves the technical problems that the OTA degrading enzyme in the prior art is relatively poor in stability and cannot meet relatively harsh industrial environmental conditions.

The application provides a probe for detecting ochratoxin A, characterized in that the probe comprises an aptamer of ochratoxin A and two fluorescent dye labels, the fluorescent dye labels are located at two ends of the aptamer, and the sequence of the aptamer is GAT CGG GTG TGG GTG GCG TAA AGG GAG CAT CT x wherein X is an integer selected from 0 to 10, and the two fluorescent dye labels can occur fluorescence resonance energy transfer when close in space. The application also provides a detection method of ochratoxin A. The detection method has many advantages, is carried out in a homogeneous solution, does not need separation, does not need a fixed molecule, is fast in detection, stable in signal, simple in operation, high in sensitivity and the like.

The invention discloses a brevibacillus parabrevis S09T2 strain and an application of the brevibacillus parabrevis S09T2 strain in degrading ochratoxin A. The brevibacillus parabrevis S09T2 is preserved in the Guangdong Microbial Culture Collection Center on September 26, 2025, and the preservation number of the brevibacillus parabrevis S09T2 is GDMCC No: 67039. The brevibacillus parabrevis S09T2 is preserved in the Guangdong Microbial Culture Collection Center on September 26, 2025. The invention finds that the strain has the capability of efficiently degrading ochratoxin A (OTA) for the first time, and fermentation liquor of the strain can realize the degradation rate of over 93 percent on OTA with the concentration of 5 mu g / mL or 8 mu g / mL within 24 hours. Genome analysis shows that the strain does not contain known virulence genes and drug-resistant genes, has no hemolytic activity and has good biological safety. The further research shows that the intracellular solubles of the strain can significantly degrade OTA in the plumeria rubra lixivium, so that the harm of OTA is reduced, and the strain has a good application prospect.

The invention provides a method for simultaneously determining ochratoxin A and zearalenone in breast milk and application of the method, and belongs to the technical field of food detection. The method comprises the following steps: adding < 13 > C-OTA and < 13 > C-ZEN isotope internal standards into breast milk to be detected, then adding acetonitrile, centrifuging, carrying out purification treatment on supernate by adopting immunomagnetic beads capable of simultaneously purifying ochratoxin A and zearalenone, carrying out nitrogen gas blow-drying and redissolving on the obtained eluent, filtering by virtue of a microfiltration membrane, and collecting the eluent. Detecting by adopting high performance liquid chromatography in series with triple quadrupole mass spectrometry, and substituting a detection result into a linear equation of a standard curve to obtain the concentration of ochratoxin A and zearalenone in the sample to be detected. The method has double functions of enrichment and detection, has the characteristics of high sensitivity and high recovery rate, and provides an efficient and accurate solution for trace pollution detection in breast milk.

The application belongs to the technical field of biosensors and provides an electrochemical biosensor for detecting ochratoxin A based on DNA tetrahedron, which comprises an A-P probe, a gold electrode with a modified capture probe CP, a circular template CT, phi 29 polymerase, dNTPs, Mg 2+ , hemoglobin, K + , H2O2; the A-P probe is obtained by hybridization of an aptamer Apt and a primer P; the nucleotide sequences of the aptamer Apt, the primer P, the capture probe CP and the circular template CT are shown as SEQ ID NO: 1-4; the 5' end of the capture probe CP is modified with SH. The sensor has a simple preparation method, stable performance and is suitable for the detection of OTA in food and environment; the process cost of the preparation process is low, the performance is stable, the electrode has good repeatability and is suitable for the requirements of cheapness in industrialization.

The invention discloses an application of sodium phytate in preparation of a product for relieving chick ochratoxin A poisoning. New application of sodium phytate is found through research, and particularly relates to application of sodium phytate in relieving poisoning symptoms caused by OTA ingestion of chicks, especially liver injury caused by OTA ingestion of the chicks. The sodium phytate can be used as a main component of a medicine, a feed or a feed additive, and the chicks can be fed according to the dosage of 2mg / kg body weight. After chicks intake the OTA-polluted feed, sodium phytate can significantly reduce pathological damage of OTA to chick liver tissues and reduce hepatotoxicity from multiple ways of improving serum biochemical indexes related to chick liver functions, inhibiting oxidative stress and inflammatory response and the like. Therefore, the invention provides a safe, efficient and residue-free solution of natural plant source medicines, feeds or feed additives for preventing and controlling the OTA poisoning of the poultry, and has a good industrial application prospect.

The invention relates to a fluorescence colorimetric dual-mode immunodetection kit based on dopamine hydrochloride and graphene quantum dot mediation and application of the fluorescence colorimetric dual-mode immunodetection kit in ochratoxin A detection. Orange oxDH is generated through catalytic oxidation of DH by using HRP, and colorimetric and fluorescent double-reading detection is realized through an internal filtering effect between oxDH and GQD. The detection limit of the fluorescence'off 'detection mode can reach 0.00329 ng / mL and 0.0446 ng / mL. Compared with a traditional ELISA method, the sensitivity is improved by 1.8-7.6 times. And the detection time is only 20 minutes, so that the detection efficiency is effectively improved. The method shows good selectivity on OTA in common mycotoxins, and shows excellent stability under long-term storage at the same time. The method has a good recovery rate in corn and rice, and a rapid and simple technical means is provided for detection of OTA in the product.

The invention relates to the technical field of traditional Chinese medicine risk assessment, in particular to an ochratoxin A probability risk assessment method and system. The method mainly comprises the following steps: acquiring optimal distribution of annual intake of traditional Chinese medicine and optimal distribution of pollution level; establishing an exposure evaluation model M of the ochratoxin A in the traditional Chinese medicine to be evaluated; obtaining the daily exposure distribution of the ochratoxin A in the traditional Chinese medicine to be evaluated under the non-tumor effect and the tumor effect; and calculating risk entropy RQ according to the daily exposure distribution, comparing the risk entropy RQ with a safety standard value, and evaluating the risk level of the ochratoxin A in the traditional Chinese medicine. According to the method, a risk assessment model about the ochratoxin A in the traditional Chinese medicine is established based on Monte Carlo simulation, the risk assessment accuracy of the ochratoxin A in the traditional Chinese medicine can be greatly improved, and technical support is provided for government and traditional Chinese medicine management institutions to risk management of the ochratoxin A in the traditional Chinese medicine.

The application provides application of an amide bond hydrolase in degrading ochratoxin A, and the amide bond hydrolase has any one of the amino acid sequences shown in (I), (II) or (III): (I) an amino acid sequence shown in SEQ ID NO:1; (II) an amino acid sequence with homology of greater than or equal to 90% to the amino acid sequence shown in SEQ ID NO:1, and an amino acid sequence with the same function or similar function as the sequence shown in (I); (III) an amino acid sequence obtained by modifying, substituting, deleting or adding at least one amino acid of the amino acid sequence shown in SEQ ID NO:1. The amide bond hydrolase has the function of degrading ochratoxin A, and the degradation rate is greater than or equal to 99.09%, and can be used as a detoxicant of ochratoxin A, and has important application value in food processing and food safety guarantee.

The present application relates to the technical field of harmful substance detection, in particular to a fluorescent probe for detecting ochratoxin A and a preparation method and application thereof.The fluorescent probe for detecting ochratoxin A is a complex of a fluorescent dye DOCE and serum albumin combined by hydrogen bonds; wherein the chemical structural formula of the fluorescent dye DOCE is as shown.The fluorescent probe prepared by the present application is used for rapid quantitative or qualitative detection of ochratoxin A (OTA), and the detection method has an ultrafast response speed (5 seconds), high sensitivity (detection limit is 0.39 ppb), and high selectivity, and has been successfully applied to on-site rapid detection of real food samples such as flour, white wine and red wine.