158 results about "Tissue fibrosis" patented technology

Various human monoclonal antibodies that bind to human TGF-β type II receptor to inhibit the signal transduction of human TGF-β signal into cells are produced by immunizing human antibody-producing transgenic mice generated by genetic engineering techniques with the soluble human TGF-β type II receptor. Further, these human monoclonal antibodies were demonstrated to be effective for preventing and treating various diseases induced by human TGF-β in various organs (for example, tissue fibrosis).

The present invention relates to a medicine composition formed from breviscapine which is effective component extracted from Chinese medicinal material erigeron breviscapus and cynarin and its new application for curing acute and chronic hepatitides. The breviscapine and cynarin are mixed according to a certain proportion, and proper auxiliary material can be added, and can be made into various preparations for curing and preventing the diseases of fibrosis of liver, cirrhosis of liver and fatty liver, etc.

The present invention provides compositions and methods for increasing therapeutic gain in radiotherapy and chemotherapy for proliferating malignant or nonmalignant disease to produce high probability of tumor control with low frequency of sequelae of therapy by administering a therapeutically effective amount of a histone deacetylase inhibitor. The compounds are capable of simultaneously stimulating the epithelium regrowth, inhibiting the fibroblast proliferation, decreasing the collagen deposit, suppressing the fibrogenic growth factor, subsiding the proinflammatory cytokine and modulating the expression of cell cycle genes, tumor suppressors and oncogenes, and are useful to increase the therapeutic gain in radiotherapy and chemotherapy, which results in decrease of skin swelling and inflammation, promotion of epithelium healing in mucosa and dermis, decrease of xerostomia, prevention / reduction of severity of plantar-palmar syndrome, prevention of tissue fibrosis, ulceration, necrosis and tumorigenesis, and increase of tumor growth inhibition and tumor therapy effectiveness.

The invention belongs to the technical field of molecular biology and relates to application of an exosome derived from human mesenchymal stem cells to preparation of medicines for antagonizing scars or tissue and organ fibrosis. According to the application, the high-purity and high-bioactivity exosome is obtained by subjecting healthy human umbilical cords to primary in-vitro culture, collecting liquid supernatant after amplification, performing low-temperature ultracentrifugation, purifying and the like; the exosome is used individually or added into medicines, health care products and cosmetics according to a proportion to be developed into a product with a bioactivity function, thereby being capable of regulating organism cell signal transduction effectively, inhibiting fibrocyte differentiation and then antagonizing the tissue and organ fibrosis and scar forming. Through experimental verification, the exosome can play a remarkable role in repairing promotion in hypertrophic scar after skin injury, particularly pathological fibrosis proliferation such as scar contracture of the joint parts, hepatic fibrosis and cardiac fibrosis, and is high in safety, little in toxicity and convenient to storage and transport, thereby being wide in application prospect.

Embodiments of the invention provides methods, devices and kits for determining the likelihood of an individual having an active fibrotic condition and / or early diagnosis, and subsequently prognosis evaluation of an individual having an active fibrotic condition such as systemic sclerosis (SSc) and / or nephrogenic systemic fibrosis (NSF) by measuring the levels of several biomarkers: α-enolase (ENO1), reticulocalbin 3 (RCN-3), alpha smooth muscle actin (α-SMA), reticulocalbin 1 (RCN-1) and pigment epithelium-derived factor (PEDF) in the individual.

Use of an inhibitor of a Hedgehog signalling pathway, or an inhibitor of a pathway which is a target of the Hedgehog signalling pathway in the preparation of a medicament for treatment of epithelial cell hyperplasia, fibrosis of tissue, inflammation, cancer or an immune disorder.

The present invention provides a method of diagnosing the presence or severity of tissue fibrosis in an individual by detecting α2-macroglobulin (α2-MG) in a sample from the individual; detecting hyaluronic acid (HA) in a sample from the individual; detecting tissue inhibitor of metalloproteinases-1 (TIMP-1) in a sample from the individual; and diagnosing the presence or severity of tissue fibrosis in the individual based on the presence or level of α2-MG, HA and TIMP-1.

The present invention provides a method of diagnosing the presence or severity of tissue fibrosis in an individual by detecting α2-macroglobulin (α2-MG) in a sample from the individual; detecting hyaluronic acid (HA) in a sample from the individual; detecting tissue inhibitor of metalloproteinases-1 (TIMP-1) in a sample from the individual; and diagnosing the presence or severity of tissue fibrosis in the individual based on the presence or level of α2-MG, HA and TIMP-1.

The invention relates to a screening method for a medicine for targeting NS5ATP9 (HCV NS5A-transactivated protein 9) prevention or treatment of tissue fibrosis and tissue sclerosis, and the medicine obtained by screening using the method. NS5ATP9 significantly regulates the expression of extracellular matrix collagen and non-collagen glycoprotein and affects the occurrence and development of tissue fibrosis by regulating a TGFbeta1 / Smad3 signaling pathway; and the NS5ATP9 promotes the apoptosis of hepatic stellate cells, inhibits the proliferation of the hepatic stellate cells and regulates extracellular matrix deposition by regulating the phosphorylation level and the intracellular translocation condition of a Smad3 protein. TDF / TAF can effectively regulate the expression of NS5ATP9 gene,and is verified in CCl4-induced mouse liver fibrosis model, and the TDF / TAF achieves significant effects in the prevention or treatment of CCl4-induced liver fibrosis in mice.

The invention relates to the technical field of medicine, in particular to the use of 13-methylamino-18-thiomatrine compound (I) and its salts in the preparation of anti-fibrosis drugs. The present invention proves from cytological tests that 13-methylamino-18-thiomatrine can inhibit proliferation and activation of extracellular matrix producing cells hepatic stellate cells, and inhibit fibrotic liver epithelial cell-mesenchymal transition. In addition, it also has inhibitory effects on various other extracellular matrix-producing cells that lead to organ or tissue fibrosis. Animal experiments have proved that 13-methylamino-18-thiomatrine has a very significant anti-hepatic fibrosis effect. The results show that 13-methylamino-18-thiomatrine has definite anti-hepatic fibrosis or fibrosis of other tissues and organs, and can be used to prepare medicines for preventing and treating liver fibrosis or fibrosis of other tissues and organs.

The invention relates to application of anti-aging gene segments and protein products of the anti-aging gene segments in treating chronic renal failure, in particular to application of secretory type Klotho genes and proteins of the Klotho genes in preparing medicine for treating chronic renal failure. According to experiments, the secretory type Klotho has remarkable effect of restraining development of kidney tissue fibrosis and chronic renal failure and has no obvious side and toxic effects, so that the secretory type Klotho can be used for preparing medicines for treating chronic renal failure and particularly used for preparing medicines capable of effectively restraining and delaying development of chronic renal failure.

The invention provides silk fibroin nanoparticles and drug-loading silk fibroin nanoparticles. The silk fibroin nanoparticles are prepared by a method comprising the following steps: dissolving silk fibroin in water, and keeping the water in which the silk fibroin is dissolved at 2-6 DEG C for 2-8h, so that a silk fibroin solution is obtained; mixing the silk fibroin solution with an organic solvent at the volume ratio of 1 to (4-8), and implementing stirring so as to obtain a nanoparticle suspension, wherein the organic solvent is selected from ethanol, isopropanol or acetone; implementing centrifugal separation on the nanoparticle suspension, washing the nanoparticle suspension by virtue of de-ionized water, and implementing centrifuging, so that a nanoparticle precipitate is obtained; and dispersing the nanoparticle precipitate by virtue of de-ionized water, so that the silk fibroin nanoparticles are obtained. The drug-loading silk fibroin nanoparticles can improve intake of drugs in cells and prolong retention time of the drugs; the drug-loading silk fibroin nanoparticles have the advantages of being effective, long-acting and targeted in cells and the drug-loading silk fibroin nanoparticles are conducive to the improvement of bioavailability of the drugs; the silk fibroin nanoparticles are excellent in biological adhesion and free from immunogenicity; and the silk fibroin nanoparticles are free from obvious inflammatory response and tissue fibrosis, and are excellent in biocompatibility and safety.

The present invention relates to synthetic oligonucleotide mimetics of miRNAs. In particular, the present invention provides double-stranded, chemically-modified oligonucleotide mimetics of miR-29. Pharmaceutical compositions comprising the mimetics and their use in treating or preventing conditions associated with dysregulation of extracellular matrix genes, such as tissue fibrotic conditions, are also described.

The invention belongs to the field of biological medicine, and relates to application of micromolecular polypeptide KP-6, in particular to application of micromolecular polypeptide KP-6 in preparing a drug for treating a chronic kidney disease (CKD). An amino acid sequence of the polypeptide KP-6 is shown as SEQ ID NO. 1. The micromolecular polypeptide KP-6 has a significant effect of inhibiting kidney tissue fibrosis and CKD development, has no obvious toxic or side effects, and therefore can be used for preparing the drug for effectively treating the chronic kidney disease.

The invention discloses a composition of vitamin C and arginine and its application, wherein the weight ratio of vitamin C and arginine is 10:1 - 10:4, the composition can be prepared into liquid or solid dosage forms.

The invention discloses a quantitative fast frequency-locked magnetic resonance imaging method. Data acquisition of magnetic resonance imaging signals corresponding to different spin lock pulses in acontinuous scanning period is completed, and the data fills the same position in K space, so that T1rhomapping images finally obtained corresponding to the different spin lock pulses have high matching degree and can be used for judging tissue fibrosis indexes and diagnosing a tissue edema state; the effects of arrhythmia, uneven breathing and the like on the quality of the T1rhomapping images areminimized, and physical state and ability to suspend breathing and the like of a tester are not strictly required, so that scanning time required to obtain the T1rhomapping images is greatly shortened and the research and promotion of a T1rhomapping imaging technology in the field of clinical medical detection is further developed; in addition, the spin lock pulses alternate in positive and negative phases in adjacent RF excitation pulses, thereby effectively reducing artifacts due to magnetic field uniformity.

The invention discloses a traditional Chinese medicine composition for improving renal functions and restraining renal fibrosis. Active pharmaceutical ingredients of the traditional Chinese medicine composition comprise, by weight, 10-15 parts of the root of red-rooted salvia, 10-60 parts of astragalus membranaceus, 10-30 parts of herba epimedii, 5-30 parts of sinensis and 3-30 parts of prepared rhubarb. Clinical researches show that 168 patients with chronic renal insufficiency are treated by the traditional Chinese medicine composition, a remarkable clinical effect is obtained, clinical symptoms are reduced, the clinical symptoms of some patients are basically eliminated, the renal functions of the patients can be remarkably improved, blood urea nitrogen (BUN) and creatinine (Scr) are obviously reduced, and total effective rate achieves 88.66%. Animal researches show that the traditional Chinese medicine composition can delay development of chronic renal failure, and the mechanism of the traditional Chinese medicine composition is relative to effects of effectively restraining precipitation of renal tissue collagen and secretion of TGF (transforming growth factor)-beta, accordingly restraining renal fibrosis, and the like. The traditional Chinese medicine composition is low in clinical application cost and excellent in curative effect, and is safe and reliable.

The invention discloses a scar and tissue fibration resistant oligomeric double-stranded nucleotide medicine. The sequence of the nucleotide medicine comprises TGACTCA, TTACCTCA, AGCCAGACA and ATGCAGACA nucleic acid sequences or functional equivalents thereof, and the above four core nucleic acid sequences are contained in the sense strand or the antisense strand of the double-stranded decoy nucleotide.

The invention relates to the field of medicaments, in particular to a novel bifunctional cicatrix and tissue fibrosis resistant oligonucleotide medicament. The nucleic acid contains an AP-1 cis-acting component in high affinity binding with AP-1 and a Smad cis-acting component in high affinity binding with Smad.

The invention relates to anti-postoperation scar degradable multi-branched glycopeptide hydrogel and a preparing method thereof. The hydrogel is formed by self-assembly of multi-branched glycopeptides modified with hydrophobic cholesterol under physiological conditions through hydrophobic forces, pi-pi stacking, hydrogen bonds, and other forces. A glucosamine unit in the structure of the multi-branched glycopeptides can effectively inhibit tissue fibrosis caused by fibroblast proliferation so as to prevent formation of a postoperation scar, so that the hydrogel can be used as an anti-postoperation scar gel phase preparation. By introduction of the hydrophobic cholesterol, biocompatibility of the gel is enhanced, toxic and side effects and inflammation risks of the gel are reduced, and the self-assembly capability and stability of the hydrogel are enhanced. The hydrogel has advantages of high biosecurity, convenient operation, and the like.

The invention discloses a copolymerized nano composite hydrogel for intelligent separation of cell sheets as well as a preparation method and application of the copolymerized nano composite hydrogel. The preparation method comprises the steps of: dispersing laponite in water, stirring to obtain homogenous and transparent dispersion, then sequentially adding polyethylene glycol macromonomer and N-isopropyl acrylamide monomer, uniformly stirring, removing oxygen, adding an initiator, transferring reaction liquid to a glass test tube and a reaction mold, sealing, and carrying out in-situ radicalpolymerization reaction at 15-25 DEG C to obtain a target product. The prepared copolymerized nano composite hydrogel has good mechanical performance and temperature sensitivity, and can be used for cell culture and rapid desorption of the cell sheets. The copolymerized nano composite hydrogel is suitable for cell growth, and can also realize rapid and automatic desorption of the cell sheets fromthe surface of the hydrogel by reducing the ambient temperature of the gel, thus tissue fibrosis and inflammation generation caused by use of biodegrable supports are avoided, and the damage to extracellular matrix and intercellular connection caused by use of trypsogen is avoided.

The present invention relates to synthetic oligonucleotide mimetics of miRNAs. In particular, the present invention provides double-stranded, chemically-modified oligonucleotide mimetics of miR-29. Pharmaceutical compositions comprising the mimetics and their use in treating or preventing conditions associated with dysregulation of extracellular matrix genes, such as tissue fibrotic conditions, are also described.

The invention relates to protecting functions and applications of FSTL1 in anti-fibrosis steady-state regulation of tissues such as livers, and particularly relates to uses of FSTL1 protein or an FSTL1 regulating compound in preparation of medicines treating tissue fibrosis diseases. The invention also relates to a conditional FSTL1 gene knockout non-human animal model closely related to the degree of tissue fibrosis pathological changes, a preparing method thereof and uses of the model in tissue-fibrosis-preventing medicine screening. Treatment is performed by combining different tamoxifen dosages and different animal growth stages, the systemic or vascular endothelial cell FSTL1 gene knockout non-human animal model is obtained and the model is used for simulating different tissue fibrosis pathological changes.

A pharmaceutical composition having an inhibitory effect on the overproduction and the accumulation of extracellular matrix, said composition comprising as an active ingredient a compound that inhibits the biological activity of galectin-3, which pharmaceutical composition can serve as a therapeutic or preventive agent for glomerular nephritis, diabetic nephropathy or tissue fibrosis, as well as the use of said compound for the production of pharmaceuticals for the above-mentioned use, and a method for inhibition of the overproduction and accumulation of the extracellular matrix.

Compositions and methods useful for delivery of targeted therapies for pulmonary arterial hypertension, sepsis, cancer and cachexia. The compositions and methods are based on peptide pharmacophores that selectively bind to and home to diseased tissue and enable targeted therapies to affect a beneficial therapeutic result. Peptide pharmacophores may selectively target tumor vasculature, regenerating tissue, wounded tissue, inflamed tissue, fibrotic tissue, remodeled tissue, tissue characterized by elevated heparanase levels, and have the ability to internalize into such diseased cells.

The invention discloses a purpose of curcumin or a drug-loading system thereof in preparation of the drug for treating penis erection dysfunction. An experiment proves that the curcumin and a curcuminslow release drug film can up-regulate the expression of GAP-43 and MAP-2 to accelerate growth of the nerve axon and can up-regulate the expression of Oct-6 and Krox-20 to accelerate Schwann cells tosynthesize and secrete myelin sheath-associated protein so as to accelerate formation of the nerve axon myelin sheath. Meanwhile, repair and regeneration after a rat suffers from cavernous body nerveinjury, neural pathway continuity of the cavernous body is recovered, and expression of penis tissue nerve terminal nNOS mRNA and protein is increased. The penis erection function of the rat who suffers from the cavernous body nerve injury can be further improved, meanwhile, a penis tissue fibrosis degree is lowered, and rehabilitation of the penis after the cavernous body nerve injury is effectively accelerated. Therefore, the curcumin and the drug-loading system thereof perform a good application prospect in the preparation of the drug for treating the penis erection dysfunction and repairing the cavernous body nerve injury.

Methods are provided for treating fibrosis of a tissue, including fibrosis of the liver, using combinations of antagomirs and / or locked nucleic acids. Compositions therefor are also provided.

The present invention provides a kind of composition comprising aweto or its fermented culture 20-80 wt% and cryptoporu volvatus or its fermented culture 20-80 wt%. The composition can inhibit fibrosis of lung and fibrosis of cardiac and cerebral vascular tissue, and possesses tissue fibrosis inhibiting effect obviously higher than that of available medicine interferon gamma. The composition has synergistic tissue fibrosis inhibiting effects of both aweto and cryptoporu volvatus and has no any toxic side effect found.

The present invention relates to synthetic oligonucleotide mimetics of miRNAs. In particular, the present invention provides double-stranded, chemically-modified oligonucleotide mimetics of miR-29. Pharmaceutical compositions comprising the mimetics and their use in treating or preventing conditions associated with dysregulation of extracellular matrix genes, such as tissue fibrotic conditions, are also described.

The invention relates to composition for drug targets and an application. The composition comprises a carrier and a drug adopting FKBP10 and PCOLCE genes and / or protein encoded by the genes as drug targets. By means of intervention in FKBP10 and PCOLCE, formation of tissue fibrosis is inhibited, and biological behaviors such as skin fibroblast proliferation, differentiation, apoptosis resistance,secretion of a large quantity of extracellular matrixes and the like are inhibited, so that the effect of treating fibrosis related diseases is achieved.