The invention provides a thyroid cancer molecular diagnosis kit. The kit comprises detection reagents for at least three genes of the follows: CK19, survivin, TG, LGALS3, MET, TFF3, SERPINA1, TIMP1, FN1, TPO, TGFA, QPCT, CRABP1, FCGBP, EPS8, PROS1, LRP4, DPP4, GJB3, ST14, EVA1, SPUVE, HBB, MKRN2, MRC2, IGSF1, KIAA0830, RXRG, P4HA2, CDH3, IL13RA1, MTMR4, MDK, CITED1, CHI3L1, ODZ1, N33, SFTPB and SCEL. The benign and malignant of thyroid nodule is judged through the expression level of thyroid cancer tissue specific expression genes, and the operation is simple and convenient.