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The invention discloses citrullinated T200C polypeptide and application thereof in preparation of rheumatoid arthritis diagnosis products, and belongs to the technical field of disease diagnosis. The amino acid sequence of the citrullinated T200C polypeptide is as follows: GSM-Cit-GA-Cit-Cit-EPEEPEGAV, and the amino acid sequence of the citrullinated T200C polypeptide is as follows. Researches find that the anti-citrullinated T200C polypeptide antibody level in the body of an RA patient is obviously higher than that of healthy people and other rheumatism immune disease patients easily confused with RA. When the citrullinated T200C polypeptide is used as a detection antigen to detect an anti-citrullinated T200C polypeptide antibody in the body of a patient, RA can be effectively identified, and particularly, a relatively good diagnostic value is achieved for an anti-CCP antibody and / or RF negative RA and ESR and / or CRP normal RA.